Details of the Target

General Information of Target

Target ID LDTP14009
Target Name Serine/threonine-protein kinase WNK2 (WNK2)
Gene Name WNK2
Gene ID 65268
Synonyms
KIAA1760; PRKWNK2; SDCCAG43; Serine/threonine-protein kinase WNK2; EC 2.7.11.1; Antigen NY-CO-43; Protein kinase lysine-deficient 2; Protein kinase with no lysine 2; Serologically defined colon cancer antigen 43
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDPEEQELLNDYRYRSYSSVIEKALRNFESSSEWADLISSLGKLNKALQSNLRYSLLPRR
LLISKRLAQCLHPALPSGVHLKALETYEIIFKIVGTKWLAKDLFLYSCGLFPLLAHAAVS
VRPVLLTLYEKYFLPLQKLLLPSLQAFIVGLLPGLEEGSEISDRTDALLLRLSLVVGKEV
FYTALWGSVLASPSIRLPASVFVVGHINRDAPGREQKYMLGTNHQLTVKSLRASLLDSNV
LVQRNNLEIVLFFFPFYTCLDSNERAIPLLRSDIVRILSAATQTLLRRDMSLNRRLYAWL
LGSDIKGNTVVPESEISNSYEDQSSYFFEKYSKDLLVEGLAEILHQKFIDADVEERHHAY
LKPFRVLISLLDKPEIGPQVVGNLFLEVIRAFYSYCRDALGSDLKLSYTQSGNSLISAIK
ENRNASEIVKTVNLLITSLSTDFLWDYMTRCFEECFRPVKQRYSVRNSVSPPPTVSELCA
LLVFLLDVIPLELYSEVQTQYLPQVLGCLVQPLAEDMEALSLPELTHALKTCFKVLSKVQ
MPPSYLDTESTSGTSSPVKGENGKIILETKAVIPGDEDASFPPLKSEDSGIGLSASSPEL
SEHLRVPRVSLERDDVWKKGGSMQRTFLCIQELIANFASKNIFGVQLTASGEESKSEEPA
GKRDRDGTQSLAANDSSRKNSWEPKPITVPQFKQMLSDLFTARGSPFKTKSSESPSSSPS
SPARKNGGEWDVEKVVIDLGGSREERREAFAAACHLLLDCATFPVYLSEEETEQLCATLF
QLPGAGDSSFPSWLKSLMTICCCVTDCYLQNVAISTLLEVINHSQSLALVIEDKMKRYKS
SGHNPFFGKLQMVTVPPIAPGILKVIAEKTDFYQRVARVLWNQLNKETREHHVTCVELFY
RLHCLAPTANICEDIICHALLDPDKGTRLEALFRFSVIWHLTREIQGSRVTSHNRSFDRS
LFVVLDSLACTDGAIGAAAQGWLVRALSLGDVARILEPVLLLLLQPKTQRTSIHCLKQEN
SADDLHRWFNRKKTSFREACAVPEPQESGSEEHLPLSQFTTVDREAIWAEVEKEPEKYPL
RGELSEEELPYYVELPDRTAHGAPDSSEHTESADTSSCHTDSENTSSFSSPSHDLQELSN
EENCCAPIPMGGRAYPKRSALLAAFQSESFKAGAKLSLVRVDSDKTQASESFSSDEEADL
ELQALTTSRLLKQQRERQEAVEALFKHILLYLQPYDSRRVLYAFSVLEAVLKTNPKEFIE
AVSRTSMDTSSTAHLNLISNLLARHQEALIGQSFYGKLQTQVPNVCPHSLLLELLTYLCL
SFLRSYYPCYLKVSHRDILGNRDVQVKSVEVLIRIMMQLVSVAKSSEGKNVEFIHSLLQR
CKVQEFVLLSLSASMYTSQKRYGLATAHHGRALPEDSLFEESLINLGQDQIWSEHPLQIE
LLKLLQVLIVLEHHLGRAHEEAENQPDLSREWQRALNFQQAISALQYVQPHPLTSQGLLV
SAVVRGLQPAYGYGMHPAWVSLVTHSLPYFGKSLGWTVTPFVVQICKNLDDLVKQYESES
VKLSVSTTSKRENISPDYPLTLLEGLTTISHFCLLEQANQNKKTMAAGDPANLRNARNAI
LEELPRTVNTMALLWNVLRKEETQKRPVDLLGATKGSSSVYFKTTKTIRQKILDFLNPLT
AHLGVQLTAAVAAVWSRKKAQRHSKMKIIPTASASQLTLVDLVCALSTLQTDTLLHLVKE
VVKRPPQVKGGDEKSPLVDIPVLQFCYAFLQRLPVPALQENFSSLLGVLKESVQLNLAPP
GYFLLLSMLNDFVTRTPNLENKKDQKDLQEITQKILEAVGNIAGSSLEQTSWLSRNLEVK
AQPQASLEESDAEEDLYDAAAASAMVSSSAPSVYSVQALSLLAEVLASLLDMVYRSDEKE
KAVPLISRLLYYVFPYLRNHSAYNAPSFRAGAQLLSSLSGYAYTKRAWRKEVLELFLDPA
FFQMDTSCVHWKSIIDHLLTHEKTMFKDLMNMQSSSLKLFSSFEQKAMLLKRQAFAVFSG
ELDQYHLYLPLIQERLTDNLRVGQTSIVAAQMFLFFRVLLLRISPQHLTSLWPIMVSELI
QTFTQLEEDLKDEDESLRSTNKVNRTKVSVPDANGPSVGEIPQSELILYLSACKFLDTAL
SFPPDKMPLFQIYRWAFIPEVDTEGPAFLSDVEENHQECKPHTVRILELLKLKFGEISSS
DEITMKSEFPLLRQHSVSSIRQLMPFFMTLNGAFKTQRQLPADSPGTPFLDFPVTDSPRI
LKQLEECIEYDFLEHPEC
Target Bioclass
Enzyme
Family
Protein kinase superfamily, Ser/Thr protein kinase family, WNK subfamily
Subcellular location
Cytoplasm
Function
Serine/threonine-protein kinase component of the WNK2-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. The WNK2-SPAK/OSR1 kinase cascade is composed of WNK2, which mediates phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK. Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters, regulating their activity. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.
Uniprot ID
Q9Y3S1
Ensemble ID
ENST00000297954.9
HGNC ID
HGNC:14542
ChEMBL ID
CHEMBL5639

