Details of the Target

General Information of Target

Target ID LDTP14073
Target Name Cyanocobalamin reductase / alkylcobalamin dealkylase (MMACHC)
Gene Name MMACHC
Gene ID 25974
Synonyms
Cyanocobalamin reductase / alkylcobalamin dealkylase; Alkylcobalamin:glutathione S-alkyltransferase; EC 2.5.1.151; CblC; Cyanocobalamin reductase; cyanide-eliminating); EC 1.16.1.6; Methylmalonic aciduria and homocystinuria type C protein; MMACHC
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEPAPSEVRLAVREAIHALSSSEDGGHIFCTLESLKRYLGEMEPPALPREKEEFASAHFS
PVLRCLASRLSPAWLELLPHGRLEELWASFFLEGPADQAFLVLMETIEGAAGPSFRLMKM
ARLLARFLREGRLAVLMEAQCRQQTQPGFILLRETLLGKVVALPDHLGNRLQQENLAEFF
PQNYFRLLGEEVVRVLQAVVDSLQGGLDSSVSFVSQVLGKACVHGRQQEILGVLVPRLAA
LTQGSYLHQRVCWRLVEQVPDRAMEAVLTGLVEAALGPEVLSRLLGNLVVKNKKAQFVMT
QKLLFLQSRLTTPMLQSLLGHLAMDSQRRPLLLQVLKELLETWGSSSAIRHTPLPQQRHV
SKAVLICLAQLGEPELRDSRDELLASMMAGVKCRLDSSLPPVRRLGMIVAEVVSARIHPE
GPPLKFQYEEDELSLELLALASPQPAGDGASEAGTSLVPATAEPPAETPAEIVDGGVPQA
QLAGSDSDLDSDDEFVPYDMSGDRELKSSKAPAYVRDCVEALTTSEDIERWEAALRALEG
LVYRSPTATREVSVELAKVLLHLEEKTCVVGFAGLRQRALVAVTVTDPAPVADYLTSQFY
ALNYSLRQRMDILDVLTLAAQELSRPGCLGRTPQPGSPSPNTPCLPEAAVSQPGSAVASD
WRVVVEERIRSKTQRLSKGGPRQGPAGSPSRFNSVAGHFFFPLLQRFDRPLVTFDLLGED
QLVLGRLAHTLGALMCLAVNTTVAVAMGKALLEFVWALRFHIDAYVRQGLLSAVSSVLLS
LPAARLLEDLMDELLEARSWLADVAEKDPDEDCRTLALRALLLLQRLKNRLLPPASP
Target Bioclass
Enzyme
Family
MMACHC family
Subcellular location
Cytoplasm, cytosol
Function
Cobalamin (vitamin B12) cytosolic chaperone that catalyzes the reductive decyanation of cyanocob(III)alamin (cyanocobalamin, CNCbl) to yield cob(II)alamin and cyanide, using FAD or FMN as cofactors and NADPH as cosubstrate. Cyanocobalamin constitutes the inactive form of vitamin B12 introduced from the diet, and is converted into the active cofactors methylcobalamin (MeCbl) involved in methionine biosynthesis, and 5'-deoxyadenosylcobalamin (AdoCbl) involved in the TCA cycle. Forms a complex with the lysosomal transporter ABCD4 and its chaperone LMBRD1, to transport cobalamin across the lysosomal membrane into the cytosol. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR (methionine synthase) which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine. Also acts as a glutathione transferase by catalyzing the dealkylation of the alkylcob(III)alamins MeCbl and AdoCbl, using the thiolate of glutathione for nucleophilic displacement to generate cob(I)alamin and the corresponding glutathione thioether. The conversion of incoming MeCbl or AdoCbl into a common intermediate cob(I)alamin is necessary to meet the cellular needs for both cofactors. Cysteine and homocysteine cannot substitute for glutathione in this reaction.
