General Information of Target

Target ID LDTP12958
Target Name ER membrane protein complex subunit 3 (EMC3)
Gene Name EMC3
Gene ID 55831
Synonyms
TMEM111; ER membrane protein complex subunit 3; Transmembrane protein 111
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSLRVLGSGTWPSAPKMFLLLTALQVLAIAMTQSQEDENKIIGGHTCTRSSQPWQAALLA
GPRRRFLCGGALLSGQWVITAAHCGRPILQVALGKHNLRRWEATQQVLRVVRQVTHPNYN
SRTHDNDLMLLQLQQPARIGRAVRPIEVTQACASPGTSCRVSGWGTISSPIARYPASLQC
VNINISPDEVCQKAYPRTITPGMVCAGVPQGGKDSCQGDSGGPLVCRGQLQGLVSWGMER
CALPGYPGVYTNLCKYRSWIEETMRDK
Target Bioclass
Transporter and channel
Family
EMC3 family
Subcellular location
Endoplasmic reticulum membrane
Function
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
Uniprot ID
Q9P0I2
Ensemble ID
ENST00000245046.7
HGNC ID
HGNC:23999

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Insertion: p.T91NfsTer2 .
KYSE510 SNV: p.M111I .
MCC13 SNV: p.M251I .
NCIH1793 SNV: p.D41H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Jackson_1
 Probe Info 
10.24  LDD0122  [1]
STPyne
 Probe Info 
K253(10.00)  LDD0277  [2]
Jackson_14
 Probe Info 
2.50  LDD0123  [1]
Acrolein
 Probe Info 
N.A.  LDD0221  [3]
ATP probe
 Probe Info 
K253(0.00); K254(0.00)  LDD0199  [4]
Ox-W18
 Probe Info 
N.A.  LDD2175  [5]
PAL-AfBPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info 
5.86  LDD1733  [6]
C094
 Probe Info 
46.21  LDD1785  [6]
C235
 Probe Info 
24.08  LDD1908  [6]
C278
 Probe Info 
53.45  LDD1948  [6]
C289
 Probe Info 
29.45  LDD1959  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0107  IAA HeLa N.A.  LDD0221  [3]
 LDCM0109  NEM HeLa N.A.  LDD0225  [3]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 2.50  LDD0123  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
S-methyl-5'-thioadenosine phosphorylase (MTAP) PNP/MTAP phosphorylase family Q13126
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ER membrane protein complex subunit 2 (EMC2) EMC2 family Q15006

References

1 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587