Details of the Target

General Information of Target

Target ID LDTP04420
Target Name DNA replication ATP-dependent helicase/nuclease DNA2 (DNA2)
Gene Name DNA2
Gene ID 1763
Synonyms
DNA2L; KIAA0083; DNA replication ATP-dependent helicase/nuclease DNA2; hDNA2; DNA replication ATP-dependent helicase-like homolog) [Includes: DNA replication nuclease DNA2; EC 3.1.-.-; DNA replication ATP-dependent helicase DNA2; EC 3.6.4.12)]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEQLNELELLMEKSFWEEAELPAELFQKKVVASFPRTVLSTGMDNRYLVLAVNTVQNKEG
NCEKRLVITASQSLENKELCILRNDWCSVPVEPGDIIHLEGDCTSDTWIIDKDFGYLILY
PDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAINNSFAPEKLQ
ELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSL
PSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELK
TGKESNSIEHRSQVVLYTLLSQERRADPEAGLLLYLKTGQMYPVPANHLDKRELLKLRNQ
MAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPI
VMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNHQNIWLMPASEMEKSGSCIG
NLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINM
TTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFRE
PQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICT
LVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRS
KSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLFFSR
RFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKL
TYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKV
PAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGM
VEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSL
NCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGDFQRE
Target Bioclass
Enzyme
Family
DNA2/NAM7 helicase family
Subcellular location
Nucleus
Function
Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
Uniprot ID
P51530
Ensemble ID
ENST00000358410.8
HGNC ID
HGNC:2939
ChEMBL ID
CHEMBL4523236

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.Y288H .
HCT15 SNV: p.P1025H .
KMCH1 SNV: p.F366C DBIA    Probe Info 
MDAMB436 SNV: p.H461R DBIA    Probe Info 
NCIH2172 SNV: p.V761L .
OVKATE SNV: p.S735N .
OVTOKO SNV: p.L9V DBIA    Probe Info 
SKMEL30 SNV: p.P749S .
SW403 SNV: p.A667S DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C402(3.82); C848(2.00); C621(1.77)  LDD0080  [1]
IA-alkyne
 Probe Info  		C478(0.00); C1016(0.00); C932(0.00); C569(0.00)  LDD0165  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
IPM
 Probe Info  		C569(0.00); C492(0.00); C621(0.00); C415(0.00)  LDD2156  [4]
TFBX
 Probe Info  		C718(0.00); C218(0.00)  LDD0148  [5]
AOyne
 Probe Info  		12.50  LDD0443  [6]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C091
 Probe Info  		10.41  LDD1782  [7]
C092
 Probe Info  		17.15  LDD1783  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C569(7.36)  LDD0371  [8]
 LDCM0214  AC1 HCT 116 C396(1.34); C402(1.34); C718(0.83)  LDD0531  [1]
 LDCM0215  AC10 HCT 116 C396(0.90); C402(0.94); C621(0.74)  LDD0532  [1]
 LDCM0216  AC100 HCT 116 C396(0.76); C402(0.76)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C396(0.92); C402(0.92)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C396(0.90); C402(0.90)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C396(0.97); C402(0.97)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C396(0.94); C402(0.94)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C396(0.79); C402(0.79)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C396(0.78); C402(0.78)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C396(0.91); C402(0.91)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C396(0.68); C402(0.68)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C396(0.74); C402(0.74)  LDD0542  [1]
 LDCM0226  AC11 HCT 116 C396(1.19); C402(1.23); C621(0.91)  LDD0543  [1]
 LDCM0227  AC110 HCT 116 C396(0.65); C402(0.65)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C396(0.63); C402(0.63)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C396(0.80); C402(0.80)  LDD0546  [1]
 LDCM0230  AC113 HCT 116 C396(0.78); C402(0.78)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C396(1.01); C402(0.94)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C396(1.01); C402(0.92)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C396(1.02); C402(0.96)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C396(1.12); C402(0.87)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C396(1.12); C402(0.96)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C396(1.13); C402(1.01)  LDD0553  [1]
 LDCM0237  AC12 HCT 116 C396(0.95); C402(1.07); C621(0.91)  LDD0554  [1]
