Details of the Target

General Information of Target

Target ID LDTP02816
Target Name Replication protein A 32 kDa subunit (RPA2)
Gene Name RPA2
Gene ID 6118
Synonyms
REPA2; RPA32; RPA34; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLV
DEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVV
PPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGR
APISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVS
SIKQAVDFLSNEGHIYSTVDDDHFKSTDAE
Target Bioclass
Other
Family
Replication factor A protein 2 family
Subcellular location
Nucleus
Function
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Uniprot ID
P15927
Ensemble ID
ENST00000313433.11
HGNC ID
HGNC:10290

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A431 SNV: p.E70K DBIA    Probe Info 
DU145 SNV: p.E252D DBIA    Probe Info 
ETK1 SNV: p.I183M DBIA    Probe Info 
HUCCT1 SNV: p.H158R DBIA    Probe Info 
IM95 SNV: p.I242T DBIA    Probe Info 
LS180 SNV: p.G204D DBIA    Probe Info 
SSP25 SNV: p.I183M DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 23 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K139(8.94); K231(6.75); K235(7.57); K85(2.04)  LDD0277  [1]
Probe 1
 Probe Info  		Y92(6.64)  LDD3495  [2]
m-APA
 Probe Info  		11.76  LDD0403  [3]
DBIA
 Probe Info  		C307(1.10)  LDD3311  [4]
AHL-Pu-1
 Probe Info  		C219(4.32)  LDD0168  [5]
HHS-465
 Probe Info  		Y256(10.00)  LDD2237  [6]
5E-2FA
 Probe Info  		N.A.  LDD2235  [7]
ATP probe
 Probe Info  		N.A.  LDD0199  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		C219(0.00); C49(0.00)  LDD0038  [9]
IA-alkyne
 Probe Info  		C219(0.00); C49(0.00)  LDD0036  [9]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [10]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [10]
Lodoacetamide azide
 Probe Info  		C219(0.00); C49(0.00)  LDD0037  [9]
BTD
 Probe Info  		N.A.  LDD0004  [11]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [12]
NAIA_4
 Probe Info  		N.A.  LDD2226  [13]
TFBX
 Probe Info  		N.A.  LDD0027  [12]
WYneN
 Probe Info  		N.A.  LDD0021  [11]
IPM
 Probe Info  		N.A.  LDD0005  [11]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [14]
Acrolein
 Probe Info  		N.A.  LDD0217  [15]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [15]
NAIA_5
 Probe Info  		C219(0.00); C49(0.00)  LDD2223  [13]
PAL-AfBPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C187
 Probe Info  		31.34  LDD1865  [16]
C191
 Probe Info  		26.91  LDD1868  [16]
C193
 Probe Info  		11.55  LDD1869  [16]
FFF probe11
 Probe Info  		7.36  LDD0471  [17]
FFF probe13
 Probe Info  		10.79  LDD0475  [17]
FFF probe14
 Probe Info  		15.92  LDD0477  [17]
FFF probe2
 Probe Info  		7.51  LDD0463  [17]
VE-P
 Probe Info  		N.A.  LDD0396  [18]
DA-2
 Probe Info  		N.A.  LDD0072  [19]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C219(0.67)  LDD2142  [20]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C219(0.97)  LDD2112  [20]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C219(0.70)  LDD2095  [20]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C219(0.97)  LDD2117  [20]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C219(1.09)  LDD2152  [20]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C219(0.52)  LDD2132  [20]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C219(0.70)  LDD2131  [20]