Uniprot ID
Q9Y4U1
Ensemble ID
ENST00000401061.9
HGNC ID
HGNC:24525

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0222  [1]
DBIA
 Probe Info  		C149(1.12)  LDD0531  [2]
m-APA
 Probe Info  		N.A.  LDD2233  [3]
IA-alkyne
 Probe Info  		C70(0.00); C149(0.00)  LDD0165  [4]
IPM
 Probe Info  		N.A.  LDD0147  [5]
TFBX
 Probe Info  		C70(0.00); C149(0.00)  LDD0148  [5]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [6]
NAIA_5
 Probe Info  		C70(0.00); C82(0.00)  LDD2223  [7]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C278
 Probe Info  		48.84  LDD1948  [8]
C280
 Probe Info  		9.25  LDD1950  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C149(1.12)  LDD0531  [2]
 LDCM0215  AC10 HCT 116 C149(0.99); C182(0.69)  LDD0532  [2]
 LDCM0216  AC100 HEK-293T C149(1.12)  LDD0814  [2]
 LDCM0217  AC101 HEK-293T C149(1.22)  LDD0815  [2]
 LDCM0218  AC102 HEK-293T C149(1.05)  LDD0816  [2]
 LDCM0219  AC103 HEK-293T C149(1.06)  LDD0817  [2]
 LDCM0220  AC104 HEK-293T C149(0.99)  LDD0818  [2]
 LDCM0221  AC105 HEK-293T C149(1.28)  LDD0819  [2]
 LDCM0222  AC106 HEK-293T C149(1.21)  LDD0820  [2]
 LDCM0223  AC107 HEK-293T C149(1.09)  LDD0821  [2]
 LDCM0224  AC108 HEK-293T C149(1.09)  LDD0822  [2]
 LDCM0225  AC109 HEK-293T C149(1.21)  LDD0823  [2]
 LDCM0226  AC11 HCT 116 C149(0.93); C182(0.73)  LDD0543  [2]
 LDCM0227  AC110 HEK-293T C149(1.22)  LDD0825  [2]
 LDCM0228  AC111 HEK-293T C149(1.09)  LDD0826  [2]
 LDCM0229  AC112 HEK-293T C149(1.19)  LDD0827  [2]
 LDCM0230  AC113 HCT 116 C149(0.89)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C149(0.82)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C149(0.80)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C149(0.83)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C149(0.82)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C149(0.92)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C149(0.95)  LDD0553  [2]
 LDCM0237  AC12 HCT 116 C149(1.17); C182(0.85)  LDD0554  [2]
 LDCM0238  AC120 HCT 116 C149(0.88)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C149(0.87)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C149(0.99)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C149(0.98)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C149(0.88)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C149(1.08)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C149(1.05)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C149(0.90)  LDD0562  [2]
 LDCM0246  AC128 HCT 116 C182(0.94)  LDD0563  [2]
 LDCM0247  AC129 HCT 116 C182(0.82)  LDD0564  [2]
 LDCM0249  AC130 HCT 116 C182(0.71)  LDD0566  [2]
 LDCM0250  AC131 HCT 116 C182(0.94)  LDD0567  [2]
 LDCM0251  AC132 HCT 116 C182(1.10)  LDD0568  [2]
 LDCM0252  AC133 HCT 116 C182(0.80)  LDD0569  [2]
 LDCM0253  AC134 HCT 116 C182(0.87)  LDD0570  [2]
 LDCM0254  AC135 HCT 116 C182(0.86)  LDD0571  [2]
 LDCM0255  AC136 HCT 116 C182(0.91)  LDD0572  [2]
 LDCM0256  AC137 HCT 116 C182(0.93)  LDD0573  [2]
 LDCM0257  AC138 HCT 116 C182(0.99)  LDD0574  [2]
 LDCM0258  AC139 HCT 116 C182(0.85)  LDD0575  [2]
 LDCM0259  AC14 HCT 116 C149(0.93); C182(0.50)  LDD0576  [2]
 LDCM0260  AC140 HCT 116 C182(0.96)  LDD0577  [2]
 LDCM0261  AC141 HCT 116 C182(0.95)  LDD0578  [2]
 LDCM0262  AC142 HCT 116 C182(0.85)  LDD0579  [2]
 LDCM0263  AC143 HEK-293T C149(1.16)  LDD0861  [2]
 LDCM0264  AC144 HEK-293T C149(1.08)  LDD0862  [2]
 LDCM0265  AC145 HEK-293T C149(0.87)  LDD0863  [2]
 LDCM0266  AC146 HEK-293T C149(0.89)  LDD0864  [2]
 LDCM0267  AC147 HEK-293T C149(1.65)  LDD0865  [2]
 LDCM0268  AC148 HEK-293T C149(0.98)  LDD0866  [2]
 LDCM0269  AC149 HEK-293T C149(1.26)  LDD0867  [2]
 LDCM0270  AC15 HCT 116 C182(0.69); C149(0.91)  LDD0587  [2]
 LDCM0271  AC150 HEK-293T C149(1.20)  LDD0869  [2]
 LDCM0272  AC151 HEK-293T C149(0.94)  LDD0870  [2]
 LDCM0273  AC152 HEK-293T C149(1.28)  LDD0871  [2]
 LDCM0274  AC153 HEK-293T C149(1.13)  LDD0872  [2]
 LDCM0621  AC154 HEK-293T C149(1.43)  LDD2162  [2]
 LDCM0622  AC155 HEK-293T C149(0.94)  LDD2163  [2]
 LDCM0623  AC156 HEK-293T C149(1.05)  LDD2164  [2]
 LDCM0624  AC157 HEK-293T C149(1.78)  LDD2165  [2]
 LDCM0276  AC17 HCT 116 C182(0.78); C149(0.99)  LDD0593  [2]
 LDCM0277  AC18 HCT 116 C182(0.66); C149(0.98)  LDD0594  [2]
 LDCM0278  AC19 HCT 116 C182(0.74); C149(1.06)  LDD0595  [2]
 LDCM0279  AC2 HCT 116 C149(1.17)  LDD0596  [2]
 LDCM0280  AC20 HCT 116 C182(0.77); C149(1.04)  LDD0597  [2]
 LDCM0281  AC21 HCT 116 C182(0.61); C149(0.94)  LDD0598  [2]
 LDCM0282  AC22 HCT 116 C149(0.92); C182(1.18)  LDD0599  [2]
 LDCM0283  AC23 HCT 116 C182(0.83); C149(0.95)  LDD0600  [2]
 LDCM0284  AC24 HCT 116 C149(0.91); C182(0.95)  LDD0601  [2]
 LDCM0285  AC25 HCT 116 C182(0.66); C149(1.05)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C182(0.58); C149(1.04)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C182(0.56); C149(1.05)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C182(0.61); C149(1.14)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C182(0.58); C149(1.13)  LDD0606  [2]
