Details of the Target

General Information of Target

Target ID LDTP15311
Target Name Deaminated glutathione amidase (NIT1)
Gene Name NIT1
Gene ID 4817
Synonyms
Deaminated glutathione amidase; dGSH amidase; EC 3.5.1.128; Nitrilase homolog 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDLMNGQASSVNIAATASEKSSSSESLSDKGSELKKSFDAVVFDVLKVTPEEYAGQITLM
DVPVFKAIQPDELSSCGWNKKEKYSSAPNAVAFTRRFNHVSFWVVREILHAQTLKIRAEV
LSHYIKTAKKLYELNNLHALMAVVSGLQSAPIFRLTKTWALLSRKDKTTFEKLEYVMSKE
DNYKRLRDYISSLKMTPCIPYLGIYLSDLTYIDSAYPSTGSILENEQRSNLMNNILRIIS
DLQQSCEYDIPMLPHVQKYLNSVQYIEELQKFVEDDNYKLSLKIEPGTSTPRSAASREDL
VGPEVGASPQSGRKSVAAEGALLPQTPPSPRNLIPHGHRKCHSLGYNFIHKMNTAEFKSA
TFPNAGPRHLLDDSVMEPHAPSRGQAESSTLSSGISIGSSDGSELSEETSWPAFERNRLY
HSLGPVTRVARNGYRSHMKASSSAESEDLAVHLYPGAVTIQGVLRRKTLLKEGKKPTVAS
WTKYWAALCGTQLFYYAAKSLKATERKHFKSTSNKNVSVIGWMVMMADDPEHPDLFLLTD
SEKGNSYKFQAGNRMNAMLWFKHLSAACQSNKQQVPTNLMTFE
Target Bioclass
Enzyme
Family
Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family
Subcellular location
Cytoplasm; Mitochondrion
Function
Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.
Uniprot ID
Q86X76
Ensemble ID
ENST00000368007.8
HGNC ID
HGNC:7828
ChEMBL ID
CHEMBL4295883

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
BTD
 Probe Info  		C67(1.13)  LDD2099  [2]
AHL-Pu-1
 Probe Info  		C176(2.16)  LDD0170  [3]
DBIA
 Probe Info  		C165(34.34)  LDD0209  [4]
Acrolein
 Probe Info  		N.A.  LDD0222  [5]
5E-2FA
 Probe Info  		H100(0.00); H324(0.00)  LDD2235  [6]
4-Iodoacetamidophenylacetylene
 Probe Info  		C120(0.00); C203(0.00); C145(0.00); C80(0.00)  LDD0038  [7]
IA-alkyne
 Probe Info  		C259(0.00); C251(0.00); C285(0.00); C292(0.00)  LDD0036  [7]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [8]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [8]
Lodoacetamide azide
 Probe Info  		C120(0.00); C203(0.00); C145(0.00); C80(0.00)  LDD0037  [7]
TFBX
 Probe Info  		N.A.  LDD0027  [9]
IPM
 Probe Info  		N.A.  LDD0005  [10]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [11]
AOyne
 Probe Info  		14.10  LDD0443  [12]
NAIA_5
 Probe Info  		C120(0.00); C145(0.00); C80(0.00); C67(0.00)  LDD2223  [13]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [14]
Photonaproxen
 Probe Info  		N.A.  LDD0157  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C67(0.93)  LDD2117  [2]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C67(1.18)  LDD2152  [2]
 LDCM0025  4SU-RNA DM93 C176(2.16)  LDD0170  [3]
 LDCM0214  AC1 HEK-293T C67(1.03); C203(0.99); C165(1.20); C285(1.22)  LDD1507  [16]
 LDCM0215  AC10 HEK-293T C67(0.98); C203(0.96); C120(0.98); C165(1.13)  LDD1508  [16]
 LDCM0226  AC11 HEK-293T C67(0.96); C203(1.05); C165(0.97); C285(1.11)  LDD1509  [16]
