Details of the Target

General Information of Target

Target ID LDTP04573
Target Name LIM domain kinase 1 (LIMK1)
Gene Name LIMK1
Gene ID 3984
Synonyms
LIMK; LIM domain kinase 1; LIMK-1; EC 2.7.11.1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCDCSASLS
HQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGD
GDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSV
SIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDRILEINGTPIRNVPLDEID
LLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSID
RSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRE
TGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGT
LRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFG
LARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLC
EIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKL
EHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Protein kinase superfamily, TKL Ser/Thr protein kinase family
Subcellular location
Cytoplasm
Function
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development.; [Isoform 3]: Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
TTD ID
T70840
Uniprot ID
P53667
DrugMap ID
TTWL9TY
Ensemble ID
ENST00000336180.7
HGNC ID
HGNC:6613
ChEMBL ID
CHEMBL3836

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
G361 SNV: p.P576L DBIA    Probe Info 
HEPG2 SNV: p.G480R .
IGR1 SNV: p.S534F DBIA    Probe Info 
JURKAT SNV: p.K316M .
MELHO SNV: p.P581L DBIA    Probe Info 
NCIH1155 SNV: p.R200C DBIA    Probe Info 
NCIH1793 SNV: p.C7R .
NCIH358 SNV: p.D531Y DBIA    Probe Info 
SUPT1 SNV: p.S439G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C349(21.61); C297(14.56)  LDD0209  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C466(0.00); C390(0.00)  LDD0037  [2]
IPM
 Probe Info  		C349(0.00); C208(0.00)  LDD2156  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C208(0.90); C349(1.32); C466(1.03); C571(0.80)  LDD0531  [5]
 LDCM0215  AC10 HCT 116 C349(1.27); C571(0.90); C575(0.90); C587(2.36)  LDD0532  [5]
 LDCM0216  AC100 HCT 116 C349(0.79); C571(0.79); C575(0.79)  LDD0533  [5]
 LDCM0217  AC101 HCT 116 C349(0.93); C571(0.88); C575(0.88)  LDD0534  [5]
 LDCM0218  AC102 HCT 116 C349(0.86); C571(0.72); C575(0.72)  LDD0535  [5]
 LDCM0219  AC103 HCT 116 C349(0.92); C571(0.62); C575(0.62)  LDD0536  [5]
 LDCM0220  AC104 HCT 116 C349(0.87); C571(0.82); C575(0.82)  LDD0537  [5]
 LDCM0221  AC105 HCT 116 C349(0.86); C571(0.63); C575(0.63)  LDD0538  [5]
 LDCM0222  AC106 HCT 116 C349(0.87); C571(0.69); C575(0.69)  LDD0539  [5]
 LDCM0223  AC107 HCT 116 C349(0.81); C571(0.75); C575(0.76)  LDD0540  [5]
 LDCM0224  AC108 HCT 116 C349(0.86); C571(0.75); C575(0.91)  LDD0541  [5]
 LDCM0225  AC109 HCT 116 C349(0.81); C571(0.89); C575(0.84)  LDD0542  [5]
 LDCM0226  AC11 HCT 116 C349(1.44); C571(0.85); C575(0.85); C587(1.43)  LDD0543  [5]
 LDCM0227  AC110 HCT 116 C349(0.94); C571(0.64); C575(0.67)  LDD0544  [5]
 LDCM0228  AC111 HCT 116 C349(0.94); C571(0.63); C575(0.75)  LDD0545  [5]
 LDCM0229  AC112 HCT 116 C349(1.03); C571(0.78); C575(0.74)  LDD0546  [5]
 LDCM0230  AC113 HCT 116 C349(1.02); C571(1.19); C575(1.19)  LDD0547  [5]
 LDCM0231  AC114 HCT 116 C349(1.30); C571(1.05); C575(1.05)  LDD0548  [5]
 LDCM0232  AC115 HCT 116 C349(1.58); C571(1.01); C575(1.01)  LDD0549  [5]
