Details of the Target

General Information of Target

Target ID LDTP01986
Target Name NADH-ubiquinone oxidoreductase chain 5 (MT-ND5)
Gene Name MT-ND5
Gene ID 4540
Synonyms
MTND5; NADH5; ND5; NADH-ubiquinone oxidoreductase chain 5; EC 7.1.1.2; NADH dehydrogenase subunit 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTMHTTMTTLTLTSLIPPILTTLVNPNKKNSYPHYVKSIVASTFIISLFPTTMFMCLDQE
VIISNWHWATTQTTQLSLSFKLDYFSMMFIPVALFVTWSIMEFSLWYMNSDPNINQFFKY
LLIFLITMLILVTANNLFQLFIGWEGVGIMSFLLISWWYARADANTAAIQAILYNRIGDI
GFILALAWFILHSNSWDPQQMALLNANPSLTPLLGLLLAAAGKSAQLGLHPWLPSAMEGP
TPVSALLHSSTMVVAGIFLLIRFHPLAENSPLIQTLTLCLGAITTLFAAVCALTQNDIKK
IVAFSTSSQLGLMMVTIGINQPHLAFLHICTHAFFKAMLFMCSGSIIHNLNNEQDIRKMG
GLLKTMPLTSTSLTIGSLALAGMPFLTGFYSKDHIIETANMSYTNAWALSITLIATSLTS
AYSTRMILLTLTGQPRFPTLTNINENNPTLLNPIKRLAAGSLFAGFLITNNISPASPFQT
TIPLYLKLTALAVTFLGLLTALDLNYLTNKLKMKSPLCTFYFSNMLGFYPSITHRTIPYL
GLLTSQNLPLLLLDLTWLEKLLPKTISQHQISTSIITSTQKGMIKLYFLSFFFPLILTLL
LIT
Target Bioclass
Enzyme
Family
Complex I subunit 5 family
Subcellular location
Mitochondrion inner membrane
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Uniprot ID
P03915
Ensemble ID
ENST00000361567.2
HGNC ID
HGNC:7461
ChEMBL ID
CHEMBL2363065

