Details of the Target

General Information of Target

Target ID LDTP06060
Target Name Ubiquitin-associated protein 2-like (UBAP2L)
Gene Name UBAP2L
Gene ID 9898
Synonyms
KIAA0144; NICE4; Ubiquitin-associated protein 2-like; Protein NICE-4; RNA polymerase II degradation factor UBAP2L
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQLIDI
TGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVSGQKDGGQTESNEEG
KENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENGLDGTKSGGPSGRGTERGRRGRGRG
RGGSGRRGGRFSAQGMGTFNPADYAEPANTDDNYGNSSGNTWNNTGHFEPDDGTSAWRTA
TEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQRIDLAVLLGKTPSTMENDSS
NLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVSMLGKGFGDVGEAKGGSTTGS
QFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPSLVQYDLKNPSDSAVHSPFTK
RQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPSKSTSAPQMSPGSSDNQSSSPQP
AQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQFGALQFGSEPVLSDYESTPTTSA
SSSQAPSSLYTSTASESSSTISSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTR
RYPSSISSSPQKDLTQAKNGFSSVQATQLQTTQSVEGATGSAVKSDSPSTSSIPPLNETV
SAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHSSTLSTQQNTLSSSTSSGRTSTSTLLH
TSVESEANLHSSSSTFSTTSSTVSAPPPVVSVSSSLNSGSSLGLSLGSNSTVTASTRSSV
ATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHAYPPQVYGYDDLQMLQTRFPLDYYSIPFP
TPTTPLTGRDGSLASNPYSGDLTKFGRGDASSPAPATTLAQPQQNQTQTHHTTQQTFLNP
ALPPGYSYTSLPYYTGVPGLPSTFQYGPAVFPVAPTSSKQHGVNVSVNASATPFQQPSGY
GSHGYNTGVSVTSSNTGVPDISGSVYSKTQQSFEKQGFHSGTPAASFNLPSALGSGGPIN
PATAAAYPPAPFMHILTPHQQPHSQILHHHLQQDGQTGSGQRSQTSSIPQKPQTNKSAYN
SYSWGAN
Target Bioclass
Other
Subcellular location
Nucleus
Function
Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled nucleotide excision repair (TC-NER) machinery fails to resolve DNA damage. Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT-HSCs). Required for efficient formation of stress granules.
Uniprot ID
Q14157
Ensemble ID
ENST00000343815.10
HGNC ID
HGNC:29877
ChEMBL ID
CHEMBL4295819

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 18 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		8.70  LDD0402  [1]
TH211
 Probe Info  		Y858(10.05)  LDD0257  [2]
TH216
 Probe Info  		Y858(18.18)  LDD0259  [2]
STPyne
 Probe Info  		K101(1.23); K353(7.69); K420(10.00); K864(9.87)  LDD0277  [3]
Probe 1
 Probe Info  		Y858(61.85)  LDD3495  [4]
BTD
 Probe Info  		C68(0.65)  LDD2118  [5]
HHS-482
 Probe Info  		Y858(0.50)  LDD0285  [6]
HHS-475
 Probe Info  		Y858(0.89); Y834(0.97); Y813(1.00); Y835(1.12)  LDD0264  [7]
HHS-465
 Probe Info  		Y834(4.04); Y835(3.29); Y858(10.00)  LDD2237  [8]
5E-2FA
 Probe Info  		H415(0.00); H335(0.00)  LDD2235  [9]
ATP probe
 Probe Info  		K101(0.00); K102(0.00); K26(0.00); K612(0.00)  LDD0199  [10]
ATP probe
 Probe Info  		N.A.  LDD0035  [11]
IA-alkyne
 Probe Info  		N.A.  LDD0176  [12]
SF
 Probe Info  		Y858(0.00); Y834(0.00)  LDD0028  [13]
1c-yne
 Probe Info  		N.A.  LDD0228  [14]
1d-yne
 Probe Info  		N.A.  LDD0357  [14]
Acrolein
 Probe Info  		H25(0.00); C75(0.00)  LDD0217  [15]
Methacrolein
 Probe Info  		N.A.  LDD0218  [15]
PAL-AfBPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C087
 Probe Info  		7.46  LDD1779  [16]
C145
 Probe Info  		9.25  LDD1827  [16]
C147
 Probe Info  		7.06  LDD1829  [16]
C191
 Probe Info  		11.16  LDD1868  [16]
C249
 Probe Info  		12.47  LDD1922  [16]
FFF probe11
 Probe Info  		12.99  LDD0471  [17]
FFF probe13
 Probe Info  		16.27  LDD0475  [17]
FFF probe14
 Probe Info  		20.00  LDD0477  [17]
FFF probe2
 Probe Info  		18.72  LDD0463  [17]
FFF probe3
 Probe Info  		20.00  LDD0464  [17]
FFF probe6
 Probe Info  		16.88  LDD0482  [17]
JN0003
 Probe Info  		20.00  LDD0469  [17]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.39  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa C68(0.00); C75(0.00)  LDD0222  [15]
 LDCM0116  HHS-0101 DM93 Y858(0.89); Y834(0.97); Y813(1.00); Y835(1.12)  LDD0264  [7]
 LDCM0117  HHS-0201 DM93 Y834(0.81); Y858(0.85); Y835(1.00); Y602(1.05)  LDD0265  [7]
 LDCM0118  HHS-0301 DM93 Y820(0.26); Y803(0.26); Y813(0.31); Y818(0.34)  LDD0266  [7]
 LDCM0119  HHS-0401 DM93 Y834(0.65); Y835(0.79); Y858(0.88); Y602(1.15)  LDD0267  [7]
 LDCM0120  HHS-0701 DM93 Y602(0.98); Y835(1.06); Y858(1.16); Y834(1.51)  LDD0268  [7]
 LDCM0107  IAA HeLa C68(0.00); H25(0.00); C75(0.00)  LDD0221  [15]
 LDCM0123  JWB131 DM93 Y858(0.50)  LDD0285  [6]
 LDCM0124  JWB142 DM93 Y835(3.36); Y858(0.55)  LDD0286  [6]
 LDCM0125  JWB146 DM93 Y835(3.45); Y858(1.47)  LDD0287  [6]
 LDCM0126  JWB150 DM93 Y858(3.07)  LDD0288  [6]
 LDCM0127  JWB152 DM93 Y835(14.75); Y858(2.46)  LDD0289  [6]
 LDCM0128  JWB198 DM93 Y858(0.88)  LDD0290  [6]
 LDCM0129  JWB202 DM93 Y835(3.46); Y858(0.32)  LDD0291  [6]
 LDCM0130  JWB211 DM93 Y858(0.41)  LDD0292  [6]
 LDCM0109  NEM HeLa N.A.  LDD0223  [15]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C68(0.65)  LDD2118  [5]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C68(0.44)  LDD2122  [5]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C68(0.69)  LDD2123  [5]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C68(0.69)  LDD2124  [5]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C68(1.01)  LDD2136  [5]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C68(1.18)  LDD2137  [5]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C68(0.23)  LDD2149  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ras GTPase-activating protein-binding protein 1 (G3BP1) . Q13283
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
RNA-binding protein FXR2 (FXR2) FMR1 family P51116

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
7 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
8 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
9 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
12 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
13 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
14 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
15 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
16 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
17 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.