Details of the Target

General Information of Target

Target ID LDTP11906
Target Name Phosphatidylinositol glycan anchor biosynthesis class U protein (PIGU)
Gene Name PIGU
Gene ID 128869
Synonyms
CDC91L1; Phosphatidylinositol glycan anchor biosynthesis class U protein; Cell division cycle protein 91-like 1; Protein CDC91-like 1; GPI transamidase component PIG-U
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKL
LNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPE
KRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYREQWSQ
RFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLR
IGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTAAPLTMTMCLPDLKEIQRAVKLWVRS
LDAQSVYVATDSESYVPELQQLFKGKVKVVSLKPEVAQVDLYILGQADHFIGNCVSSFTA
FVKRERDLQGRPSSFFGMDRPPKLRDEF
Target Type
Literature-reported
Target Bioclass
Other
Family
PIGU family
Subcellular location
Endoplasmic reticulum membrane
Function Component of the GPI transamidase complex, necessary for transfer of GPI to proteins. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI.
TTD ID
T00088
Uniprot ID
Q9H490
DrugMap ID
TT2LHI6
Ensemble ID
ENST00000217446.8
HGNC ID
HGNC:15791

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.S39R .
HT SNV: p.D252G .
HT115 SNV: p.Y71H .
RL952 SNV: p.V201A .
TOV21G SNV: p.I399T .
WM1552C SNV: p.G60V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HDSF-alk
 Probe Info  		2.90  LDD0197  [1]
Jackson_1
 Probe Info  		20.00  LDD0122  [2]
TH211
 Probe Info  		Y119(20.00)  LDD0260  [3]
m-APA
 Probe Info  		12.15  LDD0403  [4]
DBIA
 Probe Info  		C154(1.48)  LDD3365  [5]
PAL-AfBPP Probe
Click To Hide/Show 32 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C040
 Probe Info  		10.63  LDD1740  [6]
C091
 Probe Info  		17.15  LDD1782  [6]
C092
 Probe Info  		26.17  LDD1783  [6]
C094
 Probe Info  		42.22  LDD1785  [6]
C153
 Probe Info  		15.67  LDD1834  [6]
C161
 Probe Info  		10.78  LDD1841  [6]
C187
 Probe Info  		20.11  LDD1865  [6]
C218
 Probe Info  		18.64  LDD1892  [6]
C219
 Probe Info  		6.92  LDD1893  [6]
C220
 Probe Info  		22.94  LDD1894  [6]
C228
 Probe Info  		16.22  LDD1901  [6]
C231
 Probe Info  		24.59  LDD1904  [6]
C233
 Probe Info  		10.27  LDD1906  [6]
C235
 Probe Info  		25.28  LDD1908  [6]
C246
 Probe Info  		16.22  LDD1919  [6]
C278
 Probe Info  		54.95  LDD1948  [6]
C282
 Probe Info  		16.45  LDD1952  [6]
C285
 Probe Info  		19.16  LDD1955  [6]
C287
 Probe Info  		9.38  LDD1957  [6]
C289
 Probe Info  		33.59  LDD1959  [6]
C307
 Probe Info  		5.54  LDD1975  [6]
C310
 Probe Info  		13.83  LDD1977  [6]
C348
 Probe Info  		10.27  LDD2009  [6]
C349
 Probe Info  		9.25  LDD2010  [6]
C350
 Probe Info  		41.07  LDD2011  [6]
C388
 Probe Info  		37.01  LDD2047  [6]
FFF probe11
 Probe Info  		20.00  LDD0471  [7]
FFF probe13
 Probe Info  		20.00  LDD0475  [7]
FFF probe3
 Probe Info  		19.99  LDD0464  [7]
STS-2
 Probe Info  		N.A.  LDD0138  [8]
A-DA
 Probe Info  		2.19  LDD0145  [9]
