Details of the Target

General Information of Target

Target ID LDTP05274
Target Name Nucleolysin TIAR (TIAL1)
Gene Name TIAL1
Gene ID 7073
Synonyms
Nucleolysin TIAR; TIA-1-related protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEHTSNDPYCFVEFYEHRD
AAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPF
GKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAP
KSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEK
GYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVY
GNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIP
PPNQAGYGMASYQTQ
Target Bioclass
Other
Subcellular location
Nucleus
Function
RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation. Shows a preference for uridine-rich RNAs. Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA. Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1. Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Uniprot ID
Q01085
Ensemble ID
ENST00000369093.6
HGNC ID
HGNC:11804

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.S37G .
HT115 SNV: p.F117C .
JURKAT SNV: p.A154V .
MCC13 Substitution: p.P350L .
MFE296 Deletion: p.K240RfsTer21 .
MFE319 SNV: p.V333I .
NCIH1993 SNV: p.G344E .
TE11 SNV: p.G330R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ILS-1
 Probe Info  		2.18  LDD0415  [1]
TH211
 Probe Info  		Y242(20.00)  LDD0257  [2]
YN-1
 Probe Info  		100.00  LDD0444  [3]
DBIA
 Probe Info  		C35(1.25)  LDD3377  [4]
HHS-482
 Probe Info  		Y140(1.22)  LDD0285  [5]
HHS-475
 Probe Info  		Y140(0.91)  LDD0264  [6]
HHS-465
 Probe Info  		Y140(10.00); Y242(2.98)  LDD2237  [7]
Acrolein
 Probe Info  		H157(0.00); C209(0.00)  LDD0221  [8]
5E-2FA
 Probe Info  		H255(0.00); H157(0.00)  LDD2235  [9]
ATP probe
 Probe Info  		K79(0.00); K138(0.00)  LDD0199  [10]
m-APA
 Probe Info  		H255(0.00); H157(0.00)  LDD2231  [9]
4-Iodoacetamidophenylacetylene
 Probe Info  		C51(0.00); C35(0.00)  LDD0038  [11]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [12]
Lodoacetamide azide
 Probe Info  		C35(0.00); C51(0.00)  LDD0037  [11]
NHS
 Probe Info  		K79(0.00); K122(0.00)  LDD0010  [13]
SF
 Probe Info  		Y12(0.00); Y146(0.00); K176(0.00); Y140(0.00)  LDD0028  [14]
STPyne
 Probe Info  		N.A.  LDD0009  [13]
1c-yne
 Probe Info  		N.A.  LDD0228  [15]
1d-yne
 Probe Info  		K114(0.00); K148(0.00)  LDD0357  [15]
NAIA_5
 Probe Info  		C35(0.00); C51(0.00)  LDD2223  [16]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C165
 Probe Info  		13.64  LDD1845  [17]
C191
 Probe Info  		10.85  LDD1868  [17]
C232
 Probe Info  		35.02  LDD1905  [17]
FFF probe11
 Probe Info  		9.15  LDD0471  [18]
FFF probe13
 Probe Info  		20.00  LDD0475  [18]
FFF probe2
 Probe Info  		14.60  LDD0463  [18]
FFF probe4
 Probe Info  		20.00  LDD0466  [18]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0259  AC14 HEK-293T C51(1.11)  LDD1512  [19]
 LDCM0270  AC15 HEK-293T C51(1.00)  LDD1513  [19]
 LDCM0282  AC22 HEK-293T C51(0.95)  LDD1521  [19]
 LDCM0283  AC23 HEK-293T C51(1.16)  LDD1522  [19]
 LDCM0284  AC24 HEK-293T C209(0.92)  LDD1523  [19]