 LDCM0238  AC120 HCT 116 C396(1.01); C402(1.01)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C396(0.92); C402(0.89)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C396(0.99); C402(1.00)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C396(1.06); C402(0.92)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C396(1.01); C402(0.95)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C396(1.00); C402(1.13)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C396(1.02); C402(1.10)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C396(1.10); C402(0.98)  LDD0562  [1]
 LDCM0246  AC128 HCT 116 C396(0.87); C402(0.87); C718(0.91)  LDD0563  [1]
 LDCM0247  AC129 HCT 116 C396(0.79); C402(0.79); C718(0.96)  LDD0564  [1]
 LDCM0249  AC130 HCT 116 C396(0.73); C402(0.73); C718(0.99)  LDD0566  [1]
 LDCM0250  AC131 HCT 116 C396(1.05); C402(1.05); C718(0.84)  LDD0567  [1]
 LDCM0251  AC132 HCT 116 C396(0.58); C402(0.58); C718(0.91)  LDD0568  [1]
 LDCM0252  AC133 HCT 116 C396(0.72); C402(0.72); C718(0.92)  LDD0569  [1]
 LDCM0253  AC134 HCT 116 C396(0.84); C402(0.84); C718(0.98)  LDD0570  [1]
 LDCM0254  AC135 HCT 116 C396(0.88); C402(0.88); C718(0.90)  LDD0571  [1]
 LDCM0255  AC136 HCT 116 C396(0.70); C402(0.70); C718(0.97)  LDD0572  [1]
 LDCM0256  AC137 HCT 116 C396(0.68); C402(0.68); C718(0.94)  LDD0573  [1]
 LDCM0257  AC138 HCT 116 C396(0.97); C402(0.97); C718(1.03)  LDD0574  [1]
 LDCM0258  AC139 HCT 116 C396(0.75); C402(0.75); C718(1.07)  LDD0575  [1]
 LDCM0259  AC14 HCT 116 C396(1.13); C402(1.13); C621(0.89)  LDD0576  [1]
 LDCM0260  AC140 HCT 116 C396(0.82); C402(0.82); C718(0.92)  LDD0577  [1]
 LDCM0261  AC141 HCT 116 C396(1.03); C402(1.03); C718(1.13)  LDD0578  [1]
 LDCM0262  AC142 HCT 116 C396(0.61); C402(0.61); C718(1.03)  LDD0579  [1]
 LDCM0263  AC143 HCT 116 C396(0.94); C402(0.94); C492(1.17); C502(1.17)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C492(1.21); C502(1.21); C396(1.29); C402(1.31)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C492(1.04); C502(1.04); C396(1.28); C402(1.31)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C402(1.16); C396(1.19); C492(1.26); C502(1.26)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C492(1.30); C502(1.30); C402(1.40); C396(1.41)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C402(1.45); C396(1.46); C492(1.77); C502(1.77)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C402(1.36); C396(1.39); C492(1.59); C502(1.59)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C621(0.85); C396(1.22); C402(1.26)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C492(0.82); C502(0.82); C402(1.00); C396(1.02)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C492(0.95); C502(0.95); C396(1.09); C402(1.09)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C492(1.30); C502(1.30); C402(1.33); C396(1.33)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C492(1.56); C502(1.56); C402(1.57); C396(1.59)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C396(1.38); C402(1.38); C492(1.31); C502(1.31)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C396(1.14); C402(1.15); C492(1.43); C502(1.43)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C396(1.22); C402(1.20); C492(1.23); C502(1.23)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C396(1.41); C402(1.42); C492(1.27); C502(1.27)  LDD2161  [1]
 LDCM0276  AC17 HCT 116 C492(0.94); C502(0.94); C396(1.14); C402(1.14)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C492(1.31); C502(1.31); C396(1.50); C402(1.50)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C492(1.19); C502(1.19); C396(1.54); C402(1.54)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C718(0.96); C396(1.06); C402(1.06)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C492(1.02); C502(1.02); C396(1.13); C402(1.13)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C492(0.97); C502(0.97); C396(1.71); C402(1.71)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C492(1.11); C502(1.11); C396(1.21); C402(1.21)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C492(1.02); C502(1.02); C396(1.22); C402(1.22)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C492(1.03); C502(1.03); C396(1.55); C402(1.55)  LDD0601  [1]
 LDCM0285  AC25 HCT 116 C718(0.73); C492(0.83); C502(0.83); C396(0.97)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C396(0.92); C402(0.92); C718(1.11); C492(1.26)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C718(1.06); C492(1.23); C502(1.23); C396(1.25)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C718(0.75); C396(0.97); C402(0.97); C492(1.23)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C396(0.98); C402(0.98); C718(1.16); C492(1.16)  LDD0606  [1]
