Details of the Target

General Information of Target

Target ID LDTP02940
Target Name Large ribosomal subunit protein eL33 (RPL35A)
Gene Name RPL35A
Gene ID 6165
Synonyms
Large ribosomal subunit protein eL33; 60S ribosomal protein L35a; Cell growth-inhibiting gene 33 protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTP
GGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI
Target Bioclass
Other
Family
Eukaryotic ribosomal protein eL33 family
Subcellular location
Cytoplasm
Function
Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for the proliferation and viability of hematopoietic cells.
Uniprot ID
P18077
Ensemble ID
ENST00000448864.6
HGNC ID
HGNC:10345

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 34 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		7.58  LDD0402  [1]
A-EBA
 Probe Info  		3.40  LDD0215  [2]
CY4
 Probe Info  		3.40  LDD0244  [3]
TH211
 Probe Info  		Y14(13.51); Y34(12.41)  LDD0260  [4]
C-Sul
 Probe Info  		4.11  LDD0066  [5]
TH216
 Probe Info  		Y14(20.00); Y34(11.22)  LDD0259  [4]
YN-4
 Probe Info  		100.00  LDD0445  [6]
ONAyne
 Probe Info  		N.A.  LDD0273  [7]
OPA-S-S-alkyne
 Probe Info  		K45(1.38); K95(3.10); K73(3.82)  LDD3494  [8]
Probe 1
 Probe Info  		Y14(21.96)  LDD3495  [9]
DBIA
 Probe Info  		C47(2.70)  LDD2260  [10]
BTD
 Probe Info  		C47(1.04)  LDD2089  [11]
HHS-482
 Probe Info  		Y34(0.81)  LDD0285  [12]
IPM
 Probe Info  		C47(3.67)  LDD1701  [11]
ATP probe
 Probe Info  		K95(0.00); K45(0.00); K63(0.00); K66(0.00)  LDD0199  [13]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [14]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [14]
ATP probe
 Probe Info  		K95(0.00); K66(0.00)  LDD0035  [15]
NAIA_4
 Probe Info  		N.A.  LDD2226  [16]
TFBX
 Probe Info  		N.A.  LDD0027  [17]
WYneN
 Probe Info  		N.A.  LDD0021  [18]
1d-yne
 Probe Info  		N.A.  LDD0356  [19]
Compound 10
 Probe Info  		N.A.  LDD2216  [20]
NHS
 Probe Info  		N.A.  LDD0010  [18]
OSF
 Probe Info  		N.A.  LDD0029  [21]
SF
 Probe Info  		Y42(0.00); Y34(0.00); K63(0.00); K95(0.00)  LDD0028  [21]
STPyne
 Probe Info  		K29(0.00); K95(0.00)  LDD0009  [18]
Ox-W18
 Probe Info  		W6(0.00); W71(0.00)  LDD2175  [22]
Acrolein
 Probe Info  		N.A.  LDD0217  [23]
Crotonaldehyde
 Probe Info  		H78(0.00); H24(0.00); C47(0.00)  LDD0219  [23]
Methacrolein
 Probe Info  		H78(0.00); H24(0.00)  LDD0218  [23]
W1
 Probe Info  		K45(0.00); R46(0.00); W71(0.00)  LDD0236  [24]
AOyne
 Probe Info  		6.00  LDD0443  [25]
NAIA_5
 Probe Info  		N.A.  LDD2223  [16]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C187
 Probe Info  		31.78  LDD1865  [26]
C191
 Probe Info  		23.43  LDD1868  [26]
C193
 Probe Info  		12.21  LDD1869  [26]
C313
 Probe Info  		23.75  LDD1980  [26]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C47(0.94)  LDD2142  [11]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C47(0.97)  LDD2112  [11]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C47(0.65)  LDD2095  [11]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C47(1.11)  LDD2130  [11]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C47(1.04)  LDD2117  [11]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C47(1.54)  LDD2152  [11]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C47(0.84)  LDD2132  [11]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C47(0.76)  LDD2131  [11]
 LDCM0545  Acetamide MDA-MB-231 C47(0.65)  LDD2138  [11]
 LDCM0498  BS-3668 MDA-MB-231 C47(0.71)  LDD2091  [11]
 LDCM0108  Chloroacetamide HeLa H24(0.00); H78(0.00); C47(0.00)  LDD0222  [23]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C47(1.65)  LDD1702  [11]
 LDCM0107  IAA HeLa H24(0.00); H78(0.00)  LDD0221  [23]
 LDCM0123  JWB131 DM93 Y34(0.81)  LDD0285  [12]
 LDCM0124  JWB142 DM93 Y34(0.60)  LDD0286  [12]
 LDCM0125  JWB146 DM93 Y34(1.06)  LDD0287  [12]
 LDCM0126  JWB150 DM93 Y34(1.64)  LDD0288  [12]
 LDCM0127  JWB152 DM93 Y34(1.29)  LDD0289  [12]
 LDCM0128  JWB198 DM93 Y34(1.30)  LDD0290  [12]
 LDCM0129  JWB202 DM93 Y34(0.45)  LDD0291  [12]
 LDCM0130  JWB211 DM93 Y34(0.92)  LDD0292  [12]
 LDCM0022  KB02 A-549 C47(2.70)  LDD2260  [10]
 LDCM0023  KB03 MDA-MB-231 C47(3.67)  LDD1701  [11]
 LDCM0024  KB05 A-549 C47(4.65)  LDD3094  [10]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C47(1.07)  LDD2102  [11]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C47(0.99)  LDD2121  [11]
