Details of the Target

General Information of Target

Target ID LDTP14105
Target Name YTH domain-containing family protein 2 (YTHDF2)
Gene Name YTHDF2
Gene ID 51441
Synonyms
HGRG8; YTH domain-containing family protein 2; DF2; CLL-associated antigen KW-14; High-glucose-regulated protein 8; Renal carcinoma antigen NY-REN-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAQKKYLQAKLTQFLREDRIQLWKPPYTDENKKVGLALKDLAKQYSDRLECCENEVEKVI
EEIRCKAIERGTGNDNYRTTGIATIEVFLPPRLKKDRKNLLETRLHITGRELRSKIAETF
GLQENYIKIVINKKQLQLGKTLEEQGVAHNVKAMVLELKQSEEDARKNFQLEEEEQNEAK
LKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRIPPSERKALMLAMGYH
EKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDTVDNYAVLQLDIVWCYFRLEQLECL
DDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGIRNYHSGNDVEAY
EYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLGLRACDGNVDHAATHITNRREELAQ
IRKEEKEKKRRRLENIRFLKGMGYSTHAAQQVLHAASGNLDEALKILLSNPQMWWLNDSN
PETDNRQESPSQENIDRLVYMGFDALVAEAALRVFRGNVQLAAQTLAHNGGSLPPELPLS
PEDSLSPPATSPSDSAGTSSASTDEDMETEAVNEILEDIPEHEEDYLDSTLEDEEIIIAE
YLSYVENRKSATKKN
Target Bioclass
Other
Family
YTHDF family, YTHDF2 subfamily
Subcellular location
Cytoplasm, cytosol
Function
Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development. Also required during spermatogenesis: regulates spermagonial adhesion by promoting degradation of m6A-containing transcripts coding for matrix metallopeptidases. Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation. Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets. Inhibits neural specification of induced pluripotent stem cells by binding to methylated neural-specific mRNAs and promoting their degradation, thereby restraining neural differentiation. Regulates circadian regulation of hepatic lipid metabolism: acts by promoting m6A-dependent degradation of PPARA transcripts. Regulates the innate immune response to infection by inhibiting the type I interferon response: acts by binding to m6A-containing IFNB transcripts and promoting their degradation. May also act as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. Regulates mitotic entry by promoting the phase-specific m6A-dependent degradation of WEE1 transcripts. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation. The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules. May also recognize and bind RNAs modified by C5-methylcytosine (m5C) and act as a regulator of rRNA processing.; (Microbial infection) Promotes viral gene expression and replication of polyomavirus SV40: acts by binding to N6-methyladenosine (m6A)-containing viral RNAs.; (Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK.219 and iSLK.BAC16 cells). Acts by binding to N6-methyladenosine (m6A)-containing viral RNAs.
Uniprot ID
Q9Y5A9
Ensemble ID
ENST00000373812.8
HGNC ID
HGNC:31675
ChEMBL ID
CHEMBL4295992

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JURKAT SNV: p.E420K Compound 10    Probe Info 
LNCaP clone FGC SNV: p.A318V DBIA    Probe Info 
MDST8 SNV: p.R447C DBIA    Probe Info 
MEC1 SNV: p.K571N DBIA    Probe Info 
U2OS SNV: p.P101R DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 27 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