 LDCM0290  AC3 HCT 116 C396(1.01); C402(1.01); C718(1.06)  LDD0607  [1]
 LDCM0291  AC30 HCT 116 C718(0.91); C396(1.11); C402(1.11); C492(1.40)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C718(0.86); C396(1.01); C402(1.01); C492(1.29)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C396(0.99); C402(0.99); C718(1.03); C492(1.49)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C718(0.94); C396(1.07); C402(1.07); C492(1.16)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C718(0.97); C492(1.04); C502(1.04); C396(2.16)  LDD0612  [1]
 LDCM0296  AC35 HCT 116 C621(0.79); C718(1.13); C396(1.22); C402(1.22)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C621(0.83); C396(0.95); C402(0.95); C718(1.24)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C621(0.69); C396(1.04); C402(1.04); C718(1.07)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C718(0.96); C396(1.07); C402(1.07); C621(1.08)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C718(0.88); C621(0.97); C396(1.18); C402(1.18)  LDD0617  [1]
 LDCM0301  AC4 HCT 116 C718(0.90); C396(1.63); C402(1.63)  LDD0618  [1]
 LDCM0302  AC40 HCT 116 C621(0.87); C718(0.90); C396(1.15); C402(1.15)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C621(0.82); C396(1.09); C402(1.09); C718(1.09)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C621(0.98); C718(1.12); C396(1.29); C402(1.29)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C621(1.00); C396(1.20); C402(1.20); C718(1.25)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C621(0.80); C718(0.96); C396(0.98); C402(0.98)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C621(0.69); C718(0.90); C396(1.18); C402(1.18)  LDD0624  [1]
 LDCM0308  AC46 HCT 116 C492(0.85); C502(0.85)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C492(0.93); C502(0.93)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C492(1.11); C502(1.11)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C492(1.31); C502(1.31)  LDD0628  [1]
 LDCM0312  AC5 HCT 116 C396(0.96); C402(0.96); C718(0.97)  LDD0629  [1]
 LDCM0313  AC50 HCT 116 C492(1.17); C502(1.17)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C492(0.71); C502(0.71)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C492(1.03); C502(1.03)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C492(1.11); C502(1.11)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C492(1.03); C502(1.03)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C492(1.08); C502(1.08)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C492(1.29); C502(1.29)  LDD0636  [1]
 LDCM0320  AC57 HCT 116 C396(0.64); C402(0.64); C492(0.76); C502(0.76)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C492(0.78); C502(0.78); C396(0.83); C402(0.83)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C396(0.68); C402(0.68); C492(0.78); C502(0.78)  LDD0639  [1]
 LDCM0323  AC6 HCT 116 C621(0.83); C402(0.96); C396(0.97)  LDD0640  [1]
 LDCM0324  AC60 HCT 116 C396(0.69); C402(0.69); C492(0.90); C502(0.90)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C396(0.46); C402(0.46); C492(1.03); C502(1.03)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C396(0.77); C402(0.77); C492(0.85); C502(0.85)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C396(0.72); C402(0.72); C492(0.94); C502(0.94)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C492(0.74); C502(0.74); C396(1.01); C402(1.01)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C396(0.41); C402(0.41); C492(0.97); C502(0.97)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C396(0.51); C402(0.51); C492(0.91); C502(0.91)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C396(0.45); C402(0.45); C492(0.93); C502(0.93)  LDD0648  [1]
 LDCM0332  AC68 HCT 116 C396(1.13); C402(1.13)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C396(1.39); C402(1.39)  LDD0650  [1]
 LDCM0334  AC7 HCT 116 C621(0.80); C396(0.88); C402(0.96)  LDD0651  [1]
 LDCM0335  AC70 HCT 116 C396(1.06); C402(1.06)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C396(1.09); C402(1.09)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C396(1.22); C402(1.22)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C396(1.53); C402(1.53)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C396(1.30); C402(1.30)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C396(1.38); C402(1.38)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C396(1.16); C402(1.16)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C396(1.47); C402(1.47)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C396(1.12); C402(1.12)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C396(1.35); C402(1.35)  LDD0661  [1]