 LDCM0025  4SU-RNA HEK-293T C219(4.32)  LDD0168  [5]
 LDCM0026  4SU-RNA+native RNA HEK-293T C219(3.25)  LDD0169  [5]
 LDCM0214  AC1 HEK-293T C219(0.85); C49(1.03)  LDD1507  [21]
 LDCM0215  AC10 HEK-293T C219(0.86)  LDD1508  [21]
 LDCM0226  AC11 HEK-293T C219(0.93)  LDD1509  [21]
 LDCM0237  AC12 HEK-293T C219(0.89)  LDD1510  [21]
 LDCM0259  AC14 HEK-293T C219(0.96); C49(1.35)  LDD1512  [21]
 LDCM0270  AC15 HEK-293T C219(0.92)  LDD1513  [21]
 LDCM0276  AC17 HEK-293T C219(0.87); C49(0.90)  LDD1515  [21]
 LDCM0277  AC18 HEK-293T C219(0.96)  LDD1516  [21]
 LDCM0278  AC19 HEK-293T C219(0.82)  LDD1517  [21]
 LDCM0279  AC2 HEK-293T C219(1.03)  LDD1518  [21]
 LDCM0280  AC20 HEK-293T C219(0.94)  LDD1519  [21]
 LDCM0281  AC21 HEK-293T C219(0.91)  LDD1520  [21]
 LDCM0282  AC22 HEK-293T C219(0.91); C49(1.26)  LDD1521  [21]
 LDCM0283  AC23 HEK-293T C219(1.07)  LDD1522  [21]
 LDCM0284  AC24 HEK-293T C219(1.06); C49(1.09)  LDD1523  [21]
 LDCM0285  AC25 HEK-293T C219(0.87); C49(1.02)  LDD1524  [21]
 LDCM0286  AC26 HEK-293T C219(0.92)  LDD1525  [21]
 LDCM0287  AC27 HEK-293T C219(0.91)  LDD1526  [21]
 LDCM0288  AC28 HEK-293T C219(0.90)  LDD1527  [21]
 LDCM0289  AC29 HEK-293T C219(0.96)  LDD1528  [21]
 LDCM0290  AC3 HEK-293T C219(0.95)  LDD1529  [21]
 LDCM0291  AC30 HEK-293T C219(0.91); C49(1.11)  LDD1530  [21]
 LDCM0292  AC31 HEK-293T C219(1.00)  LDD1531  [21]
 LDCM0293  AC32 HEK-293T C219(0.98); C49(1.23)  LDD1532  [21]
 LDCM0294  AC33 HEK-293T C219(0.78); C49(0.91)  LDD1533  [21]
 LDCM0295  AC34 HEK-293T C219(0.82)  LDD1534  [21]
 LDCM0296  AC35 HEK-293T C219(0.87)  LDD1535  [21]
 LDCM0297  AC36 HEK-293T C219(0.82)  LDD1536  [21]
 LDCM0298  AC37 HEK-293T C219(0.91)  LDD1537  [21]
 LDCM0299  AC38 HEK-293T C219(0.87); C49(1.59)  LDD1538  [21]
 LDCM0300  AC39 HEK-293T C219(0.88)  LDD1539  [21]
 LDCM0301  AC4 HEK-293T C219(0.92)  LDD1540  [21]
 LDCM0302  AC40 HEK-293T C219(0.82); C49(1.16)  LDD1541  [21]
 LDCM0303  AC41 HEK-293T C219(0.77); C49(0.98)  LDD1542  [21]
 LDCM0304  AC42 HEK-293T C219(0.80)  LDD1543  [21]
 LDCM0305  AC43 HEK-293T C219(0.87)  LDD1544  [21]
 LDCM0306  AC44 HEK-293T C219(0.84)  LDD1545  [21]
 LDCM0307  AC45 HEK-293T C219(0.81)  LDD1546  [21]
 LDCM0308  AC46 HEK-293T C219(0.90); C49(1.51)  LDD1547  [21]
 LDCM0309  AC47 HEK-293T C219(0.90)  LDD1548  [21]
 LDCM0310  AC48 HEK-293T C219(0.91); C49(1.10)  LDD1549  [21]
 LDCM0311  AC49 HEK-293T C219(0.85); C49(0.92)  LDD1550  [21]
 LDCM0312  AC5 HEK-293T C219(0.94)  LDD1551  [21]
 LDCM0313  AC50 HEK-293T C219(0.93)  LDD1552  [21]
 LDCM0314  AC51 HEK-293T C219(0.96)  LDD1553  [21]
 LDCM0315  AC52 HEK-293T C219(0.86)  LDD1554  [21]
 LDCM0316  AC53 HEK-293T C219(0.91)  LDD1555  [21]
 LDCM0317  AC54 HEK-293T C219(0.89); C49(1.29)  LDD1556  [21]
 LDCM0318  AC55 HEK-293T C219(0.93)  LDD1557  [21]
 LDCM0319  AC56 HEK-293T C219(0.93); C49(1.04)  LDD1558  [21]
 LDCM0320  AC57 HEK-293T C219(0.86); C49(0.86)  LDD1559  [21]
 LDCM0321  AC58 HEK-293T C219(0.86)  LDD1560  [21]
 LDCM0322  AC59 HEK-293T C219(0.88)  LDD1561  [21]
