Probe Name	C260					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP16040	Glyoxalase domain-containing protein 4 (GLOD4)					26.7
LDTP16007	Lipid droplet-associated hydrolase (LDAH)					26.7
LDTP12013	dCTP pyrophosphatase 1 (DCTPP1)					14.3
LDTP11273	N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1)					14.3
LDTP09088	Phospholipase A2 group XV (PLA2G15)					12.9
LDTP04004	Lysosomal Pro-X carboxypeptidase (PRCP)					10.6
LDTP15988	Probable serine carboxypeptidase CPVL (CPVL)					10.3
LDTP00988	Protein O-GlcNAcase (OGA)					9.0
LDTP01769	Dihydrofolate reductase (DHFR)					7.2
LDTP13282	Prenylcysteine oxidase 1 (PCYOX1)					6.6
LDTP06648	Lanosterol 14-alpha demethylase (CYP51A1)					6.1
LDTP09256	Putative phospholipase B-like 2 (PLBD2)					6.0
LDTP03608	RAC-beta serine/threonine-protein kinase (AKT2)					5.9
LDTP04008	Endothelin-converting enzyme 1 (ECE1)					5.7
LDTP04183	Lanosterol synthase (LSS)					5.7
LDTP01241	Bis(monoacylglycero)phosphate synthase CLN5 (CLN5)					5.5
LDTP00697	Cytochrome b5 type B (CYB5B)					5.5
LDTP06200	Delta(14)-sterol reductase LBR (LBR)					5.4
LDTP03257	Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2)					5.2
LDTP06375	Delta(24)-sterol reductase (DHCR24)					5.2
LDTP07057	Protein phosphatase 1L (PPM1L)					5.2
LDTP13358	Leucyl-cystinyl aminopeptidase (LNPEP)					5.0
LDTP02359	Heme oxygenase 1 (HMOX1)					4.9
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					4.9

Target ID	Target Name					Binding Ratio
LDTP10913	Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A)					18.9
LDTP01804	ATP synthase subunit a (MT-ATP6)					15.1
LDTP07885	Mitochondrial S-adenosylmethionine carrier protein (SLC25A26)					15.1
LDTP05900	Metaxin-1 (MTX1)					11.4
LDTP03277	ER lumen protein-retaining receptor 1 (KDELR1)					11.2
LDTP09547	Scavenger receptor class B member 1 (SCARB1)					10.4
LDTP07236	Protein GPR107 (GPR107)					10.2
LDTP07462	MFS-type transporter SLC18B1 (SLC18B1)					8.9
LDTP17641	Solute carrier family 35 member F2 (SLC35F2)					8.3
LDTP01059	Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B)					6.6
LDTP15948	Transmembrane protein 126A (TMEM126A)					6.5
LDTP06426	Translocating chain-associated membrane protein 1 (TRAM1)					6.3
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					6.0
LDTP06945	Sigma intracellular receptor 2 (TMEM97)					5.9
LDTP14200	Mitochondrial ornithine transporter 1 (SLC25A15)					5.4
LDTP00592	Surfeit locus protein 4 (SURF4)					5.4
LDTP09968	V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1)					5.2
LDTP02724	Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2)					5.1
LDTP04033	Translocon-associated protein subunit alpha (SSR1)					4.9

Target ID	Target Name					Binding Ratio
LDTP01447	PHD finger protein 14 (PHF14)					5.9
LDTP12507	Chromatin accessibility complex protein 1 (CHRAC1)					5.2

Target ID	Target Name					Binding Ratio
LDTP12565	Mitochondrial import receptor subunit TOM22 homolog (TOMM22)					10.0
LDTP03678	ER lumen protein-retaining receptor 2 (KDELR2)					9.9
LDTP11076	Apolipoprotein L2 (APOL2)					8.2
LDTP13828	Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3)					7.0
LDTP15714	Mdm2-binding protein (MTBP)					5.9
LDTP14486	Clustered mitochondria protein homolog (CLUH)					5.7
LDTP02156	Eukaryotic translation initiation factor 4E (EIF4E)					5.7
LDTP13755	Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2)					5.3

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587