Details of the Target

General Information of Target

Target ID LDTP10919
Target Name Eukaryotic translation initiation factor 3 subunit C (EIF3C)
Gene Name EIF3C
Gene ID 8663
Synonyms
EIF3S8; Eukaryotic translation initiation factor 3 subunit C; eIF3c; Eukaryotic translation initiation factor 3 subunit 8; eIF3 p110
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDVLPMCSIFQELQIVHETGYFSALPSLEEYWQQTCLELERYLQSEPCYVSASEIKFDSQ
EDLWTKIILAREKKEESELKISSSPPEDTLISPSFCYNLETNSLNSDVSSESSDSSEELS
PTAKFTSDPIGEVLVSSGKLSSSVTSTPPSSPELSREPSQLWGCVPGELPSPGKVRSGTS
GKPGDKGNGDASPDGRRRVHRCHFNGCRKVYTKSSHLKAHQRTHTGEKPYRCSWEGCEWR
FARSDELTRHFRKHTGAKPFKCSHCDRCFSRSDHLALHMKRHL
Target Bioclass
Other
Family
EIF-3 subunit C family
Subcellular location
Cytoplasm
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. {|HAMAP-Rule:MF_03002}.
Uniprot ID
Q99613
Ensemble ID
ENST00000331666.11
HGNC ID
HGNC:3279