 LDCM0323  AC6 HEK-293T C219(0.93); C49(1.36)  LDD1562  [21]
 LDCM0324  AC60 HEK-293T C219(0.87)  LDD1563  [21]
 LDCM0325  AC61 HEK-293T C219(0.86)  LDD1564  [21]
 LDCM0326  AC62 HEK-293T C219(0.92); C49(1.14)  LDD1565  [21]
 LDCM0327  AC63 HEK-293T C219(0.93)  LDD1566  [21]
 LDCM0328  AC64 HEK-293T C219(0.93); C49(1.13)  LDD1567  [21]
 LDCM0334  AC7 HEK-293T C219(1.03)  LDD1568  [21]
 LDCM0345  AC8 HEK-293T C219(0.97); C49(1.14)  LDD1569  [21]
 LDCM0545  Acetamide MDA-MB-231 C219(0.38)  LDD2138  [20]
 LDCM0248  AKOS034007472 HEK-293T C219(0.94)  LDD1511  [21]
 LDCM0356  AKOS034007680 HEK-293T C219(0.86); C49(0.82)  LDD1570  [21]
 LDCM0275  AKOS034007705 HEK-293T C219(0.93); C49(1.05)  LDD1514  [21]
 LDCM0156  Aniline NCI-H1299 11.76  LDD0403  [3]
 LDCM0498  BS-3668 MDA-MB-231 C219(0.37)  LDD2091  [20]
 LDCM0630  CCW28-3 231MFP C49(1.58); C219(1.36)  LDD2214  [22]
 LDCM0632  CL-Sc Hep-G2 C219(1.20)  LDD2227  [13]
 LDCM0367  CL1 HEK-293T C219(0.93); C49(1.00)  LDD1571  [21]
 LDCM0368  CL10 HEK-293T C219(0.66); C49(1.83)  LDD1572  [21]
 LDCM0369  CL100 HEK-293T C219(0.97); C49(1.46)  LDD1573  [21]
 LDCM0370  CL101 HEK-293T C219(1.04); C49(0.96)  LDD1574  [21]
 LDCM0371  CL102 HEK-293T C219(0.80); C49(0.94)  LDD1575  [21]
 LDCM0372  CL103 HEK-293T C219(0.84); C49(0.94)  LDD1576  [21]
 LDCM0373  CL104 HEK-293T C219(1.03); C49(0.99)  LDD1577  [21]
 LDCM0374  CL105 HEK-293T C219(1.00); C49(1.29)  LDD1578  [21]
 LDCM0375  CL106 HEK-293T C219(0.91); C49(0.88)  LDD1579  [21]
 LDCM0376  CL107 HEK-293T C219(0.90); C49(1.10)  LDD1580  [21]
 LDCM0377  CL108 HEK-293T C219(0.93); C49(1.02)  LDD1581  [21]
 LDCM0378  CL109 HEK-293T C219(0.99); C49(1.02)  LDD1582  [21]
 LDCM0379  CL11 HEK-293T C219(0.85)  LDD1583  [21]
 LDCM0380  CL110 HEK-293T C219(0.82); C49(0.95)  LDD1584  [21]
 LDCM0381  CL111 HEK-293T C219(0.86); C49(0.96)  LDD1585  [21]
 LDCM0382  CL112 HEK-293T C219(0.86); C49(1.26)  LDD1586  [21]
 LDCM0383  CL113 HEK-293T C219(0.98); C49(0.87)  LDD1587  [21]
 LDCM0384  CL114 HEK-293T C219(0.75); C49(0.80)  LDD1588  [21]
 LDCM0385  CL115 HEK-293T C219(0.75); C49(0.96)  LDD1589  [21]
 LDCM0386  CL116 HEK-293T C219(0.98); C49(1.06)  LDD1590  [21]
 LDCM0387  CL117 HEK-293T C219(0.97); C49(1.01)  LDD1591  [21]
 LDCM0388  CL118 HEK-293T C219(0.83); C49(0.96)  LDD1592  [21]
 LDCM0389  CL119 HEK-293T C219(0.81); C49(0.92)  LDD1593  [21]
 LDCM0390  CL12 HEK-293T C219(0.90); C49(1.28)  LDD1594  [21]
 LDCM0391  CL120 HEK-293T C219(1.10); C49(1.54)  LDD1595  [21]
 LDCM0392  CL121 HEK-293T C219(1.00); C49(0.97)  LDD1596  [21]
 LDCM0393  CL122 HEK-293T C219(0.89); C49(0.98)  LDD1597  [21]
 LDCM0394  CL123 HEK-293T C219(0.77); C49(1.04)  LDD1598  [21]
 LDCM0395  CL124 HEK-293T C219(1.00); C49(0.83)  LDD1599  [21]
 LDCM0396  CL125 HEK-293T C219(0.91); C49(1.15)  LDD1600  [21]
 LDCM0397  CL126 HEK-293T C219(0.82); C49(0.88)  LDD1601  [21]
 LDCM0398  CL127 HEK-293T C219(0.89); C49(0.99)  LDD1602  [21]
 LDCM0399  CL128 HEK-293T C219(1.03); C49(0.96)  LDD1603  [21]
 LDCM0400  CL13 HEK-293T C219(0.90); C49(1.01)  LDD1604  [21]
 LDCM0401  CL14 HEK-293T C219(0.90); C49(0.96)  LDD1605  [21]
