Details of the Target

General Information of Target

Target ID LDTP01514
Target Name NADH dehydrogenase 1 beta subcomplex subunit 4 (NDUFB4)
Gene Name NDUFB4
Gene ID 4710
Synonyms
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; Complex I-B15; CI-B15; NADH-ubiquinone oxidoreductase B15 subunit
3D Structure
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2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQLKREYLLQYNDPNRRGLI
ENPALLRWAYARTINVYPNFRPTPKNSLMGALCGFGPLIFIYYIIKTERDRKEKLIQEGK
LDRTFHLSY
Target Bioclass
Enzyme
Family
Complex I NDUFB4 subunit family
Subcellular location
Mitochondrion inner membrane
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Uniprot ID
O95168
Ensemble ID
ENST00000184266.3
HGNC ID
HGNC:7699
ChEMBL ID
CHEMBL2363065

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		11.46  LDD0402  [1]
CHEMBL5175495
 Probe Info  		14.48  LDD0196  [2]
N1
 Probe Info  		100.00  LDD0242  [3]
SAA-alkyne
 Probe Info  		1.11  LDD0252  [4]
TH211
 Probe Info  		Y6(8.34); Y77(8.98)  LDD0260  [5]
YN-1
 Probe Info  		100.00  LDD0444  [6]
Probe 1
 Probe Info  		Y23(12.70)  LDD3495  [7]
Alkyne-RA190
 Probe Info  		2.64  LDD0299  [8]
DBIA
 Probe Info  		C93(0.94)  LDD1507  [9]
SF
 Probe Info  		N.A.  LDD0028  [10]
AOyne
 Probe Info  		15.00  LDD0443  [11]
NAIA_5
 Probe Info  		N.A.  LDD2223  [12]
PAL-AfBPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C112
 Probe Info  		18.00  LDD1799  [13]
C134
 Probe Info  		20.11  LDD1816  [13]
C191
 Probe Info  		11.71  LDD1868  [13]
C201
 Probe Info  		26.91  LDD1877  [13]
C206
 Probe Info  		15.67  LDD1881  [13]
C211
 Probe Info  		8.06  LDD1885  [13]
C220
 Probe Info  		13.27  LDD1894  [13]
C362
 Probe Info  		27.47  LDD2023  [13]
C382
 Probe Info  		9.71  LDD2041  [13]
C388
 Probe Info  		39.12  LDD2047  [13]
C390
 Probe Info  		21.56  LDD2049  [13]
C403
 Probe Info  		14.22  LDD2061  [13]
FFF probe11
 Probe Info  		20.00  LDD0472  [14]
FFF probe13
 Probe Info  		20.00  LDD0475  [14]
FFF probe14
 Probe Info  		20.00  LDD0477  [14]
FFF probe3
 Probe Info  		20.00  LDD0464  [14]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C93(0.94)  LDD1507  [9]
 LDCM0215  AC10 HEK-293T C93(0.94)  LDD1508  [9]
 LDCM0226  AC11 HEK-293T C93(0.75)  LDD1509  [9]
 LDCM0237  AC12 HEK-293T C93(1.00)  LDD1510  [9]
 LDCM0259  AC14 HEK-293T C93(1.17)  LDD1512  [9]
