Details of the Target

General Information of Target

Target ID LDTP01515
Target Name NADH dehydrogenase 1 beta subcomplex subunit 8, mitochondrial (NDUFB8)
Gene Name NDUFB8
Gene ID 4714
Synonyms
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; Complex I-ASHI; CI-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAVARAGVLGVQWLQRASRNVMPLGARTASHMTKDMFPGPYPRTPEERAAAAKKYNMRVE
DYEPYPDDGMGYGDYPKLPDRSQHERDPWYSWDQPGLRLNWGEPMHWHLDMYNRNRVDTS
PTPVSWHVMCMQLFGFLAFMIFMCWVGDVYPVYQPVGPKQYPYNNLYLERGGDPSKEPER
VVHYEI
Target Bioclass
Enzyme
Family
Complex I NDUFB8 subunit family
Subcellular location
Mitochondrion inner membrane
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Uniprot ID
O95169
Ensemble ID
ENST00000299166.9
HGNC ID
HGNC:7703
ChEMBL ID
CHEMBL2363065

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
MELHO SNV: p.V146G .
SKMEL3 SNV: p.Q15E .
SNGM SNV: p.D148G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TG42
 Probe Info  		4.41  LDD0043  [1]
TH211
 Probe Info  		Y90(13.70)  LDD0260  [2]
Probe 1
 Probe Info  		Y72(97.01); Y161(37.21)  LDD3495  [3]
Acrolein
 Probe Info  		N.A.  LDD0224  [4]
AOyne
 Probe Info  		14.70  LDD0443  [5]
PAL-AfBPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C040
 Probe Info  		9.06  LDD1740  [6]
C056
 Probe Info  		16.91  LDD1753  [6]
C112
 Probe Info  		19.97  LDD1799  [6]
C355
 Probe Info  		39.67  LDD2016  [6]
C356
 Probe Info  		15.24  LDD2017  [6]
C357
 Probe Info  		7.57  LDD2018  [6]
C360
 Probe Info  		5.74  LDD2021  [6]
C361
 Probe Info  		20.68  LDD2022  [6]
C390
 Probe Info  		25.46  LDD2049  [6]
C413
 Probe Info  		12.47  LDD2069  [6]
FFF probe11
 Probe Info  		20.00  LDD0471  [7]
FFF probe13
 Probe Info  		20.00  LDD0475  [7]
FFF probe14
 Probe Info  		20.00  LDD0477  [7]
FFF probe2
 Probe Info  		20.00  LDD0463  [7]
OEA-DA
 Probe Info  		3.24  LDD0046  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0109  NEM HeLa N.A.  LDD0224  [4]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Nadh Small molecular drug DB00157

References

1 Design and synthesis of tailored human caseinolytic protease P inhibitors. Chem Commun (Camb). 2018 Aug 28;54(70):9833-9836. doi: 10.1039/c8cc05265d.
Mass spectrometry data entry: PXD010277
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
7 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
8 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570