Details of the Target

General Information of Target

Target ID LDTP14239
Target Name Mitotic spindle assembly checkpoint protein MAD1 (MAD1L1)
Gene Name MAD1L1
Gene ID 8379
Synonyms
MAD1; TXBP181; Mitotic spindle assembly checkpoint protein MAD1; Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKA
GSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDST
TPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYN
IYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHE
PESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQ
ATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGT
IEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQ
KDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINA
IKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTS
SFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGM
SNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETIN
RYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYL
QEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSA
LRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHS
PQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQN
VASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAF
SVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQ
KNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSL
TGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAI
VDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGC
NPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCI
AQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSF
QDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVR
GWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKL
PEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSG
TNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEK
PLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIK
CVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLF
KLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMD
ESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHA
SFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ
Target Bioclass
Other
Family
MAD1 family
Subcellular location
Cytoplasm; Nucleus
Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling.; [Isoform 3]: Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas.
Uniprot ID
Q9Y6D9
Ensemble ID
ENST00000265854.12
HGNC ID
HGNC:6762

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2058 SNV: p.R512Q DBIA    Probe Info 
A549 SNV: p.Q188H DBIA    Probe Info 
JHH1 Substitution: p.E274_T275delinsDS DBIA    Probe Info 
KMCH1 SNV: p.S12Y; p.V83A DBIA    Probe Info 
LS180 SNV: p.R617Q DBIA    Probe Info 
MDAMB453 SNV: p.N103I DBIA    Probe Info 
MFE319 SNV: p.M49T DBIA    Probe Info 
NALM6 SNV: p.R630H DBIA    Probe Info 
NB4 SNV: p.G687R .
RH41 SNV: p.F629L DBIA    Probe Info 
SNU1 SNV: p.D661N DBIA    Probe Info 
TOV21G Deletion: p.E437RfsTer41 DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		10.73  LDD0402  [1]
STPyne
 Probe Info  		K313(6.25); K406(5.19); K619(1.77); K625(1.59)  LDD0277  [2]
IPM
 Probe Info  		C662(0.00); C201(0.00); C134(0.00); C568(0.00)  LDD0241  [3]
Probe 1
 Probe Info  		Y427(9.23)  LDD3495  [4]
BTD
 Probe Info  		C201(1.16)  LDD2101  [5]
DBIA
 Probe Info  		C134(1.04)  LDD0080  [6]