A-EBA
 Probe Info  		3.67  LDD0215  [2]
CY-1
 Probe Info  		100.00  LDD0243  [3]
STPyne
 Probe Info  		K408(7.18)  LDD0277  [4]
DA-P3
 Probe Info  		4.82  LDD0182  [5]
AHL-Pu-1
 Probe Info  		C482(3.68)  LDD0170  [6]
HPAP
 Probe Info  		4.01  LDD0063  [7]
DBIA
 Probe Info  		C482(1.08)  LDD0078  [8]
5E-2FA
 Probe Info  		N.A.  LDD2235  [9]
ATP probe
 Probe Info  		K428(0.00); K440(0.00); K562(0.00)  LDD0199  [10]
4-Iodoacetamidophenylacetylene
 Probe Info  		C482(0.00); C433(0.00)  LDD0038  [11]
IA-alkyne
 Probe Info  		C482(0.00); C433(0.00)  LDD0036  [11]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [12]
Lodoacetamide azide
 Probe Info  		C482(0.00); C433(0.00)  LDD0037  [11]
WYneO
 Probe Info  		N.A.  LDD0022  [13]
Compound 10
 Probe Info  		N.A.  LDD2216  [14]
Compound 11
 Probe Info  		N.A.  LDD2213  [14]
ENE
 Probe Info  		N.A.  LDD0006  [13]
IPM
 Probe Info  		N.A.  LDD0005  [13]
VSF
 Probe Info  		N.A.  LDD0007  [13]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [15]
Ox-W18
 Probe Info  		N.A.  LDD2175  [16]
1c-yne
 Probe Info  		N.A.  LDD0228  [17]
Acrolein
 Probe Info  		N.A.  LDD0217  [18]
AOyne
 Probe Info  		13.60  LDD0443  [19]
NAIA_5
 Probe Info  		C482(0.00); C433(0.00)  LDD2223  [20]
HHS-465
 Probe Info  		Y446(0.00); Y429(0.00)  LDD2240  [21]
PAL-AfBPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		11.55  LDD0471  [22]
FFF probe13
 Probe Info  		20.00  LDD0475  [22]
FFF probe14
 Probe Info  		11.22  LDD0477  [22]
FFF probe2
 Probe Info  		16.67  LDD0463  [22]
FFF probe3
 Probe Info  		9.54  LDD0465  [22]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C482(3.68)  LDD0170  [6]
 LDCM0026  4SU-RNA+native RNA DM93 C482(3.46)  LDD0171  [6]
 LDCM0214  AC1 HCT 116 C433(0.93); C482(1.18)  LDD0531  [8]
 LDCM0215  AC10 HCT 116 C433(0.90); C482(1.22)  LDD0532  [8]
 LDCM0216  AC100 HCT 116 C482(0.95)  LDD0533  [8]
 LDCM0217  AC101 HCT 116 C482(0.99)  LDD0534  [8]
 LDCM0218  AC102 HCT 116 C482(0.95)  LDD0535  [8]
 LDCM0219  AC103 HCT 116 C482(1.23)  LDD0536  [8]
 LDCM0220  AC104 HCT 116 C482(1.20)  LDD0537  [8]
 LDCM0221  AC105 HCT 116 C482(1.16)  LDD0538  [8]
 LDCM0222  AC106 HCT 116 C482(1.22)  LDD0539  [8]
 LDCM0223  AC107 HCT 116 C482(1.16)  LDD0540  [8]
 LDCM0224  AC108 HCT 116 C482(0.83)  LDD0541  [8]
 LDCM0225  AC109 HCT 116 C482(0.86)  LDD0542  [8]
 LDCM0226  AC11 HCT 116 C433(1.18); C482(1.47)  LDD0543  [8]
 LDCM0227  AC110 HCT 116 C482(0.86)  LDD0544  [8]
 LDCM0228  AC111 HCT 116 C482(0.87)  LDD0545  [8]
 LDCM0229  AC112 HCT 116 C482(1.02)  LDD0546  [8]
 LDCM0230  AC113 HCT 116 C433(0.99); C482(0.90)  LDD0547  [8]
 LDCM0231  AC114 HCT 116 C433(0.99); C482(1.31)  LDD0548  [8]
 LDCM0232  AC115 HCT 116 C433(0.91); C482(1.36)  LDD0549  [8]
 LDCM0233  AC116 HCT 116 C433(1.15); C482(1.35)  LDD0550  [8]
 LDCM0234  AC117 HCT 116 C433(1.10); C482(1.13)  LDD0551  [8]
 LDCM0235  AC118 HCT 116 C433(1.06); C482(1.06)  LDD0552  [8]
 LDCM0236  AC119 HCT 116 C433(0.90); C482(1.25)  LDD0553  [8]
 LDCM0237  AC12 HCT 116 C433(1.11); C482(1.15)  LDD0554  [8]
 LDCM0238  AC120 HCT 116 C433(1.00); C482(1.25)  LDD0555  [8]
