Details of the Target

General Information of Target

Target ID LDTP11657
Target Name Eukaryotic translation initiation factor 5A-2 (EIF5A2)
Gene Name EIF5A2
Gene ID 56648
Synonyms
Eukaryotic translation initiation factor 5A-2; eIF-5A-2; eIF-5A2; Eukaryotic initiation factor 5A isoform 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAFHVEGLIAIIVFYLLILLVGIWAAWRTKNSGSAEERSEAIIVGGRDIGLLVGGFTMTA
TWVGGGYINGTAEAVYVPGYGLAWAQAPIGYSLSLILGGLFFAKPMRSKGYVTMLDPFQQ
IYGKRMGGLLFIPALMGEMFWAAAIFSALGATISVIIDVDMHISVIISALIATLYTLVGG
LYSVAYTDVVQLFCIFVGLWISVPFALSHPAVADIGFTAVHAKYQKPWLGTVDSSEVYSW
LDSFLLLMLGGIPWQAYFQRVLSSSSATYAQVLSFLAAFGCLVMAIPAILIGAIGASTDW
NQTAYGLPDPKTTEEADMILPIVLQYLCPVYISFFGLGAVSAAVMSSADSSILSASSMFA
RNIYQLSFRQNASDKEIVWVMRITVFVFGASATAMALLTKTVYGLWYLSSDLVYIVIFPQ
LLCVLFVKGTNTYGAVAGYVSGLFLRITGGEPYLYLQPLIFYPGYYPDDNGIYNQKFPFK
TLAMVTSFLTNICISYLAKYLFESGTLPPKLDVFDAVVARHSEENMDKTILVKNENIKLD
ELALVKPRQSMTLSSTFTNKEAFLDVDSSPEGSGTEDNLQ
Target Type
Literature-reported
Target Bioclass
Transporter and channel
Family
EIF-5A family
Subcellular location
Cytoplasm
Function
Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step. Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity.
TTD ID
T13039
Uniprot ID
Q9GZV4
DrugMap ID
TTH53G9
Ensemble ID
ENST00000295822.7
HGNC ID
HGNC:3301

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
AZ-9
 Probe Info  		E42(0.86)  LDD2208  [2]
DBIA
 Probe Info  		C73(0.83); C93(1.40)  LDD3331  [3]
AHL-Pu-1
 Probe Info  		C93(3.85)  LDD0170  [4]
HHS-475
 Probe Info  		Y69(0.89)  LDD0264  [5]
HHS-465
 Probe Info  		Y69(10.00)  LDD2237  [6]
W1
 Probe Info  		C73(0.87)  LDD0237  [7]
IA-alkyne
 Probe Info  		C93(0.00); C73(0.00)  LDD0174  [8]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [9]
NAIA_5
 Probe Info  		N.A.  LDD2225  [10]
aHNE
 Probe Info  		N.A.  LDD0001  [11]
IPM
 Probe Info  		N.A.  LDD2156  [12]
SF
 Probe Info  		K27(0.00); K67(0.00); K68(0.00); K39(0.00)  LDD0028  [13]
STPyne
 Probe Info  		K68(0.00); K39(0.00); K67(0.00)  LDD0009  [11]
AOyne
 Probe Info  		15.00  LDD0443  [14]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe13
 Probe Info  		6.21  LDD0475  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C93(3.85)  LDD0170  [4]
 LDCM0226  AC11 HEK-293T C93(1.03)  LDD1509  [16]
 LDCM0230  AC113 PaTu 8988t C139(0.92); C152(0.92)  LDD1109  [17]
 LDCM0231  AC114 PaTu 8988t C139(0.60); C152(0.60)  LDD1110  [17]
 LDCM0232  AC115 PaTu 8988t C139(0.81); C152(0.81)  LDD1111  [17]
 LDCM0233  AC116 PaTu 8988t C139(0.91); C152(0.91)  LDD1112  [17]
 LDCM0234  AC117 PaTu 8988t C139(0.43); C152(0.43)  LDD1113  [17]
 LDCM0235  AC118 PaTu 8988t C139(0.94); C152(0.94)  LDD1114  [17]
