Details of the Probe

General Information of Probe

Probe ID
LDPC0001
Probe Name
aHNE
Synonyms
1011268-23-7; (E)-4-Hydroxynon-2-en-8-ynal; 4-hydroxy Nonenal Alkyne; 4-Hydroxynonenal alkyne; (2E)-4-HYDROXYNON-2-EN-8-YNAL; Alkynyl 4-hydroxy-2-nonenal; AKOS040754962; BP-22691; PD021450; HY-113803; CS-0062904
Probe Type
ABPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 152.19
Lipid-water partition coefficient (xlogp) 0.6
Rotatable Bond Count (rotbonds) 5
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 2
Chemical Identifiers
Formula
C9H12O2
IUPAC Name
(E)-4-hydroxynon-2-en-8-ynal
Canonical SMILES
C#CCCCC(C=CC=O)O
InChI
InChI=1S/C9H12O2/c1-2-3-4-6-9(11)7-5-8-10/h1,5,7-9,11H,3-4,6H2/b7-5+
InChIKey
RXECVCDYORIBBT-FNORWQNLSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

65 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
2',5'-phosphodiesterase 12 (PDE12) LDTP07447 C119  LDD0001 
26S proteasome non-ATPase regulatory subunit 2 (PSMD2) LDTP05782 C459  LDD0001 
5'-nucleotidase domain-containing protein 1 (NT5DC1) LDTP17247 C119  LDD0001 
Actin, cytoplasmic 1 (ACTB) LDTP04876 C272  LDD0001 
Activating signal cointegrator 1 complex subunit 3 (ASCC3) LDTP08826 C1315  LDD0001 
Adenosylhomocysteinase (AHCY) LDTP03242 C421  LDD0001 
Adenylate kinase 2, mitochondrial (AK2) LDTP04636 C40  LDD0001 
ADP-ribosylation factor-related protein 1 (ARFRP1) LDTP05982 C125  LDD0001 
Amidophosphoribosyltransferase (PPAT) LDTP05480 C100  LDD0001 
Aspartyl aminopeptidase (DNPEP) LDTP13527 C337  LDD0001 
ATP-dependent DNA helicase Q1 (RECQL) LDTP04083 C606  LDD0001 
Baculoviral IAP repeat-containing protein 6 (BIRC6) LDTP12459 C463  LDD0001 
Bifunctional glutamate/proline--tRNA ligase (EPRS1) LDTP02222 C92; C744  LDD0001 
Calpain-2 catalytic subunit (CAPN2) LDTP02911 C82  LDD0001 
Catechol O-methyltransferase (COMT) LDTP03145 C223  LDD0001 
Cytochrome P450 3A43 (CYP3A43) LDTP12159 C152  LDD0001 
D-3-phosphoglycerate dehydrogenase (PHGDH) LDTP00701 C369  LDD0001 
DNA dC->dU-editing enzyme APOBEC-3C (APOBEC3C) LDTP12539 C130  LDD0001 
DNA-dependent protein kinase catalytic subunit (PRKDC) LDTP05136 C1904; C2342  LDD0001 
Dolichol-phosphate mannosyltransferase subunit 3 (DPM3) LDTP13087 C67  LDD0001 
Double-stranded RNA-specific adenosine deaminase (ADAR) LDTP04690 C622  LDD0001 
Dual specificity mitogen-activated protein kinase kinase 3 (MAP2K3) LDTP04103 C29  LDD0001 
Dynamin-1-like protein (DNM1L) LDTP00273 C505  LDD0001 
Dynamin-like 120 kDa protein, mitochondrial (OPA1) LDTP00950 C801  LDD0001 
E3 ubiquitin-protein ligase MYCBP2 (MYCBP2) LDTP01265 C1169  LDD0001 
E3 ubiquitin-protein ligase SMURF2 (SMURF2) LDTP12138 C47  LDD0001 
Elongation factor 2 (EEF2) LDTP02672 C41; C728  LDD0001 
Elongation factor Tu, mitochondrial (TUFM) LDTP04251 C130  LDD0001 
Exosome complex component 10 (EXOSC10) LDTP05312 C612  LDD0001 
Fatty acid synthase (FASN) LDTP04243 C1448  LDD0001 
Glutamate--cysteine ligase regulatory subunit (GCLM) LDTP04186 C35  LDD0001 
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) LDTP02038 C152  LDD0001 
Heat shock protein HSP 90-alpha (HSP90AA1) LDTP02225 C420  LDD0001 