 LDCM0239  AC121 HCT 116 C433(1.26); C482(1.10)  LDD0556  [8]
 LDCM0240  AC122 HCT 116 C433(1.06); C482(1.30)  LDD0557  [8]
 LDCM0241  AC123 HCT 116 C433(1.19); C482(1.05)  LDD0558  [8]
 LDCM0242  AC124 HCT 116 C433(1.24); C482(1.03)  LDD0559  [8]
 LDCM0243  AC125 HCT 116 C433(1.29); C482(1.18)  LDD0560  [8]
 LDCM0244  AC126 HCT 116 C433(1.07); C482(1.41)  LDD0561  [8]
 LDCM0245  AC127 HCT 116 C433(1.03); C482(1.33)  LDD0562  [8]
 LDCM0246  AC128 HCT 116 C433(0.90); C482(1.23)  LDD0563  [8]
 LDCM0247  AC129 HCT 116 C433(0.98); C482(0.89)  LDD0564  [8]
 LDCM0249  AC130 HCT 116 C433(0.87); C482(1.12)  LDD0566  [8]
 LDCM0250  AC131 HCT 116 C433(0.88); C482(0.89)  LDD0567  [8]
 LDCM0251  AC132 HCT 116 C433(0.91); C482(1.03)  LDD0568  [8]
 LDCM0252  AC133 HCT 116 C433(0.92); C482(1.10)  LDD0569  [8]
 LDCM0253  AC134 HCT 116 C433(0.87); C482(1.24)  LDD0570  [8]
 LDCM0254  AC135 HCT 116 C433(0.97); C482(1.16)  LDD0571  [8]
 LDCM0255  AC136 HCT 116 C433(1.08); C482(1.16)  LDD0572  [8]
 LDCM0256  AC137 HCT 116 C433(0.96); C482(1.14)  LDD0573  [8]
 LDCM0257  AC138 HCT 116 C433(1.08); C482(1.27)  LDD0574  [8]
 LDCM0258  AC139 HCT 116 C433(0.82); C482(1.24)  LDD0575  [8]
 LDCM0259  AC14 HCT 116 C433(1.00); C482(1.19)  LDD0576  [8]
 LDCM0260  AC140 HCT 116 C433(0.87); C482(1.38)  LDD0577  [8]
 LDCM0261  AC141 HCT 116 C433(0.90); C482(1.25)  LDD0578  [8]
 LDCM0262  AC142 HCT 116 C433(0.95); C482(1.01)  LDD0579  [8]
 LDCM0263  AC143 HCT 116 C433(0.96); C482(1.15)  LDD0580  [8]
 LDCM0264  AC144 HCT 116 C433(0.90); C482(1.46)  LDD0581  [8]
 LDCM0265  AC145 HCT 116 C433(0.87); C482(1.50)  LDD0582  [8]
 LDCM0266  AC146 HCT 116 C433(0.96); C482(1.64)  LDD0583  [8]
 LDCM0267  AC147 HCT 116 C433(0.88); C482(1.49)  LDD0584  [8]
 LDCM0268  AC148 HCT 116 C433(0.84); C482(1.97)  LDD0585  [8]
 LDCM0269  AC149 HCT 116 C433(0.89); C482(1.69)  LDD0586  [8]
 LDCM0270  AC15 HCT 116 C482(1.13); C433(1.20)  LDD0587  [8]
 LDCM0271  AC150 HCT 116 C433(0.95); C482(1.09)  LDD0588  [8]
 LDCM0272  AC151 HCT 116 C433(0.99); C482(1.11)  LDD0589  [8]
 LDCM0273  AC152 HCT 116 C433(0.93); C482(1.45)  LDD0590  [8]
 LDCM0274  AC153 HCT 116 C433(1.01); C482(2.26)  LDD0591  [8]
 LDCM0621  AC154 HCT 116 C433(0.95); C482(1.33)  LDD2158  [8]
 LDCM0622  AC155 HCT 116 C433(0.91); C482(1.45)  LDD2159  [8]
 LDCM0623  AC156 HCT 116 C433(1.09); C482(1.11)  LDD2160  [8]
 LDCM0624  AC157 HCT 116 C433(1.06); C482(0.93)  LDD2161  [8]
 LDCM0276  AC17 HCT 116 C433(0.90); C482(1.05)  LDD0593  [8]
 LDCM0277  AC18 HCT 116 C433(1.01); C482(1.35)  LDD0594  [8]
 LDCM0278  AC19 HCT 116 C433(0.91); C482(1.09)  LDD0595  [8]
 LDCM0279  AC2 HCT 116 C433(0.98); C482(1.10)  LDD0596  [8]
 LDCM0280  AC20 HCT 116 C433(0.98); C482(1.01)  LDD0597  [8]
 LDCM0281  AC21 HCT 116 C433(0.83); C482(1.12)  LDD0598  [8]
 LDCM0282  AC22 HCT 116 C433(0.80); C482(0.98)  LDD0599  [8]
 LDCM0283  AC23 HCT 116 C433(0.80); C482(1.05)  LDD0600  [8]
 LDCM0284  AC24 HCT 116 C433(0.85); C482(0.90)  LDD0601  [8]
 LDCM0285  AC25 HCT 116 C482(0.90)  LDD0602  [8]
 LDCM0286  AC26 HCT 116 C482(0.93)  LDD0603  [8]
 LDCM0287  AC27 HCT 116 C482(1.16)  LDD0604  [8]
 LDCM0288  AC28 HCT 116 C482(1.15)  LDD0605  [8]