 LDCM0345  AC8 HCT 116 C621(0.78); C396(1.08); C402(1.09)  LDD0662  [1]
 LDCM0346  AC80 HCT 116 C396(1.25); C402(1.25)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C396(1.24); C402(1.24)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C396(1.29); C402(1.29)  LDD0665  [1]
 LDCM0349  AC83 HCT 116 C396(1.22); C402(1.22); C492(1.55); C502(1.55)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C396(1.20); C402(1.20); C492(1.42); C502(1.42)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C492(1.02); C502(1.02); C396(1.04); C402(1.04)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C396(0.96); C402(0.96); C492(1.10); C502(1.10)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C396(0.88); C402(0.88); C492(1.03); C502(1.03)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C396(1.03); C402(1.03); C492(1.07); C502(1.07)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C492(1.17); C502(1.17); C396(1.25); C402(1.25)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C396(0.87); C402(0.87); C492(1.04); C502(1.04)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C396(1.25); C402(1.25); C492(1.30); C502(1.30)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C492(1.29); C502(1.29); C396(1.31); C402(1.31)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C396(1.14); C402(1.14); C492(1.23); C502(1.23)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C492(1.03); C502(1.03); C396(1.31); C402(1.31)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C492(0.92); C502(0.92); C396(1.03); C402(1.03)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C492(1.07); C502(1.07); C396(1.31); C402(1.31)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C396(1.26); C402(1.26); C492(1.28); C502(1.28)  LDD0681  [1]
 LDCM0365  AC98 HCT 116 C396(0.75); C402(0.75)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C396(0.71); C402(0.71)  LDD0683  [1]
 LDCM0248  AKOS034007472 HCT 116 C396(0.99); C402(1.00); C621(0.97)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C621(0.81); C396(0.89); C402(0.91)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C621(0.92); C396(1.30); C402(1.34)  LDD0592  [1]
 LDCM0020  ARS-1620 HCC44 C396(0.93); C402(0.93)  LDD2171  [1]
 LDCM0367  CL1 HEK-293T C396(1.15); C402(1.00); C492(1.36); C502(1.36)  LDD0965  [1]
 LDCM0368  CL10 HEK-293T C396(1.76); C402(2.09); C492(1.54); C502(1.54)  LDD0966  [1]
 LDCM0369  CL100 HCT 116 C396(1.04); C402(1.04); C718(1.06)  LDD0686  [1]
 LDCM0370  CL101 HCT 116 C621(0.78); C396(1.19); C402(1.30)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C621(0.77); C402(1.07); C396(1.08)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C621(0.79); C396(1.21); C402(1.23)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C621(0.84); C396(1.44); C402(1.46)  LDD0690  [1]
 LDCM0374  CL105 HCT 116 C492(1.27); C502(1.27); C396(1.28); C402(1.28)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C492(1.41); C502(1.41); C396(1.63); C402(1.63)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C396(1.26); C402(1.26); C492(1.30); C502(1.30)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C492(1.12); C502(1.12); C396(1.41); C402(1.41)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C492(1.47); C502(1.47); C396(1.62); C402(1.62)  LDD0695  [1]
 LDCM0379  CL11 HEK-293T C396(1.20); C402(1.87); C492(1.23); C502(1.23)  LDD0977  [1]
 LDCM0380  CL110 HCT 116 C492(1.32); C502(1.32); C396(1.88); C402(1.88)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C396(1.33); C402(1.33); C492(1.42); C502(1.42)  LDD0698  [1]
 LDCM0382  CL112 HCT 116 C718(0.81); C492(1.06); C502(1.06); C396(1.21)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C718(0.80); C396(1.31); C402(1.31); C492(1.43)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C718(0.80); C492(1.13); C502(1.13); C396(2.32)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C718(0.74); C492(1.06); C502(1.06); C396(1.45)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C718(0.78); C492(0.95); C502(0.95); C396(1.27)  LDD0703  [1]
 LDCM0387  CL117 HCT 116 C718(0.93); C621(1.06); C396(1.11); C402(1.11)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C718(0.93); C621(0.94); C396(1.02); C402(1.02)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C621(0.91); C718(0.95); C396(1.11); C402(1.11)  LDD0706  [1]