 LDCM0236  AC119 PaTu 8988t C139(0.88); C152(0.88)  LDD1115  [17]
 LDCM0238  AC120 PaTu 8988t C139(1.09); C152(1.09)  LDD1117  [17]
 LDCM0239  AC121 PaTu 8988t C139(0.51); C152(0.51)  LDD1118  [17]
 LDCM0240  AC122 PaTu 8988t C139(1.08); C152(1.08)  LDD1119  [17]
 LDCM0241  AC123 PaTu 8988t C139(1.55); C152(1.55)  LDD1120  [17]
 LDCM0242  AC124 PaTu 8988t C139(1.82); C152(1.82)  LDD1121  [17]
 LDCM0243  AC125 PaTu 8988t C139(2.31); C152(2.31)  LDD1122  [17]
 LDCM0244  AC126 PaTu 8988t C139(1.11); C152(1.11)  LDD1123  [17]
 LDCM0245  AC127 PaTu 8988t C139(1.01); C152(1.01)  LDD1124  [17]
 LDCM0246  AC128 PaTu 8988t C139(0.43); C152(0.43)  LDD1125  [17]
 LDCM0247  AC129 PaTu 8988t C139(0.62); C152(0.62)  LDD1126  [17]
 LDCM0249  AC130 PaTu 8988t C139(0.55); C152(0.55)  LDD1128  [17]
 LDCM0250  AC131 PaTu 8988t C139(0.88); C152(0.88)  LDD1129  [17]
 LDCM0251  AC132 PaTu 8988t C139(0.99); C152(0.99)  LDD1130  [17]
 LDCM0252  AC133 PaTu 8988t C139(0.75); C152(0.75)  LDD1131  [17]
 LDCM0253  AC134 PaTu 8988t C139(0.67); C152(0.67)  LDD1132  [17]
 LDCM0254  AC135 PaTu 8988t C139(0.59); C152(0.59)  LDD1133  [17]
 LDCM0255  AC136 PaTu 8988t C139(0.32); C152(0.32)  LDD1134  [17]
 LDCM0256  AC137 PaTu 8988t C139(0.51); C152(0.51)  LDD1135  [17]
 LDCM0257  AC138 PaTu 8988t C139(0.45); C152(0.45)  LDD1136  [17]
 LDCM0258  AC139 PaTu 8988t C139(0.42); C152(0.42)  LDD1137  [17]
 LDCM0260  AC140 PaTu 8988t C139(0.82); C152(0.82)  LDD1139  [17]
 LDCM0261  AC141 PaTu 8988t C139(1.02); C152(1.02)  LDD1140  [17]
 LDCM0262  AC142 PaTu 8988t C139(0.74); C152(0.74)  LDD1141  [17]
 LDCM0270  AC15 HEK-293T C93(1.13)  LDD1513  [16]
 LDCM0278  AC19 HEK-293T C93(1.17)  LDD1517  [16]
 LDCM0283  AC23 HEK-293T C93(1.01)  LDD1522  [16]
 LDCM0287  AC27 HEK-293T C93(1.02)  LDD1526  [16]
 LDCM0290  AC3 HEK-293T C93(1.00)  LDD1529  [16]
 LDCM0292  AC31 HEK-293T C93(0.96)  LDD1531  [16]
 LDCM0296  AC35 HEK-293T C93(0.94)  LDD1535  [16]
 LDCM0300  AC39 HEK-293T C93(1.36)  LDD1539  [16]
 LDCM0305  AC43 HEK-293T C93(1.30)  LDD1544  [16]
 LDCM0308  AC46 PaTu 8988t C139(0.96); C152(0.96)  LDD1187  [17]
 LDCM0309  AC47 PaTu 8988t C139(0.92); C152(0.92)  LDD1188  [17]
 LDCM0310  AC48 PaTu 8988t C139(1.22); C152(1.22)  LDD1189  [17]
 LDCM0311  AC49 PaTu 8988t C139(1.35); C152(1.35)  LDD1190  [17]
 LDCM0313  AC50 PaTu 8988t C139(0.85); C152(0.85)  LDD1192  [17]
 LDCM0314  AC51 PaTu 8988t C139(0.88); C152(0.88)  LDD1193  [17]
 LDCM0315  AC52 PaTu 8988t C139(1.17); C152(1.17)  LDD1194  [17]
 LDCM0316  AC53 PaTu 8988t C139(1.68); C152(1.68)  LDD1195  [17]
 LDCM0317  AC54 PaTu 8988t C139(2.26); C152(2.26)  LDD1196  [17]
