Details of the Target

General Information of Target

Target ID LDTP09126
Target Name Probable aminopeptidase NPEPL1 (NPEPL1)
Gene Name NPEPL1
Gene ID 79716
Synonyms
KIAA1974; Probable aminopeptidase NPEPL1; EC 3.4.11.-; Aminopeptidase-like 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MANVGLQFQASAGDSDPQSRPLLLLGQLHHLHRVPWSHVRGKLQPRVTEELWQAALSTLN
PNPTDSCPLYLNYATVAALPCRVSRHNSPSAAHFITRLVRTCLPPGAHRCIVMVCEQPEV
FASACALARAFPLFTHRSGASRRLEKKTVTVEFFLVGQDNGPVEVSTLQCLANATDGVRL
AARIVDTPCNEMNTDTFLEEINKVGKELGIIPTIIRDEELKTRGFGGIYGVGKAALHPPA
LAVLSHTPDGATQTIAWVGKGIVYDTGGLSIKGKTTMPGMKRDCGGAAAVLGAFRAAIKQ
GFKDNLHAVFCLAENSVGPNATRPDDIHLLYSGKTVEINNTDAEGRLVLADGVSYACKDL
GADIILDMATLTGAQGIATGKYHAAVLTNSAEWEAACVKAGRKCGDLVHPLVYCPELHFS
EFTSAVADMKNSVADRDNSPSSCAGLFIASHIGFDWPGVWVHLDIAAPVHAGERATGFGV
ALLLALFGRASEDPLLNLVSPLGCEVDVEEGDLGRDSKRRRLV
Target Bioclass
Enzyme
Family
Peptidase M17 family
Function Probably catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
Uniprot ID
Q8NDH3
Ensemble ID
ENST00000356091.11
HGNC ID
HGNC:16244
ChEMBL ID
CHEMBL3831223

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO800 SNV: p.V412G; p.E416G DBIA    Probe Info 
HCT116 SNV: p.A449T .
HEC1B SNV: p.A481T .
HUH7 SNV: p.P278Q DBIA    Probe Info 
KP4 SNV: p.R142Q DBIA    Probe Info 
NCIH1944 SNV: p.D359V DBIA    Probe Info 
NCIH2172 SNV: p.R282P DBIA    Probe Info 
SW403 Insertion: p.K399Ter DBIA    Probe Info 
TC71 SNV: p.G232S DBIA    Probe Info 
TOV21G SNV: p.P278Q DBIA    Probe Info 
UMUC3 SNV: p.D435N DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		12.26  LDD0402  [1]
STPyne
 Probe Info  		K274(6.48)  LDD0277  [2]
DBIA
 Probe Info  		C357(0.76)  LDD3310  [3]
THZ1-DTB
 Probe Info  		C67(1.03)  LDD0460  [4]
AHL-Pu-1
 Probe Info  		C81(2.13)  LDD0170  [5]
HHS-482
 Probe Info  		Y382(0.80)  LDD0285  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [7]
IPM
 Probe Info  		N.A.  LDD0025  [8]
TFBX
 Probe Info  		C102(0.00); C284(0.00)  LDD0148  [8]
Acrolein
 Probe Info  		N.A.  LDD0217  [9]
NAIA_5
 Probe Info  		C284(0.00); C189(0.00); C357(0.00); C102(0.00)  LDD2223  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C81(2.13)  LDD0170  [5]
 LDCM0156  Aniline NCI-H1299 11.58  LDD0403  [1]
 LDCM0632  CL-Sc Hep-G2 C189(1.51); C284(1.36); C357(0.89); C397(0.82)  LDD2227  [10]
 LDCM0634  CY-0357 Hep-G2 C357(0.99)  LDD2228  [10]
 LDCM0572  Fragment10 MDA-MB-231 C81(5.70)  LDD1465  [11]
 LDCM0574  Fragment12 MDA-MB-231 C81(20.00)  LDD1468  [11]
 LDCM0576  Fragment14 MDA-MB-231 C81(1.35)  LDD1471  [11]
 LDCM0580  Fragment21 MDA-MB-231 C81(1.55)  LDD1473  [11]
 LDCM0589  Fragment31 MDA-MB-231 C81(0.95)  LDD1477  [11]
 LDCM0596  Fragment38 MDA-MB-231 C81(2.01)  LDD1480  [11]
 LDCM0566  Fragment4 MDA-MB-231 C81(20.00)  LDD1461  [11]
 LDCM0614  Fragment56 MDA-MB-231 C81(0.99)  LDD1484  [11]
 LDCM0570  Fragment8 MDA-MB-231 C81(20.00)  LDD1462  [11]
 LDCM0123  JWB131 DM93 Y382(0.80)  LDD0285  [6]
 LDCM0124  JWB142 DM93 Y382(1.28)  LDD0286  [6]
 LDCM0125  JWB146 DM93 Y382(0.97)  LDD0287  [6]
 LDCM0126  JWB150 DM93 Y382(3.28)  LDD0288  [6]
 LDCM0127  JWB152 DM93 Y382(1.66)  LDD0289  [6]
 LDCM0128  JWB198 DM93 Y382(0.11)  LDD0290  [6]
 LDCM0129  JWB202 DM93 Y382(0.88)  LDD0291  [6]
 LDCM0130  JWB211 DM93 Y382(1.52)  LDD0292  [6]
 LDCM0022  KB02 MDA-MB-231 C81(20.00)  LDD1460  [11]
 LDCM0023  KB03 22RV1 C74(1.48)  LDD2660  [3]
 LDCM0024  KB05 COLO792 C357(0.76)  LDD3310  [3]
 LDCM0131  RA190 MM1.R C189(2.43)  LDD0304  [12]
 LDCM0021  THZ1 HeLa S3 C67(1.03)  LDD0460  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Caspase-6 (CASP6) Peptidase C14A family P55212
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
MyoD family inhibitor (MDFI) MDFI family Q99750
Huntingtin-interacting protein 1 (HIP1) SLA2 family O00291
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-globin transcription factor CP2 (TFCP2) Grh/CP2 family Q12800

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
5 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
6 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
12 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.