 LDCM0237  AC12 HEK-293T C203(1.07); C165(1.05)  LDD1510  [16]
 LDCM0259  AC14 HEK-293T C203(0.98); C165(1.00); C259(0.85)  LDD1512  [16]
 LDCM0270  AC15 HEK-293T C203(1.05); C165(0.98); C292(1.17); C259(1.07)  LDD1513  [16]
 LDCM0276  AC17 HEK-293T C67(0.99); C203(1.23); C165(1.42); C285(1.04)  LDD1515  [16]
 LDCM0277  AC18 HEK-293T C67(0.95); C203(0.96); C120(0.97); C165(1.09)  LDD1516  [16]
 LDCM0278  AC19 HEK-293T C67(0.61); C203(1.10); C165(1.25); C285(0.79)  LDD1517  [16]
 LDCM0279  AC2 HEK-293T C67(1.02); C203(0.98); C120(1.16); C165(0.82)  LDD1518  [16]
 LDCM0280  AC20 HEK-293T C203(1.09); C165(1.13)  LDD1519  [16]
 LDCM0281  AC21 HEK-293T C203(1.05); C285(0.97)  LDD1520  [16]
 LDCM0282  AC22 HEK-293T C203(1.16); C165(1.08); C259(0.86)  LDD1521  [16]
 LDCM0283  AC23 HEK-293T C203(1.16); C165(1.26); C292(1.08); C259(1.03)  LDD1522  [16]
 LDCM0284  AC24 HEK-293T C67(1.04); C203(1.06); C165(1.07); C285(0.96)  LDD1523  [16]
 LDCM0285  AC25 HEK-293T C67(0.91); C203(1.05); C165(1.30); C285(0.98)  LDD1524  [16]
 LDCM0286  AC26 HEK-293T C67(1.00); C203(0.91); C120(1.00); C165(0.98)  LDD1525  [16]
 LDCM0287  AC27 HEK-293T C67(0.98); C203(0.94); C165(0.88); C285(0.92)  LDD1526  [16]
 LDCM0288  AC28 HEK-293T C203(0.95); C165(0.94)  LDD1527  [16]
 LDCM0289  AC29 HEK-293T C203(0.89); C285(0.99)  LDD1528  [16]
 LDCM0290  AC3 HEK-293T C67(0.96); C203(0.98); C165(0.97); C285(0.93)  LDD1529  [16]
 LDCM0291  AC30 HEK-293T C203(0.91); C165(0.85); C259(0.91)  LDD1530  [16]
 LDCM0292  AC31 HEK-293T C203(0.91); C165(0.98); C292(1.14); C259(0.96)  LDD1531  [16]
 LDCM0293  AC32 HEK-293T C67(0.94); C203(0.98); C165(1.11); C285(1.03)  LDD1532  [16]
 LDCM0294  AC33 HEK-293T C67(0.83); C203(1.45); C165(1.87); C285(1.00)  LDD1533  [16]
 LDCM0295  AC34 HEK-293T C67(1.00); C203(1.14); C120(1.15); C165(1.13)  LDD1534  [16]
 LDCM0296  AC35 HEK-293T C67(0.91); C203(1.30); C165(1.17); C285(0.94)  LDD1535  [16]
 LDCM0297  AC36 HEK-293T C203(1.17); C165(1.13)  LDD1536  [16]
 LDCM0298  AC37 HEK-293T C203(1.01); C285(1.02)  LDD1537  [16]
 LDCM0299  AC38 HEK-293T C203(1.20); C165(1.20); C259(0.91)  LDD1538  [16]
 LDCM0300  AC39 HEK-293T C203(1.14); C165(1.29); C292(1.16); C259(0.92)  LDD1539  [16]
 LDCM0301  AC4 HEK-293T C203(0.97); C165(1.00)  LDD1540  [16]
 LDCM0302  AC40 HEK-293T C67(0.96); C203(1.02); C165(1.10); C285(1.06)  LDD1541  [16]
 LDCM0303  AC41 HEK-293T C67(1.00); C203(1.36); C165(1.76); C285(1.15)  LDD1542  [16]
 LDCM0304  AC42 HEK-293T C67(0.99); C203(0.96); C120(0.87); C165(1.31)  LDD1543  [16]
 LDCM0305  AC43 HEK-293T C67(0.89); C203(1.17); C165(1.25); C285(1.01)  LDD1544  [16]
 LDCM0306  AC44 HEK-293T C203(1.17); C165(1.04)  LDD1545  [16]