4-Iodoacetamidophenylacetylene
 Probe Info  		C568(0.00); C662(0.00); C134(0.00); C201(0.00)  LDD0038  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [8]
Lodoacetamide azide
 Probe Info  		C662(0.00); C201(0.00); C568(0.00); C134(0.00)  LDD0037  [7]
ENE
 Probe Info  		N.A.  LDD0006  [9]
TFBX
 Probe Info  		C134(0.00); C568(0.00); C201(0.00)  LDD0148  [10]
VSF
 Probe Info  		N.A.  LDD0007  [9]
Acrolein
 Probe Info  		C568(0.00); C662(0.00)  LDD0217  [11]
Crotonaldehyde
 Probe Info  		C568(0.00); H561(0.00)  LDD0219  [11]
Methacrolein
 Probe Info  		C568(0.00); C662(0.00)  LDD0218  [11]
NAIA_5
 Probe Info  		C568(0.00); C662(0.00); C134(0.00)  LDD2223  [12]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C112
 Probe Info  		17.27  LDD1799  [13]
C158
 Probe Info  		8.88  LDD1838  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C201(0.73)  LDD2117  [5]
 LDCM0026  4SU-RNA+native RNA HEK-293T C201(2.11)  LDD0372  [14]
 LDCM0214  AC1 HCT 116 C134(1.18); C201(0.66); C568(0.83)  LDD0531  [6]
 LDCM0215  AC10 PaTu 8988t C633(1.03); C568(1.09)  LDD1094  [6]
 LDCM0216  AC100 HCT 116 C134(0.56); C568(0.72)  LDD0533  [6]
 LDCM0217  AC101 HCT 116 C134(0.57); C568(0.73)  LDD0534  [6]
 LDCM0218  AC102 HCT 116 C134(0.49); C568(0.57)  LDD0535  [6]
 LDCM0219  AC103 HCT 116 C134(0.45); C568(0.69)  LDD0536  [6]
 LDCM0220  AC104 HCT 116 C134(0.44); C568(0.61)  LDD0537  [6]
 LDCM0221  AC105 HCT 116 C134(0.43); C568(0.69)  LDD0538  [6]
 LDCM0222  AC106 HCT 116 C134(0.46); C568(0.72)  LDD0539  [6]
 LDCM0223  AC107 HCT 116 C134(0.61); C568(0.73)  LDD0540  [6]
 LDCM0224  AC108 HCT 116 C134(0.57); C568(0.71)  LDD0541  [6]
 LDCM0225  AC109 HCT 116 C134(0.49); C568(0.69)  LDD0542  [6]
 LDCM0226  AC11 PaTu 8988t C633(0.72); C568(1.11)  LDD1105  [6]
 LDCM0227  AC110 HCT 116 C134(0.51); C568(0.63)  LDD0544  [6]
 LDCM0228  AC111 HCT 116 C134(0.51); C568(0.74)  LDD0545  [6]
 LDCM0229  AC112 HCT 116 C134(0.57); C568(0.69)  LDD0546  [6]
 LDCM0230  AC113 HCT 116 C134(0.95)  LDD0547  [6]
 LDCM0231  AC114 HCT 116 C134(0.83)  LDD0548  [6]
 LDCM0232  AC115 HCT 116 C134(0.69)  LDD0549  [6]
 LDCM0233  AC116 HCT 116 C134(0.71)  LDD0550  [6]
 LDCM0234  AC117 HCT 116 C134(0.83)  LDD0551  [6]
 LDCM0235  AC118 HCT 116 C134(1.06)  LDD0552  [6]
 LDCM0236  AC119 HCT 116 C134(0.88)  LDD0553  [6]
 LDCM0237  AC12 PaTu 8988t C633(0.69); C568(0.92)  LDD1116  [6]
 LDCM0238  AC120 HCT 116 C134(0.75)  LDD0555  [6]
 LDCM0239  AC121 HCT 116 C134(0.87)  LDD0556  [6]
 LDCM0240  AC122 HCT 116 C134(1.03)  LDD0557  [6]
 LDCM0241  AC123 HCT 116 C134(0.90)  LDD0558  [6]
 LDCM0242  AC124 HCT 116 C134(0.98)  LDD0559  [6]
 LDCM0243  AC125 HCT 116 C134(0.88)  LDD0560  [6]
 LDCM0244  AC126 HCT 116 C134(0.82)  LDD0561  [6]
 LDCM0245  AC127 HCT 116 C134(0.75)  LDD0562  [6]
 LDCM0246  AC128 HCT 116 C134(1.00); C568(1.43)  LDD0563  [6]
 LDCM0247  AC129 HCT 116 C134(1.16); C568(1.14)  LDD0564  [6]
 LDCM0249  AC130 HCT 116 C134(0.99); C568(1.38)  LDD0566  [6]
 LDCM0250  AC131 HCT 116 C134(0.91); C568(1.20)  LDD0567  [6]
 LDCM0251  AC132 HCT 116 C134(1.00); C568(1.27)  LDD0568  [6]
 LDCM0252  AC133 HCT 116 C134(0.90); C568(1.25)  LDD0569  [6]
 LDCM0253  AC134 HCT 116 C134(0.93); C568(1.25)  LDD0570  [6]
 LDCM0254  AC135 HCT 116 C134(0.85); C568(1.33)  LDD0571  [6]
