Details of the Target

General Information of Target

Target ID LDTP05074
Target Name Tubulin alpha-1B chain (TUBA1B)
Gene Name TUBA1B
Gene ID 10376
Synonyms
Tubulin alpha-1B chain; EC 3.6.5.-; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain) [Cleaved into: Detyrosinated tubulin alpha-1B chain]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEGEEY
Target Bioclass
Enzyme
Family
Tubulin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Uniprot ID
P68363
Ensemble ID
ENST00000336023.9
HGNC ID
HGNC:18809
ChEMBL ID
CHEMBL3797010

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
IGROV1 SNV: p.T73I DBIA    Probe Info 
L428 SNV: p.L428F DBIA    Probe Info 
MOLT4 SNV: p.Q372K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 50 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P8
 Probe Info  		4.10  LDD0455  [1]
8RK64
 Probe Info  		N.A.  LDD0039  [2]
CHEMBL5175495
 Probe Info  		7.28  LDD0196  [3]
CY4
 Probe Info  		2.41  LDD0244  [4]
TH211
 Probe Info  		Y108(20.00); Y161(20.00); Y312(18.35); Y282(17.37)  LDD0257  [5]
TH214
 Probe Info  		Y108(20.00); Y262(20.00); Y272(20.00); Y312(20.00)  LDD0258  [5]
TH216
 Probe Info  		Y262(20.00); Y272(20.00); Y108(19.12); Y312(17.94)  LDD0259  [5]
YN-4
 Probe Info  		100.00  LDD0445  [6]
AZ-9
 Probe Info  		D46(1.01); D47(1.01); D345(1.15); E386(1.02)  LDD2208  [7]
Probe 1
 Probe Info  		Y108(82.90); Y161(115.83); Y224(41.31); Y262(11.16)  LDD3495  [8]
JZ128-DTB
 Probe Info  		N.A.  LDD0462  [9]
THZ1-DTB
 Probe Info  		C315(1.09); C347(1.08); C213(1.10); C200(1.03)  LDD0460  [9]
DA-P3
 Probe Info  		4.59  LDD0183  [10]
AHL-Pu-1
 Probe Info  		C295(7.28)  LDD0170  [11]
HHS-482
 Probe Info  		Y103(0.88); Y108(0.87); Y161(0.88); Y224(0.87)  LDD0285  [12]
HHS-475
 Probe Info  		Y408(0.57); Y103(0.64); Y399(0.65); Y262(0.67)  LDD0264  [13]
HHS-465
 Probe Info  		Y103(10.00); Y108(10.00); Y161(7.71); Y224(10.00)  LDD2237  [14]
DBIA
 Probe Info  		C315(3.01); C316(3.01); C376(2.26); C347(1.58)  LDD0080  [15]
AMP probe
 Probe Info  		K60(0.00); K370(0.00); K336(0.00); K338(0.00)  LDD0200  [16]
ATP probe
 Probe Info  		K60(0.00); K370(0.00); K336(0.00); K338(0.00)  LDD0199  [16]
N1
 Probe Info  		C347(0.00); C315(0.00); D345(0.00); K352(0.00)  LDD0245  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C376(0.00); C295(0.00); C4(0.00); C129(0.00)  LDD0038  [17]
IA-alkyne
 Probe Info  		C295(0.00); C347(0.00); C376(0.00); C129(0.00)  LDD0032  [18]
IPIAA_H
 Probe Info  		C129(0.00); C376(0.00); C295(0.00); C347(0.00)  LDD0030  [19]
Lodoacetamide azide
 Probe Info  		C376(0.00); C295(0.00); C315(0.00); C316(0.00)  LDD0037  [17]
2PCA
 Probe Info  		K352(0.00); R64(0.00); R264(0.00)  LDD0034  [20]
NAIA_4
 Probe Info  		C20(0.00); C129(0.00); C213(0.00); C347(0.00)  LDD2226  [21]
WYneO
 Probe Info  		N.A.  LDD0022  [22]
aHNE
 Probe Info  		C347(0.00); C295(0.00)  LDD0001  [22]
1d-yne
 Probe Info  		K352(0.00); K60(0.00); K370(0.00); K96(0.00)  LDD0356  [23]
Compound 10
 Probe Info  		C295(0.00); C315(0.00); C316(0.00); C347(0.00)  LDD2216  [24]
Compound 11
 Probe Info  		C295(0.00); C315(0.00); C316(0.00); C347(0.00)  LDD2213  [24]
IPM
 Probe Info  		C316(0.00); C295(0.00); C315(0.00); C376(0.00)  LDD0005  [22]
NHS
 Probe Info  		K60(0.00); K304(0.00); K394(0.00); K370(0.00)  LDD0010  [22]
OSF
 Probe Info  		Y272(0.00); H107(0.00); Y161(0.00); H266(0.00)  LDD0029  [25]
PF-06672131
 Probe Info  		C295(0.00); C347(0.00); C376(0.00)  LDD0017  [26]
SF
 Probe Info  		Y399(0.00); Y272(0.00); K336(0.00); K401(0.00)  LDD0028  [25]
VSF
 Probe Info  		C295(0.00); C376(0.00)  LDD0007  [22]
YN-1
 Probe Info  		N.A.  LDD0447  [6]
Phosphinate-6
 Probe Info  		C315(0.00); C25(0.00); C347(0.00); C305(0.00)  LDD0018  [27]
