Details of the Target

General Information of Target

Target ID LDTP02279
Target Name Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1)
Gene Name PDHA1
Gene ID 5160
Synonyms
PHE1A; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC 1.2.4.1; PDHE1-A type I
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTRED
GLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRA
HGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALA
CKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAAST
DYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVS
YRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPP
LEELGYHIYSSDPPFEVRGANQWIKFKSVS
Target Bioclass
Enzyme
Subcellular location
Mitochondrion matrix
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Uniprot ID
P08559
Ensemble ID
ENST00000355808.10
HGNC ID
HGNC:8806

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
NB1 SNV: p.A236V DBIA    Probe Info 
YSCCC SNV: p.R22K DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 38 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		10.18  LDD0402  [1]
N1
 Probe Info  		100.00  LDD0242  [2]
TH211
 Probe Info  		Y118(20.00); Y227(20.00); Y301(20.00); Y156(13.01)  LDD0257  [3]
TH214
 Probe Info  		Y243(7.62)  LDD0258  [3]
TH216
 Probe Info  		Y227(20.00)  LDD0259  [3]
YN-1
 Probe Info  		100.00  LDD0444  [4]
STPyne
 Probe Info  		K18(7.14); K313(8.93); K321(9.71); K385(10.00)  LDD0277  [5]
ONAyne
 Probe Info  		N.A.  LDD0273  [5]
Probe 1
 Probe Info  		Y82(29.77); Y156(8.93); Y227(58.05); Y272(14.51)  LDD3495  [6]
BTD
 Probe Info  		C273(1.80); C41(1.57)  LDD1700  [7]
AHL-Pu-1
 Probe Info  		C273(6.24)  LDD0170  [8]
EA-probe
 Probe Info  		C181(0.88)  LDD2210  [9]
DBIA
 Probe Info  		C261(4.31)  LDD0209  [10]
HHS-482
 Probe Info  		Y156(0.59); Y227(0.71)  LDD0285  [11]
5E-2FA
 Probe Info  		N.A.  LDD2235  [12]
ATP probe
 Probe Info  		K149(0.00); K385(0.00); K83(0.00); K85(0.00)  LDD0199  [13]
4-Iodoacetamidophenylacetylene
 Probe Info  		C273(0.00); C181(0.00); C261(0.00); C218(0.00)  LDD0038  [14]
IA-alkyne
 Probe Info  		C261(0.00); C273(0.00); C218(0.00)  LDD0032  [15]
Lodoacetamide azide
 Probe Info  		C273(0.00); C181(0.00); C261(0.00); C218(0.00)  LDD0037  [14]
ATP probe
 Probe Info  		N.A.  LDD0035  [16]
IPM
 Probe Info  		N.A.  LDD0025  [17]
JW-RF-010
 Probe Info  		C41(0.00); C273(0.00)  LDD0026  [17]
NAIA_4
 Probe Info  		C181(0.00); C222(0.00)  LDD2226  [18]
TFBX
 Probe Info  		C273(0.00); C218(0.00)  LDD0027  [17]
WYneN
 Probe Info  		N.A.  LDD0021  [19]
NHS
 Probe Info  		K321(0.00); K77(0.00); K244(0.00); K277(0.00)  LDD0010  [19]
SF
 Probe Info  		Y227(0.00); Y272(0.00); Y287(0.00); Y301(0.00)  LDD0028  [20]
Phosphinate-6
 Probe Info  		C91(0.00); C145(0.00)  LDD0018  [21]
1c-yne
 Probe Info  		N.A.  LDD0228  [22]
Acrolein
 Probe Info  		C261(0.00); C181(0.00); C218(0.00); C222(0.00)  LDD0217  [23]
Cinnamaldehyde
 Probe Info  		C261(0.00); C273(0.00)  LDD0220  [23]
Crotonaldehyde
 Probe Info  		H292(0.00); H290(0.00); H121(0.00); C273(0.00)  LDD0219  [23]
Methacrolein
 Probe Info  		C261(0.00); C273(0.00); C41(0.00)  LDD0218  [23]
W1
 Probe Info  		N.A.  LDD0236  [24]
AOyne
 Probe Info  		7.40  LDD0443  [25]
NAIA_5
 Probe Info  		C41(0.00); C273(0.00); C145(0.00); C261(0.00)  LDD2223  [18]
TER-AC
 Probe Info  		N.A.  LDD0426  [26]
HHS-465
 Probe Info  		N.A.  LDD2240  [27]
PAL-AfBPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe13
 Probe Info  		20.00  LDD0475  [28]
FFF probe2
 Probe Info  		5.50  LDD0463  [28]
FFF probe3
 Probe Info  		5.15  LDD0465  [28]
STS-1
 Probe Info  		N.A.  LDD0136  [29]
STS-2
 Probe Info  		N.A.  LDD0138  [29]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C41(0.78)  LDD2142  [7]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C41(1.09); C261(0.72)  LDD2112  [7]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C41(0.76)  LDD2095  [7]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C41(0.97); C273(1.01)  LDD2130  [7]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C41(1.09); C273(1.06); C261(0.93)  LDD2117  [7]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C41(1.04); C273(1.15)  LDD2152  [7]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C41(1.39)  LDD2103  [7]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C41(0.59)  LDD2132  [7]
 LDCM0025  4SU-RNA DM93 C273(6.24)  LDD0170  [8]
 LDCM0545  Acetamide MDA-MB-231 C41(0.42); C273(0.81)  LDD2138  [7]
 LDCM0520  AKOS000195272 MDA-MB-231 C41(0.77); C273(0.99)  LDD2113  [7]