 LDCM0307  AC45 HEK-293T C203(1.12); C285(0.98)  LDD1546  [16]
 LDCM0308  AC46 HEK-293T C203(1.07); C165(1.02); C259(0.86)  LDD1547  [16]
 LDCM0309  AC47 HEK-293T C203(1.07); C165(1.20); C292(1.12); C259(1.04)  LDD1548  [16]
 LDCM0310  AC48 HEK-293T C67(0.88); C203(1.06); C165(1.24); C285(1.12)  LDD1549  [16]
 LDCM0311  AC49 HEK-293T C67(1.00); C203(1.12); C165(1.30); C285(1.10)  LDD1550  [16]
 LDCM0312  AC5 HEK-293T C203(0.92); C285(0.93)  LDD1551  [16]
 LDCM0313  AC50 HEK-293T C67(1.04); C203(0.95); C120(1.08); C165(1.07)  LDD1552  [16]
 LDCM0314  AC51 HEK-293T C67(0.99); C203(1.06); C165(1.04); C285(1.10)  LDD1553  [16]
 LDCM0315  AC52 HEK-293T C203(1.09); C165(1.06)  LDD1554  [16]
 LDCM0316  AC53 HEK-293T C203(1.23); C285(1.05)  LDD1555  [16]
 LDCM0317  AC54 HEK-293T C203(1.18); C165(1.12); C259(0.89)  LDD1556  [16]
 LDCM0318  AC55 HEK-293T C203(1.20); C165(1.46); C292(1.00); C259(0.96)  LDD1557  [16]
 LDCM0319  AC56 HEK-293T C67(1.03); C203(1.04); C165(1.18); C285(1.04)  LDD1558  [16]
 LDCM0320  AC57 HEK-293T C67(0.94); C203(1.15); C165(1.33); C285(1.17)  LDD1559  [16]
 LDCM0321  AC58 HEK-293T C67(0.91); C203(1.10); C120(1.15); C165(1.20)  LDD1560  [16]
 LDCM0322  AC59 HEK-293T C67(0.98); C203(1.02); C165(0.91); C285(0.92)  LDD1561  [16]
 LDCM0323  AC6 HEK-293T C203(0.84); C165(0.89); C259(0.94)  LDD1562  [16]
 LDCM0324  AC60 HEK-293T C203(1.03); C165(1.15)  LDD1563  [16]
 LDCM0325  AC61 HEK-293T C203(1.05); C285(0.91)  LDD1564  [16]
 LDCM0326  AC62 HEK-293T C203(1.06); C165(1.03); C259(0.85)  LDD1565  [16]
 LDCM0327  AC63 HEK-293T C203(1.08); C165(1.20); C292(1.11); C259(1.03)  LDD1566  [16]
 LDCM0328  AC64 HEK-293T C67(0.95); C203(0.94); C165(1.03); C285(0.92)  LDD1567  [16]
 LDCM0334  AC7 HEK-293T C203(1.02); C165(0.89); C292(1.16); C259(1.00)  LDD1568  [16]
 LDCM0345  AC8 HEK-293T C67(1.02); C203(0.89); C165(0.92); C285(1.00)  LDD1569  [16]
 LDCM0248  AKOS034007472 HEK-293T C203(1.17); C285(1.10)  LDD1511  [16]
 LDCM0356  AKOS034007680 HEK-293T C67(0.99); C203(1.10); C165(1.20); C285(0.96)  LDD1570  [16]
 LDCM0275  AKOS034007705 HEK-293T C67(1.02); C203(1.00); C165(1.08); C285(1.06)  LDD1514  [16]
 LDCM0156  Aniline NCI-H1299 13.47  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [5]
 LDCM0367  CL1 HEK-293T C67(0.85); C203(0.96); C165(1.02)  LDD1571  [16]
 LDCM0368  CL10 HEK-293T C203(5.80); C165(2.71); C259(0.84)  LDD1572  [16]
 LDCM0369  CL100 HEK-293T C67(1.14); C203(1.03); C165(1.06)  LDD1573  [16]
 LDCM0370  CL101 HEK-293T C67(0.89); C203(1.08); C165(1.08)  LDD1574  [16]
 LDCM0371  CL102 HEK-293T C67(1.00); C203(1.75); C165(1.37); C285(1.09)  LDD1575  [16]
 LDCM0372  CL103 HEK-293T C67(0.96); C203(1.38); C165(1.36); C292(1.10)  LDD1576  [16]