 LDCM0290  AC3 HCT 116 C149(1.22)  LDD0607  [2]
 LDCM0291  AC30 HCT 116 C182(0.56); C149(1.12)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C182(0.51); C149(1.05)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C182(0.50); C149(1.12)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C182(0.53); C149(1.01)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C182(0.47); C149(1.14)  LDD0612  [2]
 LDCM0296  AC35 HCT 116 C149(1.04); C182(1.17)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C149(0.93); C182(1.19)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C149(0.86); C182(1.01)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C182(0.99); C149(1.13)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C149(0.96); C182(1.08)  LDD0617  [2]
 LDCM0301  AC4 HCT 116 C149(1.24)  LDD0618  [2]
 LDCM0302  AC40 HCT 116 C149(0.97); C182(1.05)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C149(0.93); C182(1.03)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C149(0.99); C182(1.01)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C182(0.99); C149(0.99)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C149(0.84); C182(1.11)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C149(0.90); C182(1.01)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C149(0.92); C182(1.00)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C182(0.96); C149(1.01)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C149(0.91); C182(1.03)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C149(1.04); C182(1.07)  LDD0628  [2]
 LDCM0312  AC5 HCT 116 C149(1.28)  LDD0629  [2]
 LDCM0313  AC50 HCT 116 C149(0.91); C182(0.92)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C149(0.96); C182(1.01)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C149(0.93); C182(1.13)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C182(0.88); C149(0.93)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C182(0.79); C149(0.98)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C149(0.93); C182(0.98)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C149(0.98); C182(1.12)  LDD0636  [2]
 LDCM0320  AC57 HCT 116 C182(0.43); C149(0.59)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C182(0.46); C149(0.58)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C182(0.45); C149(0.73)  LDD0639  [2]
 LDCM0323  AC6 HCT 116 C182(0.64); C149(1.00)  LDD0640  [2]
 LDCM0324  AC60 HCT 116 C182(0.34); C149(0.55)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C182(0.23); C149(0.51)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C182(0.46); C149(0.55)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C182(0.30); C149(0.51)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C182(0.48); C149(0.58)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C182(0.49); C149(0.64)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C182(0.39); C149(0.58)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C182(0.39); C149(0.65)  LDD0648  [2]
 LDCM0332  AC68 HCT 116 C149(1.05)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C149(1.03)  LDD0650  [2]
 LDCM0334  AC7 HCT 116 C182(0.69); C149(0.92)  LDD0651  [2]
 LDCM0335  AC70 HCT 116 C149(1.02)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C149(1.11)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C149(1.03)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C149(1.07)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C149(1.08)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C149(1.00)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C149(1.11)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C149(0.93)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C149(1.04)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C149(1.10)  LDD0661  [2]
 LDCM0345  AC8 HCT 116 C182(0.82); C149(1.00)  LDD0662  [2]
 LDCM0346  AC80 HCT 116 C149(1.08)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C149(1.10)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C149(1.01)  LDD0665  [2]
 LDCM0349  AC83 HCT 116 C182(0.75)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C182(0.77)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C182(0.69)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C182(1.16)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C182(0.74)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C182(0.90)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C182(0.72)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C182(1.62)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C182(0.80)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C182(0.77)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C182(0.84)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C182(0.82)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C182(0.53)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C182(0.63)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C182(0.76)  LDD0681  [2]