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 32 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
CY-1
 Probe Info  		10.10  LDD0243  [1]
CY4
 Probe Info  		6.15  LDD0244  [1]
N1
 Probe Info  		15.90  LDD0242  [1]
TH211
 Probe Info  		Y881(17.66)  LDD0257  [2]
YN-4
 Probe Info  		100.00  LDD0445  [3]
STPyne
 Probe Info  		K136(10.00); K160(2.94); K312(10.00); K331(10.00)  LDD0277  [4]
BTD
 Probe Info  		C752(1.90)  LDD1699  [5]
ONAyne
 Probe Info  		N.A.  LDD0273  [4]
IPM
 Probe Info  		C443(0.00); C79(0.00); C752(0.00)  LDD0241  [6]
AHL-Pu-1
 Probe Info  		C443(2.39)  LDD0168  [7]
HHS-482
 Probe Info  		Y884(0.90)  LDD0285  [8]
HHS-475
 Probe Info  		Y881(0.81); Y884(0.93)  LDD0264  [9]
HHS-465
 Probe Info  		Y881(10.00); Y884(10.00)  LDD2237  [10]
DBIA
 Probe Info  		C443(1.05)  LDD0078  [11]
5E-2FA
 Probe Info  		H155(0.00); H876(0.00)  LDD2235  [12]
AMP probe
 Probe Info  		K643(0.00); K331(0.00); K332(0.00)  LDD0200  [13]
ATP probe
 Probe Info  		K643(0.00); K331(0.00); K332(0.00); K136(0.00)  LDD0199  [13]
m-APA
 Probe Info  		H155(0.00); H876(0.00)  LDD2231  [12]
IA-alkyne
 Probe Info  		C500(0.00); C443(0.00); C79(0.00); C752(0.00)  LDD0032  [14]
IPIAA_H
 Probe Info  		C79(0.00); C443(0.00); C480(0.00)  LDD0030  [15]
IPIAA_L
 Probe Info  		C480(0.00); C79(0.00); C443(0.00)  LDD0031  [15]
NAIA_4
 Probe Info  		C619(0.00); C752(0.00)  LDD2226  [16]
NAIA_5
 Probe Info  		C443(0.00); C444(0.00)  LDD2225  [16]
Compound 10
 Probe Info  		C443(0.00); C79(0.00)  LDD2216  [17]
Compound 11
 Probe Info  		N.A.  LDD2213  [17]
PF-06672131
 Probe Info  		N.A.  LDD0017  [18]
Ox-W18
 Probe Info  		N.A.  LDD2175  [19]
Acrolein
 Probe Info  		H630(0.00); H326(0.00); C752(0.00); C500(0.00)  LDD0217  [20]
Cinnamaldehyde
 Probe Info  		N.A.  LDD0220  [20]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [20]
Methacrolein
 Probe Info  		C752(0.00); C619(0.00); H736(0.00); C443(0.00)  LDD0218  [20]
W1
 Probe Info  		C752(0.00); C500(0.00); C480(0.00)  LDD0236  [6]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		N.A.  LDD0138  [21]
VE-P
 Probe Info  		N.A.  LDD0396  [22]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C752(0.70)  LDD2142  [5]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C752(0.94)  LDD2112  [5]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C752(0.91)  LDD2130  [5]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C500(1.59); C752(1.52)  LDD2117  [5]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C500(1.74); C752(1.49)  LDD2152  [5]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C752(0.70)  LDD2132  [5]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C752(0.54)  LDD2131  [5]
 LDCM0025  4SU-RNA HEK-293T C443(2.39)  LDD0168  [7]
 LDCM0026  4SU-RNA+native RNA HEK-293T C443(2.39)  LDD0169  [7]
 LDCM0545  Acetamide MDA-MB-231 C752(0.57)  LDD2138  [5]
 LDCM0520  AKOS000195272 MDA-MB-231 C500(1.03); C752(0.85)  LDD2113  [5]
 LDCM0020  ARS-1620 HCC44 C443(1.05)  LDD0078  [11]
 LDCM0498  BS-3668 MDA-MB-231 C500(0.60); C752(0.78)  LDD2091  [5]
 LDCM0108  Chloroacetamide HeLa H630(0.00); C619(0.00); C752(0.00); C79(0.00)  LDD0222  [20]
 LDCM0632  CL-Sc Hep-G2 C752(20.00); C443(2.53); C79(0.80); C619(0.66)  LDD2227  [16]
 LDCM0634  CY-0357 Hep-G2 C752(1.85)  LDD2228  [16]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C79(1.73); C443(1.67)  LDD1702  [5]
 LDCM0116  HHS-0101 DM93 Y881(0.81); Y884(0.93)  LDD0264  [9]
 LDCM0117  HHS-0201 DM93 Y881(0.57); Y884(0.78)  LDD0265  [9]
 LDCM0118  HHS-0301 DM93 Y881(0.64); Y884(0.71)  LDD0266  [9]
 LDCM0119  HHS-0401 DM93 Y881(0.60); Y884(0.65)  LDD0267  [9]
 LDCM0120  HHS-0701 DM93 Y881(0.73); Y884(0.74)  LDD0268  [9]
 LDCM0107  IAA HeLa H630(0.00); H326(0.00); H155(0.00); C500(0.00)  LDD0221  [20]
 LDCM0123  JWB131 DM93 Y884(0.90)  LDD0285  [8]
 LDCM0124  JWB142 DM93 Y884(0.61)  LDD0286  [8]
 LDCM0125  JWB146 DM93 Y884(1.04)  LDD0287  [8]
 LDCM0126  JWB150 DM93 Y884(3.15)  LDD0288  [8]
 LDCM0127  JWB152 DM93 Y884(1.84)  LDD0289  [8]
 LDCM0128  JWB198 DM93 Y884(0.95)  LDD0290  [8]
 LDCM0129  JWB202 DM93 Y884(0.36)  LDD0291  [8]
 LDCM0130  JWB211 DM93 Y884(0.95)  LDD0292  [8]
 LDCM0023  KB03 MDA-MB-231 C79(4.25); C443(1.95)  LDD1701  [5]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C500(0.68); C752(0.80)  LDD2121  [5]
 LDCM0109  NEM HeLa H630(0.00); H326(0.00); H155(0.00); H715(0.00)  LDD0223  [20]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C500(0.56)  LDD2089  [5]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C500(0.89)  LDD2090  [5]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C752(0.80)  LDD2092  [5]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C500(0.97); C752(0.99)  LDD2093  [5]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C752(0.92)  LDD2094  [5]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C752(0.32)  LDD2096  [5]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C752(1.03)  LDD2097  [5]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C752(0.77)  LDD2098  [5]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C500(1.92)  LDD2099  [5]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C752(0.72)  LDD2100  [5]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C500(0.95)  LDD2101  [5]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C752(0.80)  LDD2104  [5]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C752(1.22)  LDD2105  [5]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C500(1.57); C480(0.93)  LDD2107  [5]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C752(0.79)  LDD2108  [5]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C500(0.63); C752(0.72)  LDD2109  [5]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C752(0.93)  LDD2110  [5]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C500(1.85); C752(1.00)  LDD2111  [5]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C752(0.69)  LDD2114  [5]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C500(0.63); C752(0.88)  LDD2115  [5]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C752(0.53)  LDD2116  [5]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C500(0.75); C752(0.54)  LDD2118  [5]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C500(0.57); C752(1.35)  LDD2119  [5]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C752(0.74)  LDD2120  [5]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C500(0.59); C752(0.46)  LDD2122  [5]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C500(1.04); C752(0.92)  LDD2123  [5]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C500(0.86); C752(0.63)  LDD2124  [5]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C752(0.93)  LDD2125  [5]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C752(0.87)  LDD2126  [5]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C500(1.35)  LDD2127  [5]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C752(0.77)  LDD2128  [5]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C500(1.26); C752(1.11)  LDD2129  [5]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C752(0.68)  LDD2133  [5]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C500(0.45); C752(0.75)  LDD2134  [5]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C500(1.56); C752(1.35)  LDD2135  [5]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C500(0.75); C752(1.01)  LDD2136  [5]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C500(1.13); C752(1.02)  LDD2137  [5]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C500(3.79); C752(1.85)  LDD1700  [5]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C500(1.13); C752(0.99)  LDD2140  [5]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C752(0.67)  LDD2141  [5]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C500(1.04); C752(0.88)  LDD2143  [5]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C500(1.86); C752(1.28)  LDD2144  [5]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C500(1.47); C752(1.02)  LDD2146  [5]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C752(1.90)  LDD2147  [5]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C500(0.49); C752(0.64)  LDD2148  [5]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C752(0.59)  LDD2149  [5]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C752(0.61)  LDD2150  [5]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C752(0.36)  LDD2151  [5]
 LDCM0627  NUDT7-COV-1 HEK-293T C752(0.61); C619(0.51)  LDD2206  [23]
 LDCM0628  OTUB2-COV-1 HEK-293T C619(1.01); C752(0.86); C443(0.70)  LDD2207  [23]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATP-dependent RNA helicase DDX3X (DDX3X) DEAD box helicase family O00571
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Eukaryotic translation initiation factor 1A, X-chromosomal (EIF1AX) EIF-1A family P47813
Eukaryotic translation initiation factor 3 subunit A (EIF3A) EIF-3 subunit A family Q14152
Eukaryotic translation initiation factor 3 subunit E (EIF3E) EIF-3 subunit E family P60228
Eukaryotic translation initiation factor 1 (EIF1) SUI1 family P41567
Pleckstrin homology domain-containing family F member 2 (PLEKHF2) . Q9H8W4

References

1 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
4 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
7 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
8 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
9 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
10 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
11 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
12 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
13 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
14 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
15 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
16 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
17 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
18 Site-Specific Activity-Based Protein Profiling Using Phosphonate Handles. Mol Cell Proteomics. 2023 Jan;22(1):100455. doi: 10.1016/j.mcpro.2022.100455. Epub 2022 Nov 24.
Mass spectrometry data entry: PXD036569
19 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
20 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
21 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
22 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
23 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.