 LDCM0255  AC136 HCT 116 C134(0.92); C568(1.16)  LDD0572  [6]
 LDCM0256  AC137 HCT 116 C134(0.93); C568(1.10)  LDD0573  [6]
 LDCM0257  AC138 HCT 116 C134(0.85); C568(1.23)  LDD0574  [6]
 LDCM0258  AC139 HCT 116 C134(0.98); C568(1.36)  LDD0575  [6]
 LDCM0259  AC14 PaTu 8988t C633(1.01); C568(1.05)  LDD1138  [6]
 LDCM0260  AC140 HCT 116 C134(0.90); C568(1.39)  LDD0577  [6]
 LDCM0261  AC141 HCT 116 C134(0.95); C568(1.31)  LDD0578  [6]
 LDCM0262  AC142 HCT 116 C134(1.25); C568(1.39)  LDD0579  [6]
 LDCM0263  AC143 HCT 116 C134(0.81); C568(1.48)  LDD0580  [6]
 LDCM0264  AC144 HCT 116 C134(0.59); C568(0.90)  LDD0581  [6]
 LDCM0265  AC145 HCT 116 C134(0.72); C568(1.07)  LDD0582  [6]
 LDCM0266  AC146 HCT 116 C134(0.63); C568(1.13)  LDD0583  [6]
 LDCM0267  AC147 HCT 116 C134(0.52); C568(0.95)  LDD0584  [6]
 LDCM0268  AC148 HCT 116 C134(0.66); C568(1.10)  LDD0585  [6]
 LDCM0269  AC149 HCT 116 C134(0.59); C568(1.31)  LDD0586  [6]
 LDCM0270  AC15 PaTu 8988t C633(0.97); C568(1.05)  LDD1149  [6]
 LDCM0271  AC150 HCT 116 C134(0.73); C568(0.84)  LDD0588  [6]
 LDCM0272  AC151 HCT 116 C134(0.90); C568(1.19)  LDD0589  [6]
 LDCM0273  AC152 HCT 116 C134(0.59); C568(0.70)  LDD0590  [6]
 LDCM0274  AC153 HCT 116 C134(0.97); C568(1.33)  LDD0591  [6]
 LDCM0621  AC154 HCT 116 C134(0.69); C568(0.81)  LDD2158  [6]
 LDCM0622  AC155 HCT 116 C134(0.70); C568(0.60)  LDD2159  [6]
 LDCM0623  AC156 HCT 116 C134(0.92); C568(0.83)  LDD2160  [6]
 LDCM0624  AC157 HCT 116 C134(0.85); C568(0.65)  LDD2161  [6]
 LDCM0276  AC17 HCT 116 C568(0.78); C201(1.14)  LDD0593  [6]
 LDCM0277  AC18 HCT 116 C568(0.34); C201(1.07)  LDD0594  [6]
 LDCM0278  AC19 HCT 116 C568(0.88); C201(1.10)  LDD0595  [6]
 LDCM0279  AC2 HCT 116 C201(0.85); C134(1.04); C568(1.42)  LDD0596  [6]
 LDCM0280  AC20 HCT 116 C201(1.10); C568(1.17)  LDD0597  [6]
 LDCM0281  AC21 HCT 116 C568(0.89); C201(1.17)  LDD0598  [6]
 LDCM0282  AC22 HCT 116 C568(1.11); C201(1.18)  LDD0599  [6]
 LDCM0283  AC23 HCT 116 C568(0.71); C201(0.97)  LDD0600  [6]
 LDCM0284  AC24 HCT 116 C568(0.88); C201(1.16)  LDD0601  [6]
 LDCM0285  AC25 HCT 116 C568(1.04); C201(1.11)  LDD0602  [6]
 LDCM0286  AC26 HCT 116 C201(1.21); C568(1.33)  LDD0603  [6]
 LDCM0287  AC27 HCT 116 C568(1.16); C201(1.64)  LDD0604  [6]
 LDCM0288  AC28 HCT 116 C568(1.21); C201(1.34)  LDD0605  [6]
 LDCM0289  AC29 HCT 116 C568(1.10); C201(1.29)  LDD0606  [6]
 LDCM0290  AC3 HCT 116 C134(1.09); C201(1.21); C568(1.26)  LDD0607  [6]
 LDCM0291  AC30 HCT 116 C568(1.04); C201(1.23)  LDD0608  [6]
 LDCM0292  AC31 HCT 116 C201(1.15); C568(1.23)  LDD0609  [6]
 LDCM0293  AC32 HCT 116 C201(1.36); C568(1.36)  LDD0610  [6]
 LDCM0294  AC33 HCT 116 C568(1.12); C201(1.36)  LDD0611  [6]
 LDCM0295  AC34 HCT 116 C568(0.90); C201(1.30)  LDD0612  [6]
 LDCM0296  AC35 HCT 116 C201(0.86)  LDD0613  [6]
 LDCM0297  AC36 HCT 116 C201(1.32)  LDD0614  [6]
 LDCM0298  AC37 HCT 116 C201(1.10)  LDD0615  [6]
 LDCM0299  AC38 HCT 116 C201(0.91)  LDD0616  [6]
 LDCM0300  AC39 HCT 116 C201(1.21)  LDD0617  [6]
 LDCM0301  AC4 HCT 116 C134(1.06); C568(1.12); C201(1.23)  LDD0618  [6]
 LDCM0302  AC40 HCT 116 C201(1.65)  LDD0619  [6]
 LDCM0303  AC41 HCT 116 C201(1.06)  LDD0620  [6]
 LDCM0304  AC42 HCT 116 C201(1.18)  LDD0621  [6]
