Details of the Target

General Information of Target

Target ID LDTP10429
Target Name Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (NGLY1)
Gene Name NGLY1
Gene ID 55768
Synonyms
PNG1; Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; PNGase; hPNGase; EC 3.5.1.52; N-glycanase 1; Peptide:N-glycanase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAASVEQREGTIQVQGQALFFREALPGSGQARFSVLLLHGIRFSSETWQNLGTLHRLAQA
GYRAVAIDLPGLGHSKEAAAPAPIGELAPGSFLAAVVDALELGPPVVISPSLSGMYSLPF
LTAPGSQLPGFVPVAPICTDKINAANYASVKTPALIVYGDQDPMGQTSFEHLKQLPNHRV
LIMKGAGHPCYLDKPEEWHTGLLDFLQGLQ
Target Bioclass
Enzyme
Family
Transglutaminase-like superfamily, PNGase family
Subcellular location
Cytoplasm
Function
Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.
Uniprot ID
Q96IV0
Ensemble ID
ENST00000280700.10
HGNC ID
HGNC:17646
ChEMBL ID
CHEMBL4523429

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
EFO27 SNV: p.C393Y .
JURKAT SNV: p.A541T .
L428 SNV: p.Q492R .
MEWO SNV: p.R291Ter DBIA    Probe Info 
NCIH1155 SNV: p.T571I .
PATU8988T SNV: p.I54N DBIA    Probe Info 
RL952 SNV: p.Q574Ter .
TE4 SNV: p.E19Ter .
TOV21G SNV: p.L363F DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.75  LDD0402  [1]
IPM
 Probe Info  		N.A.  LDD0241  [2]
BTD
 Probe Info  		C197(0.73)  LDD2109  [3]
DBIA
 Probe Info  		C283(0.91); C286(0.91)  LDD0078  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		C197(0.00); C496(0.00); C393(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		C484(0.00); C197(0.00); C393(0.00)  LDD0037  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
TFBX
 Probe Info  		N.A.  LDD0148  [7]
W1
 Probe Info  		N.A.  LDD0236  [2]
AOyne
 Probe Info  		14.30  LDD0443  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C197(1.14)  LDD2117  [3]
 LDCM0214  AC1 HEK-293T C352(1.22); C359(1.23); C283(1.04); C286(1.04)  LDD0812  [4]
 LDCM0215  AC10 HEK-293T C359(1.18); C283(1.00); C286(1.00); C355(1.16)  LDD0813  [4]
 LDCM0216  AC100 HCT 116 C283(0.53); C286(0.53)  LDD0533  [4]
 LDCM0217  AC101 HCT 116 C283(0.63); C286(0.63)  LDD0534  [4]
 LDCM0218  AC102 HCT 116 C283(0.64); C286(0.64)  LDD0535  [4]
 LDCM0219  AC103 HCT 116 C283(0.85); C286(0.85)  LDD0536  [4]
 LDCM0220  AC104 HCT 116 C283(0.48); C286(0.48)  LDD0537  [4]
 LDCM0221  AC105 HCT 116 C283(0.58); C286(0.58)  LDD0538  [4]
 LDCM0222  AC106 HCT 116 C283(0.65); C286(0.65)  LDD0539  [4]
 LDCM0223  AC107 HCT 116 C283(0.69); C286(0.69)  LDD0540  [4]
 LDCM0224  AC108 HCT 116 C283(0.61); C286(0.61)  LDD0541  [4]
 LDCM0225  AC109 HCT 116 C283(0.74); C286(0.74)  LDD0542  [4]
 LDCM0226  AC11 HEK-293T C359(1.00); C283(0.81); C286(0.81); C355(1.15)  LDD0824  [4]
 LDCM0227  AC110 HCT 116 C283(0.78); C286(0.78)  LDD0544  [4]
 LDCM0228  AC111 HCT 116 C283(0.73); C286(0.73)  LDD0545  [4]
 LDCM0229  AC112 HCT 116 C283(0.57); C286(0.57)  LDD0546  [4]
 LDCM0230  AC113 HCT 116 C283(0.52); C286(0.52)  LDD0547  [4]
 LDCM0231  AC114 HCT 116 C283(0.48); C286(0.48)  LDD0548  [4]
 LDCM0232  AC115 HCT 116 C283(0.39); C286(0.39)  LDD0549  [4]
 LDCM0233  AC116 HCT 116 C283(0.48); C286(0.48)  LDD0550  [4]
 LDCM0234  AC117 HCT 116 C283(0.51); C286(0.51)  LDD0551  [4]
 LDCM0235  AC118 HCT 116 C283(0.45); C286(0.45)  LDD0552  [4]
 LDCM0236  AC119 HCT 116 C283(0.38); C286(0.38)  LDD0553  [4]
