Details of the Target

General Information of Target

Target ID LDTP05070
Target Name Ubiquitin-conjugating enzyme E2 L3 (UBE2L3)
Gene Name UBE2L3
Gene ID 7332
Synonyms
UBCE7; UBCH7; Ubiquitin-conjugating enzyme E2 L3; EC 2.3.2.23; E2 ubiquitin-conjugating enzyme L3; L-UBC; UbcH7; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAE
YPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHP
LRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD
Target Bioclass
Enzyme
Family
Ubiquitin-conjugating enzyme family
Subcellular location
Nucleus
Function
Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
Uniprot ID
P68036
Ensemble ID
ENST00000342192.9
HGNC ID
HGNC:12488
ChEMBL ID
CHEMBL4105871

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.S4N .
DU145 SNV: p.F57S .
HCT15 SNV: p.A2T .
MCC13 SNV: p.R122W .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 29 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
A-EBA
 Probe Info  		5.74  LDD0215  [2]
ONAyne
 Probe Info  		K131(10.00)  LDD0274  [3]
STPyne
 Probe Info  		K138(10.00); K145(4.42); K150(1.25); K64(4.86)  LDD0277  [3]
BTD
 Probe Info  		C86(2.19)  LDD1699  [4]
IPM
 Probe Info  		N.A.  LDD0241  [5]
Probe 1
 Probe Info  		Y129(111.30)  LDD3495  [6]
EA-probe
 Probe Info  		N.A.  LDD0440  [7]
HHS-475
 Probe Info  		Y75(0.35)  LDD0264  [8]
TFBX
 Probe Info  		C86(3.29)  LDD0312  [9]
ATP probe
 Probe Info  		K9(0.00); K73(0.00); K145(0.00); K146(0.00)  LDD0199  [10]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [11]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [12]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [13]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [13]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [11]
ATP probe
 Probe Info  		N.A.  LDD0035  [14]
NAIA_4
 Probe Info  		C86(0.00); C137(0.00)  LDD2226  [15]
Compound 10
 Probe Info  		N.A.  LDD2216  [16]
Compound 11
 Probe Info  		N.A.  LDD2213  [16]
NHS
 Probe Info  		K131(0.00); K73(0.00); K64(0.00)  LDD0010  [17]
SF
 Probe Info  		K67(0.00); K73(0.00)  LDD0028  [18]
VSF
 Probe Info  		N.A.  LDD0007  [17]
Ox-W18
 Probe Info  		N.A.  LDD2175  [19]
1c-yne
 Probe Info  		N.A.  LDD0228  [20]
W1
 Probe Info  		N.A.  LDD0236  [5]
AOyne
 Probe Info  		15.00  LDD0443  [21]
NAIA_5
 Probe Info  		N.A.  LDD2223  [15]
HHS-465
 Probe Info  		K73(0.00); Y75(0.00); Y147(0.00); K96(0.00)  LDD2240  [22]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe13
 Probe Info  		12.06  LDD0475  [23]
FFF probe2
 Probe Info  		5.22  LDD0463  [23]
DA-2
 Probe Info  		N.A.  LDD0073  [24]
STS-1
 Probe Info  		N.A.  LDD0068  [25]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C86(0.87)  LDD2142  [4]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C86(0.95)  LDD2112  [4]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C86(0.89)  LDD2117  [4]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C86(1.16)  LDD2103  [4]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C86(0.58)  LDD2132  [4]
 LDCM0026  4SU-RNA+native RNA HEK-293T C86(2.10)  LDD0372  [26]
 LDCM0563  Abegg_cp(+)-11 HeLa C86(2.21)  LDD0314  [9]
 LDCM0561  Abegg_cp(-)-10 HeLa C86(3.29)  LDD0312  [9]
 LDCM0562  Abegg_cp(-)-11 HeLa C86(2.13)  LDD0313  [9]
 LDCM0156  Aniline NCI-H1299 13.49  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C86(0.40)  LDD2091  [4]
 LDCM0630  CCW28-3 231MFP C86(1.83)  LDD2214  [27]
 LDCM0632  CL-Sc Hep-G2 C86(1.02); C86(0.62)  LDD2227  [15]
 LDCM0634  CY-0357 Hep-G2 C86(0.59)  LDD2228  [15]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C86(1.01)  LDD1702  [4]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [7]
 LDCM0625  F8 Ramos C86(2.12)  LDD2187  [28]
 LDCM0572  Fragment10 MDA-MB-231 C86(1.10)  LDD1389  [29]
 LDCM0573  Fragment11 Ramos C86(0.95)  LDD1392  [29]
 LDCM0574  Fragment12 Ramos C86(0.34)  LDD2191  [28]
 LDCM0575  Fragment13 MDA-MB-231 C86(1.09)  LDD1395  [29]
 LDCM0576  Fragment14 MDA-MB-231 C86(0.99)  LDD1397  [29]
 LDCM0577  Fragment15 Ramos C86(1.29)  LDD1400  [29]
 LDCM0579  Fragment20 MDA-MB-231 C86(1.14)  LDD1402  [29]
 LDCM0580  Fragment21 MDA-MB-231 C86(1.18)  LDD1404  [29]
 LDCM0581  Fragment22 Ramos C86(0.84)  LDD1407  [29]
 LDCM0582  Fragment23 MDA-MB-231 C86(7.38)  LDD1408  [29]
 LDCM0584  Fragment25 MDA-MB-231 C86(1.54)  LDD1411  [29]
 LDCM0585  Fragment26 Ramos C86(0.70)  LDD1412  [29]
 LDCM0578  Fragment27 MDA-MB-231 C86(1.21)  LDD1401  [29]
 LDCM0586  Fragment28 MDA-MB-231 C86(1.38)  LDD1415  [29]
 LDCM0587  Fragment29 MDA-MB-231 C86(0.90)  LDD1417  [29]
 LDCM0588  Fragment30 Ramos C86(1.45)  LDD1420  [29]
 LDCM0589  Fragment31 MDA-MB-231 C86(1.71)  LDD1421  [29]
 LDCM0590  Fragment32 MDA-MB-231 C86(1.06)  LDD1423  [29]
 LDCM0468  Fragment33 Ramos C86(0.88)  LDD1426  [29]
 LDCM0596  Fragment38 Ramos C86(1.51)  LDD1434  [29]
 LDCM0597  Fragment39 MDA-MB-231 C86(1.89)  LDD1435  [29]
 LDCM0566  Fragment4 MDA-MB-231 C86(1.21)  LDD1378  [29]
 LDCM0598  Fragment40 MDA-MB-231 C86(0.76)  LDD1436  [29]
 LDCM0599  Fragment41 MDA-MB-231 C86(9.75)  LDD1438  [29]
 LDCM0600  Fragment42 Ramos C86(0.83)  LDD1440  [29]
 LDCM0601  Fragment43 MDA-MB-231 C86(1.07)  LDD1441  [29]
 LDCM0604  Fragment46 MDA-MB-231 C86(1.15)  LDD1445  [29]
 LDCM0605  Fragment47 MDA-MB-231 C86(2.04)  LDD1446  [29]
 LDCM0607  Fragment49 MDA-MB-231 C86(1.87)  LDD1448  [29]
 LDCM0427  Fragment51 Ramos C86(1.01)  LDD1451  [29]
 LDCM0610  Fragment52 MDA-MB-231 C86(0.80)  LDD1452  [29]
