Details of the Target

General Information of Target

Target ID LDTP10822
Target Name Protein IWS1 homolog (IWS1)
Gene Name IWS1
Gene ID 55677
Synonyms
IWS1L; Protein IWS1 homolog; IWS1-like protein
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MEATGTWALLLALALLLLLTLALSGTRARGHLPPGPTPLPLLGNLLQLRPGALYSGLMRL
SKKYGPVFTIYLGPWRPVVVLVGQEAVREALGGQAEEFSGRGTVAMLEGTFDGHGVFFSN
GERWRQLRKFTMLALRDLGMGKREGEELIQAEARCLVETFQGTEGRPFDPSLLLAQATSN
VVCSLLFGLRFSYEDKEFQAVVRAAGGTLLGVSSQGGQTYEMFSWFLRPLPGPHKQLLHH
VSTLAAFTVRQVQQHQGNLDASGPARDLVDAFLLKMAQEEQNPGTEFTNKNMLMTVIYLL
FAGTMTVSTTVGYTLLLLMKYPHVQKWVREELNRELGAGQAPSLGDRTRLPYTDAVLHEA
QRLLALVPMGIPRTLMRTTRFRGYTLPQGTEVFPLLGSILHDPNIFKHPEEFNPDRFLDA
DGRFRKHEAFLPFSLGKRVCLGEGLAKAELFLFFTTILQAFSLESPCPPDTLSLKPTVSG
LFNIPPAFQLQVRPTDLHSTTQTR
Target Bioclass
Other
Family
IWS1 family
Subcellular location
Nucleus
Function
Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription.
Uniprot ID
Q96ST2
Ensemble ID
ENST00000295321.9
HGNC ID
HGNC:25467

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
769P SNV: p.K783N .
CAL51 Deletion: p.S802AfsTer7 .
CMK SNV: p.S159F .
FTC133 SNV: p.P755T .
HEC1B SNV: p.E227D .
KYSE510 Substitution: p.T693I .
MCC13 SNV: p.P204L .
NCIH146 SNV: p.G744V .
NUGC3 Insertion: p.S802KfsTer12 .
U2OS SNV: p.E486Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 27 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TH211
 Probe Info  		Y762(10.46)  LDD0260  [2]
STPyne
 Probe Info  		K653(10.00)  LDD0277  [3]
Probe 1
 Probe Info  		Y762(37.01)  LDD3495  [4]
Alkylaryl probe 3
 Probe Info  		20.00  LDD0382  [5]
BTD
 Probe Info  		C749(1.38)  LDD1700  [6]
AHL-Pu-1
 Probe Info  		C749(2.50)  LDD0169  [7]
HHS-475
 Probe Info  		Y665(0.99)  LDD0264  [8]
HHS-465
 Probe Info  		Y665(10.00)  LDD2237  [9]
DBIA
 Probe Info  		C749(0.82)  LDD0531  [10]
W1
 Probe Info  		C749(0.56)  LDD0239  [11]
ATP probe
 Probe Info  		N.A.  LDD0199  [12]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [13]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [13]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [13]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [14]
NAIA_5
 Probe Info  		N.A.  LDD2224  [15]
TFBX
 Probe Info  		N.A.  LDD0027  [14]
WYneN
 Probe Info  		N.A.  LDD0021  [16]
Compound 10
 Probe Info  		N.A.  LDD2216  [17]
IPM
 Probe Info  		N.A.  LDD0005  [16]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [18]
1c-yne
 Probe Info  		N.A.  LDD0228  [19]
Acrolein
 Probe Info  		N.A.  LDD0217  [20]
Methacrolein
 Probe Info  		N.A.  LDD0218  [20]
AOyne
 Probe Info  		9.20  LDD0443  [21]
HHS-482
 Probe Info  		Y665(0.93)  LDD2239  [9]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C391
 Probe Info  		13.83  LDD2050  [22]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C749(0.75)  LDD2142  [6]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C749(0.83)  LDD2112  [6]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C749(0.73)  LDD2117  [6]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C749(1.08)  LDD2152  [6]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C749(1.22)  LDD2103  [6]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C749(0.49)  LDD2132  [6]