 LDCM0237  AC12 HEK-293T C359(1.24); C283(0.84); C286(0.84); C355(1.01)  LDD0835  [4]
 LDCM0238  AC120 HCT 116 C283(0.78); C286(0.78)  LDD0555  [4]
 LDCM0239  AC121 HCT 116 C283(0.39); C286(0.39)  LDD0556  [4]
 LDCM0240  AC122 HCT 116 C283(0.60); C286(0.60)  LDD0557  [4]
 LDCM0241  AC123 HCT 116 C283(0.66); C286(0.66)  LDD0558  [4]
 LDCM0242  AC124 HCT 116 C283(0.43); C286(0.43)  LDD0559  [4]
 LDCM0243  AC125 HCT 116 C283(1.84); C286(1.84)  LDD0560  [4]
 LDCM0244  AC126 HCT 116 C283(0.43); C286(0.43)  LDD0561  [4]
 LDCM0245  AC127 HCT 116 C283(0.44); C286(0.44)  LDD0562  [4]
 LDCM0246  AC128 HEK-293T C283(0.89); C286(1.07)  LDD0844  [4]
 LDCM0247  AC129 HEK-293T C283(0.97); C286(0.93)  LDD0845  [4]
 LDCM0249  AC130 HEK-293T C283(0.91); C286(0.96)  LDD0847  [4]
 LDCM0250  AC131 HEK-293T C283(1.07); C286(1.15)  LDD0848  [4]
 LDCM0251  AC132 HEK-293T C283(1.22); C286(1.05)  LDD0849  [4]
 LDCM0252  AC133 HEK-293T C283(1.17); C286(1.20)  LDD0850  [4]
 LDCM0253  AC134 HEK-293T C283(1.21); C286(1.21)  LDD0851  [4]
 LDCM0254  AC135 HEK-293T C283(0.91); C286(1.16)  LDD0852  [4]
 LDCM0255  AC136 HEK-293T C283(0.68); C286(1.00)  LDD0853  [4]
 LDCM0256  AC137 HEK-293T C283(0.60); C286(0.98)  LDD0854  [4]
 LDCM0257  AC138 HEK-293T C283(1.22); C286(1.28)  LDD0855  [4]
 LDCM0258  AC139 HEK-293T C283(0.59); C286(0.94)  LDD0856  [4]
 LDCM0259  AC14 HEK-293T C359(1.32); C283(1.02); C286(1.02); C355(1.14)  LDD0857  [4]
 LDCM0260  AC140 HEK-293T C283(0.91); C286(1.07)  LDD0858  [4]
 LDCM0261  AC141 HEK-293T C283(0.82); C286(1.04)  LDD0859  [4]
 LDCM0262  AC142 HEK-293T C283(0.59); C286(0.89)  LDD0860  [4]
 LDCM0263  AC143 HCT 116 C283(0.78); C286(0.78)  LDD0580  [4]
 LDCM0264  AC144 HCT 116 C283(0.78); C286(0.78)  LDD0581  [4]
 LDCM0265  AC145 HCT 116 C283(1.17); C286(1.17)  LDD0582  [4]
 LDCM0266  AC146 HCT 116 C283(0.69); C286(0.69)  LDD0583  [4]
 LDCM0267  AC147 HCT 116 C283(1.16); C286(1.16)  LDD0584  [4]
 LDCM0268  AC148 HCT 116 C283(0.74); C286(0.74)  LDD0585  [4]
 LDCM0269  AC149 HCT 116 C283(0.63); C286(0.63)  LDD0586  [4]
 LDCM0270  AC15 HEK-293T C359(1.71); C283(1.36); C286(1.36); C355(1.73)  LDD0868  [4]
 LDCM0271  AC150 HCT 116 C283(0.86); C286(0.86)  LDD0588  [4]
 LDCM0272  AC151 HCT 116 C283(0.90); C286(0.90)  LDD0589  [4]
 LDCM0273  AC152 HCT 116 C283(0.81); C286(0.81)  LDD0590  [4]
 LDCM0274  AC153 HCT 116 C283(0.83); C286(0.83)  LDD0591  [4]
 LDCM0621  AC154 HCT 116 C283(0.78); C286(0.78)  LDD2158  [4]
 LDCM0622  AC155 HCT 116 C283(0.96); C286(0.96)  LDD2159  [4]
 LDCM0623  AC156 HCT 116 C283(1.11); C286(1.11)  LDD2160  [4]
 LDCM0624  AC157 HCT 116 C283(1.20); C286(1.20)  LDD2161  [4]
 LDCM0276  AC17 HCT 116 C352(1.14); C359(1.14); C283(1.22); C286(1.22)  LDD0593  [4]
 LDCM0277  AC18 HCT 116 C352(0.43); C359(0.43); C283(1.11); C286(1.11)  LDD0594  [4]
 LDCM0278  AC19 HCT 116 C352(0.84); C359(0.84); C283(0.94); C286(0.94)  LDD0595  [4]
 LDCM0279  AC2 HEK-293T C352(1.12); C359(1.10); C283(1.05); C286(1.05)  LDD0877  [4]
 LDCM0280  AC20 HCT 116 C352(0.85); C359(0.85); C283(1.09); C286(1.09)  LDD0597  [4]
 LDCM0281  AC21 HCT 116 C352(1.05); C359(1.05); C283(1.14); C286(1.14)  LDD0598  [4]
 LDCM0282  AC22 HCT 116 C352(0.94); C359(0.94); C283(1.33); C286(1.33)  LDD0599  [4]
