Details of the Target

General Information of Target

Target ID LDTP10046
Target Name Endoplasmic reticulum-Golgi intermediate compartment protein 1 (ERGIC1)
Gene Name ERGIC1
Gene ID 57222
Synonyms
ERGIC32; KIAA1181; Endoplasmic reticulum-Golgi intermediate compartment protein 1; ER-Golgi intermediate compartment 32 kDa protein; ERGIC-32
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MACSRPPSQCEPTSLPPGPPAGRRHLPLSRRRREMSSNKEQRSAVFVILFALITILILYS
SNSANEVFHYGSLRGRSRRPVNLKKWSITDGYVPILGNKTLPSRCHQCVIVSSSSHLLGT
KLGPEIERAECTIRMNDAPTTGYSADVGNKTTYRVVAHSSVFRVLRRPQEFVNRTPETVF
IFWGPPSKMQKPQGSLVRVIQRAGLVFPNMEAYAVSPGRMRQFDDLFRGETGKDREKSHS
WLSTGWFTMVIAVELCDHVHVYGMVPPNYCSQRPRLQRMPYHYYEPKGPDECVTYIQNEH
SRKGNHHRFITEKRVFSSWAQLYGITFSHPSWT
Target Bioclass
Other
Family
ERGIC family
Subcellular location
Endoplasmic reticulum membrane
Function Possible role in transport between endoplasmic reticulum and Golgi.
Uniprot ID
Q969X5
Ensemble ID
ENST00000393784.8
HGNC ID
HGNC:29205

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.S272P DBIA    Probe Info 
IGROV1 Deletion: p.G173EfsTer17 DBIA    Probe Info 
NCIH1155 SNV: p.C82Y .
SKMEL5 SNV: p.Q285Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		2.27  LDD0318  [1]
TH211
 Probe Info  		Y219(20.00); Y196(16.04)  LDD0260  [2]
YN-1
 Probe Info  		100.00  LDD0444  [3]
STPyne
 Probe Info  		K190(10.00); K288(6.14)  LDD0277  [4]
DBIA
 Probe Info  		C70(3.37)  LDD3310  [5]
BTD
 Probe Info  		C115(0.68)  LDD2117  [6]
IPM
 Probe Info  		C115(1.83)  LDD1702  [6]
5E-2FA
 Probe Info  		N.A.  LDD2235  [7]
m-APA
 Probe Info  		H221(0.00); H165(0.00)  LDD2231  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [8]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [8]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [8]
Acrolein
 Probe Info  		N.A.  LDD0217  [9]
Crotonaldehyde
 Probe Info  		H221(0.00); C115(0.00)  LDD0219  [9]
AOyne
 Probe Info  		15.00  LDD0443  [10]
MPP-AC
 Probe Info  		N.A.  LDD0428  [11]
NAIA_5
 Probe Info  		N.A.  LDD2223  [12]
TER-AC
 Probe Info  		N.A.  LDD0426  [11]
TPP-AC
 Probe Info  		N.A.  LDD0427  [11]
PAL-AfBPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C091
 Probe Info  		10.70  LDD1782  [13]
C092
 Probe Info  		22.94  LDD1783  [13]
C094
 Probe Info  		33.36  LDD1785  [13]
C141
 Probe Info  		9.13  LDD1823  [13]
C210
 Probe Info  		67.65  LDD1884  [13]
C213
 Probe Info  		17.27  LDD1887  [13]
C228
 Probe Info  		14.62  LDD1901  [13]
C246
 Probe Info  		12.30  LDD1919  [13]
C289
 Probe Info  		38.05  LDD1959  [13]
C362
 Probe Info  		52.71  LDD2023  [13]
C363
 Probe Info  		19.84  LDD2024  [13]
C364
 Probe Info  		19.16  LDD2025  [13]
C366
 Probe Info  		5.62  LDD2027  [13]
C388
 Probe Info  		38.32  LDD2047  [13]
C403
 Probe Info  		14.93  LDD2061  [13]
STS-1
 Probe Info  		N.A.  LDD0136  [14]
STS-2
 Probe Info  		N.A.  LDD0138  [14]
HM-2
 Probe Info  		1.97  LDD0436  [15]
OEA-DA
 Probe Info  		12.51  LDD0046  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C115(0.68)  LDD2117  [6]
 LDCM0108  Chloroacetamide HeLa H96(0.00); H165(0.00)  LDD0222  [9]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C115(1.83)  LDD1702  [6]
 LDCM0173  HM30181 Hep-G2 1.97  LDD0436  [15]
 LDCM0107  IAA HeLa H165(0.00); H96(0.00)  LDD0221  [9]
 LDCM0022  KB02 697 C70(1.78)  LDD2245  [5]
 LDCM0023  KB03 697 C70(2.28)  LDD2662  [5]
 LDCM0024  KB05 COLO792 C70(3.37)  LDD3310  [5]
 LDCM0109  NEM HeLa H165(0.00); H96(0.00); H100(0.00); H185(0.00)  LDD0223  [9]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C115(0.77)  LDD2123  [6]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C115(0.49)  LDD2125  [6]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C115(0.39)  LDD2127  [6]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C115(1.08)  LDD2136  [6]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C115(0.34)  LDD2140  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Peroxisomal bifunctional enzyme (EHHADH) Enoyl-CoA hydratase/isomerase family; 3-hydroxyacyl-CoA dehydrogenase family Q08426
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nucleoporin p58/p45 (NUP58) NUP58 family Q9BVL2
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Krueppel-like factor 11 (KLF11) Sp1 C2H2-type zinc-finger protein family O14901
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) ERGIC family Q9Y282

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
4 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
12 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
13 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
14 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
15 Affinity-Based Protein Profiling Reveals Cellular Targets of Photoreactive Anticancer Inhibitors. ACS Chem Biol. 2019 Dec 20;14(12):2546-2552. doi: 10.1021/acschembio.9b00784. Epub 2019 Nov 26.
16 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570