 LDCM0233  AC116 HCT 116 C349(1.61); C571(1.03); C575(1.03)  LDD0550  [5]
 LDCM0234  AC117 HCT 116 C349(1.30); C571(1.20); C575(1.20)  LDD0551  [5]
 LDCM0235  AC118 HCT 116 C349(1.28); C571(1.05); C575(1.05)  LDD0552  [5]
 LDCM0236  AC119 HCT 116 C349(1.15); C571(0.89); C575(0.89)  LDD0553  [5]
 LDCM0237  AC12 HCT 116 C349(1.06); C571(1.00); C575(1.00); C587(1.47)  LDD0554  [5]
 LDCM0238  AC120 HCT 116 C349(1.62); C571(1.03); C575(1.03)  LDD0555  [5]
 LDCM0239  AC121 HCT 116 C349(1.17); C571(0.95); C575(0.95)  LDD0556  [5]
 LDCM0240  AC122 HCT 116 C349(1.30); C571(0.97); C575(0.97)  LDD0557  [5]
 LDCM0241  AC123 HCT 116 C349(1.37); C571(0.89); C575(0.89)  LDD0558  [5]
 LDCM0242  AC124 HCT 116 C349(1.34); C571(1.01); C575(1.01)  LDD0559  [5]
 LDCM0243  AC125 HCT 116 C349(1.04); C571(1.14); C575(1.14)  LDD0560  [5]
 LDCM0244  AC126 HCT 116 C349(1.59); C571(0.97); C575(0.97)  LDD0561  [5]
 LDCM0245  AC127 HCT 116 C349(1.91); C571(0.96); C575(0.96)  LDD0562  [5]
 LDCM0246  AC128 HCT 116 C587(1.15)  LDD0563  [5]
 LDCM0247  AC129 HCT 116 C587(0.57)  LDD0564  [5]
 LDCM0249  AC130 HCT 116 C587(0.93)  LDD0566  [5]
 LDCM0250  AC131 HCT 116 C587(0.60)  LDD0567  [5]
 LDCM0251  AC132 HCT 116 C587(1.26)  LDD0568  [5]
 LDCM0252  AC133 HCT 116 C587(1.19)  LDD0569  [5]
 LDCM0253  AC134 HCT 116 C587(1.29)  LDD0570  [5]
 LDCM0254  AC135 HCT 116 C587(1.14)  LDD0571  [5]
 LDCM0255  AC136 HCT 116 C587(1.11)  LDD0572  [5]
 LDCM0256  AC137 HCT 116 C587(1.30)  LDD0573  [5]
 LDCM0257  AC138 HCT 116 C587(1.44)  LDD0574  [5]
 LDCM0258  AC139 HCT 116 C587(1.55)  LDD0575  [5]
 LDCM0259  AC14 HCT 116 C349(1.38); C571(1.01); C575(1.01); C587(1.90)  LDD0576  [5]
 LDCM0260  AC140 HCT 116 C587(1.49)  LDD0577  [5]
 LDCM0261  AC141 HCT 116 C587(1.16)  LDD0578  [5]
 LDCM0262  AC142 HCT 116 C587(1.07)  LDD0579  [5]
 LDCM0263  AC143 HCT 116 C571(0.82); C575(0.82); C349(0.93); C587(1.25)  LDD0580  [5]
 LDCM0264  AC144 HCT 116 C587(0.82); C349(1.35); C571(1.46); C575(1.46)  LDD0581  [5]
 LDCM0265  AC145 HCT 116 C587(0.99); C571(1.18); C575(1.18); C349(1.37)  LDD0582  [5]
 LDCM0266  AC146 HCT 116 C587(1.14); C571(1.18); C575(1.18); C349(1.79)  LDD0583  [5]
 LDCM0267  AC147 HCT 116 C571(1.20); C575(1.20); C587(1.40); C349(1.62)  LDD0584  [5]
 LDCM0268  AC148 HCT 116 C587(0.97); C571(1.18); C575(1.18); C349(2.01)  LDD0585  [5]
 LDCM0269  AC149 HCT 116 C587(1.05); C571(1.10); C575(1.10); C349(1.86)  LDD0586  [5]
 LDCM0270  AC15 HCT 116 C571(0.94); C575(0.94); C349(1.38); C587(1.78)  LDD0587  [5]
 LDCM0271  AC150 HCT 116 C587(1.04); C571(1.06); C575(1.06); C349(1.21)  LDD0588  [5]
 LDCM0272  AC151 HCT 116 C587(0.83); C571(0.91); C575(0.91); C349(1.05)  LDD0589  [5]
 LDCM0273  AC152 HCT 116 C587(1.01); C571(1.05); C575(1.05); C349(2.08)  LDD0590  [5]
 LDCM0274  AC153 HCT 116 C587(0.78); C571(1.28); C575(1.28); C349(2.83)  LDD0591  [5]
 LDCM0621  AC154 HCT 116 C349(1.08); C571(1.05); C575(1.05); C587(0.94)  LDD2158  [5]
 LDCM0622  AC155 HCT 116 C349(1.23); C571(0.92); C575(0.92); C587(0.95)  LDD2159  [5]
 LDCM0623  AC156 HCT 116 C349(1.07); C571(0.86); C575(0.86); C587(0.79)  LDD2160  [5]
 LDCM0624  AC157 HCT 116 C349(0.99); C571(1.02); C575(1.02); C587(0.39)  LDD2161  [5]
 LDCM0276  AC17 HCT 116 C466(0.94)  LDD0593  [5]
 LDCM0277  AC18 HCT 116 C466(0.86)  LDD0594  [5]
 LDCM0278  AC19 HCT 116 C466(0.94)  LDD0595  [5]