 LDCM0283  AC23 HCT 116 C283(0.85); C286(0.85); C352(1.41); C359(1.41)  LDD0600  [4]
 LDCM0284  AC24 HCT 116 C283(1.05); C286(1.05); C352(1.39); C359(1.39)  LDD0601  [4]
 LDCM0285  AC25 HCT 116 C283(0.75); C286(0.75); C352(0.75); C359(0.75)  LDD0602  [4]
 LDCM0286  AC26 HCT 116 C283(0.58); C286(0.58); C352(0.82); C359(0.82)  LDD0603  [4]
 LDCM0287  AC27 HCT 116 C283(0.66); C286(0.66); C352(0.70); C359(0.70)  LDD0604  [4]
 LDCM0288  AC28 HCT 116 C283(0.58); C286(0.58); C352(0.68); C359(0.68)  LDD0605  [4]
 LDCM0289  AC29 HCT 116 C352(0.69); C359(0.69); C283(0.72); C286(0.72)  LDD0606  [4]
 LDCM0290  AC3 HEK-293T C352(1.02); C359(0.86); C283(0.82); C286(0.82)  LDD0888  [4]
 LDCM0291  AC30 HCT 116 C283(0.61); C286(0.61); C352(0.79); C359(0.79)  LDD0608  [4]
 LDCM0292  AC31 HCT 116 C283(0.60); C286(0.60); C352(0.61); C359(0.61)  LDD0609  [4]
 LDCM0293  AC32 HCT 116 C283(0.54); C286(0.54); C352(0.69); C359(0.69)  LDD0610  [4]
 LDCM0294  AC33 HCT 116 C352(0.70); C359(0.70); C283(0.85); C286(0.85)  LDD0611  [4]
 LDCM0295  AC34 HCT 116 C283(0.88); C286(0.88); C352(0.92); C359(0.92)  LDD0612  [4]
 LDCM0296  AC35 HCT 116 C352(0.75); C359(0.75)  LDD0613  [4]
 LDCM0297  AC36 HCT 116 C352(0.86); C359(0.86)  LDD0614  [4]
 LDCM0298  AC37 HCT 116 C352(1.29); C359(1.29)  LDD0615  [4]
 LDCM0299  AC38 HCT 116 C352(1.50); C359(1.50)  LDD0616  [4]
 LDCM0300  AC39 HCT 116 C352(1.19); C359(1.19)  LDD0617  [4]
 LDCM0301  AC4 HEK-293T C352(1.08); C359(1.13); C283(0.92); C286(0.92)  LDD0899  [4]
 LDCM0302  AC40 HCT 116 C352(0.91); C359(0.91)  LDD0619  [4]
 LDCM0303  AC41 HCT 116 C352(1.20); C359(1.20)  LDD0620  [4]
 LDCM0304  AC42 HCT 116 C352(1.05); C359(1.05)  LDD0621  [4]
 LDCM0305  AC43 HCT 116 C352(1.16); C359(1.16)  LDD0622  [4]
 LDCM0306  AC44 HCT 116 C352(0.90); C359(0.90)  LDD0623  [4]
 LDCM0307  AC45 HCT 116 C352(1.20); C359(1.20)  LDD0624  [4]
 LDCM0308  AC46 HEK-293T C283(0.92); C286(1.10); C309(1.27); C355(1.06)  LDD0906  [4]
 LDCM0309  AC47 HEK-293T C283(1.01); C286(1.45); C309(0.95); C355(1.65)  LDD0907  [4]
 LDCM0310  AC48 HEK-293T C283(0.72); C286(0.91); C309(1.08); C355(1.81)  LDD0908  [4]
 LDCM0311  AC49 HEK-293T C283(0.78); C286(1.04); C309(0.90); C355(1.70)  LDD0909  [4]
 LDCM0312  AC5 HEK-293T C352(1.06); C359(1.17); C283(1.29); C286(1.29)  LDD0910  [4]
 LDCM0313  AC50 HEK-293T C283(0.83); C286(1.18); C309(1.05); C355(1.37)  LDD0911  [4]
 LDCM0314  AC51 HEK-293T C283(0.62); C286(0.79); C309(1.15); C355(1.40)  LDD0912  [4]
 LDCM0315  AC52 HEK-293T C283(0.83); C286(1.26); C309(0.96); C355(1.67)  LDD0913  [4]
 LDCM0316  AC53 HEK-293T C283(0.73); C286(0.79); C309(1.12); C355(1.84)  LDD0914  [4]
 LDCM0317  AC54 HEK-293T C283(0.77); C286(1.06); C309(1.09); C355(1.27)  LDD0915  [4]
 LDCM0318  AC55 HEK-293T C283(0.73); C286(0.95); C309(0.88); C355(1.38)  LDD0916  [4]
 LDCM0319  AC56 HEK-293T C283(0.75); C286(0.97); C309(1.04); C355(0.99)  LDD0917  [4]
 LDCM0320  AC57 HCT 116 C283(0.55); C286(0.55)  LDD0637  [4]
 LDCM0321  AC58 HCT 116 C283(0.96); C286(0.96)  LDD0638  [4]
 LDCM0322  AC59 HCT 116 C283(0.61); C286(0.61)  LDD0639  [4]
 LDCM0323  AC6 HEK-293T C359(1.02); C283(1.43); C286(1.43); C355(0.78)  LDD0921  [4]
 LDCM0324  AC60 HCT 116 C283(0.45); C286(0.45)  LDD0641  [4]
