Probe Name	FFF probe3					Probe Info
Probe concentration	Probe:20uM; negative probe:20uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	SILAC
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP05476	3-ketodihydrosphingosine reductase (KDSR)					20.0
LDTP08476	5'-3' exonuclease PLD3 (PLD3)					20.0
LDTP00150	Acyl-coenzyme A diphosphatase NUDT19 (NUDT19)					20.0
LDTP06905	Acylglycerol kinase, mitochondrial (AGK)					20.0
LDTP04689	Adenosine kinase (ADK)					20.0
LDTP04437	Aldehyde dehydrogenase family 3 member A2 (ALDH3A2)					20.0
LDTP01687	Apoptosis-inducing factor 1, mitochondrial (AIFM1)					20.0
LDTP06618	ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP)					20.0
LDTP02216	Beta-hexosaminidase subunit beta (HEXB)					20.0
LDTP03257	Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2)					20.0
LDTP02198	Cathepsin D (CTSD)					20.0
LDTP00697	Cytochrome b5 type B (CYB5B)					20.0
LDTP12013	dCTP pyrophosphatase 1 (DCTPP1)					20.0
LDTP06375	Delta(24)-sterol reductase (DHCR24)					20.0
LDTP05724	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1)					20.0
LDTP06150	DNA replication licensing factor MCM6 (MCM6)					20.0
LDTP13702	E3 ubiquitin-protein ligase TRIM33 (TRIM33)					20.0
LDTP04008	Endothelin-converting enzyme 1 (ECE1)					20.0
LDTP02175	Epoxide hydrolase 1 (EPHX1)					20.0
LDTP13751	Exonuclease 1 (EXO1)					20.0
LDTP03198	Ferrochelatase, mitochondrial (FECH)					20.0
LDTP02359	Heme oxygenase 1 (HMOX1)					20.0
LDTP03540	Heme oxygenase 2 (HMOX2)					20.0
LDTP04581	Holocytochrome c-type synthase (HCCS)					20.0
LDTP04211	Isocitrate dehydrogenase [NADP], mitochondrial (IDH2)					20.0
LDTP06648	Lanosterol 14-alpha demethylase (CYP51A1)					20.0
LDTP04183	Lanosterol synthase (LSS)					20.0
LDTP09188	Lysophosphatidylcholine acyltransferase 1 (LPCAT1)					20.0
LDTP07504	Lysophospholipid acyltransferase 5 (LPCAT3)					20.0
LDTP04004	Lysosomal Pro-X carboxypeptidase (PRCP)					20.0
LDTP00886	Nardilysin (NRDC)					20.0
LDTP04051	Nicotinamide phosphoribosyltransferase (NAMPT)					20.0
LDTP13282	Prenylcysteine oxidase 1 (PCYOX1)					20.0
LDTP15988	Probable serine carboxypeptidase CPVL (CPVL)					20.0
LDTP06750	Prolyl 3-hydroxylase 1 (P3H1)					20.0
LDTP08979	Prostaglandin reductase 2 (PTGR2)					20.0
LDTP03419	Proteasome subunit beta type-5 (PSMB5)					20.0
LDTP04992	Ras-related protein Rab-11A (RAB11A)					20.0
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					20.0
LDTP06384	Ribosomal protein S6 kinase alpha-1 (RPS6KA1)					20.0
LDTP04471	Ribosomal protein S6 kinase alpha-3 (RPS6KA3)					20.0
LDTP05066	Signal peptidase complex catalytic subunit SEC11A (SEC11A)					20.0
LDTP03842	Squalene synthase (FDFT1)					20.0
LDTP06455	Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL)					20.0
LDTP12056	Thiol S-methyltransferase TMT1A (TMT1A)					20.0
LDTP02522	60 kDa heat shock protein, mitochondrial (HSPD1)					19.9
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					18.5
LDTP04388	Palmitoyl-protein thioesterase 1 (PPT1)					18.2
LDTP06501	Ras-related protein Rab-11B (RAB11B)					18.2