 LDCM0279  AC2 HCT 116 C466(0.85); C208(0.97); C571(1.10); C575(1.10)  LDD0596  [5]
 LDCM0280  AC20 HCT 116 C466(0.88)  LDD0597  [5]
 LDCM0281  AC21 HCT 116 C466(0.78)  LDD0598  [5]
 LDCM0282  AC22 HCT 116 C466(0.92)  LDD0599  [5]
 LDCM0283  AC23 HCT 116 C466(0.92)  LDD0600  [5]
 LDCM0284  AC24 HCT 116 C466(0.82)  LDD0601  [5]
 LDCM0285  AC25 HCT 116 C349(0.95); C571(1.15); C575(1.15)  LDD0602  [5]
 LDCM0286  AC26 HCT 116 C571(1.04); C575(1.04); C349(1.24)  LDD0603  [5]
 LDCM0287  AC27 HCT 116 C571(1.06); C575(1.06); C349(1.23)  LDD0604  [5]
 LDCM0288  AC28 HCT 116 C571(1.09); C575(1.09); C349(1.21)  LDD0605  [5]
 LDCM0289  AC29 HCT 116 C571(0.93); C575(0.93); C349(1.43)  LDD0606  [5]
 LDCM0290  AC3 HCT 116 C208(0.84); C571(0.90); C575(0.90); C466(0.96)  LDD0607  [5]
 LDCM0291  AC30 HCT 116 C571(0.84); C575(0.84); C349(1.52)  LDD0608  [5]
 LDCM0292  AC31 HCT 116 C571(0.78); C575(0.78); C349(1.14)  LDD0609  [5]
 LDCM0293  AC32 HCT 116 C571(0.96); C575(0.96); C349(1.62)  LDD0610  [5]
 LDCM0294  AC33 HCT 116 C571(0.76); C575(0.76); C349(1.06)  LDD0611  [5]
 LDCM0295  AC34 HCT 116 C571(0.92); C575(0.92); C349(1.01)  LDD0612  [5]
 LDCM0296  AC35 HCT 116 C349(0.87); C208(0.96); C587(1.24)  LDD0613  [5]
 LDCM0297  AC36 HCT 116 C587(0.88); C208(0.94); C349(0.96)  LDD0614  [5]
 LDCM0298  AC37 HCT 116 C349(0.82); C208(0.86); C587(1.21)  LDD0615  [5]
 LDCM0299  AC38 HCT 116 C587(0.97); C349(0.99); C208(1.07)  LDD0616  [5]
 LDCM0300  AC39 HCT 116 C587(0.94); C349(1.08); C208(1.15)  LDD0617  [5]
 LDCM0301  AC4 HCT 116 C571(0.77); C575(0.77); C466(0.97); C208(1.28)  LDD0618  [5]
 LDCM0302  AC40 HCT 116 C587(1.11); C349(1.13); C208(1.16)  LDD0619  [5]
 LDCM0303  AC41 HCT 116 C587(0.93); C208(1.04); C349(1.10)  LDD0620  [5]
 LDCM0304  AC42 HCT 116 C587(0.89); C208(1.03); C349(1.16)  LDD0621  [5]
 LDCM0305  AC43 HCT 116 C587(0.80); C208(1.17); C349(1.22)  LDD0622  [5]
 LDCM0306  AC44 HCT 116 C349(0.93); C208(1.09); C587(1.53)  LDD0623  [5]
 LDCM0307  AC45 HCT 116 C349(0.98); C208(1.00); C587(1.37)  LDD0624  [5]
 LDCM0308  AC46 HCT 116 C208(0.89); C349(0.95); C466(0.97); C571(1.01)  LDD0625  [5]
 LDCM0309  AC47 HCT 116 C208(0.72); C571(1.09); C575(1.09); C466(1.11)  LDD0626  [5]
 LDCM0310  AC48 HCT 116 C208(0.85); C466(1.05); C349(1.22); C571(1.41)  LDD0627  [5]
 LDCM0311  AC49 HCT 116 C208(0.86); C571(0.96); C575(0.96); C466(1.08)  LDD0628  [5]
 LDCM0312  AC5 HCT 116 C571(0.68); C575(0.68); C466(1.05); C208(1.11)  LDD0629  [5]
 LDCM0313  AC50 HCT 116 C571(0.96); C575(0.96); C466(1.02); C208(1.07)  LDD0630  [5]
 LDCM0314  AC51 HCT 116 C208(0.73); C349(0.79); C466(0.93); C571(1.04)  LDD0631  [5]
 LDCM0315  AC52 HCT 116 C208(0.83); C466(0.91); C571(1.04); C575(1.04)  LDD0632  [5]
 LDCM0316  AC53 HCT 116 C208(0.94); C466(0.95); C571(1.07); C575(1.07)  LDD0633  [5]
 LDCM0317  AC54 HCT 116 C208(0.98); C466(1.01); C571(1.24); C575(1.24)  LDD0634  [5]
 LDCM0318  AC55 HCT 116 C571(0.96); C575(0.96); C208(0.98); C466(1.05)  LDD0635  [5]
 LDCM0319  AC56 HCT 116 C466(0.84); C208(1.10); C571(1.52); C575(1.52)  LDD0636  [5]
 LDCM0320  AC57 HCT 116 C571(0.56); C575(0.56); C466(0.63); C390(0.92)  LDD0637  [5]
 LDCM0321  AC58 HCT 116 C571(0.41); C575(0.41); C466(0.63); C390(1.10)  LDD0638  [5]
 LDCM0322  AC59 HCT 116 C571(0.62); C575(0.62); C466(0.75); C390(1.01)  LDD0639  [5]