 LDCM0289  AC29 HCT 116 C482(1.34)  LDD0606  [8]
 LDCM0290  AC3 HCT 116 C433(1.02); C482(1.12)  LDD0607  [8]
 LDCM0291  AC30 HCT 116 C482(1.16)  LDD0608  [8]
 LDCM0292  AC31 HCT 116 C482(1.09)  LDD0609  [8]
 LDCM0293  AC32 HCT 116 C482(1.25)  LDD0610  [8]
 LDCM0294  AC33 HCT 116 C482(1.15)  LDD0611  [8]
 LDCM0295  AC34 HCT 116 C482(1.36)  LDD0612  [8]
 LDCM0296  AC35 HCT 116 C433(0.83); C482(0.91)  LDD0613  [8]
 LDCM0297  AC36 HCT 116 C433(0.81); C482(1.00)  LDD0614  [8]
 LDCM0298  AC37 HCT 116 C482(1.04); C433(1.26)  LDD0615  [8]
 LDCM0299  AC38 HCT 116 C482(0.93); C433(0.95)  LDD0616  [8]
 LDCM0300  AC39 HCT 116 C433(0.93); C482(1.03)  LDD0617  [8]
 LDCM0301  AC4 HCT 116 C433(1.03); C482(1.27)  LDD0618  [8]
 LDCM0302  AC40 HCT 116 C433(0.97); C482(1.25)  LDD0619  [8]
 LDCM0303  AC41 HCT 116 C433(0.79); C482(1.13)  LDD0620  [8]
 LDCM0304  AC42 HCT 116 C433(0.76); C482(1.01)  LDD0621  [8]
 LDCM0305  AC43 HCT 116 C433(0.62); C482(1.17)  LDD0622  [8]
 LDCM0306  AC44 HCT 116 C482(1.00); C433(1.03)  LDD0623  [8]
 LDCM0307  AC45 HCT 116 C433(0.94); C482(1.23)  LDD0624  [8]
 LDCM0308  AC46 HCT 116 C482(1.17)  LDD0625  [8]
 LDCM0309  AC47 HCT 116 C482(1.17)  LDD0626  [8]
 LDCM0310  AC48 HCT 116 C482(1.28)  LDD0627  [8]
 LDCM0311  AC49 HCT 116 C482(1.50)  LDD0628  [8]
 LDCM0312  AC5 HCT 116 C433(1.12); C482(1.34)  LDD0629  [8]
 LDCM0313  AC50 HCT 116 C482(1.37)  LDD0630  [8]
 LDCM0314  AC51 HCT 116 C482(0.94)  LDD0631  [8]
 LDCM0315  AC52 HCT 116 C482(1.28)  LDD0632  [8]
 LDCM0316  AC53 HCT 116 C482(1.16)  LDD0633  [8]
 LDCM0317  AC54 HCT 116 C482(1.14)  LDD0634  [8]
 LDCM0318  AC55 HCT 116 C482(1.32)  LDD0635  [8]
 LDCM0319  AC56 HCT 116 C482(1.74)  LDD0636  [8]
 LDCM0320  AC57 HCT 116 C433(0.76); C482(1.59)  LDD0637  [8]
 LDCM0321  AC58 HCT 116 C433(0.73); C482(1.61)  LDD0638  [8]
 LDCM0322  AC59 HCT 116 C433(0.76); C482(1.53)  LDD0639  [8]
 LDCM0323  AC6 HCT 116 C433(1.13); C482(1.14)  LDD0640  [8]
 LDCM0324  AC60 HCT 116 C433(0.71); C482(1.63)  LDD0641  [8]
 LDCM0325  AC61 HCT 116 C433(0.80); C482(1.59)  LDD0642  [8]
 LDCM0326  AC62 HCT 116 C433(0.68); C482(2.04)  LDD0643  [8]
 LDCM0327  AC63 HCT 116 C433(0.59); C482(1.69)  LDD0644  [8]
 LDCM0328  AC64 HCT 116 C433(0.61); C482(1.93)  LDD0645  [8]
 LDCM0329  AC65 HCT 116 C433(0.65); C482(1.80)  LDD0646  [8]
 LDCM0330  AC66 HCT 116 C433(0.62); C482(1.84)  LDD0647  [8]
 LDCM0331  AC67 HCT 116 C433(0.73); C482(2.20)  LDD0648  [8]
 LDCM0332  AC68 HCT 116 C433(1.18); C482(1.27)  LDD0649  [8]
 LDCM0333  AC69 HCT 116 C433(0.60); C482(1.26)  LDD0650  [8]
 LDCM0334  AC7 HCT 116 C433(0.98); C482(1.06)  LDD0651  [8]
 LDCM0335  AC70 HCT 116 C433(0.77); C482(1.55)  LDD0652  [8]
 LDCM0336  AC71 HCT 116 C433(0.89); C482(1.16)  LDD0653  [8]
 LDCM0337  AC72 HCT 116 C433(0.61); C482(1.27)  LDD0654  [8]
 LDCM0338  AC73 HCT 116 C433(0.72); C482(1.66)  LDD0655  [8]
 LDCM0339  AC74 HCT 116 C433(0.91); C482(1.61)  LDD0656  [8]
 LDCM0340  AC75 HCT 116 C433(0.76); C482(1.88)  LDD0657  [8]
 LDCM0341  AC76 HCT 116 C433(0.80); C482(1.39)  LDD0658  [8]
 LDCM0342  AC77 HCT 116 C433(0.76); C482(1.43)  LDD0659  [8]
 LDCM0343  AC78 HCT 116 C482(1.11); C433(1.16)  LDD0660  [8]
