Details of the Target

General Information of Target

Target ID LDTP03770
Target Name Alpha-adducin (ADD1)
Gene Name ADD1
Gene ID 118
Synonyms
ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSM
ILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMS
LGMVTPVNDLRGSDSIAYDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHF
LIVPFGLLYSEVTASSLVKINLQGDIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCVVHI
HTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILR
NHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYKAKSRSPGSPV
GEGTGSPPKWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYS
FASDGDSGTCSPLRHSFQKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTKWTKEDGHRT
STSAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVT
ASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYRREVERKQKGSEENLDEAREQKEKS
PPDQPAVPHPPPSTPIKLEEDLVPEPTTGDDSDAATFKPTLPDLSPDEPSEALGFPMLEK
EEEAHRPPSPTEAPTEASPEPAPDPAPVAEEAAPSAVEEGAAADPGSDGSPGKSPSKKKK
KFRTPSFLKKSKKKSDS
Target Bioclass
Enzyme
Family
Aldolase class II family, Adducin subfamily
Subcellular location
Cytoplasm, cytoskeleton
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Uniprot ID
P35611
Ensemble ID
ENST00000264758.11
HGNC ID
HGNC:243

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 30 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
STPyne
 Probe Info  		K439(7.03); K474(9.09); K55(5.70); K729(1.80)  LDD0277  [2]
Probe 1
 Probe Info  		Y35(5.99); Y575(24.67)  LDD3495  [3]
DBIA
 Probe Info  		C236(2.08); C556(1.33)  LDD3310  [4]
AHL-Pu-1
 Probe Info  		C68(2.80)  LDD0168  [5]
EA-probe
 Probe Info  		C68(0.84)  LDD2210  [6]
HHS-475
 Probe Info  		Y419(0.95); Y35(1.06)  LDD0264  [7]
5E-2FA
 Probe Info  		N.A.  LDD2235  [8]
ATP probe
 Probe Info  		K21(0.00); K439(0.00)  LDD0199  [9]
4-Iodoacetamidophenylacetylene
 Probe Info  		C253(0.00); C325(0.00); C525(0.00); C68(0.00)  LDD0038  [10]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [11]
IPIAA_H
 Probe Info  		C253(0.00); C68(0.00)  LDD0030  [12]
IPIAA_L
 Probe Info  		C253(0.00); C68(0.00); C525(0.00)  LDD0031  [12]
Lodoacetamide azide
 Probe Info  		C253(0.00); C325(0.00); C525(0.00); C68(0.00)  LDD0037  [10]
NAIA_4
 Probe Info  		C68(0.00); C430(0.00); C525(0.00)  LDD2226  [13]
TFBX
 Probe Info  		C525(0.00); C430(0.00)  LDD0027  [14]
DA-P3
 Probe Info  		N.A.  LDD0178  [15]
Compound 10
 Probe Info  		N.A.  LDD2216  [16]
Compound 11
 Probe Info  		N.A.  LDD2213  [16]
IPM
 Probe Info  		N.A.  LDD0005  [17]
NHS
 Probe Info  		N.A.  LDD0010  [17]
VSF
 Probe Info  		C525(0.00); C430(0.00)  LDD0007  [17]
Phosphinate-6
 Probe Info  		C430(0.00); C525(0.00)  LDD0018  [18]
1c-yne
 Probe Info  		K548(0.00); K369(0.00)  LDD0228  [19]
1d-yne
 Probe Info  		N.A.  LDD0357  [19]
Acrolein
 Probe Info  		N.A.  LDD0217  [20]
W1
 Probe Info  		N.A.  LDD0236  [21]
AOyne
 Probe Info  		14.90  LDD0443  [22]
NAIA_5
 Probe Info  		C253(0.00); C68(0.00)  LDD2223  [13]
HHS-465
 Probe Info  		N.A.  LDD2240  [23]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		5.56  LDD0471  [24]
FFF probe12
 Probe Info  		17.75  LDD0473  [24]
FFF probe13
 Probe Info  		20.00  LDD0475  [24]
FFF probe14
 Probe Info  		20.00  LDD0477  [24]
FFF probe2
 Probe Info  		17.04  LDD0463  [24]
FFF probe3
 Probe Info  		20.00  LDD0464  [24]
FFF probe6
 Probe Info  		20.00  LDD0482  [24]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C68(2.80)  LDD0168  [5]
 LDCM0026  4SU-RNA+native RNA HEK-293T C68(6.66); C430(2.15)  LDD0169  [5]
 LDCM0156  Aniline NCI-H1299 C430(0.00); C525(0.00)  LDD0404  [1]
 LDCM0630  CCW28-3 231MFP C430(1.85); C68(1.30)  LDD2214  [25]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [20]
 LDCM0175  Ethacrynic acid HeLa C68(0.84)  LDD2210  [6]
 LDCM0625  F8 Ramos C525(1.13); C430(0.56)  LDD2187  [26]
 LDCM0572  Fragment10 MDA-MB-231 C68(1.94)  LDD1465  [27]
 LDCM0573  Fragment11 MDA-MB-231 C68(0.91)  LDD1467  [27]