 LDCM0026  4SU-RNA+native RNA HEK-293T C749(2.50)  LDD0169  [7]
 LDCM0561  Abegg_cp(-)-10 HeLa C749(2.40)  LDD0312  [14]
 LDCM0214  AC1 HCT 116 C749(0.82)  LDD0531  [10]
 LDCM0215  AC10 HCT 116 C749(0.98)  LDD0532  [10]
 LDCM0226  AC11 HCT 116 C749(1.01)  LDD0543  [10]
 LDCM0237  AC12 HCT 116 C749(0.81)  LDD0554  [10]
 LDCM0259  AC14 HCT 116 C749(0.98)  LDD0576  [10]
 LDCM0270  AC15 HCT 116 C749(0.81)  LDD0587  [10]
 LDCM0276  AC17 HCT 116 C749(0.61)  LDD0593  [10]
 LDCM0277  AC18 HCT 116 C749(0.81)  LDD0594  [10]
 LDCM0278  AC19 HCT 116 C749(0.69)  LDD0595  [10]
 LDCM0279  AC2 HCT 116 C749(0.80)  LDD0596  [10]
 LDCM0280  AC20 HCT 116 C749(0.74)  LDD0597  [10]
 LDCM0281  AC21 HCT 116 C749(0.87)  LDD0598  [10]
 LDCM0282  AC22 HCT 116 C749(0.66)  LDD0599  [10]
 LDCM0283  AC23 HCT 116 C749(0.61)  LDD0600  [10]
 LDCM0284  AC24 HCT 116 C749(0.50)  LDD0601  [10]
 LDCM0285  AC25 HEK-293T C749(1.07)  LDD1524  [23]
 LDCM0286  AC26 HEK-293T C749(1.03)  LDD1525  [23]
 LDCM0287  AC27 HEK-293T C749(0.99)  LDD1526  [23]
 LDCM0288  AC28 HEK-293T C749(0.97)  LDD1527  [23]
 LDCM0289  AC29 HEK-293T C749(1.02)  LDD1528  [23]
 LDCM0290  AC3 HCT 116 C749(0.64)  LDD0607  [10]
 LDCM0291  AC30 HEK-293T C749(1.05)  LDD1530  [23]
 LDCM0292  AC31 HEK-293T C749(1.01)  LDD1531  [23]
 LDCM0293  AC32 HEK-293T C749(1.03)  LDD1532  [23]
 LDCM0294  AC33 HEK-293T C749(1.06)  LDD1533  [23]
 LDCM0295  AC34 HEK-293T C749(1.04)  LDD1534  [23]
 LDCM0296  AC35 HEK-293T C749(0.99)  LDD1535  [23]
 LDCM0297  AC36 HEK-293T C749(1.04)  LDD1536  [23]
 LDCM0298  AC37 HEK-293T C749(1.01)  LDD1537  [23]
 LDCM0299  AC38 HEK-293T C749(0.99)  LDD1538  [23]
 LDCM0300  AC39 HEK-293T C749(0.97)  LDD1539  [23]
 LDCM0301  AC4 HCT 116 C749(0.63)  LDD0618  [10]
 LDCM0302  AC40 HEK-293T C749(0.94)  LDD1541  [23]
 LDCM0303  AC41 HEK-293T C749(1.07)  LDD1542  [23]
 LDCM0304  AC42 HEK-293T C749(1.07)  LDD1543  [23]
 LDCM0305  AC43 HEK-293T C749(0.96)  LDD1544  [23]
 LDCM0306  AC44 HEK-293T C749(1.00)  LDD1545  [23]
 LDCM0307  AC45 HEK-293T C749(1.05)  LDD1546  [23]
 LDCM0308  AC46 HEK-293T C749(1.01)  LDD1547  [23]
 LDCM0309  AC47 HEK-293T C749(1.05)  LDD1548  [23]
 LDCM0310  AC48 HEK-293T C749(0.93)  LDD1549  [23]
 LDCM0311  AC49 HEK-293T C749(1.02)  LDD1550  [23]
 LDCM0312  AC5 HCT 116 C749(0.65)  LDD0629  [10]
 LDCM0313  AC50 HEK-293T C749(1.03)  LDD1552  [23]
 LDCM0314  AC51 HEK-293T C749(0.97)  LDD1553  [23]
 LDCM0315  AC52 HEK-293T C749(0.96)  LDD1554  [23]
 LDCM0316  AC53 HEK-293T C749(1.00)  LDD1555  [23]
 LDCM0317  AC54 HEK-293T C749(0.99)  LDD1556  [23]
 LDCM0318  AC55 HEK-293T C749(1.02)  LDD1557  [23]
 LDCM0319  AC56 HEK-293T C749(0.97)  LDD1558  [23]
 LDCM0320  AC57 HEK-293T C749(1.03)  LDD1559  [23]
 LDCM0321  AC58 HEK-293T C749(1.00)  LDD1560  [23]
 LDCM0322  AC59 HEK-293T C749(0.99)  LDD1561  [23]
 LDCM0323  AC6 HCT 116 C749(0.99)  LDD0640  [10]
 LDCM0324  AC60 HEK-293T C749(0.94)  LDD1563  [23]
 LDCM0325  AC61 HEK-293T C749(1.01)  LDD1564  [23]
 LDCM0326  AC62 HEK-293T C749(1.00)  LDD1565  [23]
 LDCM0327  AC63 HEK-293T C749(1.01)  LDD1566  [23]
 LDCM0328  AC64 HEK-293T C749(0.94)  LDD1567  [23]