Ox-W18
 Probe Info  		N.A.  LDD2175  [28]
1c-yne
 Probe Info  		K352(0.00); K394(0.00); K326(0.00); K112(0.00)  LDD0228  [23]
Acrolein
 Probe Info  		N.A.  LDD0217  [29]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [29]
Methacrolein
 Probe Info  		N.A.  LDD0218  [29]
W1
 Probe Info  		C347(0.00); C316(0.00); C295(0.00); C315(0.00)  LDD0236  [30]
MPP-AC
 Probe Info  		N.A.  LDD0428  [31]
NAIA_5
 Probe Info  		C316(0.00); C315(0.00); C129(0.00); C347(0.00)  LDD2223  [21]
TER-AC
 Probe Info  		N.A.  LDD0426  [31]
TPP-AC
 Probe Info  		N.A.  LDD0427  [31]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		20.00  LDD0472  [32]
FFF probe13
 Probe Info  		16.44  LDD0476  [32]
FFF probe3
 Probe Info  		20.00  LDD0465  [32]
Diazir
 Probe Info  		E55(0.00); E77(0.00); Y357(0.00); E71(0.00)  LDD0011  [22]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C295(7.28)  LDD0170  [11]
 LDCM0630  CCW28-3 231MFP C376(1.56); C295(1.50); C347(1.38)  LDD2214  [33]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [29]
 LDCM0632  CL-Sc Hep-G2 C347(1.31); C315(1.00); C315(0.95); C347(0.88)  LDD2227  [21]
 LDCM0634  CY-0357 Hep-G2 C129(1.18); C376(1.16); C347(0.86); C347(0.80)  LDD2228  [21]
 LDCM0031  Epigallocatechin gallate HEK-293T 4.59  LDD0183  [10]
 LDCM0116  HHS-0101 DM93 Y408(0.57); Y103(0.64); Y399(0.65); Y262(0.67)  LDD0264  [13]
 LDCM0117  HHS-0201 DM93 Y399(0.53); Y161(0.53); Y103(0.60); Y312(0.60)  LDD0265  [13]
 LDCM0118  HHS-0301 DM93 Y312(0.52); Y262(0.52); Y399(0.61); Y272(0.64)  LDD0266  [13]
 LDCM0119  HHS-0401 DM93 Y272(0.39); Y312(0.42); Y262(0.44); Y399(0.49)  LDD0267  [13]
 LDCM0120  HHS-0701 DM93 Y103(0.40); Y272(0.47); Y262(0.52); Y312(0.53)  LDD0268  [13]
 LDCM0107  IAA HeLa N.A.  LDD0221  [29]
 LDCM0123  JWB131 DM93 Y103(0.88); Y108(0.87); Y161(0.88); Y224(0.87)  LDD0285  [12]
 LDCM0124  JWB142 DM93 Y103(0.60); Y108(0.59); Y161(0.60); Y224(0.61)  LDD0286  [12]
 LDCM0125  JWB146 DM93 Y103(1.12); Y108(1.13); Y161(1.08); Y224(1.11)  LDD0287  [12]
 LDCM0126  JWB150 DM93 Y103(2.30); Y108(2.21); Y161(2.31); Y224(2.46)  LDD0288  [12]
 LDCM0127  JWB152 DM93 Y103(1.74); Y108(1.71); Y161(1.62); Y224(1.83)  LDD0289  [12]
 LDCM0128  JWB198 DM93 Y103(1.06); Y108(1.06); Y161(1.04); Y224(1.02)  LDD0290  [12]
 LDCM0129  JWB202 DM93 Y103(0.39); Y108(0.39); Y161(0.37); Y224(0.43)  LDD0291  [12]
 LDCM0130  JWB211 DM93 Y103(0.91); Y108(0.89); Y161(0.90); Y224(0.89)  LDD0292  [12]
 LDCM0179  JZ128 PC-3 N.A.  LDD0462  [9]
 LDCM0022  KB02 HCT 116 C315(3.01); C316(3.01); C376(2.26); C347(1.58)  LDD0080  [15]
 LDCM0023  KB03 HCT 116 C315(8.72); C316(8.72); C376(4.03); C347(3.83)  LDD0081  [15]
 LDCM0024  KB05 HCT 116 C315(7.25); C316(7.25); C376(2.51); C347(1.58)  LDD0082  [15]
 LDCM0109  NEM HeLa N.A.  LDD0223  [29]
 LDCM0627  NUDT7-COV-1 HEK-293T C347(1.59)  LDD2206  [34]
 LDCM0628  OTUB2-COV-1 HEK-293T C347(0.96); C347(0.89); C347(0.84); C347(0.23)  LDD2207  [34]
 LDCM0021  THZ1 HeLa S3 C315(1.09); C347(1.08); C213(1.10); C200(1.03)  LDD0460  [9]
 LDCM0110  W12 Hep-G2 H107(2.12); Y108(2.23); C347(2.49)  LDD0237  [30]
 LDCM0111  W14 Hep-G2 C347(1.30); H107(1.70)  LDD0238  [30]
 LDCM0112  W16 Hep-G2 K338(0.63); K336(0.66); K370(0.71); Q372(0.71)  LDD0239  [30]
 LDCM0113  W17 Hep-G2 C347(0.67); Y108(1.07)  LDD0240  [30]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
Alpha-synuclein (SNCA) Synuclein family P37840
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein BRICK1 (BRK1) BRK1 family Q8WUW1
T-complex protein 1 subunit theta (CCT8) TCP-1 chaperonin family P50990
Tubulin beta-3 chain (TUBB3) Tubulin family Q13509
Type-1 angiotensin II receptor-associated protein (AGTRAP) . Q6RW13