 LDCM0373  CL104 HEK-293T C67(1.11); C203(0.87); C165(0.87)  LDD1577  [16]
 LDCM0374  CL105 HEK-293T C67(0.94); C203(1.08); C165(1.16)  LDD1578  [16]
 LDCM0375  CL106 HEK-293T C67(0.99); C203(1.17); C165(1.11); C285(1.06)  LDD1579  [16]
 LDCM0376  CL107 HEK-293T C67(0.87); C203(1.18); C165(1.21); C292(1.13)  LDD1580  [16]
 LDCM0377  CL108 HEK-293T C67(1.02); C203(0.98); C165(1.13)  LDD1581  [16]
 LDCM0378  CL109 HEK-293T C67(1.02); C203(0.88); C165(0.98)  LDD1582  [16]
 LDCM0379  CL11 HEK-293T C203(1.43); C165(1.29); C292(0.85); C259(1.10)  LDD1583  [16]
 LDCM0380  CL110 HEK-293T C67(1.01); C203(1.80); C165(1.90); C285(1.09)  LDD1584  [16]
 LDCM0381  CL111 HEK-293T C67(0.90); C203(3.28); C165(0.82); C292(1.01)  LDD1585  [16]
 LDCM0382  CL112 HEK-293T C67(1.09); C203(1.20); C165(1.39)  LDD1586  [16]
 LDCM0383  CL113 HEK-293T C67(0.90); C203(1.11); C165(1.17)  LDD1587  [16]
 LDCM0384  CL114 HEK-293T C67(1.11); C203(0.93); C165(1.12); C285(1.13)  LDD1588  [16]
 LDCM0385  CL115 HEK-293T C67(0.95); C203(0.97); C165(1.00); C292(0.94)  LDD1589  [16]
 LDCM0386  CL116 HEK-293T C67(1.02); C203(1.25); C165(1.33)  LDD1590  [16]
 LDCM0387  CL117 HEK-293T C67(0.97); C203(1.15); C165(1.21)  LDD1591  [16]
 LDCM0388  CL118 HEK-293T C67(0.88); C203(0.99); C165(0.98); C285(1.11)  LDD1592  [16]
 LDCM0389  CL119 HEK-293T C67(0.89); C203(0.98); C165(1.02); C292(1.13)  LDD1593  [16]
 LDCM0390  CL12 HEK-293T C67(0.93); C203(1.47); C165(1.24); C285(1.04)  LDD1594  [16]
 LDCM0391  CL120 HEK-293T C67(1.15); C203(1.14); C165(1.28)  LDD1595  [16]
 LDCM0392  CL121 HEK-293T C67(0.93); C203(1.26); C165(1.31)  LDD1596  [16]
 LDCM0393  CL122 HEK-293T C67(1.08); C203(1.20); C165(1.20); C285(1.07)  LDD1597  [16]
 LDCM0394  CL123 HEK-293T C67(0.94); C203(1.27); C165(1.39); C292(1.12)  LDD1598  [16]
 LDCM0395  CL124 HEK-293T C67(1.09); C203(1.09); C165(1.03)  LDD1599  [16]
 LDCM0396  CL125 HEK-293T C67(0.89); C203(1.04); C165(1.00)  LDD1600  [16]
 LDCM0397  CL126 HEK-293T C67(0.91); C203(1.11); C165(1.27); C285(1.04)  LDD1601  [16]
 LDCM0398  CL127 HEK-293T C67(0.91); C203(1.39); C165(1.38); C292(0.86)  LDD1602  [16]
 LDCM0399  CL128 HEK-293T C67(1.03); C203(1.06); C165(1.11)  LDD1603  [16]
 LDCM0400  CL13 HEK-293T C67(0.81); C203(1.29); C165(1.07)  LDD1604  [16]
 LDCM0401  CL14 HEK-293T C67(0.84); C203(1.02); C165(1.18); C285(1.07)  LDD1605  [16]
 LDCM0402  CL15 HEK-293T C67(1.02); C203(3.20); C165(2.00); C292(1.27)  LDD1606  [16]
 LDCM0403  CL16 HEK-293T C67(1.04); C203(0.94); C165(1.15)  LDD1607  [16]
 LDCM0404  CL17 HEK-293T C67(0.95); C203(1.48); C165(1.93); C285(1.38)  LDD1608  [16]
 LDCM0405  CL18 HEK-293T C67(0.96); C203(1.12); C120(0.96); C165(1.14)  LDD1609  [16]