 LDCM0402  CL15 HEK-293T C219(0.70); C49(0.74)  LDD1606  [21]
 LDCM0403  CL16 HEK-293T C219(1.09); C49(0.82)  LDD1607  [21]
 LDCM0404  CL17 HEK-293T C219(0.65); C49(0.98)  LDD1608  [21]
 LDCM0405  CL18 HEK-293T C219(0.87)  LDD1609  [21]
 LDCM0406  CL19 HEK-293T C219(0.90)  LDD1610  [21]
 LDCM0407  CL2 HEK-293T C219(0.93); C49(0.97)  LDD1611  [21]
 LDCM0408  CL20 HEK-293T C219(0.80)  LDD1612  [21]
 LDCM0409  CL21 HEK-293T C219(0.75)  LDD1613  [21]
 LDCM0410  CL22 HEK-293T C219(0.88); C49(1.53)  LDD1614  [21]
 LDCM0411  CL23 HEK-293T C219(0.87)  LDD1615  [21]
 LDCM0412  CL24 HEK-293T C219(0.90); C49(1.28)  LDD1616  [21]
 LDCM0413  CL25 HEK-293T C219(0.94); C49(1.06)  LDD1617  [21]
 LDCM0414  CL26 HEK-293T C219(0.97); C49(0.93)  LDD1618  [21]
 LDCM0415  CL27 HEK-293T C219(0.96); C49(0.99)  LDD1619  [21]
 LDCM0416  CL28 HEK-293T C219(1.00); C49(0.98)  LDD1620  [21]
 LDCM0417  CL29 HEK-293T C219(0.86); C49(1.03)  LDD1621  [21]
 LDCM0418  CL3 HEK-293T C219(0.88); C49(0.89)  LDD1622  [21]
 LDCM0419  CL30 HEK-293T C219(1.04)  LDD1623  [21]
 LDCM0420  CL31 HEK-293T C219(0.89)  LDD1624  [21]
 LDCM0421  CL32 HEK-293T C219(0.95)  LDD1625  [21]
 LDCM0422  CL33 HEK-293T C219(0.78)  LDD1626  [21]
 LDCM0423  CL34 HEK-293T C219(0.89); C49(1.26)  LDD1627  [21]
 LDCM0424  CL35 HEK-293T C219(0.92)  LDD1628  [21]
 LDCM0425  CL36 HEK-293T C219(1.00); C49(1.14)  LDD1629  [21]
 LDCM0426  CL37 HEK-293T C219(1.01); C49(1.08)  LDD1630  [21]
 LDCM0428  CL39 HEK-293T C219(0.86); C49(0.99)  LDD1632  [21]
 LDCM0429  CL4 HEK-293T C219(1.03); C49(0.69)  LDD1633  [21]
 LDCM0430  CL40 HEK-293T C219(1.02); C49(1.03)  LDD1634  [21]
 LDCM0431  CL41 HEK-293T C219(0.81); C49(0.93)  LDD1635  [21]
 LDCM0432  CL42 HEK-293T C219(1.00)  LDD1636  [21]
 LDCM0433  CL43 HEK-293T C219(1.03)  LDD1637  [21]
 LDCM0434  CL44 HEK-293T C219(0.92)  LDD1638  [21]
 LDCM0435  CL45 HEK-293T C219(0.85)  LDD1639  [21]
 LDCM0436  CL46 HEK-293T C219(0.93); C49(1.57)  LDD1640  [21]
 LDCM0437  CL47 HEK-293T C219(0.94)  LDD1641  [21]
 LDCM0438  CL48 HEK-293T C219(1.04); C49(1.64)  LDD1642  [21]
 LDCM0439  CL49 HEK-293T C219(1.00); C49(0.97)  LDD1643  [21]
 LDCM0440  CL5 HEK-293T C219(0.77); C49(1.01)  LDD1644  [21]
 LDCM0441  CL50 HEK-293T C219(0.81); C49(0.92)  LDD1645  [21]
 LDCM0443  CL52 HEK-293T C219(1.15); C49(1.07)  LDD1646  [21]
 LDCM0444  CL53 HEK-293T C219(0.73); C49(0.93)  LDD1647  [21]
 LDCM0445  CL54 HEK-293T C219(0.70)  LDD1648  [21]
 LDCM0446  CL55 HEK-293T C219(0.89)  LDD1649  [21]
 LDCM0447  CL56 HEK-293T C219(0.81)  LDD1650  [21]
 LDCM0448  CL57 HEK-293T C219(0.73)  LDD1651  [21]
 LDCM0449  CL58 HEK-293T C219(0.79); C49(1.53)  LDD1652  [21]
 LDCM0450  CL59 HEK-293T C219(0.83)  LDD1653  [21]
 LDCM0451  CL6 HEK-293T C219(0.79)  LDD1654  [21]
 LDCM0452  CL60 HEK-293T C219(0.89); C49(1.37)  LDD1655  [21]
 LDCM0453  CL61 HEK-293T C219(1.01); C49(0.91)  LDD1656  [21]
 LDCM0454  CL62 HEK-293T C219(0.91); C49(0.87)  LDD1657  [21]
 LDCM0455  CL63 HEK-293T C219(0.92); C49(0.95)  LDD1658  [21]
 LDCM0456  CL64 HEK-293T C219(0.99); C49(0.82)  LDD1659  [21]
 LDCM0457  CL65 HEK-293T C219(0.85); C49(1.06)  LDD1660  [21]