OEA-DA
 Probe Info  		9.85  LDD0046  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C154(0.97)  LDD1507  [11]
 LDCM0237  AC12 HEK-293T C154(1.22)  LDD1510  [11]
 LDCM0259  AC14 HEK-293T C154(0.97)  LDD1512  [11]
 LDCM0276  AC17 HEK-293T C154(0.94)  LDD1515  [11]
 LDCM0280  AC20 HEK-293T C154(0.97)  LDD1519  [11]
 LDCM0281  AC21 HEK-293T C154(0.89)  LDD1520  [11]
 LDCM0282  AC22 HEK-293T C154(0.99)  LDD1521  [11]
 LDCM0285  AC25 HEK-293T C154(1.12)  LDD1524  [11]
 LDCM0288  AC28 HEK-293T C154(0.98)  LDD1527  [11]
 LDCM0289  AC29 HEK-293T C154(0.91)  LDD1528  [11]
 LDCM0291  AC30 HEK-293T C154(1.03)  LDD1530  [11]
 LDCM0294  AC33 HEK-293T C154(0.97)  LDD1533  [11]
 LDCM0297  AC36 HEK-293T C154(0.94)  LDD1536  [11]
 LDCM0298  AC37 HEK-293T C154(0.80)  LDD1537  [11]
 LDCM0299  AC38 HEK-293T C154(1.02)  LDD1538  [11]
 LDCM0301  AC4 HEK-293T C154(1.16)  LDD1540  [11]
 LDCM0303  AC41 HEK-293T C154(1.08)  LDD1542  [11]
 LDCM0306  AC44 HEK-293T C154(1.16)  LDD1545  [11]
 LDCM0307  AC45 HEK-293T C154(0.91)  LDD1546  [11]
 LDCM0308  AC46 HEK-293T C154(0.97)  LDD1547  [11]
 LDCM0311  AC49 HEK-293T C154(0.99)  LDD1550  [11]
 LDCM0312  AC5 HEK-293T C154(0.85)  LDD1551  [11]
 LDCM0315  AC52 HEK-293T C154(1.10)  LDD1554  [11]
 LDCM0316  AC53 HEK-293T C154(0.87)  LDD1555  [11]
 LDCM0317  AC54 HEK-293T C154(0.99)  LDD1556  [11]
 LDCM0320  AC57 HEK-293T C154(0.98)  LDD1559  [11]
 LDCM0323  AC6 HEK-293T C154(1.02)  LDD1562  [11]
 LDCM0324  AC60 HEK-293T C154(1.18)  LDD1563  [11]
 LDCM0325  AC61 HEK-293T C154(0.99)  LDD1564  [11]
 LDCM0326  AC62 HEK-293T C154(1.24)  LDD1565  [11]
 LDCM0248  AKOS034007472 HEK-293T C154(0.86)  LDD1511  [11]
 LDCM0356  AKOS034007680 HEK-293T C154(1.00)  LDD1570  [11]
 LDCM0156  Aniline NCI-H1299 12.15  LDD0403  [4]
 LDCM0367  CL1 HEK-293T C154(1.24)  LDD1571  [11]
 LDCM0368  CL10 HEK-293T C154(2.15)  LDD1572  [11]
 LDCM0370  CL101 HEK-293T C154(0.99)  LDD1574  [11]
 LDCM0372  CL103 HEK-293T C154(1.12)  LDD1576  [11]
 LDCM0374  CL105 HEK-293T C154(1.40)  LDD1578  [11]
 LDCM0376  CL107 HEK-293T C154(1.07)  LDD1580  [11]
 LDCM0378  CL109 HEK-293T C154(1.16)  LDD1582  [11]
 LDCM0381  CL111 HEK-293T C154(1.35)  LDD1585  [11]
 LDCM0383  CL113 HEK-293T C154(1.18)  LDD1587  [11]
 LDCM0385  CL115 HEK-293T C154(1.02)  LDD1589  [11]
 LDCM0387  CL117 HEK-293T C154(0.87)  LDD1591  [11]
 LDCM0389  CL119 HEK-293T C154(1.00)  LDD1593  [11]
 LDCM0392  CL121 HEK-293T C154(0.94)  LDD1596  [11]
 LDCM0394  CL123 HEK-293T C154(1.62)  LDD1598  [11]
 LDCM0396  CL125 HEK-293T C154(1.21)  LDD1600  [11]
 LDCM0398  CL127 HEK-293T C154(1.25)  LDD1602  [11]
 LDCM0400  CL13 HEK-293T C154(1.04)  LDD1604  [11]
 LDCM0402  CL15 HEK-293T C154(2.16)  LDD1606  [11]
 LDCM0404  CL17 HEK-293T C154(1.93)  LDD1608  [11]
 LDCM0408  CL20 HEK-293T C154(1.07)  LDD1612  [11]
 LDCM0409  CL21 HEK-293T C154(1.12)  LDD1613  [11]
 LDCM0410  CL22 HEK-293T C154(1.11)  LDD1614  [11]
