Probe Name	IA-alkyne					Probe Info
Probe concentration	100 uM
Criteria	Quantification: Probe vs (Probe+Competitor)
Quantitative Method	Isotope tag
Competitor Name	Fragment31					Competitor Info
Competitor Concentration	200 uM
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Breast
	Disease Model		Breast adenocarcinoma [ICD-11:2C60]
	Model Name		Human breast adenocarcinoma cells (MDA-MB-231)

Target ID	Target Name				Binding Sites	Binding Ratio
LDTP00701	D-3-phosphoglycerate dehydrogenase (PHGDH)				C369	20.0
LDTP17247	5'-nucleotidase domain-containing protein 1 (NT5DC1)				C119	3.1
LDTP10973	Thioredoxin, mitochondrial (TXN2)				C90	1.4
LDTP07407	Desumoylating isopeptidase 1 (DESI1)				C108	1.2
LDTP02502	Thioredoxin (TXN)				C32	1.2
LDTP00601	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (OGT)				C620	1.1
LDTP03496	Protein PML (PML)				C389	1.1
LDTP06314	Protein disulfide-isomerase A6 (PDIA6)				C190	1.0
LDTP03291	Acetyl-CoA acetyltransferase, mitochondrial (ACAT1)				C413	1.0
LDTP12405	Fructose-2,6-bisphosphatase TIGAR (TIGAR)				C161	1.0
LDTP02611	Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2)				C331	1.0
LDTP01220	Mitochondrial-processing peptidase subunit beta (PMPCB)				C265	1.0
LDTP09126	Probable aminopeptidase NPEPL1 (NPEPL1)				C81	1.0
LDTP12960	[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial (PDP1)				C149	1.0
LDTP03521	Protein disulfide-isomerase A3 (PDIA3)				C57	0.9
LDTP03998	3-ketoacyl-CoA thiolase, mitochondrial (ACAA2)				C92	0.8
LDTP10177	Medium-chain acyl-CoA ligase ACSF2, mitochondrial (ACSF2)				C64	0.8
LDTP02677	Protein disulfide-isomerase A4 (PDIA4)				C206	0.8
LDTP10878	Parkinson disease protein 7 (PARK7)				C106	0.8
LDTP02765	Aldo-keto reductase family 1 member B1 (AKR1B1)				C299	0.8
LDTP02038	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)				C152	0.8
LDTP03521	Protein disulfide-isomerase A3 (PDIA3)				C406	0.8
LDTP02677	Protein disulfide-isomerase A4 (PDIA4)				C555	0.8
LDTP05128	Glutathione S-transferase omega-1 (GSTO1)				C32	0.8
LDTP09067	Thioredoxin domain-containing protein 5 (TXNDC5)				C350	0.8
LDTP03291	Acetyl-CoA acetyltransferase, mitochondrial (ACAT1)				C126	0.7
LDTP10386	ERO1-like protein alpha (ERO1A)				C241	0.7
LDTP06314	Protein disulfide-isomerase A6 (PDIA6)				C55	0.7
LDTP18123	Isochorismatase domain-containing protein 2 (ISOC2)				C114	0.7
LDTP02022	Thymidine kinase, cytosolic (TK1)				C66	0.7
LDTP01141	E3 ubiquitin-protein ligase BRE1B (RNF40)				C890	0.7
LDTP09412	Serine/threonine-protein kinase Nek9 (NEK9)				C623	0.7
LDTP05289	AMP deaminase 2 (AMPD2)				C107	0.7
LDTP08436	Interferon regulatory factor 2-binding protein 1 (IRF2BP1)				C363	0.7
LDTP02329	3-ketoacyl-CoA thiolase, peroxisomal (ACAA1)				C381	0.7
LDTP12627	Peroxisomal 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing] (DECR2)				C22	0.7
LDTP03786	Glutaredoxin-1 (GLRX)				C23	0.6
LDTP03291	Acetyl-CoA acetyltransferase, mitochondrial (ACAT1)				C119	0.6
LDTP10386	ERO1-like protein alpha (ERO1A)				C37	0.6
LDTP02611	Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2)				C140	0.6
LDTP05859	Integrin-linked protein kinase (ILK)				C346	0.6
LDTP05480	Amidophosphoribosyltransferase (PPAT)				C100	0.6
LDTP12428	Omega-amidase NIT2 (NIT2)				C153	0.6
LDTP05912	Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1)				C113	0.6
LDTP06211	Caspase-8 (CASP8)				C360	0.5
LDTP02552	Glycogen phosphorylase, brain form (PYGB)				C326	0.5
LDTP03316	DNA replication licensing factor MCM3 (MCM3)				C119	0.5
LDTP11059	ADP-ribose glycohydrolase MACROD1 (MACROD1)				C186	0.5
LDTP15370	Pseudouridylate synthase RPUSD2 (RPUSD2)				C246	0.4