LDTP00186	Alkyldihydroxyacetonephosphate synthase, peroxisomal (AGPS)					18.1
LDTP05632	Mitochondrial-processing peptidase subunit alpha (PMPCA)					17.9
LDTP02622	Creatine kinase U-type, mitochondrial (CKMT1A; CKMT1B)					17.8
LDTP06810	Mitochondrial import inner membrane translocase subunit TIM50 (TIMM50)					17.7
LDTP04892	Ras-related protein Rab-2A (RAB2A)					17.0
LDTP01328	CAAX prenyl protease 1 homolog (ZMPSTE24)					17.0
LDTP01333	Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1)					16.7
LDTP09886	Gamma-glutamyl hydrolase (GGH)					16.5
LDTP04285	Proteasome subunit beta type-2 (PSMB2)					16.3
LDTP07003	Presequence protease, mitochondrial (PITRM1)					16.0
LDTP04398	Ras-related protein Rab-5C (RAB5C)					15.5
LDTP09396	Minor histocompatibility antigen H13 (HM13)					15.5
LDTP01773	Cytochrome c oxidase subunit 2 (MT-CO2)					15.3
LDTP09110	NAD(P)H-hydrate epimerase (NAXE)					15.1
LDTP05588	RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2)					14.9
LDTP13358	Leucyl-cystinyl aminopeptidase (LNPEP)					14.7
LDTP03189	Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2)					14.6
LDTP05006	Ras-related protein Rab-1A (RAB1A)					14.6
LDTP06901	Very-long-chain 3-oxoacyl-CoA reductase (HSD17B12)					14.5
LDTP05758	S-methyl-5'-thioadenosine phosphorylase (MTAP)					14.2
LDTP10329	Ceramide synthase 2 (CERS2)					14.1
LDTP02837	Beta-galactosidase (GLB1)					13.8
LDTP12867	Very-long-chain enoyl-CoA reductase (TECR)					13.7
LDTP03139	Succinate dehydrogenase iron-sulfur subunit, mitochondrial (SDHB)					13.6
LDTP11321	Acetyl-CoA acetyltransferase, cytosolic (ACAT2)					13.4
LDTP02860	Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2)					13.2
LDTP07154	ATPase family AAA domain-containing protein 3B (ATAD3B)					13.0
LDTP12954	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 (HACD3)					12.4
LDTP12608	Phosphatidylinositol-3-phosphatase SAC1 (SACM1L)					12.4
LDTP03689	Serine hydroxymethyltransferase, mitochondrial (SHMT2)					12.3
LDTP02602	Histidine--tRNA ligase, cytoplasmic (HARS1)					12.2
LDTP04251	Elongation factor Tu, mitochondrial (TUFM)					12.0
LDTP12588	Isoleucine--tRNA ligase, mitochondrial (IARS2)					12.0
LDTP10318	Ubiquitin thioesterase OTUB1 (OTUB1)					11.9
LDTP02848	NADPH--cytochrome P450 reductase (POR)					11.7
LDTP12681	ATPase family AAA domain-containing protein 3A (ATAD3A)					10.9
LDTP01561	NADH dehydrogenase 1 alpha subcomplex subunit 10, mitochondrial (NDUFA10)					10.7
LDTP03417	Proteasome subunit beta type-4 (PSMB4)					10.5
LDTP03912	Trifunctional enzyme subunit alpha, mitochondrial (HADHA)					10.1
LDTP04120	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (STT3A)					9.6
LDTP02375	Poly [ADP-ribose] polymerase 1 (PARP1)					9.0
LDTP05128	Glutathione S-transferase omega-1 (GSTO1)					8.9
LDTP05862	NAD(P) transhydrogenase, mitochondrial (NNT)					7.9
LDTP03610	Cytochrome b-c1 complex subunit 1, mitochondrial (UQCRC1)					7.8
LDTP01770	NADH-cytochrome b5 reductase 3 (CYB5R3)					7.6
LDTP04399	Ras-related protein Rab-7a (RAB7A)					7.3
LDTP11874	Thioredoxin-related transmembrane protein 1 (TMX1)					7.2