 LDCM0318  AC55 PaTu 8988t C139(1.17); C152(1.17)  LDD1197  [17]
 LDCM0319  AC56 PaTu 8988t C139(0.99); C152(0.99)  LDD1198  [17]
 LDCM0322  AC59 HEK-293T C93(1.02)  LDD1561  [16]
 LDCM0327  AC63 HEK-293T C93(0.81)  LDD1566  [16]
 LDCM0332  AC68 PaTu 8988t C139(0.86); C152(0.86)  LDD1211  [17]
 LDCM0333  AC69 PaTu 8988t C139(0.90); C152(0.90)  LDD1212  [17]
 LDCM0334  AC7 HEK-293T C93(0.87)  LDD1568  [16]
 LDCM0335  AC70 PaTu 8988t C139(0.63); C152(0.63)  LDD1214  [17]
 LDCM0336  AC71 PaTu 8988t C139(0.81); C152(0.81)  LDD1215  [17]
 LDCM0337  AC72 PaTu 8988t C139(0.84); C152(0.84)  LDD1216  [17]
 LDCM0338  AC73 PaTu 8988t C139(0.84); C152(0.84)  LDD1217  [17]
 LDCM0339  AC74 PaTu 8988t C139(0.93); C152(0.93)  LDD1218  [17]
 LDCM0340  AC75 PaTu 8988t C139(0.79); C152(0.79)  LDD1219  [17]
 LDCM0341  AC76 PaTu 8988t C139(1.57); C152(1.57)  LDD1220  [17]
 LDCM0342  AC77 PaTu 8988t C139(0.84); C152(0.84)  LDD1221  [17]
 LDCM0343  AC78 PaTu 8988t C139(1.02); C152(1.02)  LDD1222  [17]
 LDCM0344  AC79 PaTu 8988t C139(0.70); C152(0.70)  LDD1223  [17]
 LDCM0346  AC80 PaTu 8988t C139(0.65); C152(0.65)  LDD1225  [17]
 LDCM0347  AC81 PaTu 8988t C139(0.80); C152(0.80)  LDD1226  [17]
 LDCM0348  AC82 PaTu 8988t C139(1.47); C152(1.47)  LDD1227  [17]
 LDCM0156  Aniline NCI-H1299 13.85  LDD0403  [1]
 LDCM0367  CL1 PaTu 8988t C139(1.17); C152(1.17)  LDD1246  [17]
 LDCM0368  CL10 PaTu 8988t C139(1.63); C152(1.63)  LDD1247  [17]
 LDCM0370  CL101 HEK-293T C93(1.08)  LDD1574  [16]
 LDCM0371  CL102 HEK-293T C93(0.89)  LDD1575  [16]
 LDCM0374  CL105 HEK-293T C93(0.97)  LDD1578  [16]
 LDCM0375  CL106 HEK-293T C93(1.29)  LDD1579  [16]
 LDCM0378  CL109 HEK-293T C93(1.37)  LDD1582  [16]
 LDCM0379  CL11 PaTu 8988t C139(1.10); C152(1.10)  LDD1258  [17]
 LDCM0380  CL110 HEK-293T C93(1.12)  LDD1584  [16]
 LDCM0383  CL113 HEK-293T C93(1.66)  LDD1587  [16]
 LDCM0384  CL114 HEK-293T C93(1.01)  LDD1588  [16]
 LDCM0387  CL117 HEK-293T C93(1.06)  LDD1591  [16]
 LDCM0388  CL118 HEK-293T C93(1.18)  LDD1592  [16]
 LDCM0390  CL12 PaTu 8988t C139(2.20); C152(2.20)  LDD1269  [17]
 LDCM0392  CL121 PaTu 8988t C139(0.90); C152(0.90)  LDD1271  [17]
 LDCM0393  CL122 PaTu 8988t C139(1.65); C152(1.65)  LDD1272  [17]
 LDCM0394  CL123 PaTu 8988t C139(1.35); C152(1.35)  LDD1273  [17]
 LDCM0395  CL124 PaTu 8988t C139(1.58); C152(1.58)  LDD1274  [17]
 LDCM0396  CL125 HEK-293T C93(0.74)  LDD1600  [16]
 LDCM0397  CL126 HEK-293T C93(1.13)  LDD1601  [16]
 LDCM0400  CL13 PaTu 8988t C139(2.88); C152(2.88)  LDD1279  [17]
 LDCM0401  CL14 PaTu 8988t C139(7.04); C152(7.04)  LDD1280  [17]
 LDCM0402  CL15 PaTu 8988t C139(2.19); C152(2.19)  LDD1281  [17]