 LDCM0344  AC79 HCT 116 C433(1.15); C482(1.36)  LDD0661  [8]
 LDCM0345  AC8 HCT 116 C433(1.09); C482(1.34)  LDD0662  [8]
 LDCM0346  AC80 HCT 116 C433(0.65); C482(1.20)  LDD0663  [8]
 LDCM0347  AC81 HCT 116 C433(1.04); C482(1.16)  LDD0664  [8]
 LDCM0348  AC82 HCT 116 C433(0.66); C482(2.01)  LDD0665  [8]
 LDCM0349  AC83 HCT 116 C433(0.79); C482(1.75)  LDD0666  [8]
 LDCM0350  AC84 HCT 116 C433(0.77); C482(1.68)  LDD0667  [8]
 LDCM0351  AC85 HCT 116 C433(0.75); C482(1.43)  LDD0668  [8]
 LDCM0352  AC86 HCT 116 C433(0.68); C482(1.20)  LDD0669  [8]
 LDCM0353  AC87 HCT 116 C433(0.77); C482(1.12)  LDD0670  [8]
 LDCM0354  AC88 HCT 116 C433(0.71); C482(1.13)  LDD0671  [8]
 LDCM0355  AC89 HCT 116 C433(0.72); C482(1.34)  LDD0672  [8]
 LDCM0357  AC90 HCT 116 C433(0.91); C482(1.08)  LDD0674  [8]
 LDCM0358  AC91 HCT 116 C433(0.71); C482(1.73)  LDD0675  [8]
 LDCM0359  AC92 HCT 116 C433(0.78); C482(1.66)  LDD0676  [8]
 LDCM0360  AC93 HCT 116 C433(0.76); C482(1.35)  LDD0677  [8]
 LDCM0361  AC94 HCT 116 C433(0.71); C482(1.16)  LDD0678  [8]
 LDCM0362  AC95 HCT 116 C433(0.74); C482(1.13)  LDD0679  [8]
 LDCM0363  AC96 HCT 116 C433(0.63); C482(1.23)  LDD0680  [8]
 LDCM0364  AC97 HCT 116 C433(0.70); C482(1.53)  LDD0681  [8]
 LDCM0365  AC98 HCT 116 C482(1.64)  LDD0682  [8]
 LDCM0366  AC99 HCT 116 C482(0.91)  LDD0683  [8]
 LDCM0248  AKOS034007472 HCT 116 C433(0.92); C482(1.01)  LDD0565  [8]
 LDCM0356  AKOS034007680 HCT 116 C433(1.04); C482(1.17)  LDD0673  [8]
 LDCM0275  AKOS034007705 HCT 116 C433(1.17); C482(1.62)  LDD0592  [8]
 LDCM0156  Aniline NCI-H1299 12.28  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C482(1.08)  LDD0078  [8]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [18]
 LDCM0632  CL-Sc Hep-G2 C482(0.92)  LDD2227  [20]
 LDCM0367  CL1 HCT 116 C482(0.96); C433(1.03)  LDD0684  [8]
 LDCM0368  CL10 HCT 116 C433(1.00); C482(1.29)  LDD0685  [8]
 LDCM0369  CL100 HCT 116 C433(0.96); C482(1.20)  LDD0686  [8]
 LDCM0370  CL101 HCT 116 C433(0.90); C482(1.15)  LDD0687  [8]
 LDCM0371  CL102 HCT 116 C482(0.95); C433(1.09)  LDD0688  [8]
 LDCM0372  CL103 HCT 116 C433(0.98); C482(0.99)  LDD0689  [8]
 LDCM0373  CL104 HCT 116 C482(1.05); C433(1.17)  LDD0690  [8]
 LDCM0374  CL105 HCT 116 C433(1.08); C482(1.21)  LDD0691  [8]
 LDCM0375  CL106 HCT 116 C433(1.17); C482(1.59)  LDD0692  [8]
 LDCM0376  CL107 HCT 116 C433(1.06); C482(1.52)  LDD0693  [8]
 LDCM0377  CL108 HCT 116 C433(0.98); C482(1.48)  LDD0694  [8]
 LDCM0378  CL109 HCT 116 C433(0.85); C482(1.23)  LDD0695  [8]
 LDCM0379  CL11 HCT 116 C433(1.08); C482(1.52)  LDD0696  [8]
 LDCM0380  CL110 HCT 116 C433(0.81); C482(1.31)  LDD0697  [8]
 LDCM0381  CL111 HCT 116 C433(0.87); C482(1.19)  LDD0698  [8]
 LDCM0382  CL112 HCT 116 C482(0.95)  LDD0699  [8]
 LDCM0383  CL113 HCT 116 C482(1.20)  LDD0700  [8]
 LDCM0384  CL114 HCT 116 C482(1.11)  LDD0701  [8]
 LDCM0385  CL115 HCT 116 C482(1.23)  LDD0702  [8]
 LDCM0386  CL116 HCT 116 C482(0.99)  LDD0703  [8]
 LDCM0387  CL117 HCT 116 C433(0.83); C482(1.65)  LDD0704  [8]
 LDCM0388  CL118 HCT 116 C433(0.65); C482(1.04)  LDD0705  [8]
 LDCM0389  CL119 HCT 116 C433(0.66); C482(1.28)  LDD0706  [8]
 LDCM0390  CL12 HCT 116 C433(0.97); C482(1.53)  LDD0707  [8]
