Probe Name	C340					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP03540	Heme oxygenase 2 (HMOX2)					26.0
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					12.8
LDTP05476	3-ketodihydrosphingosine reductase (KDSR)					8.9
LDTP12013	dCTP pyrophosphatase 1 (DCTPP1)					7.6
LDTP04285	Proteasome subunit beta type-2 (PSMB2)					7.4
LDTP04004	Lysosomal Pro-X carboxypeptidase (PRCP)					7.3
LDTP06618	ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP)					7.0
LDTP13358	Leucyl-cystinyl aminopeptidase (LNPEP)					6.7
LDTP05573	Peptidyl-prolyl cis-trans isomerase D (PPID)					6.0
LDTP11273	N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1)					5.5
LDTP04388	Palmitoyl-protein thioesterase 1 (PPT1)					5.4
LDTP02837	Beta-galactosidase (GLB1)					5.0
LDTP02359	Heme oxygenase 1 (HMOX1)					5.0

Target ID	Target Name					Binding Ratio
LDTP01059	Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B)					12.2
LDTP10913	Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A)					6.3
LDTP14952	Mpv17-like protein 2 (MPV17L2)					6.1
LDTP09547	Scavenger receptor class B member 1 (SCARB1)					6.1
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					5.1

Target ID	Target Name					Binding Ratio
LDTP13311	Poly(U)-binding-splicing factor PUF60 (PUF60)					12.0
LDTP15623	Neuferricin (CYB5D2)					9.6
LDTP05160	Nucleobindin-2 (NUCB2)					7.0
LDTP01075	Protein CutA (CUTA)					5.5

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587