Details of the Target

General Information of Target

Target ID LDTP07989
Target Name Ribonucleoside-diphosphate reductase subunit M2 B (RRM2B)
Gene Name RRM2B
Gene ID 50484
Synonyms
P53R2; Ribonucleoside-diphosphate reductase subunit M2 B; EC 1.17.4.1; TP53-inducible ribonucleotide reductase M2 B; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQ
ASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEA
RCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRK
STFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQ
YLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFS
KVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF
Target Bioclass
Enzyme
Family
Ribonucleoside diphosphate reductase small chain family
Subcellular location
Cytoplasm
Function
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
Uniprot ID
Q7LG56
Ensemble ID
ENST00000251810.8
HGNC ID
HGNC:17296
ChEMBL ID
CHEMBL3301398

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT116 SNV: p.V115L .
HT Deletion: p.K37RfsTer57 .
NCIH2286 SNV: p.L140S .
REH SNV: p.M214I DBIA    Probe Info 
SNU1 SNV: p.A290T DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		7.86  LDD0402  [1]
Alkylaryl probe 2
 Probe Info  		7.00  LDD0391  [2]
Alkylaryl probe 3
 Probe Info  		20.00  LDD0381  [2]
DBIA
 Probe Info  		C67(1.77)  LDD3312  [3]
AHL-Pu-1
 Probe Info  		C279(10.60)  LDD0168  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
NAIA_5
 Probe Info  		N.A.  LDD2223  [6]
PAL-AfBPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C106
 Probe Info  		17.75  LDD1793  [7]
C108
 Probe Info  		12.82  LDD1795  [7]
C186
 Probe Info  		8.82  LDD1864  [7]
C264
 Probe Info  		23.92  LDD1935  [7]
C284
 Probe Info  		24.76  LDD1954  [7]
C343
 Probe Info  		13.45  LDD2005  [7]
C380
 Probe Info  		5.13  LDD2039  [7]
C382
 Probe Info  		12.55  LDD2041  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C279(10.60)  LDD0168  [4]
 LDCM0026  4SU-RNA+native RNA DM93 C279(2.92)  LDD0171  [4]
 LDCM0214  AC1 HEK-293T C279(0.96)  LDD1507  [8]
 LDCM0215  AC10 HEK-293T C279(1.30); C232(0.90)  LDD1508  [8]
 LDCM0237  AC12 HEK-293T C232(0.92)  LDD1510  [8]
 LDCM0276  AC17 HEK-293T C279(0.71)  LDD1515  [8]
 LDCM0277  AC18 HEK-293T C279(1.22); C232(0.91)  LDD1516  [8]
 LDCM0279  AC2 HEK-293T C279(0.88); C232(0.94)  LDD1518  [8]
 LDCM0280  AC20 HEK-293T C232(0.88)  LDD1519  [8]
 LDCM0281  AC21 HEK-293T C279(0.94)  LDD1520  [8]
 LDCM0284  AC24 HEK-293T C279(1.05); C232(0.95)  LDD1523  [8]
 LDCM0285  AC25 HEK-293T C279(0.90)  LDD1524  [8]
 LDCM0286  AC26 HEK-293T C279(1.07); C232(0.89)  LDD1525  [8]
 LDCM0288  AC28 HEK-293T C232(0.98)  LDD1527  [8]
 LDCM0289  AC29 HEK-293T C279(0.94)  LDD1528  [8]
 LDCM0293  AC32 HEK-293T C279(1.24); C232(0.95)  LDD1532  [8]