 LDCM0406  CL19 HEK-293T C93(1.25)  LDD1610  [16]
 LDCM0407  CL2 PaTu 8988t C139(2.07); C152(2.07)  LDD1286  [17]
 LDCM0411  CL23 HEK-293T C93(1.09)  LDD1615  [16]
 LDCM0413  CL25 HEK-293T C93(1.36)  LDD1617  [16]
 LDCM0414  CL26 HEK-293T C93(1.59)  LDD1618  [16]
 LDCM0418  CL3 PaTu 8988t C139(1.22); C152(1.22)  LDD1297  [17]
 LDCM0420  CL31 HEK-293T C93(0.95)  LDD1624  [16]
 LDCM0424  CL35 HEK-293T C93(1.93)  LDD1628  [16]
 LDCM0426  CL37 HEK-293T C93(0.44)  LDD1630  [16]
 LDCM0429  CL4 PaTu 8988t C139(3.09); C152(3.09)  LDD1308  [17]
 LDCM0433  CL43 HEK-293T C93(1.03)  LDD1637  [16]
 LDCM0437  CL47 HEK-293T C93(0.91)  LDD1641  [16]
 LDCM0439  CL49 HEK-293T C93(1.13)  LDD1643  [16]
 LDCM0440  CL5 PaTu 8988t C139(2.59); C152(2.59)  LDD1319  [17]
 LDCM0441  CL50 HEK-293T C93(1.39)  LDD1645  [16]
 LDCM0446  CL55 HEK-293T C93(1.19)  LDD1649  [16]
 LDCM0450  CL59 HEK-293T C93(1.28)  LDD1653  [16]
 LDCM0451  CL6 PaTu 8988t C139(3.01); C152(3.01)  LDD1330  [17]
 LDCM0453  CL61 HEK-293T C93(1.44)  LDD1656  [16]
 LDCM0454  CL62 HEK-293T C93(0.98)  LDD1657  [16]
 LDCM0459  CL67 HEK-293T C93(1.25)  LDD1662  [16]
 LDCM0462  CL7 PaTu 8988t C139(1.63); C152(1.63)  LDD1341  [17]
 LDCM0464  CL71 HEK-293T C93(0.88)  LDD1667  [16]
 LDCM0466  CL73 HEK-293T C93(1.10)  LDD1669  [16]
 LDCM0467  CL74 HEK-293T C93(1.36)  LDD1670  [16]
 LDCM0469  CL76 PaTu 8988t C139(0.74); C152(0.74)  LDD1348  [17]
 LDCM0470  CL77 PaTu 8988t C139(0.79); C152(0.79)  LDD1349  [17]
 LDCM0471  CL78 PaTu 8988t C139(0.57); C152(0.57)  LDD1350  [17]
 LDCM0472  CL79 PaTu 8988t C139(0.40); C152(0.40)  LDD1351  [17]
 LDCM0473  CL8 PaTu 8988t C139(1.37); C152(1.37)  LDD1352  [17]
 LDCM0474  CL80 PaTu 8988t C139(0.91); C152(0.91)  LDD1353  [17]
 LDCM0475  CL81 PaTu 8988t C139(1.17); C152(1.17)  LDD1354  [17]
 LDCM0476  CL82 PaTu 8988t C139(0.60); C152(0.60)  LDD1355  [17]
 LDCM0477  CL83 PaTu 8988t C139(0.86); C152(0.86)  LDD1356  [17]
 LDCM0478  CL84 PaTu 8988t C139(0.98); C152(0.98)  LDD1357  [17]
 LDCM0479  CL85 PaTu 8988t C139(0.87); C152(0.87)  LDD1358  [17]
 LDCM0480  CL86 PaTu 8988t C139(1.30); C152(1.30)  LDD1359  [17]
 LDCM0481  CL87 PaTu 8988t C139(1.79); C152(1.79)  LDD1360  [17]
 LDCM0482  CL88 PaTu 8988t C139(1.73); C152(1.73)  LDD1361  [17]
 LDCM0483  CL89 PaTu 8988t C139(1.56); C152(1.56)  LDD1362  [17]
 LDCM0484  CL9 PaTu 8988t C139(3.09); C152(3.09)  LDD1363  [17]
 LDCM0485  CL90 PaTu 8988t C139(1.76); C152(1.76)  LDD1364  [17]
 LDCM0486  CL91 HEK-293T C93(1.01)  LDD1689  [16]
 LDCM0490  CL95 HEK-293T C93(0.98)  LDD1693  [16]
 LDCM0492  CL97 HEK-293T C93(1.26)  LDD1695  [16]
 LDCM0493  CL98 HEK-293T C93(0.85)  LDD1696  [16]