 LDCM0406  CL19 HEK-293T C67(0.98); C203(1.55); C165(1.26); C285(1.08)  LDD1610  [16]
 LDCM0407  CL2 HEK-293T C67(0.92); C203(1.00); C165(1.14); C285(1.06)  LDD1611  [16]
 LDCM0408  CL20 HEK-293T C203(1.22); C165(1.03)  LDD1612  [16]
 LDCM0409  CL21 HEK-293T C203(1.53); C285(0.85)  LDD1613  [16]
 LDCM0410  CL22 HEK-293T C203(1.44); C165(1.34); C259(0.92)  LDD1614  [16]
 LDCM0411  CL23 HEK-293T C203(1.49); C165(1.34); C292(1.31); C259(1.07)  LDD1615  [16]
 LDCM0412  CL24 HEK-293T C67(1.10); C203(1.26); C165(1.17); C285(1.14)  LDD1616  [16]
 LDCM0413  CL25 HEK-293T C67(0.90); C203(1.15); C165(1.07)  LDD1617  [16]
 LDCM0414  CL26 HEK-293T C67(0.99); C203(1.30); C165(1.40); C285(0.99)  LDD1618  [16]
 LDCM0415  CL27 HEK-293T C67(0.97); C203(1.26); C165(1.26); C292(1.06)  LDD1619  [16]
 LDCM0416  CL28 HEK-293T C67(1.12); C203(1.21); C165(1.12)  LDD1620  [16]
 LDCM0417  CL29 HEK-293T C67(0.99); C203(1.27); C165(1.37); C285(1.14)  LDD1621  [16]
 LDCM0418  CL3 HEK-293T C67(1.07); C203(1.08); C165(1.06); C292(1.07)  LDD1622  [16]
 LDCM0419  CL30 HEK-293T C67(1.00); C203(1.09); C120(1.24); C165(1.18)  LDD1623  [16]
 LDCM0420  CL31 HEK-293T C67(0.92); C203(1.09); C165(1.25); C285(1.02)  LDD1624  [16]
 LDCM0421  CL32 HEK-293T C203(1.17); C165(1.07)  LDD1625  [16]
 LDCM0422  CL33 HEK-293T C203(2.17); C285(0.85)  LDD1626  [16]
 LDCM0423  CL34 HEK-293T C203(1.30); C165(1.33); C259(0.91)  LDD1627  [16]
 LDCM0424  CL35 HEK-293T C203(2.28); C165(1.35); C292(1.26); C259(1.05)  LDD1628  [16]
 LDCM0425  CL36 HEK-293T C67(1.04); C203(1.41); C165(1.31); C285(1.16)  LDD1629  [16]
 LDCM0426  CL37 HEK-293T C67(0.83); C203(2.03); C165(1.72)  LDD1630  [16]
 LDCM0428  CL39 HEK-293T C67(0.76); C203(1.32); C165(1.47); C292(1.00)  LDD1632  [16]
 LDCM0429  CL4 HEK-293T C67(0.97); C203(0.95); C165(1.04)  LDD1633  [16]
 LDCM0430  CL40 HEK-293T C67(0.98); C203(1.44); C165(1.44)  LDD1634  [16]
 LDCM0431  CL41 HEK-293T C67(1.09); C203(1.15); C165(1.82); C285(1.12)  LDD1635  [16]
 LDCM0432  CL42 HEK-293T C67(0.91); C203(1.22); C120(1.11); C165(1.07)  LDD1636  [16]
 LDCM0433  CL43 HEK-293T C67(1.05); C203(1.12); C165(0.89); C285(0.98)  LDD1637  [16]
 LDCM0434  CL44 HEK-293T C203(1.23); C165(1.11)  LDD1638  [16]
 LDCM0435  CL45 HEK-293T C203(2.63); C285(0.79)  LDD1639  [16]
 LDCM0436  CL46 HEK-293T C203(1.38); C165(1.11); C259(0.92)  LDD1640  [16]
 LDCM0437  CL47 HEK-293T C203(1.43); C165(1.32); C292(1.41); C259(1.06)  LDD1641  [16]
 LDCM0438  CL48 HEK-293T C67(0.90); C203(1.67); C165(1.62); C285(1.06)  LDD1642  [16]
 LDCM0439  CL49 HEK-293T C67(1.03); C203(1.17); C165(1.04)  LDD1643  [16]
 LDCM0440  CL5 HEK-293T C67(1.02); C203(1.15); C165(1.34); C285(1.05)  LDD1644  [16]