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Jackson_1
 Probe Info  		10.99  LDD0122  [1]
YN-4
 Probe Info  		100.00  LDD0445  [2]
IPM
 Probe Info  		N.A.  LDD0241  [3]
Johansson_61
 Probe Info  		_(20.00)  LDD1487  [4]
YY4-yne
 Probe Info  		3.26  LDD0400  [5]
AHL-Pu-1
 Probe Info  		C518(2.65)  LDD0170  [6]
DBIA
 Probe Info  		C518(1.75)  LDD1095  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [8]
NAIA_5
 Probe Info  		N.A.  LDD2224  [9]
STPyne
 Probe Info  		N.A.  LDD0009  [10]
TPP-AC
 Probe Info  		N.A.  LDD0427  [11]
PAL-AfBPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C091
 Probe Info  		13.64  LDD1782  [12]
C092
 Probe Info  		25.28  LDD1783  [12]
C094
 Probe Info  		43.11  LDD1785  [12]
C112
 Probe Info  		17.63  LDD1799  [12]
C429
 Probe Info  		11.88  LDD2084  [12]
C431
 Probe Info  		14.83  LDD2086  [12]
FFF probe11
 Probe Info  		20.00  LDD0471  [13]
FFF probe13
 Probe Info  		18.55  LDD0475  [13]
FFF probe14
 Probe Info  		14.50  LDD0477  [13]
FFF probe3
 Probe Info  		12.25  LDD0464  [13]
JN0003
 Probe Info  		20.00  LDD0469  [13]
STS-2
 Probe Info  		N.A.  LDD0138  [14]
VE-P
 Probe Info  		N.A.  LDD0396  [15]
OEA-DA
 Probe Info  		5.60  LDD0046  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C518(2.65)  LDD0170  [6]
 LDCM0026  4SU-RNA+native RNA DM93 C518(8.43)  LDD0171  [6]
 LDCM0216  AC100 PaTu 8988t C518(1.75)  LDD1095  [7]
 LDCM0217  AC101 PaTu 8988t C518(2.01)  LDD1096  [7]
 LDCM0218  AC102 PaTu 8988t C518(1.08)  LDD1097  [7]
 LDCM0219  AC103 PaTu 8988t C518(1.06)  LDD1098  [7]
 LDCM0220  AC104 PaTu 8988t C518(0.85)  LDD1099  [7]
 LDCM0221  AC105 PaTu 8988t C518(1.31)  LDD1100  [7]
 LDCM0222  AC106 PaTu 8988t C518(1.22)  LDD1101  [7]
 LDCM0223  AC107 PaTu 8988t C518(1.32)  LDD1102  [7]
 LDCM0224  AC108 PaTu 8988t C518(1.48)  LDD1103  [7]
 LDCM0225  AC109 PaTu 8988t C518(1.95)  LDD1104  [7]
 LDCM0227  AC110 PaTu 8988t C518(1.52)  LDD1106  [7]
 LDCM0228  AC111 PaTu 8988t C518(1.17)  LDD1107  [7]
 LDCM0229  AC112 PaTu 8988t C518(1.91)  LDD1108  [7]
 LDCM0230  AC113 PaTu 8988t C518(0.73)  LDD1109  [7]
 LDCM0231  AC114 PaTu 8988t C518(0.84)  LDD1110  [7]
 LDCM0232  AC115 PaTu 8988t C518(0.76)  LDD1111  [7]
 LDCM0233  AC116 PaTu 8988t C518(0.99)  LDD1112  [7]
 LDCM0234  AC117 PaTu 8988t C518(0.91)  LDD1113  [7]
 LDCM0235  AC118 PaTu 8988t C518(1.23)  LDD1114  [7]
 LDCM0236  AC119 PaTu 8988t C518(0.89)  LDD1115  [7]
 LDCM0238  AC120 PaTu 8988t C518(0.94)  LDD1117  [7]
 LDCM0239  AC121 PaTu 8988t C518(0.86)  LDD1118  [7]
 LDCM0240  AC122 PaTu 8988t C518(0.80)  LDD1119  [7]
 LDCM0241  AC123 PaTu 8988t C518(1.19)  LDD1120  [7]
 LDCM0242  AC124 PaTu 8988t C518(1.38)  LDD1121  [7]
 LDCM0243  AC125 PaTu 8988t C518(1.53)  LDD1122  [7]
 LDCM0244  AC126 PaTu 8988t C518(1.32)  LDD1123  [7]
 LDCM0245  AC127 PaTu 8988t C518(1.20)  LDD1124  [7]
 LDCM0246  AC128 PaTu 8988t C518(0.57)  LDD1125  [7]
 LDCM0247  AC129 PaTu 8988t C518(0.54)  LDD1126  [7]
 LDCM0249  AC130 PaTu 8988t C518(0.52)  LDD1128  [7]
 LDCM0250  AC131 PaTu 8988t C518(1.77)  LDD1129  [7]
 LDCM0251  AC132 PaTu 8988t C518(0.76)  LDD1130  [7]
 LDCM0252  AC133 PaTu 8988t C518(0.83)  LDD1131  [7]
 LDCM0253  AC134 PaTu 8988t C518(0.92)  LDD1132  [7]
 LDCM0254  AC135 PaTu 8988t C518(0.86)  LDD1133  [7]
 LDCM0255  AC136 PaTu 8988t C518(0.49)  LDD1134  [7]
 LDCM0256  AC137 PaTu 8988t C518(0.57)  LDD1135  [7]
 LDCM0257  AC138 PaTu 8988t C518(0.48)  LDD1136  [7]
 LDCM0258  AC139 PaTu 8988t C518(0.48)  LDD1137  [7]
 LDCM0260  AC140 PaTu 8988t C518(0.72)  LDD1139  [7]
 LDCM0261  AC141 PaTu 8988t C518(0.69)  LDD1140  [7]
 LDCM0262  AC142 PaTu 8988t C518(0.66)  LDD1141  [7]
 LDCM0285  AC25 PaTu 8988t C518(0.66)  LDD1164  [7]
 LDCM0286  AC26 PaTu 8988t C518(0.72)  LDD1165  [7]
 LDCM0287  AC27 PaTu 8988t C518(0.95)  LDD1166  [7]
 LDCM0288  AC28 PaTu 8988t C518(1.27)  LDD1167  [7]
 LDCM0289  AC29 PaTu 8988t C518(0.65)  LDD1168  [7]
 LDCM0291  AC30 PaTu 8988t C518(0.65)  LDD1170  [7]