 LDCM0294  AC33 HEK-293T C279(0.83)  LDD1533  [8]
 LDCM0295  AC34 HEK-293T C279(1.03); C232(0.89)  LDD1534  [8]
 LDCM0297  AC36 HEK-293T C232(0.99)  LDD1536  [8]
 LDCM0298  AC37 HEK-293T C279(1.06)  LDD1537  [8]
 LDCM0301  AC4 HEK-293T C232(0.99)  LDD1540  [8]
 LDCM0302  AC40 HEK-293T C279(1.23); C232(1.04)  LDD1541  [8]
 LDCM0303  AC41 HEK-293T C279(0.96)  LDD1542  [8]
 LDCM0304  AC42 HEK-293T C279(0.74); C232(0.87)  LDD1543  [8]
 LDCM0306  AC44 HEK-293T C232(0.99)  LDD1545  [8]
 LDCM0307  AC45 HEK-293T C279(1.08)  LDD1546  [8]
 LDCM0310  AC48 HEK-293T C279(1.08); C232(0.84)  LDD1549  [8]
 LDCM0311  AC49 HEK-293T C279(0.88)  LDD1550  [8]
 LDCM0312  AC5 HEK-293T C279(1.07)  LDD1551  [8]
 LDCM0313  AC50 HEK-293T C279(0.95); C232(0.83)  LDD1552  [8]
 LDCM0315  AC52 HEK-293T C232(0.85)  LDD1554  [8]
 LDCM0316  AC53 HEK-293T C279(0.98)  LDD1555  [8]
 LDCM0319  AC56 HEK-293T C279(0.99); C232(0.92)  LDD1558  [8]
 LDCM0320  AC57 HEK-293T C279(0.95)  LDD1559  [8]
 LDCM0321  AC58 HEK-293T C279(1.15); C232(0.92)  LDD1560  [8]
 LDCM0324  AC60 HEK-293T C232(0.86)  LDD1563  [8]
 LDCM0325  AC61 HEK-293T C279(1.03)  LDD1564  [8]
 LDCM0328  AC64 HEK-293T C279(1.11); C232(0.87)  LDD1567  [8]
 LDCM0345  AC8 HEK-293T C279(1.01); C232(0.99)  LDD1569  [8]
 LDCM0248  AKOS034007472 HEK-293T C279(0.90)  LDD1511  [8]
 LDCM0356  AKOS034007680 HEK-293T C279(0.91)  LDD1570  [8]
 LDCM0275  AKOS034007705 HEK-293T C279(1.07); C232(0.89)  LDD1514  [8]
 LDCM0156  Aniline NCI-H1299 8.79  LDD0403  [1]
 LDCM0369  CL100 HEK-293T C279(0.84)  LDD1573  [8]
 LDCM0371  CL102 HEK-293T C279(0.85)  LDD1575  [8]
 LDCM0372  CL103 HEK-293T C279(0.84); C232(0.81)  LDD1576  [8]
 LDCM0373  CL104 HEK-293T C279(0.90)  LDD1577  [8]
 LDCM0375  CL106 HEK-293T C279(0.75)  LDD1579  [8]
 LDCM0376  CL107 HEK-293T C279(0.83); C232(0.98)  LDD1580  [8]
 LDCM0377  CL108 HEK-293T C279(0.77)  LDD1581  [8]
 LDCM0380  CL110 HEK-293T C279(0.96)  LDD1584  [8]
 LDCM0381  CL111 HEK-293T C279(0.90); C232(0.86)  LDD1585  [8]
 LDCM0382  CL112 HEK-293T C279(0.97)  LDD1586  [8]
 LDCM0384  CL114 HEK-293T C279(0.94)  LDD1588  [8]
 LDCM0385  CL115 HEK-293T C279(1.40); C232(0.84)  LDD1589  [8]
 LDCM0386  CL116 HEK-293T C279(0.69)  LDD1590  [8]
 LDCM0388  CL118 HEK-293T C279(0.98)  LDD1592  [8]
 LDCM0389  CL119 HEK-293T C279(0.88); C232(0.90)  LDD1593  [8]
 LDCM0390  CL12 HEK-293T C279(1.10); C232(1.09)  LDD1594  [8]
 LDCM0391  CL120 HEK-293T C279(1.07)  LDD1595  [8]
 LDCM0393  CL122 HEK-293T C279(1.05)  LDD1597  [8]
 LDCM0394  CL123 HEK-293T C279(0.82); C232(0.91)  LDD1598  [8]
 LDCM0395  CL124 HEK-293T C279(0.97)  LDD1599  [8]
 LDCM0397  CL126 HEK-293T C279(0.97)  LDD1601  [8]
 LDCM0398  CL127 HEK-293T C279(1.08); C232(0.91)  LDD1602  [8]