 LDCM0323  AC6 HCT 116 C571(0.96); C575(0.96); C349(1.45); C587(1.74)  LDD0640  [5]
 LDCM0324  AC60 HCT 116 C571(0.49); C575(0.49); C466(0.74); C390(0.98)  LDD0641  [5]
 LDCM0325  AC61 HCT 116 C571(0.52); C575(0.52); C466(0.62); C390(0.95)  LDD0642  [5]
 LDCM0326  AC62 HCT 116 C571(0.68); C575(0.68); C466(0.78); C390(1.02)  LDD0643  [5]
 LDCM0327  AC63 HCT 116 C571(0.44); C575(0.44); C466(0.54); C390(0.92)  LDD0644  [5]
 LDCM0328  AC64 HCT 116 C571(0.72); C575(0.72); C466(0.74); C390(0.89)  LDD0645  [5]
 LDCM0329  AC65 HCT 116 C571(0.58); C575(0.58); C390(1.07); C466(1.13)  LDD0646  [5]
 LDCM0330  AC66 HCT 116 C571(0.59); C575(0.59); C466(0.87); C390(1.12)  LDD0647  [5]
 LDCM0331  AC67 HCT 116 C571(0.56); C575(0.56); C466(0.84); C390(0.87)  LDD0648  [5]
 LDCM0332  AC68 HCT 116 C587(0.88); C571(0.95); C575(0.95)  LDD0649  [5]
 LDCM0333  AC69 HCT 116 C587(0.82); C571(1.11); C575(1.11)  LDD0650  [5]
 LDCM0334  AC7 HCT 116 C571(0.81); C575(0.81); C349(1.21); C587(1.69)  LDD0651  [5]
 LDCM0335  AC70 HCT 116 C571(0.76); C575(0.76); C587(0.95)  LDD0652  [5]
 LDCM0336  AC71 HCT 116 C571(1.03); C575(1.03); C587(1.04)  LDD0653  [5]
 LDCM0337  AC72 HCT 116 C587(0.88); C571(0.91); C575(0.91)  LDD0654  [5]
 LDCM0338  AC73 HCT 116 C571(0.81); C575(0.81); C587(0.98)  LDD0655  [5]
 LDCM0339  AC74 HCT 116 C587(0.85); C571(0.94); C575(0.94)  LDD0656  [5]
 LDCM0340  AC75 HCT 116 C571(0.91); C575(0.91); C587(0.99)  LDD0657  [5]
 LDCM0341  AC76 HCT 116 C571(0.91); C575(0.91); C587(1.07)  LDD0658  [5]
 LDCM0342  AC77 HCT 116 C571(0.89); C575(0.89); C587(1.07)  LDD0659  [5]
 LDCM0343  AC78 HCT 116 C571(0.77); C575(0.77); C587(0.88)  LDD0660  [5]
 LDCM0344  AC79 HCT 116 C587(0.97); C571(1.04); C575(1.04)  LDD0661  [5]
 LDCM0345  AC8 HCT 116 C571(0.80); C575(0.80); C349(1.30); C587(1.86)  LDD0662  [5]
 LDCM0346  AC80 HCT 116 C587(0.89); C571(0.94); C575(0.94)  LDD0663  [5]
 LDCM0347  AC81 HCT 116 C587(0.78); C571(1.16); C575(1.16)  LDD0664  [5]
 LDCM0348  AC82 HCT 116 C571(0.97); C575(0.97); C587(1.09)  LDD0665  [5]
 LDCM0349  AC83 HCT 116 C571(0.83); C575(0.83); C349(1.23)  LDD0666  [5]
 LDCM0350  AC84 HCT 116 C571(0.98); C575(0.98); C349(1.10)  LDD0667  [5]
 LDCM0351  AC85 HCT 116 C571(1.02); C575(1.02); C349(1.18)  LDD0668  [5]
 LDCM0352  AC86 HCT 116 C571(0.91); C575(0.91); C349(1.32)  LDD0669  [5]
 LDCM0353  AC87 HCT 116 C571(0.92); C575(0.92); C349(1.25)  LDD0670  [5]
 LDCM0354  AC88 HCT 116 C571(0.95); C575(0.95); C349(1.25)  LDD0671  [5]
 LDCM0355  AC89 HCT 116 C571(1.00); C575(1.00); C349(1.38)  LDD0672  [5]
 LDCM0357  AC90 HCT 116 C571(0.97); C575(0.97); C349(1.36)  LDD0674  [5]
 LDCM0358  AC91 HCT 116 C349(0.99); C571(1.05); C575(1.05)  LDD0675  [5]
 LDCM0359  AC92 HCT 116 C571(1.01); C575(1.01); C349(1.09)  LDD0676  [5]
 LDCM0360  AC93 HCT 116 C571(0.89); C575(0.89); C349(1.08)  LDD0677  [5]
 LDCM0361  AC94 HCT 116 C349(0.81); C571(1.38); C575(1.38)  LDD0678  [5]
 LDCM0362  AC95 HCT 116 C571(1.13); C575(1.13); C349(1.39)  LDD0679  [5]
 LDCM0363  AC96 HCT 116 C349(0.79); C571(1.30); C575(1.30)  LDD0680  [5]
 LDCM0364  AC97 HCT 116 C571(1.22); C575(1.22); C349(1.47)  LDD0681  [5]
 LDCM0365  AC98 HCT 116 C571(0.65); C575(0.65); C349(1.07)  LDD0682  [5]
 LDCM0366  AC99 HCT 116 C571(0.77); C575(0.77); C349(1.04)  LDD0683  [5]