 LDCM0391  CL120 HCT 116 C433(0.64); C482(1.10)  LDD0708  [8]
 LDCM0392  CL121 HCT 116 C482(0.93)  LDD0709  [8]
 LDCM0393  CL122 HCT 116 C482(1.02)  LDD0710  [8]
 LDCM0394  CL123 HCT 116 C482(1.34)  LDD0711  [8]
 LDCM0395  CL124 HCT 116 C482(1.34)  LDD0712  [8]
 LDCM0396  CL125 HCT 116 C433(0.82); C482(1.28)  LDD0713  [8]
 LDCM0397  CL126 HCT 116 C433(0.94); C482(1.41)  LDD0714  [8]
 LDCM0398  CL127 HCT 116 C433(0.90); C482(1.29)  LDD0715  [8]
 LDCM0399  CL128 HCT 116 C433(0.95); C482(1.44)  LDD0716  [8]
 LDCM0400  CL13 HCT 116 C482(1.34); C433(1.37)  LDD0717  [8]
 LDCM0401  CL14 HCT 116 C433(1.02); C482(1.03)  LDD0718  [8]
 LDCM0402  CL15 HCT 116 C433(1.04); C482(1.12)  LDD0719  [8]
 LDCM0403  CL16 HCT 116 C433(0.95); C482(1.14)  LDD0720  [8]
 LDCM0404  CL17 HCT 116 C433(0.93); C482(1.02)  LDD0721  [8]
 LDCM0405  CL18 HCT 116 C433(0.75); C482(1.14)  LDD0722  [8]
 LDCM0406  CL19 HCT 116 C433(0.73); C482(1.19)  LDD0723  [8]
 LDCM0407  CL2 HCT 116 C482(0.91); C433(0.94)  LDD0724  [8]
 LDCM0408  CL20 HCT 116 C433(0.85); C482(1.35)  LDD0725  [8]
 LDCM0409  CL21 HCT 116 C433(0.78); C482(1.46)  LDD0726  [8]
 LDCM0410  CL22 HCT 116 C433(0.76); C482(1.99)  LDD0727  [8]
 LDCM0411  CL23 HCT 116 C433(0.85); C482(1.12)  LDD0728  [8]
 LDCM0412  CL24 HCT 116 C433(0.89); C482(1.35)  LDD0729  [8]
 LDCM0413  CL25 HCT 116 C433(0.84); C482(1.36)  LDD0730  [8]
 LDCM0414  CL26 HCT 116 C433(0.94); C482(1.10)  LDD0731  [8]
 LDCM0415  CL27 HCT 116 C433(0.87); C482(0.99)  LDD0732  [8]
 LDCM0416  CL28 HCT 116 C433(0.80); C482(1.18)  LDD0733  [8]
 LDCM0417  CL29 HCT 116 C433(0.79); C482(1.32)  LDD0734  [8]
 LDCM0418  CL3 HCT 116 C433(0.87); C482(0.92)  LDD0735  [8]
 LDCM0419  CL30 HCT 116 C433(0.88); C482(1.04)  LDD0736  [8]
 LDCM0420  CL31 HCT 116 C433(0.80); C482(1.29)  LDD0737  [8]
 LDCM0421  CL32 HCT 116 C433(0.74); C482(1.41)  LDD0738  [8]
 LDCM0422  CL33 HCT 116 C433(0.64); C482(1.26)  LDD0739  [8]
 LDCM0423  CL34 HCT 116 C433(0.68); C482(1.51)  LDD0740  [8]
 LDCM0424  CL35 HCT 116 C433(0.79); C482(1.36)  LDD0741  [8]
 LDCM0425  CL36 HCT 116 C433(0.74); C482(1.59)  LDD0742  [8]
 LDCM0426  CL37 HCT 116 C433(0.76); C482(1.62)  LDD0743  [8]
 LDCM0428  CL39 HCT 116 C433(0.89); C482(1.36)  LDD0745  [8]
 LDCM0429  CL4 HCT 116 C433(1.23); C482(0.95)  LDD0746  [8]
 LDCM0430  CL40 HCT 116 C433(0.76); C482(1.25)  LDD0747  [8]
 LDCM0431  CL41 HCT 116 C433(0.75); C482(1.35)  LDD0748  [8]
 LDCM0432  CL42 HCT 116 C433(0.81); C482(1.78)  LDD0749  [8]
 LDCM0433  CL43 HCT 116 C433(0.76); C482(1.71)  LDD0750  [8]
 LDCM0434  CL44 HCT 116 C433(0.67); C482(1.54)  LDD0751  [8]
 LDCM0435  CL45 HCT 116 C433(0.60); C482(1.77)  LDD0752  [8]
 LDCM0436  CL46 HCT 116 C433(0.92); C482(0.77)  LDD0753  [8]
 LDCM0437  CL47 HCT 116 C433(0.91); C482(0.99)  LDD0754  [8]
 LDCM0438  CL48 HCT 116 C433(0.94); C482(0.79)  LDD0755  [8]
 LDCM0439  CL49 HCT 116 C433(0.87); C482(0.84)  LDD0756  [8]
 LDCM0440  CL5 HCT 116 C433(0.93); C482(0.99)  LDD0757  [8]
 LDCM0441  CL50 HCT 116 C433(0.87); C482(0.78)  LDD0758  [8]
 LDCM0442  CL51 HCT 116 C433(0.99); C482(0.84)  LDD0759  [8]
 LDCM0443  CL52 HCT 116 C433(1.03); C482(0.87)  LDD0760  [8]