Heme oxygenase 2 (HMOX2) LDTP03540 C265  LDD0001 
Histone-lysine N-methyltransferase SMYD3 (SMYD3) LDTP12020 C333  LDD0001 
Myosin-9 (MYH9) LDTP03764 C91; C1379  LDD0001 
N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) LDTP11273 C195  LDD0001 
Peroxiredoxin-1 (PRDX1) LDTP05503 C173  LDD0001 
Peroxiredoxin-4 (PRDX4) LDTP05773 C51  LDD0001 
Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) LDTP06307 C199  LDD0001 
Phosphatidylserine synthase 1 (PTDSS1) LDTP04203 C419  LDD0001 
Pre-rRNA-processing protein TSR1 homolog (TSR1) LDTP14888 C126  LDD0001 
Probable ubiquitin carboxyl-terminal hydrolase FAF-X (USP9X) LDTP09961 C842  LDD0001 
Prostaglandin E synthase 3 (PTGES3) LDTP06338 C58  LDD0001 
Protein argonaute-2 (AGO2) LDTP13492 C188  LDD0001 
Pseudouridylate synthase RPUSD2 (RPUSD2) LDTP15370 C246  LDD0001 
RAF proto-oncogene serine/threonine-protein kinase (RAF1) LDTP02002 C637  LDD0001 
Ras-related C3 botulinum toxin substrate 1 (RAC1) LDTP05037 C105  LDD0001 
Ras-related protein Rab-14 (RAB14) LDTP04901 C40  LDD0001 
Replication factor C subunit 3 (RFC3) LDTP03911 C193  LDD0001 
Ribonucleoside-diphosphate reductase large subunit (RRM1) LDTP03259 C492  LDD0001 
Ribosomal RNA-processing protein 8 (RRP8) LDTP00693 C451  LDD0001 
Sialic acid synthase (NANS) LDTP12473 C287  LDD0001 
Signal peptidase complex subunit 2 (SPCS2) LDTP06270 C26; C17  LDD0001 
Thioredoxin (TXN) LDTP02502 C73  LDD0001 
TNF receptor-associated factor 2 (TRAF2) LDTP05698 C287  LDD0001 
Tubulin alpha-1B chain (TUBA1B) LDTP05074 C347; C295  LDD0001 
Tubulin alpha-1C chain (TUBA1C) LDTP11074 C347  LDD0001 
Tyrosine-protein kinase BAZ1B (BAZ1B) LDTP13344 C156  LDD0001 
Ubiquitin carboxyl-terminal hydrolase 15 (USP15) LDTP14051 C634  LDD0001 
Ubiquitin carboxyl-terminal hydrolase 7 (USP7) LDTP09962 C961  LDD0001 
Ubiquitin-conjugating enzyme E2 S (UBE2S) LDTP06621 C118  LDD0001 
Ubiquitin-like modifier-activating enzyme 6 (UBA6) LDTP00013 C347  LDD0001 
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (OGT) LDTP00601 C323  LDD0001 
Uridine phosphorylase 1 (UPP1) LDTP06643 C162  LDD0001 
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⏷ Show the Full List of 65 Enzyme
22 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
14-3-3 protein epsilon (YWHAE) LDTP04969 C98  LDD0001 
14-3-3 protein theta (YWHAQ) LDTP03385 C134  LDD0001 
Apoptotic chromatin condensation inducer in the nucleus (ACIN1) LDTP13490 C1083  LDD0001 
Catenin delta-1 (CTNND1) LDTP01037 C618  LDD0001 
Clathrin heavy chain 1 (CLTC) LDTP05252 C870  LDD0001 
Cystatin-B (CSTB) LDTP02009 C3  LDD0001 
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (RPN1) LDTP02057 C477  LDD0001 
Eukaryotic translation initiation factor 5A-2 (EIF5A2) LDTP11657 C73  LDD0001 
Exportin-T (XPOT) LDTP00810 C650  LDD0001 
Extended synaptotagmin-1 (ESYT1) LDTP11161 C635  LDD0001 
Fatty acid-binding protein 5 (FABP5) LDTP05292 C120  LDD0001 
Filamin-A (FLNA) LDTP03107 C2543  LDD0001 
Importin-4 (IPO4) LDTP09515 C350  LDD0001 
Importin-5 (IPO5) LDTP00266 C687  LDD0001 
Mitochondrial import receptor subunit TOM40 homolog (TOMM40) LDTP01748 C74  LDD0001 
