Details of the Target

General Information of Target

Target ID LDTP06657
Target Name 2-hydroxyacylsphingosine 1-beta-galactosyltransferase (UGT8)
Gene Name UGT8
Gene ID 7368
Synonyms
CGT; UGT4; 2-hydroxyacylsphingosine 1-beta-galactosyltransferase; EC 2.4.1.47; Ceramide UDP-galactosyltransferase; Cerebroside synthase; UDP-galactose-ceramide galactosyltransferase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKSYTPYFILLWSAVGIAKAAKIIIVPPIMFESHMYIFKTLASALHERGHHTVFLLSEGR
DIAPSNHYSLQRYPGIFNSTTSDAFLQSKMRNIFSGRLTAIELFDILDHYTKNCDLMVGN
HALIQGLKKEKFDLLLVDPNDMCGFVIAHLLGVKYAVFSTGLWYPAEVGAPAPLAYVPEF
NSLLTDRMNLLQRMKNTGVYLISRLGVSFLVLPKYERIMQKYNLLPEKSMYDLVHGSSLW
MLCTDVALEFPRPTLPNVVYVGGILTKPASPLPEDLQRWVNGANEHGFVLVSFGAGVKYL
SEDIANKLAGALGRLPQKVIWRFSGPKPKNLGNNTKLIEWLPQNDLLGHSKIKAFLSHGG
LNSIFETIYHGVPVVGIPLFGDHYDTMTRVQAKGMGILLEWKTVTEKELYEALVKVINNP
SYRQRAQKLSEIHKDQPGHPVNRTIYWIDYIIRHNGAHHLRAAVHQISFCQYFLLDIAFV
LLLGAALLYFLLSWVTKFIYRKIKSLWSRNKHSTVNGHYHNGILNGKYKRNGHIKHEKKV
K
Target Bioclass
Enzyme
Family
UDP-glycosyltransferase family
Subcellular location
Membrane
Function
Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. Galactosylates both hydroxy- and non-hydroxy fatty acid-containing ceramides and diglycerides.
Uniprot ID
Q16880
Ensemble ID
ENST00000310836.11
HGNC ID
HGNC:12555
ChEMBL ID
CHEMBL4739855

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
CHEMBL5175495
 Probe Info  		8.50  LDD0196  [1]
OPA-S-S-alkyne
 Probe Info  		K195(1.49)  LDD3494  [2]
Acrolein
 Probe Info  		N.A.  LDD0227  [3]
STPyne
 Probe Info  		N.A.  LDD0009  [4]
AOyne
 Probe Info  		10.70  LDD0443  [5]
PAL-AfBPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C022
 Probe Info  		13.09  LDD1728  [6]
C082
 Probe Info  		5.54  LDD1774  [6]
C091
 Probe Info  		10.85  LDD1782  [6]
C092
 Probe Info  		17.15  LDD1783  [6]
C094
 Probe Info  		37.01  LDD1785  [6]
C106
 Probe Info  		30.06  LDD1793  [6]
C107
 Probe Info  		7.31  LDD1794  [6]
C108
 Probe Info  		8.82  LDD1795  [6]
C201
 Probe Info  		34.54  LDD1877  [6]
C206
 Probe Info  		15.56  LDD1881  [6]
C232
 Probe Info  		61.82  LDD1905  [6]
C234
 Probe Info  		11.71  LDD1907  [6]
C235
 Probe Info  		20.97  LDD1908  [6]
C251
 Probe Info  		28.05  LDD1924  [6]
C362
 Probe Info  		49.18  LDD2023  [6]
C363
 Probe Info  		22.47  LDD2024  [6]
C364
 Probe Info  		27.67  LDD2025  [6]
C366
 Probe Info  		5.31  LDD2027  [6]
C431
 Probe Info  		13.93  LDD2086  [6]
OEA-DA
 Probe Info  		15.06  LDD0046  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0109  NEM HeLa N.A.  LDD0227  [3]

References

1 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
2 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
7 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570