 LDCM0441  CL50 HEK-293T C67(0.89); C203(1.29); C165(1.44); C285(1.06)  LDD1645  [16]
 LDCM0443  CL52 HEK-293T C67(0.98); C203(1.05); C165(1.12)  LDD1646  [16]
 LDCM0444  CL53 HEK-293T C67(0.98); C203(1.16); C165(1.32); C285(1.10)  LDD1647  [16]
 LDCM0445  CL54 HEK-293T C67(0.96); C203(1.13); C120(0.93); C165(1.49)  LDD1648  [16]
 LDCM0446  CL55 HEK-293T C67(0.95); C203(1.35); C165(1.12); C285(1.00)  LDD1649  [16]
 LDCM0447  CL56 HEK-293T C203(1.26); C165(1.09)  LDD1650  [16]
 LDCM0448  CL57 HEK-293T C203(4.10); C285(0.78)  LDD1651  [16]
 LDCM0449  CL58 HEK-293T C203(1.48); C165(1.32); C259(0.84)  LDD1652  [16]
 LDCM0450  CL59 HEK-293T C203(1.55); C165(1.59); C292(1.20); C259(1.04)  LDD1653  [16]
 LDCM0451  CL6 HEK-293T C67(0.95); C203(1.00); C120(1.09); C165(1.37)  LDD1654  [16]
 LDCM0452  CL60 HEK-293T C67(0.85); C203(1.49); C165(1.54); C285(1.19)  LDD1655  [16]
 LDCM0453  CL61 HEK-293T C67(1.01); C203(1.21); C165(1.23)  LDD1656  [16]
 LDCM0454  CL62 HEK-293T C67(0.91); C203(1.20); C165(1.19); C285(1.07)  LDD1657  [16]
 LDCM0455  CL63 HEK-293T C67(0.87); C203(1.30); C165(1.26); C292(1.05)  LDD1658  [16]
 LDCM0456  CL64 HEK-293T C67(1.29); C203(1.18); C165(1.34)  LDD1659  [16]
 LDCM0457  CL65 HEK-293T C67(0.95); C203(1.31); C165(1.48); C285(1.21)  LDD1660  [16]
 LDCM0458  CL66 HEK-293T C67(0.93); C203(1.30); C120(0.97); C165(1.32)  LDD1661  [16]
 LDCM0459  CL67 HEK-293T C67(0.93); C203(2.12); C165(1.47); C285(1.12)  LDD1662  [16]
 LDCM0460  CL68 HEK-293T C203(1.32); C165(1.10)  LDD1663  [16]
 LDCM0461  CL69 HEK-293T C203(1.70); C285(0.94)  LDD1664  [16]
 LDCM0462  CL7 HEK-293T C67(0.90); C203(1.20); C165(0.98); C285(1.04)  LDD1665  [16]
 LDCM0463  CL70 HEK-293T C203(1.47); C165(1.41); C259(0.85)  LDD1666  [16]
 LDCM0464  CL71 HEK-293T C203(1.36); C165(1.14); C292(1.06); C259(0.97)  LDD1667  [16]
 LDCM0465  CL72 HEK-293T C67(0.99); C203(1.71); C165(1.70); C285(1.20)  LDD1668  [16]
 LDCM0466  CL73 HEK-293T C67(1.00); C203(2.78); C165(1.19)  LDD1669  [16]
 LDCM0467  CL74 HEK-293T C67(0.91); C203(0.98); C165(0.96); C285(1.02)  LDD1670  [16]
 LDCM0469  CL76 HEK-293T C67(1.04); C203(0.92); C165(1.04)  LDD1672  [16]
 LDCM0470  CL77 HEK-293T C67(0.85); C203(2.35); C165(2.61); C285(1.12)  LDD1673  [16]
 LDCM0471  CL78 HEK-293T C67(0.92); C203(0.98); C120(1.14); C165(0.96)  LDD1674  [16]
 LDCM0472  CL79 HEK-293T C67(0.96); C203(1.03); C165(1.01); C285(0.93)  LDD1675  [16]
 LDCM0473  CL8 HEK-293T C203(1.21); C165(1.50)  LDD1676  [16]
 LDCM0474  CL80 HEK-293T C203(1.10); C165(0.98)  LDD1677  [16]
 LDCM0475  CL81 HEK-293T C203(1.63); C285(0.94)  LDD1678  [16]
 LDCM0476  CL82 HEK-293T C203(1.22); C165(1.00); C259(0.97)  LDD1679  [16]
 LDCM0477  CL83 HEK-293T C203(1.22); C165(1.10); C292(1.09); C259(0.99)  LDD1680  [16]
