Probe Name	Sulforaphane-probe2					Probe Info
Probe concentration	5 uM
Criteria	Quantification: Probe vs (Probe+Competitor)
Quantitative Method	SILAC
Competitor Name	Sulforaphane					Competitor Info
Competitor Concentration	100 uM
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Breast
	Disease Model		Invasive breast carcinoma [ICD-11:2C60]
	Model Name		Human invasive breast carcinoma cells (MCF-7)

Target ID	Target Name					Binding Ratio
LDTP04232	4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1)					6.3
LDTP00225	26S proteasome non-ATPase regulatory subunit 9 (PSMD9)					4.4
LDTP14535	6-phosphogluconolactonase (PGLS)					3.6
LDTP05346	Methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], mitochondrial (ALDH6A1)					3.5
LDTP01647	Probable bifunctional dTTP/UTP pyrophosphatase/methyltransferase protein (ASMTL)					3.2
LDTP00497	Cyclin-G-associated kinase (GAK)					3.0
LDTP06270	Signal peptidase complex subunit 2 (SPCS2)					2.9
LDTP00609	Inositol polyphosphate 4-phosphatase type II (INPP4B)					2.9
LDTP04435	Interleukin-1 receptor-associated kinase 1 (IRAK1)					2.8
LDTP00598	Serine palmitoyltransferase 2 (SPTLC2)					2.4
LDTP02770	Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1)					2.3
LDTP02708	17-beta-hydroxysteroid dehydrogenase type 1 (HSD17B1)					2.3
LDTP05136	DNA-dependent protein kinase catalytic subunit (PRKDC)					2.2
LDTP06098	E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25)					2.1
LDTP01220	Mitochondrial-processing peptidase subunit beta (PMPCB)					2.1
LDTP00527	Phosphoribosylformylglycinamidine synthase (PFAS)					2.1
LDTP03946	Glycine--tRNA ligase (GARS1)					2.1

Target ID	Target Name					Binding Ratio
LDTP16811	Ran-binding protein 6 (RANBP6)					4.2
LDTP04071	Voltage-dependent anion-selective channel protein 2 (VDAC2)					3.3
LDTP05511	Cytoskeleton-associated protein 4 (CKAP4)					2.5
LDTP00266	Importin-5 (IPO5)					2.0

Target ID	Target Name					Binding Ratio
LDTP03969	Signal transducer and activator of transcription 1-alpha/beta (STAT1)					3.2
LDTP03903	Signal transducer and activator of transcription 3 (STAT3)					3.1
LDTP01191	Zinc finger protein 217 (ZNF217)					2.9
LDTP01414	Metastasis-associated protein MTA2 (MTA2)					2.3

Target ID	Target Name					Binding Ratio
LDTP00239	DNA fragmentation factor subunit alpha (DFFA)					4.4
LDTP04396	RNA-binding protein FXR2 (FXR2)					4.1
LDTP06281	Condensin complex subunit 1 (NCAPD2)					3.6
LDTP01021	Perilipin-3 (PLIN3)					3.4
LDTP00202	AH receptor-interacting protein (AIP)					3.4
LDTP01567	Structural maintenance of chromosomes protein 2 (SMC2)					3.2
LDTP04092	Crk-like protein (CRKL)					3.2
LDTP04483	Hepatoma-derived growth factor (HDGF)					3.2
LDTP04722	BH3-interacting domain death agonist (BID)					3.0
LDTP01750	Peroxisomal membrane protein 11B (PEX11B)					3.0
LDTP01522	Reticulon-3 (RTN3)					3.0
LDTP06439	Thyroid receptor-interacting protein 6 (TRIP6)					2.9
LDTP09363	TBC1 domain family member 15 (TBC1D15)					2.8
LDTP03967	Lamina-associated polypeptide 2, isoform alpha (TMPO)					2.8
LDTP01684	BAG family molecular chaperone regulator 3 (BAG3)					2.7
LDTP00019	Shootin-1 (SHTN1)					2.7
LDTP06310	Early endosome antigen 1 (EEA1)					2.7
LDTP04514	Heterogeneous nuclear ribonucleoprotein F (HNRNPF)					2.7
LDTP04594	Protein transport protein Sec24C (SEC24C)					2.7
LDTP08573	Ankyrin repeat and KH domain-containing protein 1 (ANKHD1)					2.3
LDTP06367	Poly(rC)-binding protein 2 (PCBP2)					2.2
LDTP05259	Heterogeneous nuclear ribonucleoprotein U (HNRNPU)					2.2
LDTP07605	La-related protein 1 (LARP1)					2.1
LDTP07903	La-related protein 4 (LARP4)					2.0

1	Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane. Chem Commun (Camb). 2017 May 4;53(37):5182-5185. doi: 10.1039/c6cc08797c. Mass spectrometry data entry: PXD006279