 LDCM0390  CL12 HEK-293T C396(0.84); C402(1.10); C492(1.09); C502(1.09)  LDD0988  [1]
 LDCM0391  CL120 HCT 116 C718(0.90); C621(1.02); C396(1.23); C402(1.23)  LDD0708  [1]
 LDCM0392  CL121 HCT 116 C492(1.42); C502(1.42)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C492(0.85); C502(0.85)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C492(1.73); C502(1.73)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C492(1.40); C502(1.40)  LDD0712  [1]
 LDCM0396  CL125 HCT 116 C396(0.48); C402(0.48); C492(0.82); C502(0.82)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C396(0.85); C402(0.85); C492(0.96); C502(0.96)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C396(0.65); C402(0.65); C492(0.86); C502(0.86)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C396(0.72); C402(0.72); C492(1.08); C502(1.08)  LDD0716  [1]
 LDCM0400  CL13 HEK-293T C396(0.86); C402(1.10); C492(1.21); C502(1.21)  LDD0998  [1]
 LDCM0401  CL14 HEK-293T C396(1.34); C402(1.10); C492(1.81); C502(1.81)  LDD0999  [1]
 LDCM0402  CL15 HEK-293T C396(2.94); C402(1.79); C492(1.82); C502(1.82)  LDD1000  [1]
 LDCM0403  CL16 HCT 116 C621(1.08)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C621(0.80)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C621(1.06)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C621(0.99)  LDD0723  [1]
 LDCM0407  CL2 HEK-293T C396(1.50); C402(1.23); C492(0.90); C502(0.90)  LDD1005  [1]
 LDCM0408  CL20 HCT 116 C621(0.97)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C621(0.92)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C621(0.84)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C621(1.21)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C621(1.02)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C621(0.87)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C621(1.09)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C621(0.96)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C621(0.98)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C621(1.12)  LDD0734  [1]
 LDCM0418  CL3 HEK-293T C396(0.91); C402(0.97); C492(0.77); C502(0.77)  LDD1016  [1]
 LDCM0419  CL30 HCT 116 C621(1.15)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C621(1.23); C718(0.84)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C621(1.04); C718(0.91)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C621(1.06); C718(0.96)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C621(1.02); C718(1.14)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C621(1.03); C718(1.04)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C621(1.07); C718(1.20)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C621(1.09); C718(1.00)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C621(0.98); C718(1.08)  LDD0745  [1]
 LDCM0429  CL4 HEK-293T C396(0.97); C402(1.08); C492(1.04); C502(1.04)  LDD1027  [1]
 LDCM0430  CL40 HCT 116 C621(1.24); C718(1.82)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C621(0.97); C718(0.92)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C621(0.94); C718(1.06)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C621(1.01); C718(1.06)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C621(0.97); C718(0.99)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C621(1.27); C718(1.53)  LDD0752  [1]
 LDCM0436  CL46 HCT 116 C396(0.82); C402(0.82); C492(1.28); C502(1.28)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C396(1.06); C402(1.06); C492(1.12); C502(1.12)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C396(0.82); C402(0.82); C492(1.29); C502(1.29)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C396(1.09); C402(1.09); C492(1.08); C502(1.08)  LDD0756  [1]
 LDCM0440  CL5 HEK-293T C396(0.95); C402(1.09); C492(1.27); C502(1.27)  LDD1038  [1]
 LDCM0441  CL50 HCT 116 C396(1.05); C402(1.05); C492(1.02); C502(1.02)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C396(1.05); C402(1.05); C492(1.14); C502(1.14)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C396(1.07); C402(1.07); C492(1.14); C502(1.14)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C396(1.50); C402(1.50); C492(1.11); C502(1.11)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C396(1.13); C402(1.13); C492(1.21); C502(1.21)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C396(1.15); C402(1.15); C492(1.15); C502(1.15)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C396(1.49); C402(1.49); C492(1.03); C502(1.03)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C396(1.28); C402(1.28); C492(1.07); C502(1.07)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C396(1.05); C402(1.05); C492(1.22); C502(1.22)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C396(0.97); C402(0.97); C492(1.03); C502(1.03)  LDD0767  [1]