 LDCM0444  CL53 HCT 116 C433(1.22); C482(0.80)  LDD0761  [8]
 LDCM0445  CL54 HCT 116 C433(0.81); C482(0.84)  LDD0762  [8]
 LDCM0446  CL55 HCT 116 C433(0.86); C482(0.73)  LDD0763  [8]
 LDCM0447  CL56 HCT 116 C433(0.96); C482(0.87)  LDD0764  [8]
 LDCM0448  CL57 HCT 116 C433(1.10); C482(0.80)  LDD0765  [8]
 LDCM0449  CL58 HCT 116 C433(1.01); C482(0.75)  LDD0766  [8]
 LDCM0450  CL59 HCT 116 C433(0.99); C482(0.82)  LDD0767  [8]
 LDCM0451  CL6 HCT 116 C433(0.83); C482(1.17)  LDD0768  [8]
 LDCM0452  CL60 HCT 116 C433(0.94); C482(0.79)  LDD0769  [8]
 LDCM0453  CL61 HCT 116 C433(0.99); C482(1.17)  LDD0770  [8]
 LDCM0454  CL62 HCT 116 C433(1.16); C482(1.20)  LDD0771  [8]
 LDCM0455  CL63 HCT 116 C433(0.99); C482(1.35)  LDD0772  [8]
 LDCM0456  CL64 HCT 116 C433(0.84); C482(1.25)  LDD0773  [8]
 LDCM0457  CL65 HCT 116 C433(1.13); C482(1.20)  LDD0774  [8]
 LDCM0458  CL66 HCT 116 C433(1.25); C482(1.55)  LDD0775  [8]
 LDCM0459  CL67 HCT 116 C433(1.09); C482(1.27)  LDD0776  [8]
 LDCM0460  CL68 HCT 116 C433(1.03); C482(1.25)  LDD0777  [8]
 LDCM0461  CL69 HCT 116 C433(0.90); C482(1.16)  LDD0778  [8]
 LDCM0462  CL7 HCT 116 C433(1.14); C482(1.37)  LDD0779  [8]
 LDCM0463  CL70 HCT 116 C433(0.95); C482(1.30)  LDD0780  [8]
 LDCM0464  CL71 HCT 116 C433(1.19); C482(1.37)  LDD0781  [8]
 LDCM0465  CL72 HCT 116 C433(0.94); C482(1.06)  LDD0782  [8]
 LDCM0466  CL73 HCT 116 C433(0.93); C482(1.25)  LDD0783  [8]
 LDCM0467  CL74 HCT 116 C433(0.90); C482(1.18)  LDD0784  [8]
 LDCM0469  CL76 HCT 116 C482(1.10)  LDD0786  [8]
 LDCM0470  CL77 HCT 116 C482(1.04)  LDD0787  [8]
 LDCM0471  CL78 HCT 116 C482(1.11)  LDD0788  [8]
 LDCM0472  CL79 HCT 116 C482(1.25)  LDD0789  [8]
 LDCM0473  CL8 HCT 116 C433(0.68); C482(1.57)  LDD0790  [8]
 LDCM0474  CL80 HCT 116 C482(0.98)  LDD0791  [8]
 LDCM0475  CL81 HCT 116 C482(1.08)  LDD0792  [8]
 LDCM0476  CL82 HCT 116 C482(1.47)  LDD0793  [8]
 LDCM0477  CL83 HCT 116 C482(1.32)  LDD0794  [8]
 LDCM0478  CL84 HCT 116 C482(1.61)  LDD0795  [8]
 LDCM0479  CL85 HCT 116 C482(1.12)  LDD0796  [8]
 LDCM0480  CL86 HCT 116 C482(1.09)  LDD0797  [8]
 LDCM0481  CL87 HCT 116 C482(1.18)  LDD0798  [8]
 LDCM0482  CL88 HCT 116 C482(1.35)  LDD0799  [8]
 LDCM0483  CL89 HCT 116 C482(1.78)  LDD0800  [8]
 LDCM0484  CL9 HCT 116 C433(1.07); C482(1.26)  LDD0801  [8]
 LDCM0485  CL90 HCT 116 C482(0.94)  LDD0802  [8]
 LDCM0486  CL91 HCT 116 C433(0.96); C482(1.10)  LDD0803  [8]
 LDCM0487  CL92 HCT 116 C433(1.02); C482(1.14)  LDD0804  [8]
 LDCM0488  CL93 HCT 116 C433(0.98); C482(1.04)  LDD0805  [8]
 LDCM0489  CL94 HCT 116 C433(1.02); C482(1.16)  LDD0806  [8]
 LDCM0490  CL95 HCT 116 C433(0.97); C482(1.16)  LDD0807  [8]
 LDCM0491  CL96 HCT 116 C433(1.01); C482(1.06)  LDD0808  [8]
 LDCM0492  CL97 HCT 116 C433(0.92); C482(1.10)  LDD0809  [8]
 LDCM0493  CL98 HCT 116 C433(0.97); C482(1.31)  LDD0810  [8]
 LDCM0494  CL99 HCT 116 C433(0.94); C482(1.31)  LDD0811  [8]
 LDCM0634  CY-0357 Hep-G2 C482(0.33)  LDD2228  [20]
 LDCM0495  E2913 HEK-293T C482(0.87); C433(1.17)  LDD1698  [23]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C482(1.22)  LDD1702  [24]
 LDCM0625  F8 Ramos C482(1.57)  LDD2187  [25]
 LDCM0572  Fragment10 Ramos C482(5.39)  LDD2189  [25]