 LDCM0248  AKOS034007472 HCT 116 C349(1.18); C571(0.80); C575(0.80); C587(1.76)  LDD0565  [5]
 LDCM0356  AKOS034007680 HCT 116 C571(0.75); C575(0.75); C349(1.22); C587(1.95)  LDD0673  [5]
 LDCM0275  AKOS034007705 HCT 116 C571(0.87); C575(0.87); C349(2.22); C587(2.37)  LDD0592  [5]
 LDCM0020  ARS-1620 HCC44 C349(0.95)  LDD2171  [5]
 LDCM0200  BPK-25 T cell C349(4.14)  LDD0516  [6]
 LDCM0367  CL1 HCT 116 C208(0.73); C390(0.85); C587(0.96); C571(1.30)  LDD0684  [5]
 LDCM0368  CL10 HCT 116 C208(0.69); C571(0.77); C575(0.77); C390(0.81)  LDD0685  [5]
 LDCM0369  CL100 HCT 116 C571(0.83); C575(0.83); C466(0.88); C208(0.95)  LDD0686  [5]
 LDCM0370  CL101 HCT 116 C571(1.26); C575(1.26); C349(1.51); C587(2.17)  LDD0687  [5]
 LDCM0371  CL102 HCT 116 C349(1.07); C571(1.13); C575(1.13); C587(1.43)  LDD0688  [5]
 LDCM0372  CL103 HCT 116 C349(1.05); C571(1.09); C575(1.09); C587(1.32)  LDD0689  [5]
 LDCM0373  CL104 HCT 116 C587(1.06); C571(1.12); C575(1.12); C349(1.16)  LDD0690  [5]
 LDCM0374  CL105 HCT 116 C466(0.91)  LDD0691  [5]
 LDCM0375  CL106 HCT 116 C466(0.98)  LDD0692  [5]
 LDCM0376  CL107 HCT 116 C466(0.95)  LDD0693  [5]
 LDCM0377  CL108 HCT 116 C466(0.96)  LDD0694  [5]
 LDCM0378  CL109 HCT 116 C466(0.95)  LDD0695  [5]
 LDCM0379  CL11 HCT 116 C390(0.68); C208(0.70); C571(0.74); C575(0.74)  LDD0696  [5]
 LDCM0380  CL110 HCT 116 C466(1.02)  LDD0697  [5]
 LDCM0381  CL111 HCT 116 C466(0.98)  LDD0698  [5]
 LDCM0382  CL112 HCT 116 C571(1.02); C575(1.02); C349(1.28)  LDD0699  [5]
 LDCM0383  CL113 HCT 116 C571(0.84); C575(0.84); C349(1.35)  LDD0700  [5]
 LDCM0384  CL114 HCT 116 C571(0.80); C575(0.80); C349(1.65)  LDD0701  [5]
 LDCM0385  CL115 HCT 116 C571(0.91); C575(0.91); C349(1.37)  LDD0702  [5]
 LDCM0386  CL116 HCT 116 C349(1.02); C571(1.17); C575(1.17)  LDD0703  [5]
 LDCM0387  CL117 HCT 116 C587(0.88); C208(1.24); C349(1.57)  LDD0704  [5]
 LDCM0388  CL118 HCT 116 C587(0.81); C208(1.03); C349(1.14)  LDD0705  [5]
 LDCM0389  CL119 HCT 116 C587(0.76); C208(1.03); C349(1.18)  LDD0706  [5]
 LDCM0390  CL12 HCT 116 C208(0.71); C571(0.72); C575(0.72); C390(0.85)  LDD0707  [5]
 LDCM0391  CL120 HCT 116 C587(0.89); C208(1.11); C349(1.18)  LDD0708  [5]
 LDCM0392  CL121 HCT 116 C349(0.49); C208(0.57); C466(0.92); C571(1.00)  LDD0709  [5]
 LDCM0393  CL122 HCT 116 C208(0.69); C466(0.92); C571(0.97); C575(0.97)  LDD0710  [5]
 LDCM0394  CL123 HCT 116 C466(0.68); C208(0.98); C571(1.42); C575(1.42)  LDD0711  [5]
 LDCM0395  CL124 HCT 116 C466(0.87); C208(0.94); C571(1.33); C575(1.33)  LDD0712  [5]
 LDCM0396  CL125 HCT 116 C466(0.81); C571(0.89); C575(0.89); C390(1.25)  LDD0713  [5]
 LDCM0397  CL126 HCT 116 C466(0.68); C571(0.76); C575(0.76); C390(1.30)  LDD0714  [5]
 LDCM0398  CL127 HCT 116 C466(0.84); C571(0.97); C575(0.97); C390(1.00)  LDD0715  [5]
 LDCM0399  CL128 HCT 116 C571(0.66); C575(0.66); C466(0.75); C390(1.11)  LDD0716  [5]
 LDCM0400  CL13 HCT 116 C390(0.71); C571(0.72); C575(0.72); C208(0.89)  LDD0717  [5]
 LDCM0401  CL14 HCT 116 C390(0.80); C571(0.83); C575(0.83); C208(0.90)  LDD0718  [5]
 LDCM0402  CL15 HCT 116 C390(0.67); C571(0.75); C575(0.75); C587(0.86)  LDD0719  [5]
 LDCM0403  CL16 HCT 116 C571(0.86); C575(0.86); C390(1.20)  LDD0720  [5]
 LDCM0404  CL17 HCT 116 C390(0.88); C571(0.99); C575(0.99)  LDD0721  [5]