 LDCM0365  AC98 HEK-293T C149(1.18)  LDD0963  [2]
 LDCM0366  AC99 HEK-293T C149(1.09)  LDD0964  [2]
 LDCM0248  AKOS034007472 HCT 116 C149(0.96); C182(1.02)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C182(0.87); C149(0.94)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C182(0.65); C149(1.06)  LDD0592  [2]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [1]
 LDCM0632  CL-Sc Hep-G2 C70(20.00)  LDD2227  [7]
 LDCM0367  CL1 HCT 116 C149(0.85)  LDD0684  [2]
 LDCM0368  CL10 HCT 116 C149(0.77)  LDD0685  [2]
 LDCM0369  CL100 HCT 116 C149(1.22)  LDD0686  [2]
 LDCM0370  CL101 HCT 116 C182(0.69); C149(0.97)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C182(0.81); C149(0.88)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C182(0.77); C149(1.14)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C182(0.80); C149(1.11)  LDD0690  [2]
 LDCM0374  CL105 HCT 116 C182(0.63); C149(0.92)  LDD0691  [2]
 LDCM0375  CL106 HCT 116 C182(0.55); C149(1.11)  LDD0692  [2]
 LDCM0376  CL107 HCT 116 C182(0.54); C149(0.92)  LDD0693  [2]
 LDCM0377  CL108 HCT 116 C182(0.60); C149(0.98)  LDD0694  [2]
 LDCM0378  CL109 HCT 116 C182(0.67); C149(0.96)  LDD0695  [2]
 LDCM0379  CL11 HCT 116 C149(0.78)  LDD0696  [2]
 LDCM0380  CL110 HCT 116 C182(0.60); C149(1.11)  LDD0697  [2]
 LDCM0381  CL111 HCT 116 C182(0.65); C149(0.99)  LDD0698  [2]
 LDCM0382  CL112 HCT 116 C182(0.77); C149(1.08)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C182(0.51); C149(1.01)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C182(0.62); C149(1.05)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C182(0.60); C149(1.00)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C182(0.62); C149(1.06)  LDD0703  [2]
 LDCM0387  CL117 HCT 116 C149(1.02); C182(1.15)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C149(1.05); C182(1.08)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C182(0.94); C149(0.99)  LDD0706  [2]
 LDCM0390  CL12 HCT 116 C149(0.79)  LDD0707  [2]
 LDCM0391  CL120 HCT 116 C149(0.89); C182(1.13)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C149(0.89); C182(1.16)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C182(0.83); C149(0.95)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C149(0.88); C182(0.94)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C149(0.98); C182(1.01)  LDD0712  [2]
 LDCM0396  CL125 HCT 116 C182(0.66); C149(0.77)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C182(0.38); C149(0.65)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C182(0.44); C149(0.78)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C182(0.45); C149(0.55)  LDD0716  [2]
 LDCM0400  CL13 HCT 116 C149(0.87)  LDD0717  [2]
 LDCM0401  CL14 HCT 116 C149(0.94)  LDD0718  [2]
 LDCM0402  CL15 HCT 116 C149(0.78)  LDD0719  [2]
 LDCM0403  CL16 HCT 116 C182(0.77); C149(0.85)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C182(0.63); C149(0.93)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C182(0.47); C149(1.01)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C182(0.54); C149(1.05)  LDD0723  [2]
 LDCM0407  CL2 HCT 116 C149(0.70)  LDD0724  [2]
 LDCM0408  CL20 HCT 116 C182(0.60); C149(0.86)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C182(0.62); C149(0.96)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C182(0.84); C149(0.87)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C182(0.61); C149(0.99)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C182(0.63); C149(1.00)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C149(1.20); C182(0.86)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C149(0.96); C182(0.64)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C149(0.92); C182(0.63)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C149(1.05); C182(0.63)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C149(0.81); C182(0.63)  LDD0734  [2]
 LDCM0418  CL3 HCT 116 C149(0.76)  LDD0735  [2]
 LDCM0419  CL30 HCT 116 C149(1.10); C182(0.65)  LDD0736  [2]
 LDCM0420  CL31 HCT 116 C149(1.00); C182(1.19)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C149(0.72); C182(1.12)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C149(0.71); C182(0.97)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C149(0.77); C182(1.46)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C149(0.85); C182(1.02)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C149(0.84); C182(0.95)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C149(0.95); C182(1.10)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C149(0.78); C182(0.99)  LDD0745  [2]
 LDCM0429  CL4 HCT 116 C149(1.02)  LDD0746  [2]
 LDCM0430  CL40 HCT 116 C149(0.74); C182(0.91)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C149(0.76); C182(0.92)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C149(0.87); C182(0.91)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C149(0.79); C182(1.26)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C149(0.76); C182(0.91)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C149(0.83); C182(1.08)  LDD0752  [2]