 LDCM0291  AC30 HEK-293T C51(0.88)  LDD1530  [19]
 LDCM0292  AC31 HEK-293T C51(1.32)  LDD1531  [19]
 LDCM0293  AC32 HEK-293T C209(1.17)  LDD1532  [19]
 LDCM0299  AC38 HEK-293T C51(1.00)  LDD1538  [19]
 LDCM0300  AC39 HEK-293T C51(1.07)  LDD1539  [19]
 LDCM0302  AC40 HEK-293T C209(0.99)  LDD1541  [19]
 LDCM0308  AC46 HEK-293T C51(0.69)  LDD1547  [19]
 LDCM0309  AC47 HEK-293T C51(1.07)  LDD1548  [19]
 LDCM0310  AC48 HEK-293T C209(0.94)  LDD1549  [19]
 LDCM0317  AC54 HEK-293T C51(1.00)  LDD1556  [19]
 LDCM0318  AC55 HEK-293T C51(0.95)  LDD1557  [19]
 LDCM0319  AC56 HEK-293T C209(1.00)  LDD1558  [19]
 LDCM0323  AC6 HEK-293T C51(1.00)  LDD1562  [19]
 LDCM0326  AC62 HEK-293T C51(0.78)  LDD1565  [19]
 LDCM0327  AC63 HEK-293T C51(1.15)  LDD1566  [19]
 LDCM0328  AC64 HEK-293T C209(1.13)  LDD1567  [19]
 LDCM0334  AC7 HEK-293T C51(0.91)  LDD1568  [19]
 LDCM0345  AC8 HEK-293T C209(0.89)  LDD1569  [19]
 LDCM0275  AKOS034007705 HEK-293T C209(0.98)  LDD1514  [19]
 LDCM0108  Chloroacetamide HeLa C51(0.00); H157(0.00); C209(0.00)  LDD0222  [8]
 LDCM0367  CL1 HEK-293T C51(1.03)  LDD1571  [19]
 LDCM0368  CL10 HEK-293T C51(1.27)  LDD1572  [19]
 LDCM0370  CL101 HEK-293T C51(1.03)  LDD1574  [19]
 LDCM0374  CL105 HEK-293T C51(0.90)  LDD1578  [19]
 LDCM0378  CL109 HEK-293T C51(1.01)  LDD1582  [19]
 LDCM0379  CL11 HEK-293T C51(0.96)  LDD1583  [19]
 LDCM0383  CL113 HEK-293T C51(0.98)  LDD1587  [19]
 LDCM0387  CL117 HEK-293T C51(1.20)  LDD1591  [19]
 LDCM0390  CL12 HEK-293T C209(0.84)  LDD1594  [19]
 LDCM0392  CL121 HEK-293T C51(1.20)  LDD1596  [19]
 LDCM0396  CL125 HEK-293T C51(0.98)  LDD1600  [19]
 LDCM0400  CL13 HEK-293T C51(0.95)  LDD1604  [19]
 LDCM0410  CL22 HEK-293T C51(0.92)  LDD1614  [19]
 LDCM0411  CL23 HEK-293T C51(1.09)  LDD1615  [19]
 LDCM0412  CL24 HEK-293T C209(0.96)  LDD1616  [19]
 LDCM0413  CL25 HEK-293T C51(1.10)  LDD1617  [19]
 LDCM0423  CL34 HEK-293T C51(0.93)  LDD1627  [19]
 LDCM0424  CL35 HEK-293T C51(1.01)  LDD1628  [19]
 LDCM0425  CL36 HEK-293T C209(0.75)  LDD1629  [19]
 LDCM0426  CL37 HEK-293T C51(0.85)  LDD1630  [19]
 LDCM0436  CL46 HEK-293T C51(0.93)  LDD1640  [19]
 LDCM0437  CL47 HEK-293T C51(0.95)  LDD1641  [19]
 LDCM0438  CL48 HEK-293T C209(0.91)  LDD1642  [19]
 LDCM0439  CL49 HEK-293T C51(1.17)  LDD1643  [19]
 LDCM0449  CL58 HEK-293T C51(1.18)  LDD1652  [19]
 LDCM0450  CL59 HEK-293T C51(0.97)  LDD1653  [19]
 LDCM0452  CL60 HEK-293T C209(0.86)  LDD1655  [19]
 LDCM0453  CL61 HEK-293T C51(1.21)  LDD1656  [19]
 LDCM0463  CL70 HEK-293T C51(0.88)  LDD1666  [19]
 LDCM0464  CL71 HEK-293T C51(1.20)  LDD1667  [19]
 LDCM0465  CL72 HEK-293T C209(0.90)  LDD1668  [19]
 LDCM0466  CL73 HEK-293T C51(0.95)  LDD1669  [19]
 LDCM0476  CL82 HEK-293T C51(0.83)  LDD1679  [19]
 LDCM0477  CL83 HEK-293T C51(1.08)  LDD1680  [19]
 LDCM0478  CL84 HEK-293T C209(0.78)  LDD1681  [19]
 LDCM0479  CL85 HEK-293T C51(0.93)  LDD1682  [19]
 LDCM0489  CL94 HEK-293T C51(0.89)  LDD1692  [19]
 LDCM0490  CL95 HEK-293T C51(1.15)  LDD1693  [19]
 LDCM0491  CL96 HEK-293T C209(0.91)  LDD1694  [19]