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 4 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Epothilone D Small molecular drug DB01873
Patupilone Small molecular drug DB03010
2-mercapto-n-[12310-tetramethoxy-9-oxo-5679-tetrahydro-benzo[A]Heptalen-7-yl]Acetamide . DB07574
Cyt997 . DB05147

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-Terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos. J Am Chem Soc. 2020 Sep 30;142(39):16825-16841. doi: 10.1021/jacs.0c07726. Epub 2020 Sep 18.
Mass spectrometry data entry: PXD021557 , PXD015828
3 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
4 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
7 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
8 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
9 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
10 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
11 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
12 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
13 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
14 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
15 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
16 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
17 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
18 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
19 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
20 Unveiling the Multifaceted Capabilities of Endophytic Aspergillus flavus Isolated from Annona squamosa Fruit Peels against Staphylococcus Isolates and HCoV 229E-In Vitro and In Silico Investigations. Pharmaceuticals (Basel). 2024 May 19;17(5):656. doi: 10.3390/ph17050656.
Mass spectrometry data entry: PXD013019
21 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
22 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
23 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
24 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
25 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
26 Site-Specific Activity-Based Protein Profiling Using Phosphonate Handles. Mol Cell Proteomics. 2023 Jan;22(1):100455. doi: 10.1016/j.mcpro.2022.100455. Epub 2022 Nov 24.
Mass spectrometry data entry: PXD036569
27 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
28 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
29 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
30 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
31 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
32 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
33 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
34 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.