 LDCM0405  CL18 HCT 116 C390(0.85); C571(0.87); C575(0.87)  LDD0722  [5]
 LDCM0406  CL19 HCT 116 C571(0.86); C575(0.86); C390(1.06)  LDD0723  [5]
 LDCM0407  CL2 HCT 116 C208(0.61); C587(0.93); C390(1.01); C571(1.25)  LDD0724  [5]
 LDCM0408  CL20 HCT 116 C571(0.89); C575(0.89); C390(1.00)  LDD0725  [5]
 LDCM0409  CL21 HCT 116 C571(0.88); C575(0.88); C390(1.09)  LDD0726  [5]
 LDCM0410  CL22 HCT 116 C571(0.86); C575(0.86); C390(1.49)  LDD0727  [5]
 LDCM0411  CL23 HCT 116 C571(0.83); C575(0.83); C390(1.18)  LDD0728  [5]
 LDCM0412  CL24 HCT 116 C571(0.89); C575(0.89); C390(1.24)  LDD0729  [5]
 LDCM0413  CL25 HCT 116 C390(1.28); C571(1.09); C575(1.09)  LDD0730  [5]
 LDCM0414  CL26 HCT 116 C390(0.99); C571(0.87); C575(0.87)  LDD0731  [5]
 LDCM0415  CL27 HCT 116 C390(1.26); C571(1.00); C575(1.00)  LDD0732  [5]
 LDCM0416  CL28 HCT 116 C390(0.87); C571(0.90); C575(0.90)  LDD0733  [5]
 LDCM0417  CL29 HCT 116 C390(1.08); C571(0.86); C575(0.86)  LDD0734  [5]
 LDCM0418  CL3 HCT 116 C208(0.65); C390(0.76); C571(1.02); C575(1.02)  LDD0735  [5]
 LDCM0419  CL30 HCT 116 C390(1.03); C571(1.03); C575(1.03)  LDD0736  [5]
 LDCM0420  CL31 HCT 116 C349(1.06); C571(0.67); C575(0.67); C587(0.88)  LDD0737  [5]
 LDCM0421  CL32 HCT 116 C349(0.99); C571(0.45); C575(0.45); C587(0.83)  LDD0738  [5]
 LDCM0422  CL33 HCT 116 C349(1.13); C571(0.57); C575(0.57); C587(0.64)  LDD0739  [5]
 LDCM0423  CL34 HCT 116 C349(1.23); C571(0.57); C575(0.57); C587(0.80)  LDD0740  [5]
 LDCM0424  CL35 HCT 116 C349(1.15); C571(0.61); C575(0.61); C587(0.58)  LDD0741  [5]
 LDCM0425  CL36 HCT 116 C349(1.45); C571(0.65); C575(0.65); C587(0.89)  LDD0742  [5]
 LDCM0426  CL37 HCT 116 C349(1.03); C571(0.54); C575(0.54); C587(0.76)  LDD0743  [5]
 LDCM0428  CL39 HCT 116 C349(1.00); C571(0.58); C575(0.58); C587(0.74)  LDD0745  [5]
 LDCM0429  CL4 HCT 116 C208(0.67); C390(0.92); C571(1.05); C575(1.05)  LDD0746  [5]
 LDCM0430  CL40 HCT 116 C349(1.84); C571(0.56); C575(0.56); C587(0.77)  LDD0747  [5]
 LDCM0431  CL41 HCT 116 C349(1.10); C571(0.57); C575(0.57); C587(0.98)  LDD0748  [5]
 LDCM0432  CL42 HCT 116 C349(1.10); C571(0.60); C575(0.60); C587(0.26)  LDD0749  [5]
 LDCM0433  CL43 HCT 116 C349(1.13); C571(0.54); C575(0.54); C587(0.41)  LDD0750  [5]
 LDCM0434  CL44 HCT 116 C349(1.09); C571(0.51); C575(0.51); C587(0.75)  LDD0751  [5]
 LDCM0435  CL45 HCT 116 C349(1.47); C571(0.58); C575(0.58); C587(0.68)  LDD0752  [5]
 LDCM0436  CL46 HCT 116 C349(0.67); C466(1.19)  LDD0753  [5]
 LDCM0437  CL47 HCT 116 C349(0.75); C466(1.14)  LDD0754  [5]
 LDCM0438  CL48 HCT 116 C349(0.82); C466(1.20)  LDD0755  [5]
 LDCM0439  CL49 HCT 116 C349(0.80); C466(1.31)  LDD0756  [5]
 LDCM0440  CL5 HCT 116 C208(1.11); C390(0.88); C571(0.86); C575(0.86)  LDD0757  [5]
 LDCM0441  CL50 HCT 116 C349(0.96); C466(1.11)  LDD0758  [5]
 LDCM0442  CL51 HCT 116 C349(0.84); C466(1.31)  LDD0759  [5]
 LDCM0443  CL52 HCT 116 C349(0.75); C466(1.30)  LDD0760  [5]
 LDCM0444  CL53 HCT 116 C349(1.15); C466(0.78)  LDD0761  [5]
 LDCM0445  CL54 HCT 116 C349(0.70); C466(1.14)  LDD0762  [5]
 LDCM0446  CL55 HCT 116 C349(0.76); C466(0.87)  LDD0763  [5]
 LDCM0447  CL56 HCT 116 C349(0.96); C466(0.92)  LDD0764  [5]
 LDCM0448  CL57 HCT 116 C349(0.89); C466(0.82)  LDD0765  [5]
 LDCM0449  CL58 HCT 116 C349(0.86); C466(1.12)  LDD0766  [5]