Nuclear pore complex protein Nup93 (NUP93) LDTP08769 C522  LDD0001 
Nucleoporin NUP35 (NUP35) LDTP09204 C255  LDD0001 
Phosphorylated adapter RNA export protein (PHAX) LDTP12043 C51  LDD0001 
Signal recognition particle subunit SRP68 (SRP68) LDTP13262 C525  LDD0001 
Spectrin beta chain, non-erythrocytic 1 (SPTBN1) LDTP05273 C604  LDD0001 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 C76  LDD0001 
Voltage-dependent anion-selective channel protein 3 (VDAC3) LDTP13824 C65  LDD0001 
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⏷ Show the Full List of 22 Transporter and channel
4 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Fos-related antigen 1 (FOSL1) LDTP02786 C97  LDD0001 
Transcription factor p65 (RELA) LDTP05417 C109  LDD0001 
Transcriptional repressor CTCF (CTCF) LDTP04281 C155  LDD0001 
Zinc finger protein 512 (ZNF512) LDTP18206 C324  LDD0001 
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73 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
182 kDa tankyrase-1-binding protein (TNKS1BP1) LDTP11597 C1443  LDD0001 
Acyl carrier protein, mitochondrial (NDUFAB1) LDTP00372 C140  LDD0001 
Alpha-actinin-1 (ACTN1) LDTP02631 C370  LDD0001 
Ataxin-2 (ATXN2) LDTP10947 C892  LDD0001 
BRCA1-associated ATM activator 1 (BRAT1) LDTP07598 C673  LDD0001 
Bromodomain adjacent to zinc finger domain protein 2A (BAZ2A) LDTP13343 C917  LDD0001 
C2 domain-containing protein 2 (C2CD2) LDTP18575 C244  LDD0001 
Calponin-2 (CNN2) LDTP10864 C175; C215  LDD0001 
Calponin-3 (CNN3) LDTP06383 C173  LDD0001 
CDGSH iron-sulfur domain-containing protein 2 (CISD2) LDTP08902 C92  LDD0001 
Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) LDTP06584 C159  LDD0001 
Coatomer subunit beta (COPB1) LDTP04570 C248  LDD0001 
Cofilin-1 (CFL1) LDTP03244 C139  LDD0001 
Complex III assembly factor LYRM7 (LYRM7) LDTP07200 C97  LDD0001 
Constitutive coactivator of PPAR-gamma-like protein 1 (FAM120A) LDTP12883 C919; C531; C1088; C1103  LDD0001 
Coronin-1C (CORO1C) LDTP13580 C456  LDD0001 
Cytoskeleton-associated protein 5 (CKAP5) LDTP06021 C1113  LDD0001 
Destrin (DSTN) LDTP04887 C135  LDD0001 
Elongation factor 1-gamma (EEF1G) LDTP03367 C266  LDD0001 
Elongator complex protein 2 (ELP2) LDTP07400 C204  LDD0001 
Eukaryotic translation initiation factor 4B (EIF4B) LDTP03247 C457  LDD0001 
F-actin-capping protein subunit beta (CAPZB) LDTP04127 C206  LDD0001 
H/ACA ribonucleoprotein complex subunit 1 (GAR1) LDTP12796 C80  LDD0001 
Heat shock 70 kDa protein 4L (HSPA4L) LDTP14545 C540  LDD0001 
Heterogeneous nuclear ribonucleoprotein F (HNRNPF) LDTP04514 C267  LDD0001 
Heterogeneous nuclear ribonucleoprotein H (HNRNPH1) LDTP03615 C267  LDD0001 
Heterogeneous nuclear ribonucleoprotein K (HNRNPK) LDTP04952 C132  LDD0001 
Heterogeneous nuclear ribonucleoprotein L (HNRNPL) LDTP02750 C472  LDD0001 
Heterogeneous nuclear ribonucleoprotein U (HNRNPU) LDTP05259 C594  LDD0001 
Histone-binding protein RBBP7 (RBBP7) LDTP06562 C97  LDD0001 
Large ribosomal subunit protein eL18 (RPL18) LDTP05509 C134  LDD0001 
LIM domain-containing protein 1 (LIMD1) LDTP13241 C431  LDD0001 
Matrin-3 (MATR3) LDTP04027 C230  LDD0001 