 LDCM0436  CL46 HCT 116 C149(0.73)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C149(0.77)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C149(0.83)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C149(1.02)  LDD0756  [2]
 LDCM0440  CL5 HCT 116 C149(0.90)  LDD0757  [2]
 LDCM0441  CL50 HCT 116 C149(1.02)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C149(0.91)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C149(0.93)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C149(0.81)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C149(0.90)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C149(0.94)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C149(0.78)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C149(0.78)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C149(0.88)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C149(0.83)  LDD0767  [2]
 LDCM0451  CL6 HCT 116 C149(0.93)  LDD0768  [2]
 LDCM0452  CL60 HCT 116 C149(0.83)  LDD0769  [2]
 LDCM0453  CL61 HCT 116 C149(0.94); C182(1.08)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C149(0.92); C182(1.08)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C149(0.79); C182(0.94)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C149(0.86); C182(0.86)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C149(0.89); C182(1.18)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C149(0.91); C182(0.89)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C149(0.85); C182(1.06)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C149(0.86); C182(0.82)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C149(0.73); C182(0.59)  LDD0778  [2]
 LDCM0462  CL7 HCT 116 C149(0.87)  LDD0779  [2]
 LDCM0463  CL70 HCT 116 C149(0.83); C182(0.79)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C149(0.85); C182(0.90)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C149(0.96); C182(1.02)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C149(0.86); C182(0.94)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C149(0.90); C182(0.93)  LDD0784  [2]
 LDCM0469  CL76 HCT 116 C149(0.71); C182(0.60)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C149(0.77); C182(0.66)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C149(0.75); C182(0.82)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C149(0.73); C182(0.73)  LDD0789  [2]
 LDCM0473  CL8 HCT 116 C149(0.79)  LDD0790  [2]
 LDCM0474  CL80 HCT 116 C149(0.56); C182(0.51)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C149(0.91); C182(0.74)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C149(0.80); C182(0.65)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C149(0.80); C182(0.60)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C149(0.90); C182(0.74)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C149(0.83); C182(0.76)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C149(0.95); C182(1.04)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C149(0.99); C182(0.68)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C149(0.78); C182(0.67)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C149(1.01); C182(0.85)  LDD0800  [2]
 LDCM0484  CL9 HCT 116 C149(0.83)  LDD0801  [2]
 LDCM0485  CL90 HCT 116 C149(1.24); C182(1.50)  LDD0802  [2]
 LDCM0486  CL91 HCT 116 C149(0.94)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C149(1.03)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C149(1.19)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C149(1.21)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C149(1.20)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C149(1.19)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C149(1.28)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C149(1.05)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C149(1.10)  LDD0811  [2]
 LDCM0625  F8 Ramos C70(0.62)  LDD2187  [9]
 LDCM0573  Fragment11 Ramos C70(3.06)  LDD2190  [9]
 LDCM0576  Fragment14 Ramos C70(0.91)  LDD2193  [9]
 LDCM0586  Fragment28 Ramos C70(0.73)  LDD2198  [9]
 LDCM0468  Fragment33 HCT 116 C149(0.81); C182(0.84)  LDD0785  [2]
 LDCM0566  Fragment4 Ramos C70(1.30)  LDD2184  [9]
 LDCM0427  Fragment51 HCT 116 C149(0.90); C182(0.99)  LDD0744  [2]
 LDCM0569  Fragment7 Ramos C70(1.00)  LDD2186  [9]
 LDCM0022  KB02 HEK-293T C182(1.00); C149(0.97)  LDD1492  [10]
 LDCM0023  KB03 HEK-293T C182(0.94); C149(1.05)  LDD1497  [10]
 LDCM0024  KB05 COLO792 C149(1.97); C182(2.01)  LDD3310  [11]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Methionine synthase (MTR) Vitamin-B12 dependent methionine synthase family Q99707

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Cyanocobalamin Small molecular drug DB00115
Hydroxocobalamin Small molecular drug DB00200

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
9 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
11 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840