 LDCM0458  CL66 HEK-293T C219(0.87)  LDD1661  [21]
 LDCM0459  CL67 HEK-293T C219(0.96)  LDD1662  [21]
 LDCM0460  CL68 HEK-293T C219(0.82)  LDD1663  [21]
 LDCM0461  CL69 HEK-293T C219(0.85)  LDD1664  [21]
 LDCM0462  CL7 HEK-293T C219(0.90)  LDD1665  [21]
 LDCM0463  CL70 HEK-293T C219(0.88); C49(1.85)  LDD1666  [21]
 LDCM0464  CL71 HEK-293T C219(0.89)  LDD1667  [21]
 LDCM0465  CL72 HEK-293T C219(0.91); C49(1.17)  LDD1668  [21]
 LDCM0466  CL73 HEK-293T C219(1.05); C49(1.05)  LDD1669  [21]
 LDCM0467  CL74 HEK-293T C219(0.88); C49(0.89)  LDD1670  [21]
 LDCM0469  CL76 HEK-293T C219(1.01); C49(0.87)  LDD1672  [21]
 LDCM0470  CL77 HEK-293T C219(0.75); C49(1.01)  LDD1673  [21]
 LDCM0471  CL78 HEK-293T C219(0.90)  LDD1674  [21]
 LDCM0472  CL79 HEK-293T C219(0.93)  LDD1675  [21]
 LDCM0473  CL8 HEK-293T C219(0.57)  LDD1676  [21]
 LDCM0474  CL80 HEK-293T C219(0.93)  LDD1677  [21]
 LDCM0475  CL81 HEK-293T C219(0.91)  LDD1678  [21]
 LDCM0476  CL82 HEK-293T C219(0.92); C49(1.21)  LDD1679  [21]
 LDCM0477  CL83 HEK-293T C219(0.90)  LDD1680  [21]
 LDCM0478  CL84 HEK-293T C219(0.88); C49(1.41)  LDD1681  [21]
 LDCM0479  CL85 HEK-293T C219(0.99); C49(1.13)  LDD1682  [21]
 LDCM0480  CL86 HEK-293T C219(0.89); C49(1.03)  LDD1683  [21]
 LDCM0481  CL87 HEK-293T C219(0.79); C49(0.93)  LDD1684  [21]
 LDCM0482  CL88 HEK-293T C219(1.18); C49(1.12)  LDD1685  [21]
 LDCM0483  CL89 HEK-293T C219(0.82); C49(0.84)  LDD1686  [21]
 LDCM0484  CL9 HEK-293T C219(0.90)  LDD1687  [21]
 LDCM0485  CL90 HEK-293T C219(0.62)  LDD1688  [21]
 LDCM0486  CL91 HEK-293T C219(0.92)  LDD1689  [21]
 LDCM0487  CL92 HEK-293T C219(0.74)  LDD1690  [21]
 LDCM0488  CL93 HEK-293T C219(0.82)  LDD1691  [21]
 LDCM0489  CL94 HEK-293T C219(0.87); C49(1.76)  LDD1692  [21]
 LDCM0490  CL95 HEK-293T C219(0.72)  LDD1693  [21]
 LDCM0491  CL96 HEK-293T C219(0.81); C49(1.08)  LDD1694  [21]
 LDCM0492  CL97 HEK-293T C219(0.97); C49(1.11)  LDD1695  [21]
 LDCM0493  CL98 HEK-293T C219(0.85); C49(1.06)  LDD1696  [21]
 LDCM0494  CL99 HEK-293T C219(0.86); C49(1.00)  LDD1697  [21]
 LDCM0495  E2913 HEK-293T C219(0.85); C49(0.90)  LDD1698  [21]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C219(0.80)  LDD1702  [20]
 LDCM0625  F8 Ramos C219(1.11); C49(1.29)  LDD2187  [23]
 LDCM0572  Fragment10 Ramos C219(0.60)  LDD2189  [23]
 LDCM0573  Fragment11 Ramos C219(0.00); C49(0.17)  LDD2190  [23]
 LDCM0574  Fragment12 Ramos C219(0.60)  LDD2191  [23]
 LDCM0575  Fragment13 Ramos C219(0.92)  LDD2192  [23]
 LDCM0576  Fragment14 Ramos C219(0.65); C49(0.82)  LDD2193  [23]
 LDCM0579  Fragment20 Ramos C219(0.49)  LDD2194  [23]
 LDCM0580  Fragment21 Ramos C219(0.86)  LDD2195  [23]
 LDCM0582  Fragment23 Ramos C219(0.96)  LDD2196  [23]
 LDCM0578  Fragment27 Ramos C219(0.91)  LDD2197  [23]
 LDCM0586  Fragment28 Ramos C219(0.96); C49(0.27)  LDD2198  [23]
 LDCM0588  Fragment30 Ramos C219(1.04)  LDD2199  [23]
 LDCM0589  Fragment31 Ramos C219(0.82)  LDD2200  [23]
 LDCM0590  Fragment32 Ramos C219(0.68)  LDD2201  [23]
 LDCM0468  Fragment33 HEK-293T C219(0.85); C49(0.95)  LDD1671  [21]
 LDCM0596  Fragment38 Ramos C219(1.14)  LDD2203  [23]
 LDCM0566  Fragment4 Ramos C219(0.72); C49(0.60)  LDD2184  [23]