 LDCM0325  AC61 HCT 116 C283(0.59); C286(0.59)  LDD0642  [4]
 LDCM0326  AC62 HCT 116 C283(0.63); C286(0.63)  LDD0643  [4]
 LDCM0327  AC63 HCT 116 C283(0.41); C286(0.41)  LDD0644  [4]
 LDCM0328  AC64 HCT 116 C283(0.49); C286(0.49)  LDD0645  [4]
 LDCM0329  AC65 HCT 116 C283(0.33); C286(0.33)  LDD0646  [4]
 LDCM0330  AC66 HCT 116 C283(0.36); C286(0.36)  LDD0647  [4]
 LDCM0331  AC67 HCT 116 C283(0.31); C286(0.31)  LDD0648  [4]
 LDCM0332  AC68 HCT 116 C283(0.82); C286(0.82); C352(1.06); C359(1.06)  LDD0649  [4]
 LDCM0333  AC69 HCT 116 C283(0.79); C286(0.79); C352(1.28); C359(1.28)  LDD0650  [4]
 LDCM0334  AC7 HEK-293T C359(1.08); C283(1.18); C286(1.18); C355(1.83)  LDD0932  [4]
 LDCM0335  AC70 HCT 116 C283(0.74); C286(0.74); C352(1.33); C359(1.33)  LDD0652  [4]
 LDCM0336  AC71 HCT 116 C283(0.73); C286(0.73); C352(1.36); C359(1.36)  LDD0653  [4]
 LDCM0337  AC72 HCT 116 C283(0.84); C286(0.84); C352(1.57); C359(1.57)  LDD0654  [4]
 LDCM0338  AC73 HCT 116 C283(1.03); C286(1.03); C352(1.18); C359(1.18)  LDD0655  [4]
 LDCM0339  AC74 HCT 116 C283(0.98); C286(0.98); C352(1.09); C359(1.09)  LDD0656  [4]
 LDCM0340  AC75 HCT 116 C283(0.93); C286(0.93); C352(1.25); C359(1.25)  LDD0657  [4]
 LDCM0341  AC76 HCT 116 C283(0.85); C286(0.85); C352(1.34); C359(1.34)  LDD0658  [4]
 LDCM0342  AC77 HCT 116 C283(0.71); C286(0.71); C352(1.05); C359(1.05)  LDD0659  [4]
 LDCM0343  AC78 HCT 116 C283(0.76); C286(0.76); C352(1.34); C359(1.34)  LDD0660  [4]
 LDCM0344  AC79 HCT 116 C283(0.71); C286(0.71); C352(1.33); C359(1.33)  LDD0661  [4]
 LDCM0345  AC8 HEK-293T C359(1.53); C283(1.35); C286(1.35); C355(1.17)  LDD0943  [4]
 LDCM0346  AC80 HCT 116 C283(0.79); C286(0.79); C352(1.26); C359(1.26)  LDD0663  [4]
 LDCM0347  AC81 HCT 116 C283(0.88); C286(0.88); C352(1.17); C359(1.17)  LDD0664  [4]
 LDCM0348  AC82 HCT 116 C283(0.96); C286(0.96); C352(1.49); C359(1.49)  LDD0665  [4]
 LDCM0349  AC83 HCT 116 C283(0.81); C286(0.81)  LDD0666  [4]
 LDCM0350  AC84 HCT 116 C283(0.83); C286(0.83)  LDD0667  [4]
 LDCM0351  AC85 HCT 116 C283(0.76); C286(0.76)  LDD0668  [4]
 LDCM0352  AC86 HCT 116 C283(0.83); C286(0.83)  LDD0669  [4]
 LDCM0353  AC87 HCT 116 C283(0.86); C286(0.86)  LDD0670  [4]
 LDCM0354  AC88 HCT 116 C283(0.83); C286(0.83)  LDD0671  [4]
 LDCM0355  AC89 HCT 116 C283(0.85); C286(0.85)  LDD0672  [4]
 LDCM0357  AC90 HCT 116 C283(1.43); C286(1.43)  LDD0674  [4]
 LDCM0358  AC91 HCT 116 C283(0.79); C286(0.79)  LDD0675  [4]
 LDCM0359  AC92 HCT 116 C283(0.75); C286(0.75)  LDD0676  [4]
 LDCM0360  AC93 HCT 116 C283(1.00); C286(1.00)  LDD0677  [4]
 LDCM0361  AC94 HCT 116 C283(0.94); C286(0.94)  LDD0678  [4]
 LDCM0362  AC95 HCT 116 C283(0.88); C286(0.88)  LDD0679  [4]
 LDCM0363  AC96 HCT 116 C283(0.71); C286(0.71)  LDD0680  [4]
 LDCM0364  AC97 HCT 116 C283(0.90); C286(0.90)  LDD0681  [4]
 LDCM0365  AC98 HCT 116 C283(0.37); C286(0.37)  LDD0682  [4]
 LDCM0366  AC99 HCT 116 C283(0.87); C286(0.87)  LDD0683  [4]
 LDCM0248  AKOS034007472 HEK-293T C359(1.13); C283(1.24); C286(1.24); C355(1.10)  LDD0846  [4]
 LDCM0356  AKOS034007680 HEK-293T C359(1.56); C283(0.86); C286(0.86); C355(1.50)  LDD0954  [4]
 LDCM0275  AKOS034007705 HEK-293T C359(1.25); C283(0.93); C286(0.93); C355(1.52)  LDD0873  [4]
 LDCM0020  ARS-1620 HCC44 C283(0.91); C286(0.91)  LDD0078  [4]