 LDCM0270  AC15 HEK-293T C93(0.97)  LDD1513  [9]
 LDCM0276  AC17 HEK-293T C93(1.09)  LDD1515  [9]
 LDCM0277  AC18 HEK-293T C93(0.76)  LDD1516  [9]
 LDCM0278  AC19 HEK-293T C93(0.67)  LDD1517  [9]
 LDCM0279  AC2 HEK-293T C93(0.80)  LDD1518  [9]
 LDCM0280  AC20 HEK-293T C93(0.93)  LDD1519  [9]
 LDCM0281  AC21 HEK-293T C93(0.79)  LDD1520  [9]
 LDCM0282  AC22 HEK-293T C93(1.08)  LDD1521  [9]
 LDCM0283  AC23 HEK-293T C93(0.99)  LDD1522  [9]
 LDCM0284  AC24 HEK-293T C93(1.29)  LDD1523  [9]
 LDCM0285  AC25 HEK-293T C93(0.84)  LDD1524  [9]
 LDCM0286  AC26 HEK-293T C93(0.82)  LDD1525  [9]
 LDCM0287  AC27 HEK-293T C93(0.68)  LDD1526  [9]
 LDCM0288  AC28 HEK-293T C93(0.85)  LDD1527  [9]
 LDCM0289  AC29 HEK-293T C93(0.78)  LDD1528  [9]
 LDCM0290  AC3 HEK-293T C93(0.65)  LDD1529  [9]
 LDCM0291  AC30 HEK-293T C93(1.01)  LDD1530  [9]
 LDCM0292  AC31 HEK-293T C93(0.87)  LDD1531  [9]
 LDCM0293  AC32 HEK-293T C93(0.94)  LDD1532  [9]
 LDCM0294  AC33 HEK-293T C93(0.95)  LDD1533  [9]
 LDCM0295  AC34 HEK-293T C93(0.81)  LDD1534  [9]
 LDCM0296  AC35 HEK-293T C93(0.68)  LDD1535  [9]
 LDCM0297  AC36 HEK-293T C93(0.84)  LDD1536  [9]
 LDCM0298  AC37 HEK-293T C93(0.96)  LDD1537  [9]
 LDCM0299  AC38 HEK-293T C93(1.16)  LDD1538  [9]
 LDCM0300  AC39 HEK-293T C93(1.01)  LDD1539  [9]
 LDCM0301  AC4 HEK-293T C93(0.90)  LDD1540  [9]
 LDCM0302  AC40 HEK-293T C93(1.08)  LDD1541  [9]
 LDCM0303  AC41 HEK-293T C93(1.10)  LDD1542  [9]
 LDCM0304  AC42 HEK-293T C93(0.80)  LDD1543  [9]
 LDCM0305  AC43 HEK-293T C93(0.79)  LDD1544  [9]
 LDCM0306  AC44 HEK-293T C93(0.99)  LDD1545  [9]
 LDCM0307  AC45 HEK-293T C93(0.92)  LDD1546  [9]
 LDCM0308  AC46 HEK-293T C93(1.17)  LDD1547  [9]
 LDCM0309  AC47 HEK-293T C93(0.97)  LDD1548  [9]
 LDCM0310  AC48 HEK-293T C93(1.30)  LDD1549  [9]
 LDCM0311  AC49 HEK-293T C93(0.97)  LDD1550  [9]
 LDCM0312  AC5 HEK-293T C93(0.81)  LDD1551  [9]
 LDCM0313  AC50 HEK-293T C93(0.95)  LDD1552  [9]
 LDCM0314  AC51 HEK-293T C93(0.77)  LDD1553  [9]
 LDCM0315  AC52 HEK-293T C93(1.02)  LDD1554  [9]
 LDCM0316  AC53 HEK-293T C93(0.86)  LDD1555  [9]
 LDCM0317  AC54 HEK-293T C93(1.16)  LDD1556  [9]
 LDCM0318  AC55 HEK-293T C93(1.00)  LDD1557  [9]
 LDCM0319  AC56 HEK-293T C93(1.06)  LDD1558  [9]
 LDCM0320  AC57 HEK-293T C93(0.92)  LDD1559  [9]
 LDCM0321  AC58 HEK-293T C93(0.96)  LDD1560  [9]