 LDCM0305  AC43 HCT 116 C201(1.30)  LDD0622  [6]
 LDCM0306  AC44 HCT 116 C201(1.18)  LDD0623  [6]
 LDCM0307  AC45 HCT 116 C201(1.76)  LDD0624  [6]
 LDCM0308  AC46 HCT 116 C568(1.16)  LDD0625  [6]
 LDCM0309  AC47 HCT 116 C568(1.36)  LDD0626  [6]
 LDCM0310  AC48 HCT 116 C568(1.50)  LDD0627  [6]
 LDCM0311  AC49 HCT 116 C568(1.29)  LDD0628  [6]
 LDCM0312  AC5 HCT 116 C134(1.00); C201(1.21); C568(1.23)  LDD0629  [6]
 LDCM0313  AC50 HCT 116 C568(1.28)  LDD0630  [6]
 LDCM0314  AC51 HCT 116 C568(1.33)  LDD0631  [6]
 LDCM0315  AC52 HCT 116 C568(1.37)  LDD0632  [6]
 LDCM0316  AC53 HCT 116 C568(1.36)  LDD0633  [6]
 LDCM0317  AC54 HCT 116 C568(1.00)  LDD0634  [6]
 LDCM0318  AC55 HCT 116 C568(1.78)  LDD0635  [6]
 LDCM0319  AC56 HCT 116 C568(2.21)  LDD0636  [6]
 LDCM0320  AC57 HCT 116 C568(1.16); C201(1.25)  LDD0637  [6]
 LDCM0321  AC58 HCT 116 C201(1.39); C568(1.71)  LDD0638  [6]
 LDCM0322  AC59 HCT 116 C568(1.20); C201(1.43)  LDD0639  [6]
 LDCM0323  AC6 PaTu 8988t C633(0.75); C568(0.89)  LDD1202  [6]
 LDCM0324  AC60 HCT 116 C568(1.25); C201(1.46)  LDD0641  [6]
 LDCM0325  AC61 HCT 116 C568(1.36); C201(1.77)  LDD0642  [6]
 LDCM0326  AC62 HCT 116 C568(2.03); C201(3.08)  LDD0643  [6]
 LDCM0327  AC63 HCT 116 C568(1.36); C201(2.15)  LDD0644  [6]
 LDCM0328  AC64 HCT 116 C201(1.20); C568(1.67)  LDD0645  [6]
 LDCM0329  AC65 HCT 116 C201(0.96); C568(1.90)  LDD0646  [6]
 LDCM0330  AC66 HCT 116 C201(0.76); C568(1.60)  LDD0647  [6]
 LDCM0331  AC67 HCT 116 C201(0.98); C568(1.57)  LDD0648  [6]
 LDCM0332  AC68 HCT 116 C134(0.96); C568(1.18); C201(1.54)  LDD0649  [6]
 LDCM0333  AC69 HCT 116 C134(0.98); C568(1.16); C201(1.28)  LDD0650  [6]
 LDCM0334  AC7 PaTu 8988t C633(0.83); C568(0.85)  LDD1213  [6]
 LDCM0335  AC70 HCT 116 C134(0.86); C201(1.11); C568(1.13)  LDD0652  [6]
 LDCM0336  AC71 HCT 116 C134(1.02); C201(1.12); C568(1.20)  LDD0653  [6]
 LDCM0337  AC72 HCT 116 C201(0.94); C568(1.06); C134(1.07)  LDD0654  [6]
 LDCM0338  AC73 HCT 116 C201(1.32); C134(1.40); C568(1.77)  LDD0655  [6]
 LDCM0339  AC74 HCT 116 C201(1.08); C134(1.30); C568(1.41)  LDD0656  [6]
 LDCM0340  AC75 HCT 116 C201(1.42); C134(1.78); C568(1.89)  LDD0657  [6]
 LDCM0341  AC76 HCT 116 C134(0.96); C568(1.23); C201(1.44)  LDD0658  [6]
 LDCM0342  AC77 HCT 116 C134(1.05); C201(1.44); C568(1.57)  LDD0659  [6]
 LDCM0343  AC78 HCT 116 C201(1.32); C568(1.35); C134(1.48)  LDD0660  [6]
 LDCM0344  AC79 HCT 116 C134(1.13); C201(1.21); C568(1.21)  LDD0661  [6]
 LDCM0345  AC8 PaTu 8988t C633(0.80); C568(0.87)  LDD1224  [6]
 LDCM0346  AC80 HCT 116 C134(1.00); C568(1.10); C201(1.13)  LDD0663  [6]
 LDCM0347  AC81 HCT 116 C568(0.82); C201(1.17); C134(1.33)  LDD0664  [6]
 LDCM0348  AC82 HCT 116 C134(1.44); C568(1.69); C201(1.85)  LDD0665  [6]
 LDCM0349  AC83 HCT 116 C134(0.62); C201(1.19); C568(1.31)  LDD0666  [6]
 LDCM0350  AC84 HCT 116 C134(0.54); C568(0.97); C201(1.16)  LDD0667  [6]
 LDCM0351  AC85 HCT 116 C134(0.58); C201(1.07); C568(1.08)  LDD0668  [6]
 LDCM0352  AC86 HCT 116 C134(0.61); C568(1.04); C201(1.19)  LDD0669  [6]
 LDCM0353  AC87 HCT 116 C568(0.87); C134(0.93); C201(1.99)  LDD0670  [6]
 LDCM0354  AC88 HCT 116 C134(0.65); C568(0.98); C201(1.59)  LDD0671  [6]
 LDCM0355  AC89 HCT 116 C134(0.63); C568(0.94); C201(1.60)  LDD0672  [6]