 LDCM0492  CL97 HEK-293T C51(0.90)  LDD1695  [19]
 LDCM0116  HHS-0101 DM93 Y140(0.91)  LDD0264  [6]
 LDCM0117  HHS-0201 DM93 Y140(1.60)  LDD0265  [6]
 LDCM0118  HHS-0301 DM93 Y140(1.99)  LDD0266  [6]
 LDCM0119  HHS-0401 DM93 Y140(2.08)  LDD0267  [6]
 LDCM0120  HHS-0701 DM93 Y140(2.32)  LDD0268  [6]
 LDCM0107  IAA HeLa H157(0.00); C209(0.00)  LDD0221  [8]
 LDCM0123  JWB131 DM93 Y140(1.22)  LDD0285  [5]
 LDCM0124  JWB142 DM93 Y140(1.38)  LDD0286  [5]
 LDCM0125  JWB146 DM93 Y140(1.20)  LDD0287  [5]
 LDCM0126  JWB150 DM93 Y140(2.55)  LDD0288  [5]
 LDCM0127  JWB152 DM93 Y140(1.92)  LDD0289  [5]
 LDCM0128  JWB198 DM93 Y140(0.19)  LDD0290  [5]
 LDCM0129  JWB202 DM93 Y140(1.14)  LDD0291  [5]
 LDCM0130  JWB211 DM93 Y140(1.11)  LDD0292  [5]
 LDCM0022  KB02 OPM-2 C35(1.28)  LDD2543  [4]
 LDCM0023  KB03 OPM-2 C35(1.25)  LDD2960  [4]
 LDCM0024  KB05 OPM-2 C35(1.25)  LDD3377  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Diamine oxidase [copper-containing] (AOC1) Copper/topaquinone oxidase family P19801
Helicase MOV-10 (MOV10) DNA2/NAM7 helicase family Q9HCE1
Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A) CAMK Ser/Thr protein kinase family Q9UQM7
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein lifeguard 1 (GRINA) BI1 family Q7Z429
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Krueppel-like factor 1 (KLF1) Krueppel C2H2-type zinc-finger protein family Q13351
Other
Click To Hide/Show 14 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
A-kinase anchor protein 8-like (AKAP8L) AKAP95 family Q9ULX6
BPI fold-containing family A member 1 (BPIFA1) Plunc family Q9NP55
Protein INCA1 (INCA1) INCA family Q0VD86
Keratin-associated protein 8-1 (KRTAP8-1) KRTAP type 8 family Q8IUC2
MAPK-interacting and spindle-stabilizing protein-like (MAPK1IP1L) MISS family Q8NDC0
Proline-rich protein 20D (PRR20D) PRR20 family P86480
U1 small nuclear ribonucleoprotein A (SNRPA) RRM U1 A/B'' family P09012
Vacuolar protein sorting-associated protein 37C (VPS37C) VPS37 family A5D8V6
DAZ-associated protein 2 (DAZAP2) . Q15038
KH domain-containing RNA-binding protein QKI (QKI) . Q96PU8
Melanoma-associated antigen D1 (MAGED1) . Q9Y5V3
Proline-rich protein 13 (PRR13) . Q9NZ81
RNA-binding protein with multiple splicing 2 (RBPMS2) . Q6ZRY4
Uncharacterized protein C10orf55 (C10orf55) . Q5SWW7

References

1 A Chemical Proteomic Analysis of Illudin-Interacting Proteins. Chemistry. 2019 Sep 25;25(54):12644-12651. doi: 10.1002/chem.201902919. Epub 2019 Sep 3.
Mass spectrometry data entry: PXD014175
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
12 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
13 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
14 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
15 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
16 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
17 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
18 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
19 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402