 LDCM0427  Fragment51 HEK-293T C219(0.93); C49(0.86)  LDD1631  [21]
 LDCM0610  Fragment52 Ramos C219(2.55)  LDD2204  [23]
 LDCM0614  Fragment56 Ramos C219(1.18)  LDD2205  [23]
 LDCM0569  Fragment7 Ramos C219(0.48); C49(0.94)  LDD2186  [23]
 LDCM0571  Fragment9 Ramos C219(0.58)  LDD2188  [23]
 LDCM0022  KB02 HEK-293T C219(0.95); C49(0.92)  LDD1492  [21]
 LDCM0023  KB03 HEK-293T C219(1.03); C49(0.98)  LDD1497  [21]
 LDCM0024  KB05 G361 C307(1.10)  LDD3311  [4]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C219(1.03)  LDD2102  [20]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C219(0.41)  LDD2121  [20]
 LDCM0109  NEM HeLa N.A.  LDD0224  [15]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C219(0.51)  LDD2089  [20]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C219(1.48)  LDD2090  [20]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C219(0.81)  LDD2092  [20]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C219(0.90)  LDD2093  [20]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C219(1.07)  LDD2094  [20]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C219(0.47)  LDD2096  [20]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C219(0.87)  LDD2097  [20]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C219(0.67)  LDD2098  [20]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C219(0.63)  LDD2099  [20]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C219(0.69)  LDD2100  [20]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C219(0.76)  LDD2104  [20]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C219(1.56)  LDD2105  [20]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C219(0.59)  LDD2106  [20]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C219(0.86)  LDD2107  [20]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C219(0.75)  LDD2108  [20]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C219(0.68)  LDD2109  [20]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C219(0.91)  LDD2110  [20]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C219(0.79)  LDD2111  [20]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C219(0.62)  LDD2114  [20]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C219(1.02)  LDD2115  [20]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C219(0.85)  LDD2116  [20]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C219(0.78)  LDD2118  [20]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C219(2.30)  LDD2119  [20]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C219(0.98)  LDD2120  [20]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C219(1.06)  LDD2122  [20]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C219(0.66)  LDD2123  [20]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C219(0.94)  LDD2124  [20]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C219(0.72)  LDD2125  [20]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C219(0.89)  LDD2126  [20]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C219(0.71)  LDD2127  [20]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C219(0.62)  LDD2129  [20]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C219(0.55)  