 LDCM0451  CL6 HEK-293T C396(1.26); C402(1.16); C492(1.34); C502(1.34)  LDD1049  [1]
 LDCM0452  CL60 HCT 116 C396(1.07); C402(1.07); C492(1.05); C502(1.05)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C396(0.98); C402(0.98)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C396(0.85); C402(0.85)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C396(1.32); C402(1.32)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C396(0.92); C402(0.92)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C396(1.15); C402(1.15)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C396(1.11); C402(1.11)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C396(2.00); C402(2.00)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C396(1.78); C402(1.78)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C396(1.34); C402(1.34)  LDD0778  [1]
 LDCM0462  CL7 HEK-293T C396(0.80); C402(1.17); C492(1.07); C502(1.07)  LDD1060  [1]
 LDCM0463  CL70 HCT 116 C396(1.11); C402(1.11)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C396(1.17); C402(1.17)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C396(1.56); C402(1.56)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C396(1.79); C402(1.79)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C396(1.65); C402(1.65)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C396(0.91); C402(1.03); C492(0.95); C502(0.95)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C396(0.97); C402(0.82); C492(0.93); C502(0.93)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C396(1.11); C402(1.36); C492(1.12); C502(1.12)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C396(0.97); C402(0.94); C492(0.96); C502(0.96)  LDD0789  [1]
 LDCM0473  CL8 HEK-293T C396(1.03); C492(1.38); C502(1.87)  LDD1071  [1]
 LDCM0474  CL80 HCT 116 C396(0.97); C402(0.83); C492(1.08); C502(1.08)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C396(0.91); C402(0.72); C492(0.98); C502(0.98)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C396(0.96); C402(0.68); C492(1.01); C502(1.01)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C396(0.97); C402(0.96); C492(0.85); C502(0.85)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C396(1.03); C402(1.17); C492(0.97); C502(0.97)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C396(1.05); C402(0.89); C492(1.04); C502(1.04)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C396(1.11); C402(0.92); C492(1.04); C502(1.04)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C396(0.96); C402(0.94); C492(0.96); C502(0.96)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C396(1.07); C402(0.84); C492(0.90); C502(0.90)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C396(1.01); C402(0.86); C492(0.94); C502(0.94)  LDD0800  [1]
 LDCM0484  CL9 HEK-293T C396(1.34); C492(1.06); C502(1.27)  LDD1082  [1]
 LDCM0485  CL90 HCT 116 C396(1.66); C402(2.33); C492(1.71); C502(1.71)  LDD0802  [1]
 LDCM0486  CL91 HCT 116 C396(1.25); C402(1.25); C718(0.85)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C396(1.43); C402(1.43); C718(0.68)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C396(1.61); C402(1.61); C718(0.85)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C396(1.54); C402(1.54); C718(1.16)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C396(1.75); C402(1.75); C718(1.16)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C396(1.17); C402(1.17); C718(0.82)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C396(1.41); C402(1.41); C718(1.00)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C396(1.37); C402(1.37); C718(0.85)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C396(1.15); C402(1.15); C718(0.87)  LDD0811  [1]
 LDCM0495  E2913 HEK-293T C251(1.13)  LDD1698  [9]
 LDCM0468  Fragment33 HCT 116 C396(1.04); C402(1.04)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C621(1.44); C718(1.81)  LDD0744  [1]
 LDCM0022  KB02 HCT 116 C402(3.82); C848(2.00); C621(1.77)  LDD0080  [1]
 LDCM0023  KB03 HCT 116 C402(10.23); C848(1.27); C621(1.37)  LDD0081  [1]
 LDCM0024  KB05 HCT 116 C402(2.57); C848(1.55); C621(1.54)  LDD0082  [1]
 LDCM0021  THZ1 HCT 116 C396(0.93); C402(0.93)  LDD2173  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402