 LDCM0334  AC7 HCT 116 C749(0.89)  LDD0651  [10]
 LDCM0345  AC8 HCT 116 C749(0.94)  LDD0662  [10]
 LDCM0520  AKOS000195272 MDA-MB-231 C749(0.65)  LDD2113  [6]
 LDCM0248  AKOS034007472 HCT 116 C749(0.83)  LDD0565  [10]
 LDCM0356  AKOS034007680 HCT 116 C749(0.94)  LDD0673  [10]
 LDCM0275  AKOS034007705 HCT 116 C749(0.85)  LDD0592  [10]
 LDCM0156  Aniline NCI-H1299 11.98  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C749(0.37)  LDD2091  [6]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [20]
 LDCM0632  CL-Sc Hep-G2 C749(20.00)  LDD2227  [15]
 LDCM0367  CL1 HCT 116 C749(1.20)  LDD0684  [10]
 LDCM0368  CL10 HCT 116 C749(1.12)  LDD0685  [10]
 LDCM0369  CL100 HCT 116 C749(0.74)  LDD0686  [10]
 LDCM0370  CL101 HCT 116 C749(0.71)  LDD0687  [10]
 LDCM0371  CL102 HCT 116 C749(0.88)  LDD0688  [10]
 LDCM0372  CL103 HCT 116 C749(1.12)  LDD0689  [10]
 LDCM0373  CL104 HCT 116 C749(0.86)  LDD0690  [10]
 LDCM0374  CL105 HCT 116 C749(0.68)  LDD0691  [10]
 LDCM0375  CL106 HCT 116 C749(0.53)  LDD0692  [10]
 LDCM0376  CL107 HCT 116 C749(0.60)  LDD0693  [10]
 LDCM0377  CL108 HCT 116 C749(0.54)  LDD0694  [10]
 LDCM0378  CL109 HCT 116 C749(0.58)  LDD0695  [10]
 LDCM0379  CL11 HCT 116 C749(1.21)  LDD0696  [10]
 LDCM0380  CL110 HCT 116 C749(0.50)  LDD0697  [10]
 LDCM0381  CL111 HCT 116 C749(0.65)  LDD0698  [10]
 LDCM0382  CL112 HEK-293T C749(1.05)  LDD1586  [23]
 LDCM0383  CL113 HEK-293T C749(0.96)  LDD1587  [23]
 LDCM0384  CL114 HEK-293T C749(1.11)  LDD1588  [23]
 LDCM0385  CL115 HEK-293T C749(1.11)  LDD1589  [23]
 LDCM0386  CL116 HEK-293T C749(1.02)  LDD1590  [23]
 LDCM0387  CL117 HEK-293T C749(0.96)  LDD1591  [23]
 LDCM0388  CL118 HEK-293T C749(0.94)  LDD1592  [23]
 LDCM0389  CL119 HEK-293T C749(1.01)  LDD1593  [23]
 LDCM0390  CL12 HCT 116 C749(1.30)  LDD0707  [10]
 LDCM0391  CL120 HEK-293T C749(0.96)  LDD1595  [23]
 LDCM0392  CL121 HEK-293T C749(1.02)  LDD1596  [23]
 LDCM0393  CL122 HEK-293T C749(1.00)  LDD1597  [23]
 LDCM0394  CL123 HEK-293T C749(1.01)  LDD1598  [23]
 LDCM0395  CL124 HEK-293T C749(0.99)  LDD1599  [23]
 LDCM0396  CL125 HEK-293T C749(0.94)  LDD1600  [23]
 LDCM0397  CL126 HEK-293T C749(0.99)  LDD1601  [23]
 LDCM0398  CL127 HEK-293T C749(1.02)  LDD1602  [23]
 LDCM0399  CL128 HEK-293T C749(0.99)  LDD1603  [23]
 LDCM0400  CL13 HCT 116 C749(1.13)  LDD0717  [10]
 LDCM0401  CL14 HCT 116 C749(1.38)  LDD0718  [10]
 LDCM0402  CL15 HCT 116 C749(1.08)  LDD0719  [10]
 LDCM0403  CL16 HCT 116 C749(1.03)  LDD0720  [10]
 LDCM0404  CL17 HEK-293T C749(1.14)  LDD1608  [23]
 LDCM0405  CL18 HEK-293T C749(1.10)  LDD1609  [23]
 LDCM0406  CL19 HEK-293T C749(0.99)  LDD1610  [23]
 LDCM0407  CL2 HCT 116 C749(1.41)  LDD0724  [10]
 LDCM0408  CL20 HEK-293T C749(0.92)  LDD1612  [23]
 LDCM0409  CL21 HEK-293T C749(0.99)  LDD1613  [23]
 LDCM0410  CL22 HEK-293T C749(1.02)  LDD1614  [23]
 LDCM0411  CL23 HEK-293T C749(1.06)  LDD1615  [23]
 LDCM0412  CL24 HEK-293T C749(1.00)  LDD1616  [23]
 LDCM0413  CL25 HEK-293T C749(0.96)  LDD1617  [23]
 LDCM0414  CL26 HEK-293T C749(0.96)  LDD1618  [23]
 LDCM0415  CL27 HEK-293T C749(0.99)  LDD1619  [23]
 LDCM0416  CL28 HEK-293T C749(0.99)  LDD1620  [23]