 LDCM0478  CL84 HEK-293T C67(0.92); C203(1.28); C165(1.25); C285(1.11)  LDD1681  [16]
 LDCM0479  CL85 HEK-293T C67(0.91); C203(1.27); C165(1.22)  LDD1682  [16]
 LDCM0480  CL86 HEK-293T C67(0.92); C203(1.10); C165(1.21); C285(0.96)  LDD1683  [16]
 LDCM0481  CL87 HEK-293T C67(0.80); C203(1.07); C165(1.13); C292(1.07)  LDD1684  [16]
 LDCM0482  CL88 HEK-293T C67(1.02); C203(1.58); C165(1.39)  LDD1685  [16]
 LDCM0483  CL89 HEK-293T C67(0.97); C203(1.33); C165(1.40); C285(1.16)  LDD1686  [16]
 LDCM0484  CL9 HEK-293T C203(1.12); C285(0.93)  LDD1687  [16]
 LDCM0485  CL90 HEK-293T C67(0.84); C203(1.19); C120(0.85); C165(1.23)  LDD1688  [16]
 LDCM0486  CL91 HEK-293T C67(1.03); C203(1.06); C165(1.08); C285(0.91)  LDD1689  [16]
 LDCM0487  CL92 HEK-293T C203(1.37); C165(1.44)  LDD1690  [16]
 LDCM0488  CL93 HEK-293T C203(1.51); C285(0.89)  LDD1691  [16]
 LDCM0489  CL94 HEK-293T C203(1.40); C165(1.40); C259(0.88)  LDD1692  [16]
 LDCM0490  CL95 HEK-293T C203(1.29); C165(1.33); C292(1.15); C259(0.98)  LDD1693  [16]
 LDCM0491  CL96 HEK-293T C67(0.93); C203(1.27); C165(1.26); C285(1.02)  LDD1694  [16]
 LDCM0492  CL97 HEK-293T C67(0.91); C203(0.92); C165(1.02)  LDD1695  [16]
 LDCM0493  CL98 HEK-293T C67(1.01); C203(0.89); C165(1.12); C285(1.12)  LDD1696  [16]
 LDCM0494  CL99 HEK-293T C67(0.80); C203(1.28); C165(1.56); C292(0.81)  LDD1697  [16]
 LDCM0495  E2913 HEK-293T C67(0.83); C203(1.77); C165(2.43); C292(1.02)  LDD1698  [16]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C17(2.80)  LDD1702  [2]
 LDCM0201  EV-3 T cell C203(4.01)  LDD0515  [17]
 LDCM0625  F8 Ramos C67(0.79); C203(2.15)  LDD2187  [18]
 LDCM0572  Fragment10 Ramos C67(0.71)  LDD2189  [18]
 LDCM0573  Fragment11 MDA-MB-231 C203(2.77)  LDD1391  [19]
 LDCM0574  Fragment12 MDA-MB-231 C203(3.93)  LDD1468  [19]
 LDCM0575  Fragment13 Ramos C67(0.94); 1.81  LDD2192  [18]
 LDCM0576  Fragment14 MDA-MB-231 C203(1.05)  LDD1397  [19]
 LDCM0579  Fragment20 Ramos C67(0.50)  LDD2194  [18]
 LDCM0580  Fragment21 Ramos C203(0.86)  LDD1405  [19]
 LDCM0582  Fragment23 Ramos C203(20.00)  LDD1409  [19]
 LDCM0578  Fragment27 MDA-MB-231 C203(0.84)  LDD1401  [19]
 LDCM0586  Fragment28 MDA-MB-231 C203(1.27)  LDD1415  [19]
 LDCM0588  Fragment30 MDA-MB-231 C203(0.83)  LDD1419  [19]
 LDCM0589  Fragment31 Ramos C67(1.10)  LDD2200  [18]
 LDCM0590  Fragment32 Ramos C203(3.96)  LDD1424  [19]
 LDCM0468  Fragment33 Ramos C203(0.62)  LDD1426  [19]
 LDCM0596  Fragment38 MDA-MB-231 C203(1.48)  LDD1433  [19]
 LDCM0566  Fragment4 MDA-MB-231 C203(0.67); C165(0.86)  LDD1461  [19]
 LDCM0599  Fragment41 MDA-MB-231 C203(8.66)  LDD1438  [19]
 LDCM0601  Fragment43 Ramos C203(2.41)  LDD1442  [19]