 LDCM0322  AC59 HEK-293T C93(0.96)  LDD1561  [9]
 LDCM0323  AC6 HEK-293T C93(0.96)  LDD1562  [9]
 LDCM0324  AC60 HEK-293T C93(0.99)  LDD1563  [9]
 LDCM0325  AC61 HEK-293T C93(0.90)  LDD1564  [9]
 LDCM0326  AC62 HEK-293T C93(0.98)  LDD1565  [9]
 LDCM0327  AC63 HEK-293T C93(0.79)  LDD1566  [9]
 LDCM0328  AC64 HEK-293T C93(0.98)  LDD1567  [9]
 LDCM0334  AC7 HEK-293T C93(0.83)  LDD1568  [9]
 LDCM0345  AC8 HEK-293T C93(1.01)  LDD1569  [9]
 LDCM0248  AKOS034007472 HEK-293T C93(0.85)  LDD1511  [9]
 LDCM0356  AKOS034007680 HEK-293T C93(0.93)  LDD1570  [9]
 LDCM0275  AKOS034007705 HEK-293T C93(1.26)  LDD1514  [9]
 LDCM0156  Aniline NCI-H1299 9.90  LDD0403  [1]
 LDCM0367  CL1 HEK-293T C93(1.03)  LDD1571  [9]
 LDCM0368  CL10 HEK-293T C93(1.71)  LDD1572  [9]
 LDCM0370  CL101 HEK-293T C93(0.99)  LDD1574  [9]
 LDCM0371  CL102 HEK-293T C93(1.14)  LDD1575  [9]
 LDCM0372  CL103 HEK-293T C93(1.16)  LDD1576  [9]
 LDCM0374  CL105 HEK-293T C93(0.90)  LDD1578  [9]
 LDCM0375  CL106 HEK-293T C93(1.14)  LDD1579  [9]
 LDCM0376  CL107 HEK-293T C93(1.13)  LDD1580  [9]
 LDCM0378  CL109 HEK-293T C93(1.04)  LDD1582  [9]
 LDCM0379  CL11 HEK-293T C93(0.94)  LDD1583  [9]
 LDCM0380  CL110 HEK-293T C93(1.24)  LDD1584  [9]
 LDCM0381  CL111 HEK-293T C93(1.09)  LDD1585  [9]
 LDCM0383  CL113 HEK-293T C93(0.97)  LDD1587  [9]
 LDCM0384  CL114 HEK-293T C93(1.09)  LDD1588  [9]
 LDCM0385  CL115 HEK-293T C93(1.04)  LDD1589  [9]
 LDCM0387  CL117 HEK-293T C93(0.90)  LDD1591  [9]
 LDCM0388  CL118 HEK-293T C93(1.09)  LDD1592  [9]
 LDCM0389  CL119 HEK-293T C93(1.04)  LDD1593  [9]
 LDCM0390  CL12 HEK-293T C93(1.07)  LDD1594  [9]
 LDCM0392  CL121 HEK-293T C93(0.93)  LDD1596  [9]
 LDCM0393  CL122 HEK-293T C93(1.15)  LDD1597  [9]
 LDCM0394  CL123 HEK-293T C93(1.33)  LDD1598  [9]
 LDCM0396  CL125 HEK-293T C93(0.82)  LDD1600  [9]
 LDCM0397  CL126 HEK-293T C93(0.98)  LDD1601  [9]
 LDCM0398  CL127 HEK-293T C93(0.88)  LDD1602  [9]
 LDCM0400  CL13 HEK-293T C93(1.00)  LDD1604  [9]
 LDCM0401  CL14 HEK-293T C93(0.98)  LDD1605  [9]
 LDCM0402  CL15 HEK-293T C93(1.07)  LDD1606  [9]
 LDCM0404  CL17 HEK-293T C93(1.00)  LDD1608  [9]
 LDCM0405  CL18 HEK-293T C93(0.86)  LDD1609  [9]
 LDCM0406  CL19 HEK-293T C93(0.79)  LDD1610  [9]
 LDCM0407  CL2 HEK-293T C93(0.90)  LDD1611  [9]
 LDCM0408  CL20 HEK-293T C93(0.98)  LDD1612  [9]
 LDCM0409  CL21 HEK-293T C93(0.82)  LDD1613  [9]
