Details of the Target

General Information of Target

Target ID LDTP11229
Target Name Transmembrane protein 70, mitochondrial (TMEM70)
Gene Name TMEM70
Gene ID 54968
Synonyms
Transmembrane protein 70, mitochondrial
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAY
AKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRF
YLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPS
GLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMK
LNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPP
FVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAK
QRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENEL
AEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
Target Bioclass
Transporter and channel
Family
TMEM70 family
Subcellular location
Mitochondrion inner membrane
Function
Scaffold protein that participates in the c-ring assembly of mitochondrial ATP synthase (F(1)F(0) ATP synthase or complex V) by facilitating the membrane insertion and oligomer formation of the subunit c/ATP5MC1 through its interaction. Therefore, participates in the early stage of mitochondrial ATP synthase biogenesis and also protects subunit c/ATP5MC1 against intramitochondrial proteolysis. In addition, binds the mitochondrial proton-transporting ATP synthase complexes I and may play a role in the stability of its membrane-bound subassemblies.
Uniprot ID
Q9BUB7
Ensemble ID
ENST00000312184.6
HGNC ID
HGNC:26050

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.M187K .
HEC1 SNV: p.S35A .
HEC1B SNV: p.S35A .
KYSE30 SNV: p.H196Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		9.35  LDD0402  [1]
N1
 Probe Info  		100.00  LDD0242  [2]
STPyne
 Probe Info  		K206(5.81)  LDD2217  [3]
EA-probe
 Probe Info  		N.A.  LDD0440  [4]
ATP probe
 Probe Info  		N.A.  LDD0199  [5]
Acrolein
 Probe Info  		N.A.  LDD0217  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]
PAL-AfBPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		5.78  LDD1733  [8]
C094
 Probe Info  		31.12  LDD1785  [8]
C106
 Probe Info  		19.16  LDD1793  [8]
C108
 Probe Info  		8.46  LDD1795  [8]
C112
 Probe Info  		21.86  LDD1799  [8]
C161
 Probe Info  		9.78  LDD1841  [8]
C187
 Probe Info  		17.51  LDD1865  [8]
C228
 Probe Info  		19.43  LDD1901  [8]
C289
 Probe Info  		36.25  LDD1959  [8]
C350
 Probe Info  		25.28  LDD2011  [8]
C362
 Probe Info  		30.70  LDD2023  [8]
C388
 Probe Info  		37.53  LDD2047  [8]
FFF probe11
 Probe Info  		20.00  LDD0472  [9]
FFF probe14
 Probe Info  		16.69  LDD0477  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.70  LDD0403  [1]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [4]
 LDCM0109  NEM HeLa N.A.  LDD0227  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ras-related protein Rab-2A (RAB2A) Rab family P61019
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lysophosphatidic acid receptor 3 (LPAR3) G-protein coupled receptor 1 family Q9UBY5

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
4 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
9 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.