 LDCM0399  CL128 HEK-293T C279(0.80)  LDD1603  [8]
 LDCM0401  CL14 HEK-293T C279(1.24)  LDD1605  [8]
 LDCM0402  CL15 HEK-293T C279(1.04); C232(0.98)  LDD1606  [8]
 LDCM0403  CL16 HEK-293T C279(0.72)  LDD1607  [8]
 LDCM0404  CL17 HEK-293T C279(0.84)  LDD1608  [8]
 LDCM0405  CL18 HEK-293T C279(1.00); C232(0.91)  LDD1609  [8]
 LDCM0407  CL2 HEK-293T C279(1.11)  LDD1611  [8]
 LDCM0408  CL20 HEK-293T C232(1.07)  LDD1612  [8]
 LDCM0409  CL21 HEK-293T C279(0.82)  LDD1613  [8]
 LDCM0412  CL24 HEK-293T C279(1.09); C232(1.30)  LDD1616  [8]
 LDCM0414  CL26 HEK-293T C279(0.96)  LDD1618  [8]
 LDCM0415  CL27 HEK-293T C279(0.89); C232(1.06)  LDD1619  [8]
 LDCM0416  CL28 HEK-293T C279(0.88)  LDD1620  [8]
 LDCM0417  CL29 HEK-293T C279(0.86)  LDD1621  [8]
 LDCM0418  CL3 HEK-293T C279(1.01); C232(0.85)  LDD1622  [8]
 LDCM0419  CL30 HEK-293T C279(1.16); C232(0.77)  LDD1623  [8]
 LDCM0421  CL32 HEK-293T C232(0.96)  LDD1625  [8]
 LDCM0422  CL33 HEK-293T C279(0.86)  LDD1626  [8]
 LDCM0425  CL36 HEK-293T C279(1.14); C232(1.06)  LDD1629  [8]
 LDCM0428  CL39 HEK-293T C279(1.03); C232(1.23)  LDD1632  [8]
 LDCM0429  CL4 HEK-293T C279(0.72)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C279(0.92)  LDD1634  [8]
 LDCM0431  CL41 HEK-293T C279(0.89)  LDD1635  [8]
 LDCM0432  CL42 HEK-293T C279(0.94); C232(0.90)  LDD1636  [8]
 LDCM0434  CL44 HEK-293T C232(0.99)  LDD1638  [8]
 LDCM0435  CL45 HEK-293T C279(0.90)  LDD1639  [8]
 LDCM0438  CL48 HEK-293T C279(0.89); C232(1.04)  LDD1642  [8]
 LDCM0440  CL5 HEK-293T C279(1.04)  LDD1644  [8]
 LDCM0441  CL50 HEK-293T C279(0.83)  LDD1645  [8]
 LDCM0443  CL52 HEK-293T C279(0.75)  LDD1646  [8]
 LDCM0444  CL53 HEK-293T C279(0.80)  LDD1647  [8]
 LDCM0445  CL54 HEK-293T C279(0.79); C232(0.85)  LDD1648  [8]
 LDCM0447  CL56 HEK-293T C232(0.93)  LDD1650  [8]
 LDCM0448  CL57 HEK-293T C279(1.01)  LDD1651  [8]
 LDCM0451  CL6 HEK-293T C279(0.98); C232(0.83)  LDD1654  [8]
 LDCM0452  CL60 HEK-293T C279(0.99); C232(1.14)  LDD1655  [8]
 LDCM0454  CL62 HEK-293T C279(0.95)  LDD1657  [8]
 LDCM0455  CL63 HEK-293T C279(0.99); C232(0.83)  LDD1658  [8]
 LDCM0456  CL64 HEK-293T C279(0.73)  LDD1659  [8]
 LDCM0457  CL65 HEK-293T C279(0.87)  LDD1660  [8]
 LDCM0458  CL66 HEK-293T C279(0.97); C232(0.85)  LDD1661  [8]
 LDCM0460  CL68 HEK-293T C232(1.19)  LDD1663  [8]
 LDCM0461  CL69 HEK-293T C279(1.05)  LDD1664  [8]
 LDCM0465  CL72 HEK-293T C279(1.05); C232(1.09)  LDD1668  [8]
 LDCM0467  CL74 HEK-293T C279(0.97)  LDD1670  [8]
 LDCM0469  CL76 HEK-293T C279(0.74)  LDD1672  [8]
 LDCM0470  CL77 HEK-293T C279(0.90)  LDD1673  [8]
 LDCM0471  CL78 HEK-293T C279(1.14); C232(1.05)  LDD1674  [8]
 LDCM0473  CL8 HEK-293T C232(0.88)  LDD1676  [8]
 LDCM0474  CL80 HEK-293T C232(0.95)  LDD1677  [8]