 LDCM0603  Fragment45 MDA-MB-231 C203(20.00)  LDD1444  [19]
 LDCM0604  Fragment46 MDA-MB-231 C203(0.99)  LDD1445  [19]
 LDCM0605  Fragment47 MDA-MB-231 C203(0.64)  LDD1446  [19]
 LDCM0606  Fragment48 MDA-MB-231 C203(0.80)  LDD1447  [19]
 LDCM0427  Fragment51 HEK-293T C67(0.92); C203(1.21); C165(1.42); C285(0.96)  LDD1631  [16]
 LDCM0610  Fragment52 Ramos C67(0.98); 1.60  LDD2204  [18]
 LDCM0611  Fragment53 MDA-MB-231 C203(0.79)  LDD1454  [19]
 LDCM0614  Fragment56 MDA-MB-231 C203(0.99)  LDD1458  [19]
 LDCM0569  Fragment7 Ramos C67(0.65); C203(0.70); C80(0.94)  LDD2186  [18]
 LDCM0571  Fragment9 Ramos C203(20.00); C165(20.00)  LDD1464  [19]
 LDCM0022  KB02 MDA-MB-231 C203(2.87)  LDD1374  [19]
 LDCM0023  KB03 Jurkat C165(34.34)  LDD0209  [4]
 LDCM0024  KB05 COLO792 C84(1.29); C97(0.94)  LDD3310  [20]
 LDCM0109  NEM HeLa N.A.  LDD0223  [5]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C67(1.13)  LDD2099  [2]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C67(0.68)  LDD2100  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C67(1.09)  LDD2107  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C67(0.67)  LDD2109  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C67(1.01)  LDD2111  [2]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C67(0.96)  LDD2123  [2]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C67(0.88)  LDD2124  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C67(0.91)  LDD2125  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C67(1.06)  LDD2127  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C67(0.99)  LDD2137  [2]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C67(1.77)  LDD2144  [2]
 LDCM0627  NUDT7-COV-1 HEK-293T C203(0.60)  LDD2206  [21]
 LDCM0628  OTUB2-COV-1 HEK-293T C44(0.79); C203(0.07)  LDD2207  [21]
 LDCM0131  RA190 MM1.R C165(3.15); C176(3.15)  LDD0304  [22]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
9 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
12 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
13 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
14 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
15 Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J Am Chem Soc. 2018 Mar 28;140(12):4259-4268. doi: 10.1021/jacs.7b11639. Epub 2018 Mar 15.
Mass spectrometry data entry: PXD007094
16 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
17 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
18 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
19 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
20 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
21 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
22 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.