 LDCM0417  CL29 HEK-293T C749(1.04)  LDD1621  [23]
 LDCM0418  CL3 HCT 116 C749(1.13)  LDD0735  [10]
 LDCM0419  CL30 HEK-293T C749(0.99)  LDD1623  [23]
 LDCM0420  CL31 HEK-293T C749(1.11)  LDD1624  [23]
 LDCM0421  CL32 HCT 116 C749(1.07)  LDD0738  [10]
 LDCM0422  CL33 HCT 116 C749(1.18)  LDD0739  [10]
 LDCM0423  CL34 HCT 116 C749(0.87)  LDD0740  [10]
 LDCM0424  CL35 HCT 116 C749(0.89)  LDD0741  [10]
 LDCM0425  CL36 HCT 116 C749(0.74)  LDD0742  [10]
 LDCM0426  CL37 HCT 116 C749(0.65)  LDD0743  [10]
 LDCM0428  CL39 HCT 116 C749(0.81)  LDD0745  [10]
 LDCM0429  CL4 HCT 116 C749(1.09)  LDD0746  [10]
 LDCM0430  CL40 HCT 116 C749(0.88)  LDD0747  [10]
 LDCM0431  CL41 HCT 116 C749(0.66)  LDD0748  [10]
 LDCM0432  CL42 HCT 116 C749(0.52)  LDD0749  [10]
 LDCM0433  CL43 HCT 116 C749(0.82)  LDD0750  [10]
 LDCM0434  CL44 HCT 116 C749(0.78)  LDD0751  [10]
 LDCM0435  CL45 HCT 116 C749(0.75)  LDD0752  [10]
 LDCM0436  CL46 HCT 116 C749(0.92)  LDD0753  [10]
 LDCM0437  CL47 HCT 116 C749(0.93)  LDD0754  [10]
 LDCM0438  CL48 HCT 116 C749(0.91)  LDD0755  [10]
 LDCM0439  CL49 HCT 116 C749(0.72)  LDD0756  [10]
 LDCM0440  CL5 HCT 116 C749(0.97)  LDD0757  [10]
 LDCM0441  CL50 HCT 116 C749(0.74)  LDD0758  [10]
 LDCM0442  CL51 HCT 116 C749(0.77)  LDD0759  [10]
 LDCM0443  CL52 HCT 116 C749(1.08)  LDD0760  [10]
 LDCM0444  CL53 HCT 116 C749(1.14)  LDD0761  [10]
 LDCM0445  CL54 HCT 116 C749(1.05)  LDD0762  [10]
 LDCM0446  CL55 HCT 116 C749(0.91)  LDD0763  [10]
 LDCM0447  CL56 HCT 116 C749(0.87)  LDD0764  [10]
 LDCM0448  CL57 HCT 116 C749(0.85)  LDD0765  [10]
 LDCM0449  CL58 HCT 116 C749(1.17)  LDD0766  [10]
 LDCM0450  CL59 HCT 116 C749(0.81)  LDD0767  [10]
 LDCM0451  CL6 HCT 116 C749(1.24)  LDD0768  [10]
 LDCM0452  CL60 HCT 116 C749(0.86)  LDD0769  [10]
 LDCM0453  CL61 HCT 116 C749(1.11)  LDD0770  [10]
 LDCM0454  CL62 HCT 116 C749(1.02)  LDD0771  [10]
 LDCM0455  CL63 HCT 116 C749(0.84)  LDD0772  [10]
 LDCM0456  CL64 HCT 116 C749(0.55)  LDD0773  [10]
 LDCM0457  CL65 HCT 116 C749(0.70)  LDD0774  [10]
 LDCM0458  CL66 HCT 116 C749(0.91)  LDD0775  [10]
 LDCM0459  CL67 HCT 116 C749(0.87)  LDD0776  [10]
 LDCM0460  CL68 HCT 116 C749(0.84)  LDD0777  [10]
 LDCM0461  CL69 HCT 116 C749(1.05)  LDD0778  [10]
 LDCM0462  CL7 HCT 116 C749(1.01)  LDD0779  [10]
 LDCM0463  CL70 HCT 116 C749(0.89)  LDD0780  [10]
 LDCM0464  CL71 HCT 116 C749(0.96)  LDD0781  [10]
 LDCM0465  CL72 HCT 116 C749(0.89)  LDD0782  [10]
 LDCM0466  CL73 HCT 116 C749(0.89)  LDD0783  [10]
 LDCM0467  CL74 HCT 116 C749(0.94)  LDD0784  [10]
 LDCM0469  CL76 HCT 116 C749(0.84)  LDD0786  [10]
 LDCM0470  CL77 HCT 116 C749(0.79)  LDD0787  [10]
 LDCM0471  CL78 HCT 116 C749(0.94)  LDD0788  [10]
 LDCM0472  CL79 HCT 116 C749(0.66)  LDD0789  [10]
 LDCM0473  CL8 HCT 116 C749(1.50)  LDD0790  [10]
 LDCM0474  CL80 HCT 116 C749(0.73)  LDD0791  [10]
 LDCM0475  CL81 HCT 116 C749(0.66)  LDD0792  [10]
 LDCM0476  CL82 HCT 116 C749(0.67)  LDD0793  [10]
 LDCM0477  CL83 HCT 116 C749(0.90)  LDD0794  [10]
 LDCM0478  CL84 HCT 116 C749(1.01)  LDD0795  [10]
 LDCM0479  CL85 HCT 116 C749(1.03)  LDD0796  [10]
 LDCM0480  CL86 HCT 116 C749(0.91)  LDD0797  [10]
 LDCM0481  CL87 HCT 116 C749(0.36)  LDD0798  [10]