 LDCM0611  Fragment53 MDA-MB-231 C86(1.02)  LDD1454  [29]
 LDCM0613  Fragment55 MDA-MB-231 C86(1.03)  LDD1457  [29]
 LDCM0614  Fragment56 MDA-MB-231 C86(1.54)  LDD1458  [29]
 LDCM0568  Fragment6 MDA-MB-231 C86(1.78)  LDD1382  [29]
 LDCM0569  Fragment7 MDA-MB-231 C86(0.91)  LDD1383  [29]
 LDCM0570  Fragment8 MDA-MB-231 C86(1.38)  LDD1385  [29]
 LDCM0571  Fragment9 MDA-MB-231 C86(0.84)  LDD1387  [29]
 LDCM0116  HHS-0101 DM93 Y75(0.35)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y75(0.59)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y75(1.04)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y75(1.20)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y75(0.99)  LDD0268  [8]
 LDCM0015  HNE MDA-MB-231 C86(1.02)  LDD0346  [28]
 LDCM0022  KB02 MDA-MB-231 C86(2.75)  LDD1374  [29]
 LDCM0023  KB03 MDA-MB-231 C86(1.15)  LDD1376  [29]
 LDCM0024  KB05 Ramos C86(4.81)  LDD1381  [29]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C86(1.23)  LDD2102  [4]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C86(0.91)  LDD2092  [4]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C86(1.34)  LDD2094  [4]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C86(0.62)  LDD2096  [4]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C86(1.31)  LDD2098  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C86(0.96)  LDD2099  [4]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C86(1.12)  LDD2100  [4]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C86(0.83)  LDD2104  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C86(1.00)  LDD2107  [4]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C86(0.73)  LDD2108  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C86(1.11)  LDD2109  [4]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C86(2.42)  LDD2110  [4]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C86(0.70)  LDD2114  [4]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C86(0.78); C17(1.61)  LDD2116  [4]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C86(0.73)  LDD2118  [4]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C86(0.87)  LDD2120  [4]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C86(0.98)  LDD2122  [4]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C86(0.75)  LDD2123  [4]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C86(0.77)  LDD2124  [4]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C86(0.76)  LDD2125  [4]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C86(0.90)  LDD2126  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C86(1.11)  LDD2127  [4]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C86(0.58)  LDD2134  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C86(0.85)  LDD2135  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C86(1.18)  LDD2136  [4]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C86(0.93)  LDD2137  [4]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C86(1.04)  LDD2141  [4]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C86(0.74)  LDD2143  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C86(1.10)  LDD2146  [4]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C86(1.01)  LDD2149  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C86(0.47)  LDD2150  [4]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C86(1.54)  LDD2153  [4]
 LDCM0627  NUDT7-COV-1 HEK-293T C86(1.16)  LDD2206  [30]
 LDCM0628  OTUB2-COV-1 HEK-293T C86(2.01); C86(0.77)  LDD2207  [30]
 LDCM0131  RA190 MM1.R C86(0.93)  LDD0304  [31]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
RanBP-type and C3HC4-type zinc finger-containing protein 1 (RBCK1) RBR family Q9BYM8
E3 ubiquitin-protein ligase ARIH1 (ARIH1) RBR family Q9Y4X5
E3 ubiquitin-protein ligase ARIH2 (ARIH2) RBR family O95376
E3 ubiquitin-protein ligase RNF144A (RNF144A) RBR family P50876
E3 ubiquitin-protein ligase RNF19B (RNF19B) RBR family Q6ZMZ0
E3 ubiquitin-protein ligase Midline-1 (MID1) TRIM/RBCC family O15344
E3 ubiquitin-protein ligase CHIP (STUB1) . Q9UNE7
E3 ubiquitin-protein ligase RNF182 (RNF182) . Q8N6D2
E3 ubiquitin-protein ligase RNF216 (RNF216) . Q9NWF9

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
6 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
7 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
12 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
13 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
14 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
15 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
16 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
17 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
18 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
19 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
20 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
21 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
22 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
23 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
24 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
25 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.
26 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
27 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
28 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
29 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
30 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
31 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.