LDTP03770	Alpha-adducin (ADD1)					6.7
LDTP06200	Delta(14)-sterol reductase LBR (LBR)					6.6
LDTP12628	Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10)					6.2
LDTP02640	X-ray repair cross-complementing protein 6 (XRCC6)					6.1
LDTP13816	RuvB-like 1 (RUVBL1)					5.9
LDTP03680	DNA replication licensing factor MCM4 (MCM4)					5.8
LDTP02150	ATP synthase subunit beta, mitochondrial (ATP5F1B)					5.7
LDTP04144	Cytochrome b-c1 complex subunit Rieske, mitochondrial (UQCRFS1)					5.6
LDTP00246	Eukaryotic translation initiation factor 3 subunit F (EIF3F)					5.6
LDTP03424	ATP-binding cassette sub-family D member 3 (ABCD3)					5.5
LDTP02922	CTP synthase 1 (CTPS1)					5.5
LDTP03813	Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial (CPOX)					5.4
LDTP04561	ATP-citrate synthase (ACLY)					5.1
LDTP00314	ATP-dependent RNA helicase DDX3X (DDX3X)					5.0

Target ID	Target Name					Binding Ratio
LDTP03284	ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB)					20.0
LDTP10036	BOS complex subunit NCLN (NCLN)					20.0
LDTP05511	Cytoskeleton-associated protein 4 (CKAP4)					20.0
LDTP01301	Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial (SLC25A12)					20.0
LDTP13393	Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial (SLC25A13)					20.0
LDTP03277	ER lumen protein-retaining receptor 1 (KDELR1)					20.0
LDTP06034	Lysosome membrane protein 2 (SCARB2)					20.0
LDTP05900	Metaxin-1 (MTX1)					20.0
LDTP00865	Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20)					20.0
LDTP10913	Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A)					20.0
LDTP01059	Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B)					20.0
LDTP14952	Mpv17-like protein 2 (MPV17L2)					20.0
LDTP00541	NPC intracellular cholesterol transporter 1 (NPC1)					20.0
LDTP11213	Nucleoporin NDC1 (NDC1)					20.0
LDTP10059	PAT complex subunit CCDC47 (CCDC47)					20.0
LDTP02215	Prosaposin (PSAP)					20.0
LDTP01745	Putative lipid scramblase CLPTM1 (CLPTM1)					20.0
LDTP09547	Scavenger receptor class B member 1 (SCARB1)					20.0
LDTP06945	Sigma intracellular receptor 2 (TMEM97)					20.0
LDTP17641	Solute carrier family 35 member F2 (SLC35F2)					20.0
LDTP06426	Translocating chain-associated membrane protein 1 (TRAM1)					20.0
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					20.0
LDTP04071	Voltage-dependent anion-selective channel protein 2 (VDAC2)					20.0
LDTP13174	Mitochondrial dicarboxylate carrier (SLC25A10)					19.2
LDTP04033	Translocon-associated protein subunit alpha (SSR1)					18.9
LDTP06660	MICOS complex subunit MIC60 (IMMT)					18.3
LDTP00562	Membrane-associated progesterone receptor component 2 (PGRMC2)					17.5
LDTP04707	Protein SEC13 homolog (SEC13)					17.4
LDTP14084	Sorting and assembly machinery component 50 homolog (SAMM50)					16.5
LDTP14235	Mitochondrial carrier homolog 2 (MTCH2)					15.9
LDTP02057	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (RPN1)					15.3
LDTP00592	Surfeit locus protein 4 (SURF4)					14.6
LDTP03405	Calnexin (CANX)					13.5
LDTP13824	Voltage-dependent anion-selective channel protein 3 (VDAC3)					12.3