Target ID	Target Name				Binding Sites	Binding Ratio
LDTP03777	Nuclear pore complex protein Nup214 (NUP214)				C728	0.7
LDTP11103	Thioredoxin domain-containing protein 17 (TXNDC17)				C43	0.6
LDTP00244	Chloride intracellular channel protein 1 (CLIC1)				C24	0.6
LDTP14222	Chloride intracellular channel protein 4 (CLIC4)				C35	0.6
LDTP05591	Nuclear cap-binding protein subunit 1 (NCBP1)				C44	0.6
LDTP08315	THO complex subunit 6 homolog (THOC6)				C35	0.5
LDTP01689	Chloride intracellular channel protein 3 (CLIC3)				C22	0.5
LDTP10642	Chloride intracellular channel protein 6 (CLIC6)				C487	0.5

Target ID	Target Name				Binding Sites	Binding Ratio
LDTP03969	Signal transducer and activator of transcription 1-alpha/beta (STAT1)				C492	0.8
LDTP09311	SWI/SNF complex subunit SMARCC2 (SMARCC2)				C145	0.8
LDTP06341	Non-POU domain-containing octamer-binding protein (NONO)				C145	0.7

Target ID	Target Name				Binding Sites	Binding Ratio
LDTP06281	Condensin complex subunit 1 (NCAPD2)				C439	20.0
LDTP10843	MMS19 nucleotide excision repair protein homolog (MMS19)				C848	20.0
LDTP10843	MMS19 nucleotide excision repair protein homolog (MMS19)				C819	20.0
LDTP12412	Reticulon-4 (RTN4)				C1101	12.8
LDTP06684	Sister chromatid cohesion protein PDS5 homolog A (PDS5A)				C532	5.1
LDTP07755	Dynamin-binding protein (DNMBP)				C691	2.3
LDTP11197	Mini-chromosome maintenance complex-binding protein (MCMBP)				C325	1.9
LDTP15701	EF-hand domain-containing protein D2 (EFHD2)				C172	1.6
LDTP13622	NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (NFU1)				C213	1.5
LDTP03967	Lamina-associated polypeptide 2, isoform alpha (TMPO)				C561	1.3
LDTP04514	Heterogeneous nuclear ribonucleoprotein F (HNRNPF)				C267	1.2
LDTP10657	Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 (ARAP1)				C900	1.1
LDTP06244	Nuclear mitotic apparatus protein 1 (NUMA1)				C961	1.1
LDTP13964	MOB-like protein phocein (MOB4)				C134	1.0
LDTP00463	Interferon-induced protein with tetratricopeptide repeats 3 (IFIT3)				C343	0.9
LDTP06362	Leucine-rich repeat-containing protein 41 (LRRC41)				C297	0.9
LDTP05772	FAS-associated death domain protein (FADD)				C98	0.9
LDTP04952	Heterogeneous nuclear ribonucleoprotein K (HNRNPK)				C132	0.9
LDTP10771	Mediator of RNA polymerase II transcription subunit 15 (MED15)				C618	0.9
LDTP10613	Dedicator of cytokinesis protein 7 (DOCK7)				C2125	0.8
LDTP10781	NudC domain-containing protein 1 (NUDCD1)				C376	0.8
LDTP11235	Protein PAXX (PAXX)				C180	0.8
LDTP05770	Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 (AIMP2)				C291	0.8
LDTP12669	Mediator of RNA polymerase II transcription subunit 17 (MED17)				C649	0.8
LDTP13580	Coronin-1C (CORO1C)				C420	0.8
LDTP18494	Tropomodulin-3 (TMOD3)				C132	0.7
LDTP06366	Poly(rC)-binding protein 1 (PCBP1)				C194	0.7
LDTP04560	Cytosolic Fe-S cluster assembly factor NUBP1 (NUBP1)				C235	0.7
LDTP03615	Heterogeneous nuclear ribonucleoprotein H (HNRNPH1)				C267	0.7
LDTP10864	Calponin-2 (CNN2)				C164	0.7
LDTP12443	Anillin (ANLN)				C712	0.7
LDTP01011	Protein diaphanous homolog 1 (DIAPH1)				C1227	0.6
LDTP09821	Stalled ribosome sensor GCN1 (GCN1)				C1692	0.6
LDTP12651	TBC1 domain family member 23 (TBC1D23)				C283	0.6
LDTP12571	LanC-like protein 2 (LANCL2)				C187	0.5
LDTP04201	T-complex protein 1 subunit epsilon (CCT5)				C253	0.5
LDTP13088	Protein IMPACT (IMPACT)				C195	0.5

1	Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.