 LDCM0498  BS-3668 MDA-MB-231 C41(0.45); C273(0.82)  LDD2091  [7]
 LDCM0108  Chloroacetamide HeLa C181(0.00); C261(0.00); H292(0.00); C218(0.00)  LDD0222  [23]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C273(2.17); C261(2.00); C41(1.34)  LDD1702  [7]
 LDCM0175  Ethacrynic acid HeLa C181(0.88)  LDD2210  [9]
 LDCM0625  F8 Ramos C273(0.85); C261(0.70); C181(1.10); 0.73  LDD2187  [30]
 LDCM0572  Fragment10 Ramos C273(0.64); C261(0.54); C181(0.54); 0.68  LDD2189  [30]
 LDCM0573  Fragment11 Ramos C273(0.06); C261(0.28); C181(0.12); 0.22  LDD2190  [30]
 LDCM0574  Fragment12 Ramos C273(0.70); C261(0.79); C181(0.55); 0.72  LDD2191  [30]
 LDCM0575  Fragment13 Ramos C273(1.08); C261(1.23); C181(0.98); 0.41  LDD2192  [30]
 LDCM0576  Fragment14 Ramos C273(0.68); C261(0.66); C181(0.64); 0.89  LDD2193  [30]
 LDCM0579  Fragment20 Ramos C273(0.56); C261(0.56); C181(0.56); 0.40  LDD2194  [30]
 LDCM0580  Fragment21 Ramos C273(0.88); C261(1.08); C181(0.88); 0.87  LDD2195  [30]
 LDCM0582  Fragment23 Ramos C273(0.46); C261(0.52); C181(0.74); 0.64  LDD2196  [30]
 LDCM0578  Fragment27 Ramos C273(1.00); C261(1.09); C181(1.07); 1.49  LDD2197  [30]
 LDCM0586  Fragment28 Ramos C273(0.85); C261(0.34); C181(1.30); 0.03  LDD2198  [30]
 LDCM0588  Fragment30 Ramos C273(1.13); C261(1.16); C181(0.70); 0.54  LDD2199  [30]
 LDCM0589  Fragment31 Ramos C273(0.88); C261(1.15); C181(0.78); 1.05  LDD2200  [30]
 LDCM0590  Fragment32 Ramos C273(0.47); C261(0.60); C181(0.47); 0.65  LDD2201  [30]
 LDCM0468  Fragment33 Ramos C273(0.95); C261(0.88); C181(0.65); 0.70  LDD2202  [30]
 LDCM0596  Fragment38 Ramos C273(0.97); C261(0.94); C181(0.93)  LDD2203  [30]
 LDCM0566  Fragment4 Ramos C273(0.84); C261(0.78); C181(0.66); 0.64  LDD2184  [30]
 LDCM0610  Fragment52 Ramos C273(1.28); C261(0.98); C181(0.83); 1.21  LDD2204  [30]
 LDCM0614  Fragment56 Ramos C273(1.02); C261(1.10); C181(0.89); 0.62  LDD2205  [30]
 LDCM0569  Fragment7 Ramos C273(0.64); C261(0.55); C181(0.59); 0.38  LDD2186  [30]
 LDCM0571  Fragment9 Ramos C273(0.51); C261(0.57); C181(0.45); 0.42  LDD2188  [30]
 LDCM0107  IAA HeLa C261(0.00); H292(0.00); C218(0.00); C273(0.00)  LDD0221  [23]
 LDCM0123  JWB131 DM93 Y156(0.59); Y227(0.71)  LDD0285  [11]
 LDCM0124  JWB142 DM93 Y156(0.87); Y227(0.05)  LDD0286  [11]
 LDCM0125  JWB146 DM93 Y156(0.50); Y227(0.05)  LDD0287  [11]
 LDCM0126  JWB150 DM93 Y156(1.51); Y227(4.55)  LDD0288  [11]
 LDCM0127  JWB152 DM93 Y156(1.14); Y227(3.45)  LDD0289  [11]
 LDCM0128  JWB198 DM93 Y156(0.13); Y227(0.12)  LDD0290  [11]
 LDCM0129  JWB202 DM93 Y156(0.64); Y227(0.36)  LDD0291  [11]
 LDCM0130  JWB211 DM93 Y156(0.52); Y227(0.56)  LDD0292  [11]
 LDCM0022  KB02 HEK-293T C273(1.03); C261(0.97); C218(1.15); C222(1.00)  LDD1492  [31]
 LDCM0023  KB03 Jurkat C261(4.31)  LDD0209  [10]
 LDCM0024  KB05 COLO792 C219(0.69); C299(1.28)  LDD3310  [32]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C41(0.58); C273(0.88)  LDD2121  [7]
 LDCM0109  NEM HeLa H292(0.00); C261(0.00); H290(0.00); H121(0.00)  LDD0223  [23]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C41(0.52); C273(1.07)  LDD2089  [7]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C41(0.76)  LDD2092  [7]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C41(1.04); C273(1.19)  LDD2093  [7]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C41(1.14)  LDD2094  [7]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C41(0.60)  LDD2096  [7]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C41(1.23); C273(0.74)  LDD2097  [7]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C41(0.98)  LDD2098  [7]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C41(1.00); C273(1.11); C261(1.12)  LDD2099  [7]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C41(0.70)  LDD2100  [7]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C41(1.07); C273(0.89)  LDD2101  [7]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C41(0.74)  LDD2104  [7]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C41(1.37)  LDD2105  [7]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C41(0.62)  LDD2106  [7]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C41(0.94); C261(1.33)  LDD2107  [7]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C41(0.82); C222(1.06)  LDD2108  [7]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C41(0.83); C273(0.87); C261(0.93)  LDD2109  [7]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C41(1.18)  LDD2110  [7]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C41(0.97); C273(1.27)  LDD2111  [7]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C41(0.76)  LDD2115  [7]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C41(0.93)  LDD2116  [7]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C41(0.83)  LDD2118  [7]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C41(1.62); C273(1.84)  LDD2119  [7]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C41(0.63); C273(1.53)  LDD2120  [7]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C41(0.86)  LDD2122  [7]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C41(1.02); C273(1.19); C261(1.05)  LDD2123  [7]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C41(0.82)  LDD2124  [7]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C41(0.93); C273(0.95); C261(1.06)  LDD2125  [7]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C41(1.01)  LDD2126  [7]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C41(1.15); C273(1.07)  LDD2127  [7]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C41(0.91)  LDD2128  [7]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C41(1.06); C273(1.37)  LDD2129  [7]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C41(0.65); C273(0.81)  LDD2133  [7]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C41(0.62); C273(0.54)  LDD2134  [7]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C41(1.06); C273(1.21)  LDD2135  [7]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C41(1.13); C273(1.32); C261(1.40)  LDD2136  [7]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C41(1.01); C261(1.02); C222(0.91)  LDD2137  [7]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C273(1.80); C41(1.57)  LDD1700  [7]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C41(0.93); C273(0.94); C261(0.95)  LDD2140  [7]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C41(0.95); C261(0.71)  LDD2141  [7]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C41(0.99)  LDD2143  [7]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C41(2.16); C273(1.87)  LDD2144  [7]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C41(1.05); C261(1.01)  LDD2145  [7]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C41(0.85); C273(1.08)  LDD2146  [7]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C41(0.98); C273(0.56)  LDD2148  [7]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C41(1.29)  LDD2149  [7]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C41(0.31); C273(0.63)  LDD2150  [7]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C41(0.84)  LDD2151  [7]
 LDCM0628  OTUB2-COV-1 HEK-293T C181(0.70)  LDD2207  [33]
 LDCM0131  RA190 MM1.R C273(1.52); C261(1.24)  LDD0304  [34]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1) PDK/BCKDK protein kinase family Q15118
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) . P11177
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
MICOS complex subunit MIC60 (IMMT) MICOS complex subunit Mic60 family Q16891

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Nadh Small molecular drug DB00157

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
7 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
8 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
9 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
10 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
11 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
12 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
13 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
14 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
15 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
16 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
17 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
18 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
19 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
20 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
21 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
22 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
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24 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
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27 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
28 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
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Mass spectrometry data entry: PXD027578
31 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
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Mass spectrometry data entry: PXD047840
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