Mediator of DNA damage checkpoint protein 1 (MDC1) LDTP06176 C1742  LDD0001 
Mini-chromosome maintenance complex-binding protein (MCMBP) LDTP11197 C325  LDD0001 
Myb-binding protein 1A (MYBBP1A) LDTP11079 C942  LDD0001 
NGFI-A-binding protein 2 (NAB2) LDTP06456 C481  LDD0001 
Nuclear autoantigenic sperm protein (NASP) LDTP04241 C708  LDD0001 
Nucleolar complex protein 2 homolog (NOC2L) LDTP14011 C585  LDD0001 
Nucleolar protein 6 (NOL6) LDTP11993 C265  LDD0001 
Nucleolar protein 9 (NOP9) LDTP15283 C242  LDD0001 
Otogelin-like protein (OTOGL) LDTP14920 C1136  LDD0001 
Perilipin-3 (PLIN3) LDTP01021 C39  LDD0001 
Plastin-2 (LCP1) LDTP02691 C101  LDD0001 
Poly(rC)-binding protein 1 (PCBP1) LDTP06366 C201; C194  LDD0001 
POTE ankyrin domain family member F (POTEF) LDTP14342 C965  LDD0001 
Proliferation marker protein Ki-67 (MKI67) LDTP04078 C643  LDD0001 
Proteasome activator complex subunit 1 (PSME1) LDTP05484 C22  LDD0001 
Protein SCAF11 (SCAF11) LDTP10909 C542  LDD0001 
Protein transport protein Sec24C (SEC24C) LDTP04594 C1083  LDD0001 
Putative RNA-binding protein Luc7-like 2 (LUC7L2) LDTP13956 C348  LDD0001 
Ribonuclease inhibitor (RNH1) LDTP02656 C75  LDD0001 
RRP12-like protein (RRP12) LDTP14980 C31  LDD0001 
Scaffold attachment factor B1 (SAFB) LDTP06385 C362  LDD0001 
Serine-threonine kinase receptor-associated protein (STRAP) LDTP13991 C305  LDD0001 
Serine/threonine-protein phosphatase 4 regulatory subunit 2 (PPP4R2) LDTP12800 C22  LDD0001 
Shootin-1 (SHTN1) LDTP00019 C565  LDD0001 
Small subunit processome component 20 homolog (UTP20) LDTP01294 C313; C2058; C312  LDD0001 
Splicing factor 3B subunit 6 (SF3B6) LDTP13970 C83  LDD0001 
Splicing factor U2AF 65 kDa subunit (U2AF2) LDTP03352 C429  LDD0001 
Stress-70 protein, mitochondrial (HSPA9) LDTP03860 C608  LDD0001 
Surfeit locus protein 6 (SURF6) LDTP14502 C189; C19  LDD0001 
Survival of motor neuron-related-splicing factor 30 (SMNDC1) LDTP01362 C214  LDD0001 
T-complex protein 1 subunit beta (CCT2) LDTP05113 C395  LDD0001 
TAR DNA-binding protein 43 (TARDBP) LDTP05767 C50  LDD0001 
Telomere-associated protein RIF1 (RIF1) LDTP07205 C2169  LDD0001 
Tropomodulin-3 (TMOD3) LDTP18494 C150  LDD0001 
Tropomyosin alpha-4 chain (TPM4) LDTP05068 C247  LDD0001 
U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) LDTP00312 C27  LDD0001 
UPF0598 protein C8orf82 (C8orf82) LDTP19499 C98  LDD0001 
WD repeat and HMG-box DNA-binding protein 1 (WDHD1) LDTP01299 C1008  LDD0001 
WD repeat-containing protein 43 (WDR43) LDTP06305 C307  LDD0001 
Zinc finger CCCH-type antiviral protein 1 (ZC3HAV1) LDTP08018 C272  LDD0001 
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⏷ Show the Full List of 73 Other

Full Information of The Labelling Profiles of This Probe

Qualitation: Labeling site identification
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
50 uM
Quantitative Method
Isotope tag
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Colon
Disease Model
Colon carcinoma [ICD-11:2B90]
Model Name
Human colon carcinoma cells (RKO)
Interaction Atlas ID
 LDD0001 
Download The Altas

References

1 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764