 LDCM0357  AC90 HCT 116 C134(0.75); C568(1.50); C201(1.74)  LDD0674  [6]
 LDCM0358  AC91 HCT 116 C134(0.64); C201(1.21); C568(1.65)  LDD0675  [6]
 LDCM0359  AC92 HCT 116 C134(0.65); C201(0.82); C568(1.10)  LDD0676  [6]
 LDCM0360  AC93 HCT 116 C134(0.64); C201(0.75); C568(0.81)  LDD0677  [6]
 LDCM0361  AC94 HCT 116 C134(0.77); C568(0.87); C201(0.96)  LDD0678  [6]
 LDCM0362  AC95 HCT 116 C134(1.10); C568(1.12); C201(1.22)  LDD0679  [6]
 LDCM0363  AC96 HCT 116 C134(0.65); C201(0.73); C568(1.20)  LDD0680  [6]
 LDCM0364  AC97 HCT 116 C134(0.60); C568(0.91); C201(1.15)  LDD0681  [6]
 LDCM0365  AC98 HCT 116 C134(0.87); C568(0.91)  LDD0682  [6]
 LDCM0366  AC99 HCT 116 C568(0.66); C134(0.76)  LDD0683  [6]
 LDCM0248  AKOS034007472 PaTu 8988t C633(0.89); C568(1.12)  LDD1127  [6]
 LDCM0356  AKOS034007680 PaTu 8988t C633(0.86); C568(1.20)  LDD1235  [6]
 LDCM0275  AKOS034007705 PaTu 8988t C633(0.78); C568(1.13)  LDD1154  [6]
 LDCM0156  Aniline NCI-H1299 12.44  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C201(1.15)  LDD2171  [6]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [11]
 LDCM0632  CL-Sc Hep-G2 C201(0.92); C201(0.77)  LDD2227  [12]
 LDCM0367  CL1 HCT 116 C134(0.86)  LDD0684  [6]
 LDCM0368  CL10 HCT 116 C134(1.23)  LDD0685  [6]
 LDCM0369  CL100 HCT 116 C568(1.10); C134(1.11); C201(1.12)  LDD0686  [6]
 LDCM0370  CL101 PaTu 8988t C633(0.87); C568(0.95)  LDD1249  [6]
 LDCM0371  CL102 PaTu 8988t C633(0.80); C568(0.91)  LDD1250  [6]
 LDCM0372  CL103 PaTu 8988t C633(1.02); C568(1.13)  LDD1251  [6]
 LDCM0373  CL104 PaTu 8988t C633(1.00); C568(0.93)  LDD1252  [6]
 LDCM0374  CL105 HCT 116 C201(1.00); C568(1.00)  LDD0691  [6]
 LDCM0375  CL106 HCT 116 C568(1.14); C201(1.48)  LDD0692  [6]
 LDCM0376  CL107 HCT 116 C568(0.90); C201(1.07)  LDD0693  [6]
 LDCM0377  CL108 HCT 116 C568(0.87); C201(1.25)  LDD0694  [6]
 LDCM0378  CL109 HCT 116 C568(0.79); C201(1.18)  LDD0695  [6]
 LDCM0379  CL11 HCT 116 C134(1.23)  LDD0696  [6]
 LDCM0380  CL110 HCT 116 C568(0.71); C201(1.25)  LDD0697  [6]
 LDCM0381  CL111 HCT 116 C568(0.78); C201(1.19)  LDD0698  [6]
 LDCM0382  CL112 HCT 116 C568(0.88); C201(0.89)  LDD0699  [6]
 LDCM0383  CL113 HCT 116 C568(0.89); C201(1.13)  LDD0700  [6]
 LDCM0384  CL114 HCT 116 C568(1.01); C201(1.11)  LDD0701  [6]
 LDCM0385  CL115 HCT 116 C568(1.00); C201(1.13)  LDD0702  [6]
 LDCM0386  CL116 HCT 116 C201(1.13); C568(1.21)  LDD0703  [6]
 LDCM0387  CL117 HCT 116 C201(2.46)  LDD0704  [6]
 LDCM0388  CL118 HCT 116 C201(0.84)  LDD0705  [6]
 LDCM0389  CL119 HCT 116 C201(1.29)  LDD0706  [6]
 LDCM0390  CL12 HCT 116 C134(1.44)  LDD0707  [6]
 LDCM0391  CL120 HCT 116 C201(1.91)  LDD0708  [6]
 LDCM0392  CL121 HCT 116 C568(0.71)  LDD0709  [6]
 LDCM0393  CL122 HCT 116 C568(1.31)  LDD0710  [6]
 LDCM0394  CL123 HCT 116 C568(1.22)  LDD0711  [6]
 LDCM0395  CL124 HCT 116 C568(1.21)  LDD0712  [6]
 LDCM0396  CL125 HCT 116 C568(1.20); C201(1.47)  LDD0713  [6]
 LDCM0397  CL126 HCT 116 C568(0.88); C201(1.08)  LDD0714  [6]
 LDCM0398  CL127 HCT 116 C568(1.00); C201(1.26)  LDD0715  [6]
 LDCM0399  CL128 HCT 116 C201(1.53); C568(1.57)  LDD0716  [6]
 LDCM0400  CL13 HCT 116 C134(1.16)  LDD0717  [6]
 LDCM0401  CL14 HCT 116 C134(1.43)  LDD0718  [6]
 LDCM0402  CL15 HCT 116 C134(1.09)  LDD0719  [6]