LDTP02058	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (RPN2)					11.6
LDTP01525	Mitochondrial proton/calcium exchanger protein (LETM1)					11.6
LDTP01969	Transferrin receptor protein 1 (TFRC)					11.0
LDTP03559	High affinity cationic amino acid transporter 1 (SLC7A1)					10.6
LDTP10081	Leucine-rich repeat-containing protein 59 (LRRC59)					9.3
LDTP08769	Nuclear pore complex protein Nup93 (NUP93)					7.5
LDTP01748	Mitochondrial import receptor subunit TOM40 homolog (TOMM40)					7.4
LDTP00235	Membrane-associated progesterone receptor component 1 (PGRMC1)					6.6
LDTP00451	Secretory carrier-associated membrane protein 3 (SCAMP3)					6.4
LDTP00821	Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44)					6.1
LDTP05238	Solute carrier family 25 member 3 (SLC25A3)					6.0
LDTP02608	ADP/ATP translocase 1 (SLC25A4)					5.9
LDTP04501	Importin subunit alpha-1 (KPNA2)					5.9
LDTP09949	Transportin-1 (TNPO1)					5.9
LDTP02609	ADP/ATP translocase 3 (SLC25A6)					5.7
LDTP02087	ADP/ATP translocase 2 (SLC25A5)					5.5
LDTP05384	Mitochondrial 2-oxoglutarate/malate carrier protein (SLC25A11)					5.1
LDTP13416	Stomatin-like protein 2, mitochondrial (STOML2)					5.1
LDTP03327	ATP synthase subunit alpha, mitochondrial (ATP5F1A)					5.0

Target ID	Target Name					Binding Ratio
LDTP10891	DnaJ homolog subfamily C member 2 (DNAJC2)					20.0
LDTP05065	Y-box-binding protein 1 (YBX1)					12.7
LDTP03212	Splicing factor, proline- and glutamine-rich (SFPQ)					6.8

Target ID	Target Name					Binding Ratio
LDTP03772	Basigin (BSG)					15.1

Target ID	Target Name					Binding Ratio
LDTP08364	Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2)					20.0
LDTP03030	Annexin A7 (ANXA7)					20.0
LDTP13755	Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2)					20.0
LDTP00887	Calumenin (CALU)					20.0
LDTP01652	Centrosomal protein 43 (CEP43)					20.0
LDTP04518	DNA mismatch repair protein Msh6 (MSH6)					20.0
LDTP05922	Dynactin subunit 2 (DCTN2)					20.0
LDTP03678	ER lumen protein-retaining receptor 2 (KDELR2)					20.0
LDTP09920	Golgi apparatus protein 1 (GLG1)					20.0
LDTP00281	Golgi integral membrane protein 4 (GOLIM4)					20.0
LDTP08425	Hornerin (HRNR)					20.0
LDTP02756	Junction plakoglobin (JUP)					20.0
LDTP05400	Lamin-B2 (LMNB2)					20.0
LDTP04094	Large proline-rich protein BAG6 (BAG6)					20.0
LDTP16192	Mitochondrial fission process protein 1 (MTFP1)					20.0
LDTP12565	Mitochondrial import receptor subunit TOM22 homolog (TOMM22)					20.0
LDTP08594	Negative elongation factor C/D (NELFCD)					20.0
LDTP13630	Neudesin (NENF)					20.0
LDTP05375	Nucleobindin-1 (NUCB1)					20.0
LDTP05160	Nucleobindin-2 (NUCB2)					20.0
LDTP10056	Protein FAM162A (FAM162A)					20.0
LDTP08224	Protein LYRIC (MTDH)					20.0
LDTP00105	Protein unc-119 homolog B (UNC119B)					20.0
LDTP06097	Reticulocalbin-2 (RCN2)					20.0
LDTP12412	Reticulon-4 (RTN4)					20.0
LDTP19372	Tetratricopeptide repeat protein 38 (TTC38)					20.0
LDTP03065	Lamin-B1 (LMNB1)					19.3
LDTP10965	Nucleosome assembly protein 1-like 4 (NAP1L4)					18.5