 LDCM0475  CL81 HEK-293T C279(1.06)  LDD1678  [8]
 LDCM0478  CL84 HEK-293T C279(0.87); C232(1.22)  LDD1681  [8]
 LDCM0480  CL86 HEK-293T C279(1.03)  LDD1683  [8]
 LDCM0481  CL87 HEK-293T C279(0.93); C232(1.05)  LDD1684  [8]
 LDCM0482  CL88 HEK-293T C279(0.83)  LDD1685  [8]
 LDCM0483  CL89 HEK-293T C279(0.97)  LDD1686  [8]
 LDCM0484  CL9 HEK-293T C279(1.01)  LDD1687  [8]
 LDCM0485  CL90 HEK-293T C279(0.91); C232(0.80)  LDD1688  [8]
 LDCM0487  CL92 HEK-293T C232(0.91)  LDD1690  [8]
 LDCM0488  CL93 HEK-293T C279(1.04)  LDD1691  [8]
 LDCM0491  CL96 HEK-293T C279(1.11); C232(1.04)  LDD1694  [8]
 LDCM0493  CL98 HEK-293T C279(1.10)  LDD1696  [8]
 LDCM0494  CL99 HEK-293T C279(1.75); C232(0.84)  LDD1697  [8]
 LDCM0495  E2913 HEK-293T C279(1.12); C232(0.95)  LDD1698  [8]
 LDCM0625  F8 Ramos C279(1.15)  LDD2187  [9]
 LDCM0572  Fragment10 Ramos C279(0.22)  LDD2189  [9]
 LDCM0573  Fragment11 Ramos C279(1.32)  LDD2190  [9]
 LDCM0574  Fragment12 Ramos C279(0.31)  LDD2191  [9]
 LDCM0575  Fragment13 Ramos C279(0.37)  LDD2192  [9]
 LDCM0576  Fragment14 Ramos C279(1.00)  LDD2193  [9]
 LDCM0579  Fragment20 Ramos C279(0.31)  LDD2194  [9]
 LDCM0580  Fragment21 Ramos C279(0.43)  LDD2195  [9]
 LDCM0582  Fragment23 Ramos C279(0.37)  LDD2196  [9]
 LDCM0578  Fragment27 Ramos C279(0.32)  LDD2197  [9]
 LDCM0586  Fragment28 Ramos C279(0.68)  LDD2198  [9]
 LDCM0588  Fragment30 Ramos C279(0.45)  LDD2199  [9]
 LDCM0589  Fragment31 Ramos C279(0.35)  LDD2200  [9]
 LDCM0590  Fragment32 Ramos C279(0.46)  LDD2201  [9]
 LDCM0468  Fragment33 HEK-293T C279(0.84); C232(0.94)  LDD1671  [8]
 LDCM0596  Fragment38 Ramos C279(0.16)  LDD2203  [9]
 LDCM0566  Fragment4 Ramos C279(1.04)  LDD2184  [9]
 LDCM0427  Fragment51 HEK-293T C279(0.82)  LDD1631  [8]
 LDCM0610  Fragment52 Ramos C279(0.34)  LDD2204  [9]
 LDCM0614  Fragment56 Ramos C279(0.33)  LDD2205  [9]
 LDCM0569  Fragment7 Ramos C279(1.71)  LDD2186  [9]
 LDCM0571  Fragment9 Ramos C279(0.45)  LDD2188  [9]
 LDCM0022  KB02 HEK-293T C279(1.07)  LDD1492  [8]
 LDCM0023  KB03 HEK-293T C279(0.97)  LDD1497  [8]
 LDCM0024  KB05 HMCB C67(1.77)  LDD3312  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase Mdm2 (MDM2) MDM2/MDM4 family Q00987
Serine-protein kinase ATM (ATM) PI3/PI4-kinase family Q13315
Ribonucleoside-diphosphate reductase subunit M2 B (RRM2B) Ribonucleoside diphosphate reductase small chain family Q7LG56
E3 ubiquitin-protein ligase NRDP1 (RNF41) . Q9H4P4
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Origin recognition complex subunit 4 (ORC4) ORC4 family O43929

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Cladribine Small molecular drug DB00242

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
9 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578