 LDCM0410  CL22 HEK-293T C93(1.26)  LDD1614  [9]
 LDCM0411  CL23 HEK-293T C93(0.92)  LDD1615  [9]
 LDCM0412  CL24 HEK-293T C93(1.13)  LDD1616  [9]
 LDCM0413  CL25 HEK-293T C93(1.13)  LDD1617  [9]
 LDCM0414  CL26 HEK-293T C93(1.00)  LDD1618  [9]
 LDCM0415  CL27 HEK-293T C93(1.03)  LDD1619  [9]
 LDCM0417  CL29 HEK-293T C93(1.02)  LDD1621  [9]
 LDCM0418  CL3 HEK-293T C93(0.93)  LDD1622  [9]
 LDCM0419  CL30 HEK-293T C93(0.84)  LDD1623  [9]
 LDCM0420  CL31 HEK-293T C93(0.69)  LDD1624  [9]
 LDCM0421  CL32 HEK-293T C93(0.96)  LDD1625  [9]
 LDCM0422  CL33 HEK-293T C93(1.58)  LDD1626  [9]
 LDCM0423  CL34 HEK-293T C93(0.98)  LDD1627  [9]
 LDCM0424  CL35 HEK-293T C93(0.93)  LDD1628  [9]
 LDCM0425  CL36 HEK-293T C93(1.12)  LDD1629  [9]
 LDCM0426  CL37 HEK-293T C93(0.87)  LDD1630  [9]
 LDCM0428  CL39 HEK-293T C93(0.88)  LDD1632  [9]
 LDCM0431  CL41 HEK-293T C93(0.96)  LDD1635  [9]
 LDCM0432  CL42 HEK-293T C93(0.76)  LDD1636  [9]
 LDCM0433  CL43 HEK-293T C93(0.68)  LDD1637  [9]
 LDCM0434  CL44 HEK-293T C93(0.85)  LDD1638  [9]
 LDCM0435  CL45 HEK-293T C93(0.92)  LDD1639  [9]
 LDCM0436  CL46 HEK-293T C93(0.96)  LDD1640  [9]
 LDCM0437  CL47 HEK-293T C93(0.83)  LDD1641  [9]
 LDCM0438  CL48 HEK-293T C93(1.16)  LDD1642  [9]
 LDCM0439  CL49 HEK-293T C93(1.09)  LDD1643  [9]
 LDCM0440  CL5 HEK-293T C93(0.92)  LDD1644  [9]
 LDCM0441  CL50 HEK-293T C93(1.10)  LDD1645  [9]
 LDCM0444  CL53 HEK-293T C93(1.05)  LDD1647  [9]
 LDCM0445  CL54 HEK-293T C93(1.32)  LDD1648  [9]
 LDCM0446  CL55 HEK-293T C93(0.74)  LDD1649  [9]
 LDCM0447  CL56 HEK-293T C93(0.95)  LDD1650  [9]
 LDCM0448  CL57 HEK-293T C93(0.77)  LDD1651  [9]
 LDCM0449  CL58 HEK-293T C93(1.27)  LDD1652  [9]
 LDCM0450  CL59 HEK-293T C93(0.94)  LDD1653  [9]
 LDCM0451  CL6 HEK-293T C93(0.81)  LDD1654  [9]
 LDCM0452  CL60 HEK-293T C93(1.13)  LDD1655  [9]
 LDCM0453  CL61 HEK-293T C93(0.83)  LDD1656  [9]
 LDCM0454  CL62 HEK-293T C93(1.11)  LDD1657  [9]
 LDCM0455  CL63 HEK-293T C93(0.96)  LDD1658  [9]
 LDCM0457  CL65 HEK-293T C93(1.03)  LDD1660  [9]
 LDCM0458  CL66 HEK-293T C93(0.71)  LDD1661  [9]
 LDCM0459  CL67 HEK-293T C93(0.65)  LDD1662  [9]
 LDCM0460  CL68 HEK-293T C93(0.91)  LDD1663  [9]
 LDCM0461  CL69 HEK-293T C93(0.84)  LDD1664  [9]
 LDCM0462  CL7 HEK-293T C93(0.64)  LDD1665  [9]
 LDCM0463  CL70 HEK-293T C93(1.03)  LDD1666  [9]
 LDCM0464  CL71 HEK-293T C93(0.91)  LDD1667  [9]