 LDCM0574  Fragment12 MDA-MB-231 C68(3.58)  LDD1468  [27]
 LDCM0575  Fragment13 MDA-MB-231 C68(1.05)  LDD1469  [27]
 LDCM0576  Fragment14 MDA-MB-231 C68(1.60)  LDD1471  [27]
 LDCM0579  Fragment20 Ramos C525(1.13); C430(1.75)  LDD2194  [26]
 LDCM0580  Fragment21 MDA-MB-231 C68(0.78)  LDD1473  [27]
 LDCM0582  Fragment23 Ramos C525(1.30); C430(1.33)  LDD2196  [26]
 LDCM0578  Fragment27 MDA-MB-231 C68(0.85)  LDD1474  [27]
 LDCM0586  Fragment28 MDA-MB-231 C68(1.71)  LDD1475  [27]
 LDCM0588  Fragment30 Ramos C525(1.08); C430(1.15)  LDD2199  [26]
 LDCM0589  Fragment31 Ramos C525(1.49); C430(0.95)  LDD2200  [26]
 LDCM0590  Fragment32 Ramos C525(1.97)  LDD2201  [26]
 LDCM0468  Fragment33 MDA-MB-231 C68(1.59)  LDD1479  [27]
 LDCM0596  Fragment38 MDA-MB-231 C68(1.35)  LDD1480  [27]
 LDCM0566  Fragment4 MDA-MB-231 C68(4.30)  LDD1461  [27]
 LDCM0599  Fragment41 MDA-MB-231 C68(0.88)  LDD1481  [27]
 LDCM0603  Fragment45 MDA-MB-231 C68(20.00)  LDD1482  [27]
 LDCM0427  Fragment51 MDA-MB-231 C68(1.04)  LDD1483  [27]
 LDCM0610  Fragment52 Ramos C525(0.98); C430(0.61)  LDD2204  [26]
 LDCM0614  Fragment56 MDA-MB-231 C68(0.77)  LDD1484  [27]
 LDCM0569  Fragment7 Ramos C525(1.27); C430(1.24)  LDD2186  [26]
 LDCM0570  Fragment8 MDA-MB-231 C68(2.42)  LDD1462  [27]
 LDCM0571  Fragment9 MDA-MB-231 C68(4.34)  LDD1463  [27]
 LDCM0116  HHS-0101 DM93 Y419(0.95); Y35(1.06)  LDD0264  [7]
 LDCM0117  HHS-0201 DM93 Y35(0.59); Y419(0.82)  LDD0265  [7]
 LDCM0118  HHS-0301 DM93 Y35(0.83); Y419(0.88)  LDD0266  [7]
 LDCM0119  HHS-0401 DM93 Y35(0.69); Y419(1.85)  LDD0267  [7]
 LDCM0120  HHS-0701 DM93 Y35(1.36)  LDD0268  [7]
 LDCM0022  KB02 MDA-MB-231 C68(5.75)  LDD1460  [27]
 LDCM0023  KB03 HEK-293T C236(0.95); C525(1.07); C430(1.05); C253(1.04)  LDD1497  [28]
 LDCM0024  KB05 COLO792 C236(2.08); C556(1.33)  LDD3310  [4]
 LDCM0627  NUDT7-COV-1 HEK-293T C525(0.88); C430(0.78)  LDD2206  [29]
 LDCM0628  OTUB2-COV-1 HEK-293T C525(0.80); C430(0.44)  LDD2207  [29]
 LDCM0131  RA190 MM1.R C430(1.75); C525(1.51)  LDD0304  [30]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Serine/threonine-protein kinase PAK 1 (PAK1) STE Ser/Thr protein kinase family Q13153
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-crystallin A chain (CRYAA) Small heat shock protein (HSP20) family P02489
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
High mobility group protein 20A (HMG20A) . Q9NP66

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
6 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
7 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
8 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
9 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
10 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
11 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
12 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
13 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
14 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
15 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
16 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
17 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
18 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
19 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
20 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
21 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
22 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
23 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
24 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
25 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
26 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
27 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
28 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
29 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
30 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.