 LDCM0102  BDHI 8 Jurkat C283(17.89); C286(17.89)  LDD0204  [9]
 LDCM0367  CL1 HEK-293T C283(0.84); C286(0.83)  LDD0965  [4]
 LDCM0368  CL10 HEK-293T C283(0.81); C286(1.21)  LDD0966  [4]
 LDCM0369  CL100 HEK-293T C352(1.24); C359(1.53); C283(1.17); C286(1.17)  LDD0967  [4]
 LDCM0370  CL101 HEK-293T C359(1.53); C283(1.21); C286(1.21); C355(1.61)  LDD0968  [4]
 LDCM0371  CL102 HEK-293T C359(1.79); C283(0.97); C286(0.97); C355(1.91)  LDD0969  [4]
 LDCM0372  CL103 HEK-293T C359(1.40); C283(1.07); C286(1.07); C355(1.42)  LDD0970  [4]
 LDCM0373  CL104 HEK-293T C359(1.64); C283(1.04); C286(1.04); C355(1.98)  LDD0971  [4]
 LDCM0374  CL105 HCT 116 C283(0.91); C286(0.91); C352(1.13); C359(1.13)  LDD0691  [4]
 LDCM0375  CL106 HCT 116 C352(0.90); C359(0.90); C283(0.95); C286(0.95)  LDD0692  [4]
 LDCM0376  CL107 HCT 116 C283(0.78); C286(0.78); C352(1.07); C359(1.07)  LDD0693  [4]
 LDCM0377  CL108 HCT 116 C283(0.86); C286(0.86); C352(1.06); C359(1.06)  LDD0694  [4]
 LDCM0378  CL109 HCT 116 C283(0.78); C286(0.78); C352(0.97); C359(0.97)  LDD0695  [4]
 LDCM0379  CL11 HEK-293T C283(0.72); C286(1.04)  LDD0977  [4]
 LDCM0380  CL110 HCT 116 C283(0.82); C286(0.82); C352(1.08); C359(1.08)  LDD0697  [4]
 LDCM0381  CL111 HCT 116 C352(0.77); C359(0.77); C283(0.93); C286(0.93)  LDD0698  [4]
 LDCM0382  CL112 HCT 116 C352(0.64); C359(0.64); C283(0.66); C286(0.66)  LDD0699  [4]
 LDCM0383  CL113 HCT 116 C283(0.56); C286(0.56); C352(0.60); C359(0.60)  LDD0700  [4]
 LDCM0384  CL114 HCT 116 C283(0.54); C286(0.54); C352(0.85); C359(0.85)  LDD0701  [4]
 LDCM0385  CL115 HCT 116 C283(0.59); C286(0.59); C352(0.83); C359(0.83)  LDD0702  [4]
 LDCM0386  CL116 HCT 116 C283(0.64); C286(0.64); C352(0.74); C359(0.74)  LDD0703  [4]
 LDCM0387  CL117 HCT 116 C352(1.68); C359(1.68)  LDD0704  [4]
 LDCM0388  CL118 HCT 116 C352(1.27); C359(1.27)  LDD0705  [4]
 LDCM0389  CL119 HCT 116 C352(1.00); C359(1.00)  LDD0706  [4]
 LDCM0390  CL12 HEK-293T C283(0.75); C286(0.95)  LDD0988  [4]
 LDCM0391  CL120 HCT 116 C352(1.23); C359(1.23)  LDD0708  [4]
 LDCM0392  CL121 HEK-293T C283(0.92); C286(1.31); C309(1.08); C355(1.68)  LDD0990  [4]
 LDCM0393  CL122 HEK-293T C283(0.70); C286(0.84); C309(1.18); C355(1.48)  LDD0991  [4]
 LDCM0394  CL123 HEK-293T C283(0.91); C286(1.19); C309(0.98); C355(1.72)  LDD0992  [4]
 LDCM0395  CL124 HEK-293T C283(0.78); C286(1.01); C309(0.98); C355(1.31)  LDD0993  [4]
 LDCM0396  CL125 HCT 116 C283(0.80); C286(0.80)  LDD0713  [4]
 LDCM0397  CL126 HCT 116 C283(0.92); C286(0.92)  LDD0714  [4]
 LDCM0398  CL127 HCT 116 C283(0.92); C286(0.92)  LDD0715  [4]
 LDCM0399  CL128 HCT 116 C283(0.70); C286(0.70)  LDD0716  [4]
 LDCM0400  CL13 HEK-293T C283(0.74); C286(0.98)  LDD0998  [4]
 LDCM0401  CL14 HEK-293T C283(0.91); C286(1.32)  LDD0999  [4]
 LDCM0402  CL15 HEK-293T C283(0.80); C286(1.23)  LDD1000  [4]
 LDCM0403  CL16 HEK-293T C352(0.89); C359(0.90); C309(0.85); C319(0.85)  LDD1001  [4]
 LDCM0404  CL17 HEK-293T C352(2.69); C359(0.53); C309(0.99); C319(0.99)  LDD1002  [4]
 LDCM0405  CL18 HEK-293T C352(1.13); C359(0.83); C309(1.29); C319(1.29)  LDD1003  [4]
 LDCM0406  CL19 HEK-293T C352(1.15); C359(0.81); C309(0.95); C319(0.95)  LDD1004  [4]
 LDCM0407  CL2 HEK-293T C283(0.86); C286(1.10)  LDD1005  [4]