 LDCM0403  CL16 HCT 116 C568(0.93); C201(1.52)  LDD0720  [6]
 LDCM0404  CL17 HCT 116 C568(0.77); C201(0.97)  LDD0721  [6]
 LDCM0405  CL18 HCT 116 C568(0.85); C201(1.10)  LDD0722  [6]
 LDCM0406  CL19 HCT 116 C568(0.90); C201(1.06)  LDD0723  [6]
 LDCM0407  CL2 HCT 116 C134(1.26)  LDD0724  [6]
 LDCM0408  CL20 HCT 116 C568(0.86); C201(1.19)  LDD0725  [6]
 LDCM0409  CL21 HCT 116 C201(1.04); C568(1.07)  LDD0726  [6]
 LDCM0410  CL22 HCT 116 C201(1.18); C568(1.52)  LDD0727  [6]
 LDCM0411  CL23 HCT 116 C568(0.92); C201(1.30)  LDD0728  [6]
 LDCM0412  CL24 HCT 116 C568(1.23); C201(1.30)  LDD0729  [6]
 LDCM0413  CL25 HCT 116 C201(1.37); C568(0.79)  LDD0730  [6]
 LDCM0414  CL26 HCT 116 C201(1.34); C568(0.75)  LDD0731  [6]
 LDCM0415  CL27 HCT 116 C201(1.27); C568(1.08)  LDD0732  [6]
 LDCM0416  CL28 HCT 116 C201(1.31); C568(1.08)  LDD0733  [6]
 LDCM0417  CL29 HCT 116 C201(1.33); C568(1.01)  LDD0734  [6]
 LDCM0418  CL3 HCT 116 C134(1.10)  LDD0735  [6]
 LDCM0419  CL30 HCT 116 C201(1.24); C568(0.95)  LDD0736  [6]
 LDCM0420  CL31 HCT 116 C201(0.88)  LDD0737  [6]
 LDCM0421  CL32 HCT 116 C201(0.98)  LDD0738  [6]
 LDCM0422  CL33 HCT 116 C201(1.21)  LDD0739  [6]
 LDCM0423  CL34 HCT 116 C201(0.87)  LDD0740  [6]
 LDCM0424  CL35 HCT 116 C201(1.06)  LDD0741  [6]
 LDCM0425  CL36 HCT 116 C201(1.03)  LDD0742  [6]
 LDCM0426  CL37 HCT 116 C201(1.20)  LDD0743  [6]
 LDCM0428  CL39 HCT 116 C201(1.02)  LDD0745  [6]
 LDCM0429  CL4 HCT 116 C134(1.22)  LDD0746  [6]
 LDCM0430  CL40 HCT 116 C201(1.07)  LDD0747  [6]
 LDCM0431  CL41 HCT 116 C201(1.08)  LDD0748  [6]
 LDCM0432  CL42 HCT 116 C201(1.01)  LDD0749  [6]
 LDCM0433  CL43 HCT 116 C201(0.98)  LDD0750  [6]
 LDCM0434  CL44 HCT 116 C201(1.22)  LDD0751  [6]
 LDCM0435  CL45 HCT 116 C201(1.26)  LDD0752  [6]
 LDCM0436  CL46 HEK-293T C134(1.05); C201(0.74); C662(0.74)  LDD1034  [6]
 LDCM0437  CL47 HEK-293T C134(0.98); C201(0.94); C662(0.94)  LDD1035  [6]
 LDCM0438  CL48 HEK-293T C134(0.96); C201(0.82); C662(0.82)  LDD1036  [6]
 LDCM0439  CL49 HEK-293T C134(1.24); C201(0.71); C662(0.71)  LDD1037  [6]
 LDCM0440  CL5 HCT 116 C134(1.03)  LDD0757  [6]
 LDCM0441  CL50 HEK-293T C134(1.53); C201(0.39); C662(0.39)  LDD1039  [6]
 LDCM0442  CL51 HEK-293T C134(1.18); C201(0.78); C662(0.78)  LDD1040  [6]
 LDCM0443  CL52 HEK-293T C134(0.76); C201(1.14); C662(1.14)  LDD1041  [6]
 LDCM0444  CL53 HEK-293T C134(0.80); C201(0.73); C662(0.73)  LDD1042  [6]
 LDCM0445  CL54 HEK-293T C134(1.23); C201(0.76); C662(0.76)  LDD1043  [6]
 LDCM0446  CL55 HEK-293T C134(1.37); C201(0.73); C662(0.73)  LDD1044  [6]
 LDCM0447  CL56 HEK-293T C134(0.88); C201(0.70); C662(0.70)  LDD1045  [6]
 LDCM0448  CL57 HEK-293T C134(1.03); C201(0.77); C662(0.77)  LDD1046  [6]
 LDCM0449  CL58 HEK-293T C134(1.17); C201(1.05); C662(1.05)  LDD1047  [6]
 LDCM0450  CL59 HEK-293T C134(1.46); C201(0.49); C662(0.49)  LDD1048  [6]
 LDCM0451  CL6 HCT 116 C134(1.24)  LDD0768  [6]
 LDCM0452  CL60 HEK-293T C134(1.75); C201(0.41); C662(0.41)  LDD1050  [6]
 LDCM0453  CL61 HEK-293T C134(0.95)  LDD1051  [6]
 LDCM0454  CL62 HEK-293T C134(0.92)  LDD1052  [6]
 LDCM0455  CL63 HEK-293T C134(1.01)  LDD1053  [6]
 LDCM0456  CL64 HEK-293T C134(0.75)  LDD1054  [6]
 LDCM0457  CL65 HEK-293T C134(1.22)  LDD1055  [6]
