Details of the Target

General Information of Target

Target ID LDTP16258
Target Name N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL)
Gene Name PIGL
Gene ID 9487
Synonyms
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase; EC 3.5.1.89; Phosphatidylinositol-glycan biosynthesis class L protein; PIG-L
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MASVALEDVAVNFTREEWALLGPCQKNLYKDVMQETIRNLDCVVMKWKDQNIEDQYRYPR
KNLRCRMLERFVESKDGTQCGETSSQIQDSIVTKNTLPGVGPCESSMRGEKVMGHSSLNC
YIRVGAGHKPHEYHECGEKPDTHKQRGKAFSYHNSFQTHERLHTGKKPYDCKECGKSFSS
LGNLQRHMAVQRGDGPYKCKLCGKAFFWPSLLHMHERTHTGEKPYECKQCSKAFSFYSSY
LRHERTHTGEKPYECKQCSKAFPFYSSYLRHERTHTGEKPYKCKQCSKAFPDSSSCLIHE
RTHTGEKPYTCKQCGKAFSVSGSLQRHETTHSAEKPYACQQCGKAFHHLGSFQRHMIRHT
GNGPHKCKICGKGFDCPSSLQSHERTHTGEKPYECKQCGKALSHRSSFRSHMIMHTGDGP
HKCKVCGKAFVYPSVFQRHERTHTAEKPYKCKQCGKAYRISSSLRRHETTHTGEKPYKCK
LGKACIDFCSFQNHKTTHTGEKPYECKECGKAFSRFRYLSRHKRTHTGEKPYECKTCRKA
FGHYDNLKVHERIHSGEKPYECKECGKAFSWLTCFLRHERIHMREKSYECPQCGKAFTHS
RFLQGHEKTHTGENPYECKECGKAFASLSSLHRHKKTHWKKTHTGENPYECKECGKAFAS
LSSLHRHKKTH
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
PIGL family
Subcellular location
Endoplasmic reticulum membrane
Function Catalyzes the second step of glycosylphosphatidylinositol (GPI) biosynthesis, which is the de-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol.
TTD ID
T27747
Uniprot ID
Q9Y2B2
DrugMap ID
TTQA8DT
Ensemble ID
ENST00000225609.10
HGNC ID
HGNC:8966

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DANG Deletion: p.Q84_G85delinsR .
NCIH2172 SNV: p.R227L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C225(6.40)  LDD1702  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
PAL-AfBPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		5.58  LDD1733  [3]
C056
 Probe Info  		21.71  LDD1753  [3]
C063
 Probe Info  		10.70  LDD1760  [3]
C064
 Probe Info  		8.28  LDD1761  [3]
C112
 Probe Info  		37.53  LDD1799  [3]
C285
 Probe Info  		20.97  LDD1955  [3]
C289
 Probe Info  		38.05  LDD1959  [3]
C388
 Probe Info  		71.01  LDD2047  [3]
C407
 Probe Info  		14.52  LDD2064  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C225(6.40)  LDD1702  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587