 LDCM0573  Fragment11 Ramos C482(0.17)  LDD2190  [25]
 LDCM0574  Fragment12 Ramos C482(0.87)  LDD2191  [25]
 LDCM0575  Fragment13 Ramos C482(1.12)  LDD2192  [25]
 LDCM0576  Fragment14 Ramos C482(0.78)  LDD2193  [25]
 LDCM0579  Fragment20 Ramos C482(1.05)  LDD2194  [25]
 LDCM0580  Fragment21 Ramos C482(1.03)  LDD2195  [25]
 LDCM0582  Fragment23 Ramos C482(0.99)  LDD2196  [25]
 LDCM0578  Fragment27 Ramos C482(1.34)  LDD2197  [25]
 LDCM0586  Fragment28 Ramos C482(0.95)  LDD2198  [25]
 LDCM0588  Fragment30 Ramos C482(1.22)  LDD2199  [25]
 LDCM0589  Fragment31 Ramos C482(1.06)  LDD2200  [25]
 LDCM0590  Fragment32 Ramos C482(1.55)  LDD2201  [25]
 LDCM0468  Fragment33 HCT 116 C433(0.98); C482(1.23)  LDD0785  [8]
 LDCM0596  Fragment38 Ramos C482(1.04)  LDD2203  [25]
 LDCM0566  Fragment4 Ramos C482(0.85)  LDD2184  [25]
 LDCM0427  Fragment51 HCT 116 C433(0.77); C482(1.88)  LDD0744  [8]
 LDCM0610  Fragment52 Ramos C482(0.89)  LDD2204  [25]
 LDCM0614  Fragment56 Ramos C482(0.95)  LDD2205  [25]
 LDCM0569  Fragment7 Ramos C482(1.36)  LDD2186  [25]
 LDCM0571  Fragment9 Ramos C482(1.05)  LDD2188  [25]
 LDCM0107  IAA HeLa N.A.  LDD0221  [18]
 LDCM0022  KB02 HCT 116 C482(1.79); C433(1.14)  LDD0080  [8]
 LDCM0023  KB03 HCT 116 C482(1.01); C433(1.29)  LDD0081  [8]
 LDCM0024  KB05 HCT 116 C482(1.44); C433(1.24)  LDD0082  [8]
 LDCM0030  Luteolin HEK-293T 4.82  LDD0182  [5]
 LDCM0109  NEM HeLa H559(0.00); H549(0.00)  LDD0223  [18]
 LDCM0627  NUDT7-COV-1 HEK-293T C482(0.87)  LDD2206  [26]
 LDCM0628  OTUB2-COV-1 HEK-293T C482(0.20)  LDD2207  [26]
 LDCM0014  Panhematin K562 4.01  LDD0063  [7]
 LDCM0131  RA190 MM1.R C482(0.92)  LDD0304  [27]
 LDCM0021  THZ1 HCT 116 C482(1.07); C433(1.28)  LDD2173  [8]

References

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Mass spectrometry data entry: PXD037665
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
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Mass spectrometry data entry: PXD028270
5 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
8 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
9 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
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Mass spectrometry data entry: PXD028853
12 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
13 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
14 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
15 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
16 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
17 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
18 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
19 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
20 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
21 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
22 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
23 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
24 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
25 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
26 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
27 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.