 LDCM0625  F8 Ramos C73(1.32)  LDD2187  [18]
 LDCM0572  Fragment10 Ramos C73(0.42)  LDD2189  [18]
 LDCM0573  Fragment11 Ramos C73(0.01)  LDD2190  [18]
 LDCM0574  Fragment12 Ramos C73(0.50)  LDD2191  [18]
 LDCM0575  Fragment13 Ramos C73(1.18)  LDD2192  [18]
 LDCM0576  Fragment14 Ramos C73(0.57)  LDD2193  [18]
 LDCM0579  Fragment20 Ramos C73(0.38)  LDD2194  [18]
 LDCM0580  Fragment21 Ramos C73(0.91)  LDD2195  [18]
 LDCM0582  Fragment23 Ramos C73(0.37)  LDD2196  [18]
 LDCM0578  Fragment27 Ramos C73(1.40)  LDD2197  [18]
 LDCM0586  Fragment28 Ramos C73(0.68)  LDD2198  [18]
 LDCM0588  Fragment30 Ramos C73(0.99)  LDD2199  [18]
 LDCM0589  Fragment31 Ramos C73(1.02)  LDD2200  [18]
 LDCM0590  Fragment32 Ramos C73(0.42)  LDD2201  [18]
 LDCM0468  Fragment33 Ramos C73(0.95)  LDD2202  [18]
 LDCM0596  Fragment38 Ramos C73(1.49)  LDD2203  [18]
 LDCM0566  Fragment4 Ramos C73(0.50)  LDD2184  [18]
 LDCM0427  Fragment51 HEK-293T C93(2.20)  LDD1631  [16]
 LDCM0610  Fragment52 Ramos C73(1.12)  LDD2204  [18]
 LDCM0614  Fragment56 Ramos C73(1.03)  LDD2205  [18]
 LDCM0569  Fragment7 Ramos C73(0.45)  LDD2186  [18]
 LDCM0571  Fragment9 Ramos C73(0.26)  LDD2188  [18]
 LDCM0116  HHS-0101 DM93 Y69(0.89)  LDD0264  [5]
 LDCM0117  HHS-0201 DM93 Y69(0.71)  LDD0265  [5]
 LDCM0118  HHS-0301 DM93 Y69(0.68)  LDD0266  [5]
 LDCM0119  HHS-0401 DM93 Y69(0.77)  LDD0267  [5]
 LDCM0120  HHS-0701 DM93 Y69(0.97)  LDD0268  [5]
 LDCM0022  KB02 HEK-293T C93(0.87)  LDD1492  [16]
 LDCM0023  KB03 HEK-293T C93(1.12)  LDD1497  [16]
 LDCM0024  KB05 MKN-1 C73(0.83); C93(1.40)  LDD3331  [3]
 LDCM0110  W12 Hep-G2 C73(0.87)  LDD0237  [7]
 LDCM0111  W14 Hep-G2 C73(0.65)  LDD0238  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cell division cycle protein 23 homolog (CDC23) APC8/CDC23 family Q9UJX2
Deoxyhypusine synthase (DHPS) Deoxyhypusine synthase family P49366
NIF3-like protein 1 (NIF3L1) GTP cyclohydrolase I type 2/NIF3 family Q9GZT8
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Syntenin-1 (SDCBP) . O00560
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proto-oncogene c-Rel (REL) . Q04864

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
6 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
7 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
8 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
13 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
14 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
15 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
16 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
17 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
18 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578