 LDCM0292  AC31 PaTu 8988t C518(0.97)  LDD1171  [7]
 LDCM0293  AC32 PaTu 8988t C518(1.02)  LDD1172  [7]
 LDCM0294  AC33 PaTu 8988t C518(1.87)  LDD1173  [7]
 LDCM0295  AC34 PaTu 8988t C518(0.69)  LDD1174  [7]
 LDCM0308  AC46 PaTu 8988t C518(1.20)  LDD1187  [7]
 LDCM0309  AC47 PaTu 8988t C518(1.02)  LDD1188  [7]
 LDCM0310  AC48 PaTu 8988t C518(1.01)  LDD1189  [7]
 LDCM0311  AC49 PaTu 8988t C518(1.39)  LDD1190  [7]
 LDCM0313  AC50 PaTu 8988t C518(1.63)  LDD1192  [7]
 LDCM0314  AC51 PaTu 8988t C518(1.47)  LDD1193  [7]
 LDCM0315  AC52 PaTu 8988t C518(1.59)  LDD1194  [7]
 LDCM0316  AC53 PaTu 8988t C518(1.57)  LDD1195  [7]
 LDCM0317  AC54 PaTu 8988t C518(1.84)  LDD1196  [7]
 LDCM0318  AC55 PaTu 8988t C518(1.01)  LDD1197  [7]
 LDCM0319  AC56 PaTu 8988t C518(2.33)  LDD1198  [7]
 LDCM0349  AC83 PaTu 8988t C518(1.16)  LDD1228  [7]
 LDCM0350  AC84 PaTu 8988t C518(1.14)  LDD1229  [7]
 LDCM0351  AC85 PaTu 8988t C518(1.08)  LDD1230  [7]
 LDCM0352  AC86 PaTu 8988t C518(1.21)  LDD1231  [7]
 LDCM0353  AC87 PaTu 8988t C518(0.94)  LDD1232  [7]
 LDCM0354  AC88 PaTu 8988t C518(1.15)  LDD1233  [7]
 LDCM0355  AC89 PaTu 8988t C518(1.44)  LDD1234  [7]
 LDCM0357  AC90 PaTu 8988t C518(1.02)  LDD1236  [7]
 LDCM0358  AC91 PaTu 8988t C518(0.98)  LDD1237  [7]
 LDCM0359  AC92 PaTu 8988t C518(1.12)  LDD1238  [7]
 LDCM0360  AC93 PaTu 8988t C518(1.16)  LDD1239  [7]
 LDCM0361  AC94 PaTu 8988t C518(1.11)  LDD1240  [7]
 LDCM0362  AC95 PaTu 8988t C518(1.53)  LDD1241  [7]
 LDCM0363  AC96 PaTu 8988t C518(0.76)  LDD1242  [7]
 LDCM0364  AC97 PaTu 8988t C518(1.82)  LDD1243  [7]
 LDCM0365  AC98 PaTu 8988t C518(1.81)  LDD1244  [7]
 LDCM0366  AC99 PaTu 8988t C518(1.28)  LDD1245  [7]
 LDCM0382  CL112 PaTu 8988t C518(1.38)  LDD1261  [7]
 LDCM0383  CL113 PaTu 8988t C518(0.78)  LDD1262  [7]
 LDCM0384  CL114 PaTu 8988t C518(2.62)  LDD1263  [7]
 LDCM0385  CL115 PaTu 8988t C518(1.15)  LDD1264  [7]
 LDCM0386  CL116 PaTu 8988t C518(1.00)  LDD1265  [7]
 LDCM0392  CL121 PaTu 8988t C518(1.19)  LDD1271  [7]
 LDCM0393  CL122 PaTu 8988t C518(4.45)  LDD1272  [7]
 LDCM0394  CL123 PaTu 8988t C518(10.40)  LDD1273  [7]
 LDCM0395  CL124 PaTu 8988t C518(3.34)  LDD1274  [7]
 LDCM0469  CL76 PaTu 8988t C518(1.00)  LDD1348  [7]
 LDCM0470  CL77 PaTu 8988t C518(4.01)  LDD1349  [7]
 LDCM0471  CL78 PaTu 8988t C518(1.32)  LDD1350  [7]
 LDCM0472  CL79 PaTu 8988t C518(1.29)  LDD1351  [7]
 LDCM0474  CL80 PaTu 8988t C518(1.65)  LDD1353  [7]
 LDCM0475  CL81 PaTu 8988t C518(1.83)  LDD1354  [7]
 LDCM0476  CL82 PaTu 8988t C518(0.98)  LDD1355  [7]
 LDCM0477  CL83 PaTu 8988t C518(1.59)  LDD1356  [7]
 LDCM0478  CL84 PaTu 8988t C518(1.17)  LDD1357  [7]
 LDCM0479  CL85 PaTu 8988t C518(1.89)  LDD1358  [7]
 LDCM0480  CL86 PaTu 8988t C518(1.00)  LDD1359  [7]
 LDCM0481  CL87 PaTu 8988t C518(1.89)  LDD1360  [7]
 LDCM0482  CL88 PaTu 8988t C518(1.47)  LDD1361  [7]
 LDCM0483  CL89 PaTu 8988t C518(1.57)  LDD1362  [7]
 LDCM0485  CL90 PaTu 8988t C518(1.85)  LDD1364  [7]
 LDCM0191  Compound 21 HEK-293T 6.62  LDD0508  [13]
 LDCM0192  Compound 35 HEK-293T 3.52  LDD0509  [13]
 LDCM0193  Compound 36 HEK-293T 5.89  LDD0511  [13]
 LDCM0615  Fragment63-R Jurkat _(20.00)  LDD1487  [4]
 LDCM0154  YY4 T cell 3.26  LDD0400  [5]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Nadh Small molecular drug DB00157

References

1 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
5 A Chemical Proteomic Probe for the Mitochondrial Pyruvate Carrier Complex. Angew Chem Int Ed Engl. 2020 Mar 2;59(10):3896-3899. doi: 10.1002/anie.201914391. Epub 2020 Feb 11.
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
8 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
12 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
13 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
14 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
15 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
16 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570