 LDCM0458  CL66 HEK-293T C134(1.03)  LDD1056  [6]
 LDCM0459  CL67 HEK-293T C134(0.97)  LDD1057  [6]
 LDCM0460  CL68 HEK-293T C134(1.31)  LDD1058  [6]
 LDCM0461  CL69 HEK-293T C134(0.90)  LDD1059  [6]
 LDCM0462  CL7 HCT 116 C134(1.27)  LDD0779  [6]
 LDCM0463  CL70 HEK-293T C134(1.05)  LDD1061  [6]
 LDCM0464  CL71 HEK-293T C134(1.21)  LDD1062  [6]
 LDCM0465  CL72 HEK-293T C134(1.03)  LDD1063  [6]
 LDCM0466  CL73 HEK-293T C134(1.26)  LDD1064  [6]
 LDCM0467  CL74 HEK-293T C134(1.69)  LDD1065  [6]
 LDCM0469  CL76 HCT 116 C568(1.28)  LDD0786  [6]
 LDCM0470  CL77 HCT 116 C568(0.98)  LDD0787  [6]
 LDCM0471  CL78 HCT 116 C568(0.99)  LDD0788  [6]
 LDCM0472  CL79 HCT 116 C568(1.32)  LDD0789  [6]
 LDCM0473  CL8 HCT 116 C134(0.91)  LDD0790  [6]
 LDCM0474  CL80 HCT 116 C568(0.97)  LDD0791  [6]
 LDCM0475  CL81 HCT 116 C568(1.19)  LDD0792  [6]
 LDCM0476  CL82 HCT 116 C568(1.62)  LDD0793  [6]
 LDCM0477  CL83 HCT 116 C568(1.52)  LDD0794  [6]
 LDCM0478  CL84 HCT 116 C568(1.75)  LDD0795  [6]
 LDCM0479  CL85 HCT 116 C568(1.22)  LDD0796  [6]
 LDCM0480  CL86 HCT 116 C568(1.07)  LDD0797  [6]
 LDCM0481  CL87 HCT 116 C568(1.37)  LDD0798  [6]
 LDCM0482  CL88 HCT 116 C568(1.44)  LDD0799  [6]
 LDCM0483  CL89 HCT 116 C568(1.73)  LDD0800  [6]
 LDCM0484  CL9 HCT 116 C134(1.30)  LDD0801  [6]
 LDCM0485  CL90 HCT 116 C568(1.07)  LDD0802  [6]
 LDCM0486  CL91 HCT 116 C134(1.28); C201(0.81); C568(1.39)  LDD0803  [6]
 LDCM0487  CL92 HCT 116 C134(1.02); C201(0.89); C568(0.99)  LDD0804  [6]
 LDCM0488  CL93 HCT 116 C134(1.03); C201(0.68); C568(1.27)  LDD0805  [6]
 LDCM0489  CL94 HCT 116 C134(1.14); C201(1.05); C568(1.28)  LDD0806  [6]
 LDCM0490  CL95 HCT 116 C134(0.92); C201(1.05); C568(1.09)  LDD0807  [6]
 LDCM0491  CL96 HCT 116 C134(1.06); C201(0.84); C568(1.07)  LDD0808  [6]
 LDCM0492  CL97 HCT 116 C134(1.07); C201(0.79); C568(1.15)  LDD0809  [6]
 LDCM0493  CL98 HCT 116 C134(1.06); C201(1.59); C568(1.28)  LDD0810  [6]
 LDCM0494  CL99 HCT 116 C134(1.04); C201(0.96); C568(0.97)  LDD0811  [6]
 LDCM0495  E2913 HEK-293T C201(0.92); C568(1.03); C134(1.08); C662(1.16)  LDD1698  [15]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C662(2.73)  LDD1702  [5]
 LDCM0468  Fragment33 HEK-293T C134(1.82)  LDD1066  [6]
 LDCM0427  Fragment51 HCT 116 C201(0.70)  LDD0744  [6]
 LDCM0107  IAA HeLa N.A.  LDD0221  [11]
 LDCM0022  KB02 HCT 116 C134(1.04)  LDD0080  [6]
 LDCM0023  KB03 HCT 116 C134(1.04)  LDD0081  [6]
 LDCM0024  KB05 HCT 116 C134(1.06)  LDD0082  [6]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C201(1.16)  LDD2101  [5]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C201(0.26)  LDD2104  [5]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C201(0.49)  LDD2109  [5]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C201(0.34)  LDD2115  [5]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C134(0.96)  LDD2120  [5]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C201(0.65)  LDD2123  [5]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C201(0.56)  LDD2125  [5]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C134(1.04)  LDD2128  [5]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C201(1.20)  LDD2135  [5]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C201(0.93)  LDD2136  [5]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C201(0.73)  LDD2137  [5]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C201(0.74)  LDD2140  [5]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C134(0.79)  LDD2143  [5]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C201(0.95)  LDD2146  [5]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C134(1.62)  LDD2147  [5]