 LDCM0465  CL72 HEK-293T C93(1.00)  LDD1668  [9]
 LDCM0466  CL73 HEK-293T C93(0.76)  LDD1669  [9]
 LDCM0467  CL74 HEK-293T C93(0.99)  LDD1670  [9]
 LDCM0470  CL77 HEK-293T C93(1.03)  LDD1673  [9]
 LDCM0471  CL78 HEK-293T C93(0.89)  LDD1674  [9]
 LDCM0472  CL79 HEK-293T C93(0.74)  LDD1675  [9]
 LDCM0473  CL8 HEK-293T C93(3.21)  LDD1676  [9]
 LDCM0474  CL80 HEK-293T C93(0.93)  LDD1677  [9]
 LDCM0475  CL81 HEK-293T C93(0.94)  LDD1678  [9]
 LDCM0476  CL82 HEK-293T C93(1.15)  LDD1679  [9]
 LDCM0477  CL83 HEK-293T C93(0.88)  LDD1680  [9]
 LDCM0478  CL84 HEK-293T C93(1.12)  LDD1681  [9]
 LDCM0479  CL85 HEK-293T C93(0.81)  LDD1682  [9]
 LDCM0480  CL86 HEK-293T C93(0.87)  LDD1683  [9]
 LDCM0481  CL87 HEK-293T C93(0.84)  LDD1684  [9]
 LDCM0483  CL89 HEK-293T C93(0.81)  LDD1686  [9]
 LDCM0484  CL9 HEK-293T C93(0.72)  LDD1687  [9]
 LDCM0485  CL90 HEK-293T C93(1.95)  LDD1688  [9]
 LDCM0486  CL91 HEK-293T C93(0.64)  LDD1689  [9]
 LDCM0487  CL92 HEK-293T C93(0.88)  LDD1690  [9]
 LDCM0488  CL93 HEK-293T C93(0.90)  LDD1691  [9]
 LDCM0489  CL94 HEK-293T C93(0.99)  LDD1692  [9]
 LDCM0490  CL95 HEK-293T C93(0.98)  LDD1693  [9]
 LDCM0491  CL96 HEK-293T C93(0.92)  LDD1694  [9]
 LDCM0492  CL97 HEK-293T C93(0.90)  LDD1695  [9]
 LDCM0493  CL98 HEK-293T C93(1.04)  LDD1696  [9]
 LDCM0494  CL99 HEK-293T C93(0.94)  LDD1697  [9]
 LDCM0495  E2913 HEK-293T C93(1.00)  LDD1698  [9]
 LDCM0468  Fragment33 HEK-293T C93(1.02)  LDD1671  [9]
 LDCM0427  Fragment51 HEK-293T C93(0.98)  LDD1631  [9]
 LDCM0131  RA190 MM1.R 2.64  LDD0299  [8]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Nadh Small molecular drug DB00157

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Chemoproteomics and Phosphoproteomics Profiling Reveals Salvianolic Acid A as a Covalent Inhibitor of mTORC1. J Proteome Res. 2023 Jul 7;22(7):2450-2459. doi: 10.1021/acs.jproteome.3c00188. Epub 2023 Jun 22.
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
7 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
8 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
10 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
11 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
12 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
13 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
14 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.