LDTP16323	Signal recognition particle receptor subunit beta (SRPRB)					18.2
LDTP14486	Clustered mitochondria protein homolog (CLUH)					18.2
LDTP04684	Nucleosome assembly protein 1-like 1 (NAP1L1)					17.9
LDTP11239	Protein misato homolog 1 (MSTO1)					16.0
LDTP02297	Vimentin (VIM)					14.7
LDTP08707	Proline-, glutamic acid- and leucine-rich protein 1 (PELP1)					14.2
LDTP04652	Eukaryotic translation initiation factor 5 (EIF5)					13.9
LDTP03968	Lamina-associated polypeptide 2, isoforms beta/gamma (TMPO)					13.8
LDTP08760	Cell cycle and apoptosis regulator protein 2 (CCAR2)					13.3
LDTP03614	Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3)					13.2
LDTP13167	Peflin (PEF1)					13.1
LDTP00500	Heterogeneous nuclear ribonucleoprotein D-like (HNRNPDL)					13.0
LDTP11233	Tubulin beta-6 chain (TUBB6)					13.0
LDTP08058	Mitochondrial antiviral-signaling protein (MAVS)					12.9
LDTP15705	BTB/POZ domain-containing protein KCTD12 (KCTD12)					12.8
LDTP05484	Proteasome activator complex subunit 1 (PSME1)					12.8
LDTP05076	Tubulin beta-4B chain (TUBB4B)					12.4
LDTP03775	RNA-binding protein FUS (FUS)					12.2
LDTP11277	Tubulin beta-2B chain (TUBB2B)					12.2
LDTP02205	Tubulin beta chain (TUBB)					12.0
LDTP00991	Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP)					12.0
LDTP05904	Tubulin beta-3 chain (TUBB3)					11.9
LDTP04714	Heterogeneous nuclear ribonucleoprotein H2 (HNRNPH2)					11.7
LDTP05525	KH domain-containing, RNA-binding, signal transduction-associated protein 1 (KHDRBS1)					11.7
LDTP00756	Heterogeneous nuclear ribonucleoprotein R (HNRNPR)					11.6
LDTP10684	RNA-binding protein 14 (RBM14)					11.1
LDTP03967	Lamina-associated polypeptide 2, isoform alpha (TMPO)					11.0
LDTP10078	Far upstream element-binding protein 1 (FUBP1)					11.0
LDTP10962	Heterogeneous nuclear ribonucleoprotein A/B (HNRNPAB)					10.4
LDTP17065	Heterogeneous nuclear ribonucleoprotein U-like protein 2 (HNRNPUL2)					10.4
LDTP09881	TATA-binding protein-associated factor 2N (TAF15)					9.8
LDTP04514	Heterogeneous nuclear ribonucleoprotein F (HNRNPF)					9.4
LDTP02631	Alpha-actinin-1 (ACTN1)					9.2
LDTP03615	Heterogeneous nuclear ribonucleoprotein H (HNRNPH1)					8.8
LDTP03182	Heterogeneous nuclear ribonucleoproteins A2/B1 (HNRNPA2B1)					8.0
LDTP06032	Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD)					8.0
LDTP09938	Far upstream element-binding protein 2 (KHSRP)					7.6
LDTP04495	Heterogeneous nuclear ribonucleoprotein A3 (HNRNPA3)					7.1
LDTP01932	Prelamin-A/C (LMNA)					6.4
LDTP05277	Protein SET (SET)					6.0
LDTP04952	Heterogeneous nuclear ribonucleoprotein K (HNRNPK)					5.9
LDTP00398	Insulin receptor substrate 4 (IRS4)					5.6
LDTP03850	RNA-binding motif protein, X chromosome (RBMX)					5.6
LDTP11324	RNA-binding protein 4 (RBM4)					5.6
LDTP02227	Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC)					5.4
LDTP04356	Emerin (EMD)					5.3
LDTP10408	Far upstream element-binding protein 3 (FUBP3)					5.3
LDTP04027	Matrin-3 (MATR3)					5.0
LDTP04249	T-complex protein 1 subunit gamma (CCT3)					5.0

1	Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.