 LDCM0408  CL20 HEK-293T C352(0.99); C359(0.82); C309(0.89); C319(0.89)  LDD1006  [4]
 LDCM0409  CL21 HEK-293T C352(5.71); C359(0.76); C309(0.91); C319(0.91)  LDD1007  [4]
 LDCM0410  CL22 HEK-293T C352(1.15); C359(0.61); C309(1.08); C319(1.08)  LDD1008  [4]
 LDCM0411  CL23 HEK-293T C352(0.95); C359(0.61); C309(0.89); C319(0.89)  LDD1009  [4]
 LDCM0412  CL24 HEK-293T C352(1.14); C359(0.71); C309(0.78); C319(0.78)  LDD1010  [4]
 LDCM0413  CL25 HEK-293T C352(2.86); C359(0.48); C309(0.73); C319(0.73)  LDD1011  [4]
 LDCM0414  CL26 HEK-293T C352(1.04); C359(0.73); C309(0.76); C319(0.76)  LDD1012  [4]
 LDCM0415  CL27 HEK-293T C352(1.07); C359(0.76); C309(0.89); C319(0.89)  LDD1013  [4]
 LDCM0416  CL28 HEK-293T C352(1.19); C359(0.69); C309(0.93); C319(0.93)  LDD1014  [4]
 LDCM0417  CL29 HEK-293T C352(0.99); C359(0.71); C309(0.99); C319(0.99)  LDD1015  [4]
 LDCM0418  CL3 HEK-293T C283(1.02); C286(1.43)  LDD1016  [4]
 LDCM0419  CL30 HEK-293T C352(1.45); C359(1.36); C309(1.01); C319(1.01)  LDD1017  [4]
 LDCM0420  CL31 HEK-293T C352(1.19); C359(1.43); C283(1.04); C286(1.34)  LDD1018  [4]
 LDCM0421  CL32 HEK-293T C352(1.05); C359(1.06); C283(0.79); C286(0.98)  LDD1019  [4]
 LDCM0422  CL33 HEK-293T C352(6.47); C359(2.24); C283(1.02); C286(1.46)  LDD1020  [4]
 LDCM0423  CL34 HEK-293T C352(1.58); C359(1.23); C283(0.85); C286(0.95)  LDD1021  [4]
 LDCM0424  CL35 HEK-293T C352(1.07); C359(1.24); C283(0.77); C286(1.04)  LDD1022  [4]
 LDCM0425  CL36 HEK-293T C352(1.21); C359(1.30); C283(0.80); C286(0.94)  LDD1023  [4]
 LDCM0426  CL37 HEK-293T C352(1.25); C359(1.24); C283(0.80); C286(1.10)  LDD1024  [4]
 LDCM0428  CL39 HEK-293T C352(2.47); C359(2.57); C283(0.66); C286(1.00)  LDD1026  [4]
 LDCM0429  CL4 HEK-293T C283(1.22); C286(1.89)  LDD1027  [4]
 LDCM0430  CL40 HEK-293T C352(1.18); C359(1.42); C283(0.88); C286(1.23)  LDD1028  [4]
 LDCM0431  CL41 HEK-293T C352(1.99); C359(2.26); C283(0.82); C286(1.32)  LDD1029  [4]
 LDCM0432  CL42 HEK-293T C352(1.67); C359(1.98); C283(0.92); C286(1.45)  LDD1030  [4]
 LDCM0433  CL43 HEK-293T C352(1.74); C359(2.10); C283(0.68); C286(0.96)  LDD1031  [4]
 LDCM0434  CL44 HEK-293T C352(1.20); C359(1.38); C283(0.77); C286(1.00)  LDD1032  [4]
 LDCM0435  CL45 HEK-293T C352(2.14); C359(2.39); C283(0.89); C286(1.43)  LDD1033  [4]
 LDCM0436  CL46 HEK-293T C359(1.27); C309(0.96); C319(0.96); C355(1.15)  LDD1034  [4]
 LDCM0437  CL47 HEK-293T C359(1.02); C309(1.04); C319(1.04); C355(1.18)  LDD1035  [4]
 LDCM0438  CL48 HEK-293T C359(0.99); C309(1.21); C319(1.21); C355(0.93)  LDD1036  [4]
 LDCM0439  CL49 HEK-293T C359(1.54); C309(0.90); C319(0.90); C355(1.07)  LDD1037  [4]
 LDCM0440  CL5 HEK-293T C283(0.96); C286(1.39)  LDD1038  [4]
 LDCM0441  CL50 HEK-293T C359(2.03); C309(0.99); C319(0.99); C355(2.02)  LDD1039  [4]
 LDCM0442  CL51 HEK-293T C359(1.71); C309(1.10); C319(1.10); C355(1.36)  LDD1040  [4]
 LDCM0443  CL52 HEK-293T C359(0.88); C309(0.88); C319(0.88); C355(0.58)  LDD1041  [4]
 LDCM0444  CL53 HEK-293T C359(1.34); C309(0.89); C319(0.89); C355(0.96)  LDD1042  [4]
 LDCM0445  CL54 HEK-293T C359(1.52); C309(1.13); C319(1.13); C355(1.17)  LDD1043  [4]
 LDCM0446  CL55 HEK-293T C359(1.11); C309(0.97); C319(0.97); C355(1.02)  LDD1044  [4]