 LDCM0450  CL59 HCT 116 C349(1.10); C466(0.96)  LDD0767  [5]
 LDCM0451  CL6 HCT 116 C208(1.22); C390(0.84); C571(0.69); C575(0.69)  LDD0768  [5]
 LDCM0452  CL60 HCT 116 C349(0.89); C466(1.26)  LDD0769  [5]
 LDCM0453  CL61 PaTu 8988t C571(0.77); C575(0.77); C466(1.10); C587(0.78)  LDD1332  [5]
 LDCM0454  CL62 PaTu 8988t C571(0.70); C575(0.70); C466(0.74); C587(0.48)  LDD1333  [5]
 LDCM0455  CL63 PaTu 8988t C571(0.80); C575(0.80); C466(1.01); C587(0.50)  LDD1334  [5]
 LDCM0456  CL64 PaTu 8988t C571(0.85); C575(0.85); C466(0.90); C587(0.67)  LDD1335  [5]
 LDCM0457  CL65 PaTu 8988t C571(0.72); C575(0.72); C466(0.89); C587(0.63)  LDD1336  [5]
 LDCM0458  CL66 PaTu 8988t C571(0.71); C575(0.71); C466(0.93); C587(0.75)  LDD1337  [5]
 LDCM0459  CL67 PaTu 8988t C571(0.72); C575(0.72); C466(1.15); C587(0.64)  LDD1338  [5]
 LDCM0460  CL68 PaTu 8988t C571(0.66); C575(0.66); C466(0.92); C587(0.92)  LDD1339  [5]
 LDCM0461  CL69 PaTu 8988t C571(0.76); C575(0.76); C466(0.98); C587(0.92)  LDD1340  [5]
 LDCM0462  CL7 HCT 116 C208(1.06); C390(0.85); C571(0.73); C575(0.73)  LDD0779  [5]
 LDCM0463  CL70 PaTu 8988t C571(0.69); C575(0.69); C466(1.05); C587(0.78)  LDD1342  [5]
 LDCM0464  CL71 PaTu 8988t C571(0.82); C575(0.82); C466(0.92); C587(0.52)  LDD1343  [5]
 LDCM0465  CL72 PaTu 8988t C571(0.98); C575(0.98); C466(1.00); C587(0.72)  LDD1344  [5]
 LDCM0466  CL73 PaTu 8988t C571(0.57); C575(0.57); C466(0.75); C587(0.82)  LDD1345  [5]
 LDCM0467  CL74 PaTu 8988t C571(0.59); C575(0.59); C466(0.99); C587(0.69)  LDD1346  [5]
 LDCM0469  CL76 HCT 116 C349(1.32); C390(0.96); C466(0.81); C587(1.22)  LDD0786  [5]
 LDCM0470  CL77 HCT 116 C349(1.28); C390(0.91); C466(0.97); C587(0.98)  LDD0787  [5]
 LDCM0471  CL78 HCT 116 C349(1.27); C390(0.87); C466(0.93); C587(1.23)  LDD0788  [5]
 LDCM0472  CL79 HCT 116 C349(1.83); C390(1.01); C466(0.81); C587(1.44)  LDD0789  [5]
 LDCM0473  CL8 HCT 116 C208(0.92); C390(0.83); C571(0.62); C575(0.62)  LDD0790  [5]
 LDCM0474  CL80 HCT 116 C349(1.26); C390(0.99); C466(0.55); C587(1.35)  LDD0791  [5]
 LDCM0475  CL81 HCT 116 C349(1.56); C390(0.89); C466(0.78); C587(0.95)  LDD0792  [5]
 LDCM0476  CL82 HCT 116 C349(2.56); C390(0.93); C466(0.81); C587(1.38)  LDD0793  [5]
 LDCM0477  CL83 HCT 116 C349(2.43); C390(0.73); C466(0.80); C587(1.25)  LDD0794  [5]
 LDCM0478  CL84 HCT 116 C349(3.52); C390(0.82); C466(0.96); C587(1.29)  LDD0795  [5]
 LDCM0479  CL85 HCT 116 C349(1.54); C390(0.75); C466(0.84); C587(1.12)  LDD0796  [5]
 LDCM0480  CL86 HCT 116 C349(1.33); C390(0.68); C466(0.89); C587(1.82)  LDD0797  [5]
 LDCM0481  CL87 HCT 116 C349(1.75); C390(0.62); C466(1.07); C587(1.31)  LDD0798  [5]
 LDCM0482  CL88 HCT 116 C349(2.17); C390(0.72); C466(0.86); C587(1.48)  LDD0799  [5]
 LDCM0483  CL89 HCT 116 C349(3.68); C390(0.77); C466(0.98); C587(1.21)  LDD0800  [5]
 LDCM0484  CL9 HCT 116 C208(1.12); C390(0.65); C571(0.76); C575(0.76)  LDD0801  [5]
 LDCM0485  CL90 HCT 116 C349(1.33); C390(0.70); C466(0.96); C587(0.95)  LDD0802  [5]
 LDCM0486  CL91 HCT 116 C208(1.03); C349(1.34); C466(1.00); C571(1.26)  LDD0803  [5]
 LDCM0487  CL92 HCT 116 C208(0.86); C349(1.33); C466(0.79); C571(1.50)  LDD0804  [5]
 LDCM0488  CL93 HCT 116 C208(1.07); C349(1.13); C466(0.85); C571(1.03)  LDD0805  [5]
 LDCM0489  CL94 HCT 116 C208(1.12); C349(1.63); C466(0.98); C571(1.19)  LDD0806  [5]