LDD2133  [20]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C219(0.75)  LDD2134  [20]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C219(0.76)  LDD2135  [20]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C219(0.77)  LDD2136  [20]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C219(0.83)  LDD2137  [20]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C219(0.97)  LDD1700  [20]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C219(0.68)  LDD2140  [20]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C219(0.51)  LDD2141  [20]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C219(1.03)  LDD2143  [20]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C219(0.88)  LDD2146  [20]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C219(3.12)  LDD2147  [20]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C219(0.66)  LDD2148  [20]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C219(0.96)  LDD2149  [20]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C219(0.33)  LDD2150  [20]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C219(0.91)  LDD2151  [20]
 LDCM0627  NUDT7-COV-1 HEK-293T C219(0.88)  LDD2206  [24]
 LDCM0628  OTUB2-COV-1 HEK-293T C219(1.37); C219(1.00)  LDD2207  [24]
 LDCM0131  RA190 MM1.R C219(1.13)  LDD0304  [25]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Glyceraldehyde-3-phosphate dehydrogenase family P04406
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (SMARCAL1) SNF2/RAD54 helicase family Q9NZC9
Uracil-DNA glycosylase (UNG) UNG family P13051
E3 ubiquitin-protein ligase RFWD3 (RFWD3) . Q6PCD5
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cell division control protein 45 homolog (CDC45) CDC45 family O75419
TIMELESS-interacting protein (TIPIN) CSM3 family Q9BVW5
Cyclin-C (CCNC) Cyclin family P24863
Eukaryotic translation initiation factor 4E-binding protein 3 (EIF4EBP3) EIF4E-binding protein family O60516
Replication protein A 70 kDa DNA-binding subunit (RPA1) Replication factor A protein 1 family P27694
Replication protein A 14 kDa subunit (RPA3) Replication factor A protein 3 family P35244
DNA repair protein complementing XP-A cells (XPA) XPA family P23025
SERTA domain-containing protein 3 (SERTAD3) . Q9UJW9

References

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Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
6 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
13 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
14 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
15 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
16 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
17 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
18 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
19 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
20 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
21 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
22 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
23 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
24 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
25 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.