 LDCM0447  CL56 HEK-293T C359(0.99); C309(1.05); C319(1.05); C355(0.89)  LDD1045  [4]
 LDCM0448  CL57 HEK-293T C359(1.45); C309(1.01); C319(1.01); C355(1.34)  LDD1046  [4]
 LDCM0449  CL58 HEK-293T C359(1.14); C309(0.81); C319(0.81); C355(1.14)  LDD1047  [4]
 LDCM0450  CL59 HEK-293T C359(1.40); C309(0.96); C319(0.96); C355(1.30)  LDD1048  [4]
 LDCM0451  CL6 HEK-293T C283(0.80); C286(1.04)  LDD1049  [4]
 LDCM0452  CL60 HEK-293T C359(1.89); C309(1.00); C319(1.00); C355(1.96)  LDD1050  [4]
 LDCM0453  CL61 HEK-293T C359(1.31); C283(1.37); C286(1.37); C355(1.18)  LDD1051  [4]
 LDCM0454  CL62 HEK-293T C359(1.10); C283(1.10); C286(1.10); C355(1.01)  LDD1052  [4]
 LDCM0455  CL63 HEK-293T C359(1.39); C283(1.47); C286(1.47); C355(1.21)  LDD1053  [4]
 LDCM0456  CL64 HEK-293T C359(4.00); C283(1.05); C286(1.05); C355(1.59)  LDD1054  [4]
 LDCM0457  CL65 HEK-293T C359(0.95); C283(0.95); C286(0.95); C355(1.00)  LDD1055  [4]
 LDCM0458  CL66 HEK-293T C359(1.24); C283(1.06); C286(1.06); C355(1.05)  LDD1056  [4]
 LDCM0459  CL67 HEK-293T C359(1.00); C283(1.29); C286(1.29); C355(1.31)  LDD1057  [4]
 LDCM0460  CL68 HEK-293T C359(1.71); C283(0.96); C286(0.96); C355(0.97)  LDD1058  [4]
 LDCM0461  CL69 HEK-293T C359(0.89); C283(1.21); C286(1.21); C355(1.02)  LDD1059  [4]
 LDCM0462  CL7 HEK-293T C283(1.13); C286(1.77)  LDD1060  [4]
 LDCM0463  CL70 HEK-293T C359(0.97); C283(1.29); C286(1.29); C355(1.63)  LDD1061  [4]
 LDCM0464  CL71 HEK-293T C359(1.53); C283(1.01); C286(1.01); C355(1.39)  LDD1062  [4]
 LDCM0465  CL72 HEK-293T C359(0.98); C283(1.44); C286(1.44); C355(1.16)  LDD1063  [4]
 LDCM0466  CL73 HEK-293T C359(1.90); C283(0.97); C286(0.97); C355(0.71)  LDD1064  [4]
 LDCM0467  CL74 HEK-293T C359(1.82); C283(1.12); C286(1.12); C355(1.53)  LDD1065  [4]
 LDCM0469  CL76 HCT 116 C352(1.19); C359(1.19)  LDD0786  [4]
 LDCM0470  CL77 HCT 116 C352(0.57); C359(0.57)  LDD0787  [4]
 LDCM0471  CL78 HCT 116 C352(0.77); C359(0.77)  LDD0788  [4]
 LDCM0472  CL79 HCT 116 C352(1.27); C359(1.27)  LDD0789  [4]
 LDCM0473  CL8 HEK-293T C283(1.09)  LDD1071  [4]
 LDCM0474  CL80 HCT 116 C352(0.70); C359(0.70)  LDD0791  [4]
 LDCM0475  CL81 HCT 116 C352(0.83); C359(0.83)  LDD0792  [4]
 LDCM0476  CL82 HCT 116 C352(1.09); C359(1.09)  LDD0793  [4]
 LDCM0477  CL83 HCT 116 C352(0.69); C359(0.69)  LDD0794  [4]
 LDCM0478  CL84 HCT 116 C352(1.04); C359(1.04)  LDD0795  [4]
 LDCM0479  CL85 HCT 116 C352(1.11); C359(1.11)  LDD0796  [4]
 LDCM0480  CL86 HCT 116 C352(0.74); C359(0.74)  LDD0797  [4]
 LDCM0481  CL87 HCT 116 C352(1.09); C359(1.09)  LDD0798  [4]
 LDCM0482  CL88 HCT 116 C352(1.27); C359(1.27)  LDD0799  [4]
 LDCM0483  CL89 HCT 116 C352(1.41); C359(1.41)  LDD0800  [4]
 LDCM0484  CL9 HEK-293T C283(0.75)  LDD1082  [4]
 LDCM0485  CL90 HCT 116 C352(0.67); C359(0.67)  LDD0802  [4]
 LDCM0486  CL91 HEK-293T C352(0.89); C359(1.16); C283(1.37); C286(1.37)  LDD1084  [4]
 LDCM0487  CL92 HEK-293T C352(2.86); C359(1.43); C283(0.83); C286(0.83)  LDD1085  [4]
 LDCM0488  CL93 HEK-293T C352(2.01); C359(1.25); C283(0.97); C286(0.97)  LDD1086  [4]
 LDCM0489  CL94 HEK-293T C352(1.28); C359(1.86); C283(0.74); C286(0.74)  LDD1087  [4]
 LDCM0490  CL95 HEK-293T C352(2.98); C359(3.28); C283(0.70); C286(0.70)  LDD1088  [4]
 LDCM0491  CL96 HEK-293T C352(1.30); C359(1.52); C283(2.06); C286(2.06)  LDD1089  [4]