 LDCM0109  NEM HeLa N.A.  LDD0223  [23]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C47(1.04)  LDD2089  [11]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C47(0.82)  LDD2092  [11]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C47(1.44)  LDD2093  [11]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C47(1.08)  LDD2094  [11]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C47(0.96)  LDD2096  [11]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C47(0.95)  LDD2097  [11]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C47(1.04)  LDD2098  [11]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C47(0.94)  LDD2099  [11]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C47(0.85)  LDD2100  [11]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C47(1.10)  LDD2104  [11]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C47(1.19)  LDD2105  [11]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C47(0.54)  LDD2106  [11]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C47(1.18)  LDD2107  [11]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C47(0.83)  LDD2108  [11]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C47(0.86)  LDD2109  [11]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C47(0.77)  LDD2114  [11]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C47(1.31)  LDD2116  [11]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C47(1.45)  LDD2118  [11]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C47(1.50)  LDD2119  [11]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C47(1.82)  LDD2122  [11]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C47(1.01)  LDD2123  [11]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C47(1.23)  LDD2126  [11]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C47(1.21)  LDD2127  [11]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C47(0.70)  LDD2128  [11]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C47(1.08)  LDD2129  [11]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C47(0.91)  LDD2135  [11]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C47(1.43)  LDD2136  [11]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C47(0.94)  LDD2137  [11]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C47(1.04)  LDD2140  [11]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C47(0.97)  LDD2141  [11]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C47(1.70)  LDD2144  [11]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C47(1.10)  LDD2145  [11]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C47(0.96)  LDD2146  [11]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C47(0.67)  LDD2150  [11]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C47(1.04)  LDD2151  [11]
 LDCM0110  W12 Hep-G2 R16(1.20); K45(1.86); R46(1.86)  LDD0237  [24]
 LDCM0112  W16 Hep-G2 K45(1.05); R46(1.05); R16(1.14)  LDD0239  [24]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone acetyltransferase KAT5 (KAT5) MYST (SAS/MOZ) family Q92993
Protein kinase C alpha type (PRKCA) AGC Ser/Thr protein kinase family P17252
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein gamma (YWHAG) 14-3-3 family P61981

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
6 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
7 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
8 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
9 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
10 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
11 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
12 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
13 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
14 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
15 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
16 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
17 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
18 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
19 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
20 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
21 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
22 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
23 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
24 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
25 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
26 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587