 LDCM0021  THZ1 HCT 116 C201(1.15)  LDD2173  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Polyunsaturated fatty acid 5-lipoxygenase (ALOX5) Lipoxygenase family P09917
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Ubiquitin carboxyl-terminal hydrolase 15 (USP15) Peptidase C19 family Q9Y4E8
Calpain-7 (CAPN7) Peptidase C2 family Q9Y6W3
Proteasome subunit alpha type-1 (PSMA1) Peptidase T1A family P25786
Cyclin-dependent kinase 18 (CDK18) CMGC Ser/Thr protein kinase family Q07002
E3 ubiquitin-protein ligase RNF8 (RNF8) RNF8 family O76064
Centromere-associated protein E (CENPE) Kinesin family Q02224
Kinesin-like protein KIF9 (KIF9) Kinesin family Q9HAQ2
E3 ubiquitin-protein ligase LNX (LNX1) . Q8TBB1
Tripartite motif-containing protein 29 (TRIM29) . Q14134
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Hsp90 co-chaperone Cdc37 (CDC37) CDC37 family Q16543
Nucleoprotein TPR (TPR) TPR family P12270
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and SCAN domain-containing protein 32 (ZSCAN32) Krueppel C2H2-type zinc-finger protein family Q9NX65
Teashirt homolog 3 (TSHZ3) Teashirt C2H2-type zinc-finger protein family Q63HK5
TSC22 domain family protein 3 (TSC22D3) TSC-22/Dip/Bun family Q99576
Other
Click To Hide/Show 22 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Autophagy protein 5 (ATG5) ATG5 family Q9H1Y0
Testis-specific gene 10 protein (TSGA10) CEP135/TSGA10 family Q9BZW7
Splicing factor YJU2 (YJU2) CWC16 family Q9BW85
CWF19-like protein 2 (CWF19L2) CWF19 family Q2TBE0
Dynein regulatory complex subunit 4 (GAS8) DRC4 family O95995
Protein FAM90A1 (FAM90A1) FAM90 family Q86YD7
HAUS augmin-like complex subunit 1 (HAUS1) HAUS1 family Q96CS2
Mitotic spindle assembly checkpoint protein MAD1 (MAD1L1) MAD1 family Q9Y6D9
Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) MAD2 family Q13257
Microfibrillar-associated protein 1 (MFAP1) MFAP1 family P55081
Mitochondria-eating protein (SPATA18) MIEAP family Q8TC71
G-patch domain and KOW motifs-containing protein (GPKOW) MOS2 family Q92917
Spermatogenesis-associated protein 2 (SPATA2) SPATA2 family Q9UM82
Synaptotagmin-17 (SYT17) Synaptotagmin family Q9BSW7
Tropomyosin alpha-1 chain (TPM1) Tropomyosin family P09493
Tropomyosin alpha-3 chain (TPM3) Tropomyosin family P06753
BAG family molecular chaperone regulator 5 (BAG5) . Q9UL15
Galectin-related protein (LGALSL) . Q3ZCW2
Leukocyte receptor cluster member 1 (LENG1) . Q96BZ8
Nebulette (NEBL) . O76041
Phostensin (PPP1R18) . Q6NYC8
Rhombotin-1 (LMO1) . P25800

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
9 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
12 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
13 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
14 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
15 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402