 LDCM0482  CL88 HCT 116 C749(0.75)  LDD0799  [10]
 LDCM0483  CL89 HCT 116 C749(0.72)  LDD0800  [10]
 LDCM0484  CL9 HCT 116 C749(1.13)  LDD0801  [10]
 LDCM0485  CL90 HCT 116 C749(0.72)  LDD0802  [10]
 LDCM0486  CL91 HCT 116 C749(0.88)  LDD0803  [10]
 LDCM0487  CL92 HCT 116 C749(1.02)  LDD0804  [10]
 LDCM0488  CL93 HCT 116 C749(0.68)  LDD0805  [10]
 LDCM0489  CL94 HCT 116 C749(0.80)  LDD0806  [10]
 LDCM0490  CL95 HCT 116 C749(0.77)  LDD0807  [10]
 LDCM0491  CL96 HCT 116 C749(0.66)  LDD0808  [10]
 LDCM0492  CL97 HCT 116 C749(0.69)  LDD0809  [10]
 LDCM0493  CL98 HCT 116 C749(0.81)  LDD0810  [10]
 LDCM0494  CL99 HCT 116 C749(0.78)  LDD0811  [10]
 LDCM0495  E2913 HEK-293T C749(1.19)  LDD1698  [23]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C749(1.42)  LDD1702  [6]
 LDCM0625  F8 Ramos C749(1.26)  LDD2187  [24]
 LDCM0572  Fragment10 Ramos C749(3.56)  LDD2189  [24]
 LDCM0573  Fragment11 Ramos C749(0.84)  LDD2190  [24]
 LDCM0574  Fragment12 Ramos C749(0.93)  LDD2191  [24]
 LDCM0575  Fragment13 Ramos C749(0.95)  LDD2192  [24]
 LDCM0576  Fragment14 Ramos C749(0.77)  LDD2193  [24]
 LDCM0579  Fragment20 Ramos C749(0.99)  LDD2194  [24]
 LDCM0580  Fragment21 Ramos C749(0.95)  LDD2195  [24]
 LDCM0582  Fragment23 Ramos C749(1.00)  LDD2196  [24]
 LDCM0578  Fragment27 Ramos C749(1.00)  LDD2197  [24]
 LDCM0586  Fragment28 Ramos C749(0.86)  LDD2198  [24]
 LDCM0588  Fragment30 Ramos C749(1.24)  LDD2199  [24]
 LDCM0589  Fragment31 Ramos C749(0.88)  LDD2200  [24]
 LDCM0590  Fragment32 Ramos C749(3.69)  LDD2201  [24]
 LDCM0468  Fragment33 HCT 116 C749(0.82)  LDD0785  [10]
 LDCM0596  Fragment38 Ramos C749(0.83)  LDD2203  [24]
 LDCM0566  Fragment4 Ramos C749(1.00)  LDD2184  [24]
 LDCM0427  Fragment51 HCT 116 C749(0.89)  LDD0744  [10]
 LDCM0610  Fragment52 Ramos C749(1.47)  LDD2204  [24]
 LDCM0614  Fragment56 Ramos C749(1.22)  LDD2205  [24]
 LDCM0569  Fragment7 Ramos C749(1.21)  LDD2186  [24]
 LDCM0571  Fragment9 Ramos C749(1.49)  LDD2188  [24]
 LDCM0116  HHS-0101 DM93 Y665(0.99)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y665(0.88)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y665(1.54)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y665(0.94)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y665(1.04)  LDD0268  [8]
 LDCM0022  KB02 HEK-293T C749(0.92)  LDD1492  [23]
 LDCM0023  KB03 HEK-293T C749(1.05)  LDD1497  [23]
 LDCM0024  KB05 NCI-H1048 C749(3.45)  LDD3341  [25]
 LDCM0109  NEM HeLa N.A.  LDD0227  [20]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C749(0.36)  LDD2089  [6]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C749(1.26)  LDD2092  [6]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C749(1.08)  LDD2093  [6]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C749(1.87)  LDD2094  [6]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C749(0.66)  LDD2097  [6]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C749(1.12)  LDD2098  [6]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C749(0.65)  LDD2099  [6]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C749(0.81)  LDD2100  [6]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C749(0.82)  LDD2101  [6]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C749(1.10)  LDD2104  [6]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C749(0.71)  LDD2107  [6]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C749(0.42)  LDD2108  [6]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C749(0.73)  LDD2109  [6]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C749(0.80)  LDD2111  [6]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C749(2.03)  LDD2114  [6]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C749(0.52)  LDD2115  [6]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C749(0.32)  LDD2118  [6]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C749(2.88)  LDD2119  [6]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C749(1.02)  LDD2120  [6]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C749(0.94)  LDD2123  [6]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C749(0.22)  LDD2124  [6]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C749(0.68)  LDD2125  [6]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C749(0.87)  LDD2127  [6]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C749(1.19)  LDD2128  [6]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C749(0.74)  LDD2129  [6]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C749(0.34)  LDD2134  [6]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C749(0.78)  LDD2135  [6]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C749(1.01)  LDD2136  [6]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C749(0.96)  LDD2137  [6]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C749(1.38)  LDD1700  [6]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C749(0.53)  LDD2140  [6]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C749(1.11)  LDD2143  [6]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C749(0.81)  LDD2146  [6]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C749(2.88)  LDD2147  [6]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C749(0.32)  LDD2148  [6]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C749(1.63)  LDD2153  [6]
 LDCM0099  Phenelzine HEK-293T 20.00  LDD0382  [5]
 LDCM0112  W16 Hep-G2 C749(0.56)  LDD0239  [11]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
10 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
11 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
12 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
13 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
14 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
15 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
16 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
17 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
18 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
19 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
20 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
21 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
22 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
23 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
24 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
25 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840