 LDCM0490  CL95 HCT 116 C208(0.91); C349(1.73); C466(0.83); C571(0.93)  LDD0807  [5]
 LDCM0491  CL96 HCT 116 C208(1.21); C349(1.35); C466(0.86); C571(0.99)  LDD0808  [5]
 LDCM0492  CL97 HCT 116 C208(1.38); C349(1.43); C466(0.72); C571(1.11)  LDD0809  [5]
 LDCM0493  CL98 HCT 116 C208(0.95); C349(1.40); C466(0.95); C571(1.10)  LDD0810  [5]
 LDCM0494  CL99 HCT 116 C208(0.95); C349(1.28); C466(0.92); C571(1.09)  LDD0811  [5]
 LDCM0495  E2913 HEK-293T C466(0.94); C208(0.97); C349(1.34)  LDD1698  [7]
 LDCM0203  EV-96 T cell C349(6.57)  LDD0523  [6]
 LDCM0206  EV-99 T cell C349(4.46)  LDD0521  [6]
 LDCM0625  F8 Ramos C349(1.40); C208(1.46)  LDD2187  [8]
 LDCM0572  Fragment10 Ramos C208(0.61)  LDD2189  [8]
 LDCM0573  Fragment11 Ramos C349(3.57); C208(1.55)  LDD2190  [8]
 LDCM0575  Fragment13 Ramos C349(0.85); C208(0.82)  LDD2192  [8]
 LDCM0576  Fragment14 Ramos C349(1.55); C208(0.80)  LDD2193  [8]
 LDCM0579  Fragment20 Ramos C208(0.57)  LDD2194  [8]
 LDCM0580  Fragment21 Ramos C349(1.03); C390(1.82); C208(0.80)  LDD2195  [8]
 LDCM0582  Fragment23 Ramos C349(1.60); C390(1.01); C208(1.39)  LDD2196  [8]
 LDCM0578  Fragment27 Ramos C349(0.71); C208(0.85)  LDD2197  [8]
 LDCM0586  Fragment28 Ramos C349(0.79); C390(0.67); C208(0.63)  LDD2198  [8]
 LDCM0588  Fragment30 Ramos C349(1.46); C208(1.18)  LDD2199  [8]
 LDCM0589  Fragment31 Ramos C349(0.76); C208(0.85)  LDD2200  [8]
 LDCM0590  Fragment32 Ramos C208(0.69)  LDD2201  [8]
 LDCM0468  Fragment33 PaTu 8988t C571(0.80); C575(0.80); C466(1.06); C587(0.67)  LDD1347  [5]
 LDCM0596  Fragment38 Ramos C349(1.62); C390(3.30); C208(0.50)  LDD2203  [8]
 LDCM0566  Fragment4 Ramos C349(1.44)  LDD2184  [8]
 LDCM0427  Fragment51 HCT 116 C349(2.12); C571(0.58); C575(0.58); C587(0.35)  LDD0744  [5]
 LDCM0610  Fragment52 Ramos C349(0.82); C208(1.37)  LDD2204  [8]
 LDCM0614  Fragment56 Ramos C349(0.90); C208(0.96)  LDD2205  [8]
 LDCM0569  Fragment7 Ramos C208(1.04)  LDD2186  [8]
 LDCM0022  KB02 T cell C349(16.80)  LDD1703  [6]
 LDCM0023  KB03 Jurkat C349(21.61); C297(14.56)  LDD0209  [1]
 LDCM0024  KB05 HMCB C208(1.48)  LDD3312  [9]
 LDCM0021  THZ1 HCT 116 C349(0.95)  LDD2173  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Matrix metalloproteinase-14 (MMP14) Peptidase M10A family P50281
Serine/threonine-protein kinase LATS1 (LATS1) AGC Ser/Thr protein kinase family O95835
Serine/threonine-protein kinase PAK 1 (PAK1) STE Ser/Thr protein kinase family Q13153
Serine/threonine-protein kinase PAK 2 (PAK2) STE Ser/Thr protein kinase family Q13177
Protein phosphatase Slingshot homolog 1 (SSH1) Protein-tyrosine phosphatase family Q8WYL5
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cofilin-1 (CFL1) Actin-binding proteins ADF family P23528

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Dabrafenib Small molecular drug DB08912
Fostamatinib Small molecular drug DB12010
Investigative
Click To Hide/Show 5 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Pmid19831390c14 Small molecular drug D07XWC
Pmid22902653c30 Small molecular drug D0F6NZ
Pmid22902653c31 Small molecular drug D0FC4Y
Pmid22902653c35 Small molecular drug D0S0XD
Rki-1447 Small molecular drug D03CPB

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
9 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840