 LDCM0413  CL25 HEK-293T C154(1.36)  LDD1617  [11]
 LDCM0415  CL27 HEK-293T C154(1.09)  LDD1619  [11]
 LDCM0417  CL29 HEK-293T C154(1.00)  LDD1621  [11]
 LDCM0418  CL3 HEK-293T C154(1.59)  LDD1622  [11]
 LDCM0421  CL32 HEK-293T C154(1.15)  LDD1625  [11]
 LDCM0422  CL33 HEK-293T C154(1.59)  LDD1626  [11]
 LDCM0423  CL34 HEK-293T C154(1.21)  LDD1627  [11]
 LDCM0426  CL37 HEK-293T C154(1.24)  LDD1630  [11]
 LDCM0428  CL39 HEK-293T C154(1.14)  LDD1632  [11]
 LDCM0431  CL41 HEK-293T C154(1.27)  LDD1635  [11]
 LDCM0434  CL44 HEK-293T C154(0.99)  LDD1638  [11]
 LDCM0435  CL45 HEK-293T C154(0.90)  LDD1639  [11]
 LDCM0436  CL46 HEK-293T C154(0.95)  LDD1640  [11]
 LDCM0439  CL49 HEK-293T C154(1.46)  LDD1643  [11]
 LDCM0440  CL5 HEK-293T C154(1.19)  LDD1644  [11]
 LDCM0444  CL53 HEK-293T C154(1.39)  LDD1647  [11]
 LDCM0447  CL56 HEK-293T C154(1.14)  LDD1650  [11]
 LDCM0448  CL57 HEK-293T C154(1.12)  LDD1651  [11]
 LDCM0449  CL58 HEK-293T C154(1.17)  LDD1652  [11]
 LDCM0453  CL61 HEK-293T C154(1.52)  LDD1656  [11]
 LDCM0455  CL63 HEK-293T C154(0.96)  LDD1658  [11]
 LDCM0457  CL65 HEK-293T C154(1.13)  LDD1660  [11]
 LDCM0460  CL68 HEK-293T C154(1.17)  LDD1663  [11]
 LDCM0461  CL69 HEK-293T C154(1.07)  LDD1664  [11]
 LDCM0463  CL70 HEK-293T C154(1.09)  LDD1666  [11]
 LDCM0466  CL73 HEK-293T C154(1.42)  LDD1669  [11]
 LDCM0470  CL77 HEK-293T C154(1.27)  LDD1673  [11]
 LDCM0473  CL8 HEK-293T C154(3.30)  LDD1676  [11]
 LDCM0474  CL80 HEK-293T C154(1.04)  LDD1677  [11]
 LDCM0475  CL81 HEK-293T C154(0.92)  LDD1678  [11]
 LDCM0476  CL82 HEK-293T C154(0.98)  LDD1679  [11]
 LDCM0479  CL85 HEK-293T C154(1.43)  LDD1682  [11]
 LDCM0481  CL87 HEK-293T C154(1.29)  LDD1684  [11]
 LDCM0483  CL89 HEK-293T C154(1.18)  LDD1686  [11]
 LDCM0484  CL9 HEK-293T C154(1.07)  LDD1687  [11]
 LDCM0487  CL92 HEK-293T C154(1.46)  LDD1690  [11]
 LDCM0488  CL93 HEK-293T C154(1.24)  LDD1691  [11]
 LDCM0489  CL94 HEK-293T C154(1.17)  LDD1692  [11]
 LDCM0492  CL97 HEK-293T C154(1.89)  LDD1695  [11]
 LDCM0494  CL99 HEK-293T C154(1.01)  LDD1697  [11]
 LDCM0495  E2913 HEK-293T C154(1.01)  LDD1698  [11]
 LDCM0468  Fragment33 HEK-293T C154(0.96)  LDD1671  [11]
 LDCM0022  KB02 HEK-293T C154(0.94)  LDD1492  [11]
 LDCM0023  KB03 HEK-293T C154(0.95)  LDD1497  [11]
 LDCM0024  KB05 OVCAR-3 C154(1.48)  LDD3365  [5]
 LDCM0084  Ro 48-8071 A-549 2.19  LDD0145  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein KASH5 (KASH5) . Q8N6L0

References

1 Fatty Acyl Sulfonyl Fluoride as an Activity-Based Probe for Profiling Fatty Acid-Associated Proteins in Living Cells. Chembiochem. 2022 Feb 16;23(4):e202100628. doi: 10.1002/cbic.202100628. Epub 2021 Dec 30.
2 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
7 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
8 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
9 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
10 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402