 LDCM0492  CL97 HEK-293T C352(1.26); C359(1.45); C283(0.88); C286(0.88)  LDD1090  [4]
 LDCM0493  CL98 HEK-293T C352(1.37); C359(1.31); C283(0.81); C286(0.81)  LDD1091  [4]
 LDCM0494  CL99 HEK-293T C352(1.22); C359(1.34); C283(0.74); C286(0.74)  LDD1092  [4]
 LDCM0495  E2913 HEK-293T C484(1.01); C197(0.93); C250(0.75)  LDD1698  [10]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C197(3.07)  LDD1702  [3]
 LDCM0625  F8 Ramos C197(0.93)  LDD2187  [11]
 LDCM0572  Fragment10 Ramos C197(0.76)  LDD2189  [11]
 LDCM0573  Fragment11 Ramos C197(0.15); 8.41  LDD2190  [11]
 LDCM0574  Fragment12 Ramos C197(0.85)  LDD2191  [11]
 LDCM0575  Fragment13 Ramos C197(1.08)  LDD2192  [11]
 LDCM0576  Fragment14 Ramos C197(0.73); 0.79  LDD2193  [11]
 LDCM0579  Fragment20 Ramos C197(1.11)  LDD2194  [11]
 LDCM0580  Fragment21 Ramos C197(0.82)  LDD2195  [11]
 LDCM0582  Fragment23 Ramos C197(1.23)  LDD2196  [11]
 LDCM0578  Fragment27 Ramos C197(0.97)  LDD2197  [11]
 LDCM0586  Fragment28 Ramos C197(0.77); 1.19  LDD2198  [11]
 LDCM0587  Fragment29 Ramos C309(3.03)  LDD1476  [12]
 LDCM0588  Fragment30 Ramos C197(1.02)  LDD2199  [11]
 LDCM0589  Fragment31 Ramos C197(0.86)  LDD2200  [11]
 LDCM0590  Fragment32 Ramos C197(0.60)  LDD2201  [11]
 LDCM0468  Fragment33 HEK-293T C359(1.69); C283(1.90); C286(1.90); C355(1.28)  LDD1066  [4]
 LDCM0596  Fragment38 Ramos C197(1.00)  LDD2203  [11]
 LDCM0566  Fragment4 Ramos C197(1.00)  LDD2184  [11]
 LDCM0427  Fragment51 HEK-293T C352(1.07); C359(1.11); C283(1.09); C286(1.51)  LDD1025  [4]
 LDCM0610  Fragment52 Ramos C197(1.03)  LDD2204  [11]
 LDCM0614  Fragment56 Ramos C197(1.11)  LDD2205  [11]
 LDCM0569  Fragment7 Ramos C197(0.94); 1.67  LDD2186  [11]
 LDCM0571  Fragment9 Ramos C309(6.79)  LDD1464  [12]
 LDCM0022  KB02 HCT 116 C309(3.31)  LDD0080  [4]
 LDCM0023  KB03 HCT 116 C309(3.04)  LDD0081  [4]
 LDCM0024  KB05 HCT 116 C309(2.37)  LDD0082  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C197(0.73)  LDD2109  [3]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C197(0.84)  LDD2123  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C197(0.99)  LDD2127  [3]
 LDCM0021  THZ1 HCT 116 C283(1.06); C286(1.06)  LDD2173  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein GUCD1 (GUCD1) . Q96NT3
Tripartite motif-containing protein 54 (TRIM54) . Q9BYV2
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Paired box protein Pax-6 (PAX6) Paired homeobox family P26367
Neuronal PAS domain-containing protein 2 (NPAS2) . Q99743
Paired box protein Pax-5 (PAX5) . Q02548
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
UBX domain-containing protein 2B (UBXN2B) NSFL1C family Q14CS0
UV excision repair protein RAD23 homolog A (RAD23A) RAD23 family P54725
UV excision repair protein RAD23 homolog B (RAD23B) RAD23 family P54727
BRD4-interacting chromatin-remodeling complex-associated protein-like (BICRAL) . Q6AI39
TRAF-type zinc finger domain-containing protein 1 (TRAFD1) . O14545
Ubiquilin-1 (UBQLN1) . Q9UMX0
Vimentin-type intermediate filament-associated coiled-coil protein (VMAC) . Q2NL98

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
11 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
12 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.