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0222  [1]
DBIA
 Probe Info  		C433(13.16); C435(13.16); C1153(1.04)  LDD0080  [2]
IA-alkyne
 Probe Info  		C2051(0.00); C1195(0.00); C326(0.00)  LDD0165  [3]
TFBX
 Probe Info  		N.A.  LDD0027  [4]
IPM
 Probe Info  		C79(0.00); C2051(0.00)  LDD0005  [5]
NPM
 Probe Info  		N.A.  LDD0016  [5]
AOyne
 Probe Info  		7.90  LDD0443  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C433(0.80); C435(0.98)  LDD0812  [2]
 LDCM0215  AC10 HCT 116 C433(1.12); C435(1.12)  LDD0532  [2]
 LDCM0216  AC100 HCT 116 C433(0.74); C435(0.74)  LDD0533  [2]
 LDCM0217  AC101 HCT 116 C433(0.79); C435(0.79)  LDD0534  [2]
 LDCM0218  AC102 HCT 116 C433(0.80); C435(0.80)  LDD0535  [2]
 LDCM0219  AC103 HCT 116 C433(0.96); C435(0.96)  LDD0536  [2]
 LDCM0220  AC104 HCT 116 C433(0.84); C435(0.84)  LDD0537  [2]
 LDCM0221  AC105 HCT 116 C433(0.84); C435(0.84)  LDD0538  [2]
 LDCM0222  AC106 HCT 116 C433(0.97); C435(0.97)  LDD0539  [2]
 LDCM0223  AC107 HCT 116 C433(0.33); C435(1.09)  LDD0540  [2]
 LDCM0224  AC108 HCT 116 C433(0.57); C435(0.76)  LDD0541  [2]
 LDCM0225  AC109 HCT 116 C433(0.48); C435(0.79)  LDD0542  [2]
 LDCM0226  AC11 HCT 116 C433(0.84); C435(0.84)  LDD0543  [2]
 LDCM0227  AC110 HCT 116 C433(0.51); C435(0.73)  LDD0544  [2]
 LDCM0228  AC111 HCT 116 C433(0.63); C435(0.79)  LDD0545  [2]
 LDCM0229  AC112 HCT 116 C433(0.36); C435(0.71)  LDD0546  [2]
 LDCM0230  AC113 HCT 116 C433(0.93); C435(0.93)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C433(1.08); C435(1.08)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C433(1.00); C435(1.00)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C433(0.95); C435(0.95)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C433(1.07); C435(1.07)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C433(1.03); C435(1.03)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C433(1.09); C435(1.09)  LDD0553  [2]
 LDCM0237  AC12 HCT 116 C433(0.86); C435(0.86)  LDD0554  [2]
 LDCM0238  AC120 HCT 116 C433(0.98); C435(0.98)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C433(1.14); C435(1.14)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C433(1.07); C435(1.07)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C433(1.10); C435(1.10)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C433(1.14); C435(1.14)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C433(0.95); C435(0.95)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C433(1.16); C435(1.16)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C433(1.13); C435(1.13)  LDD0562  [2]
 LDCM0246  AC128 HCT 116 C433(1.04); C435(1.04)  LDD0563  [2]
 LDCM0247  AC129 HCT 116 C433(0.92); C435(0.92)  LDD0564  [2]
 LDCM0249  AC130 HCT 116 C433(0.83); C435(0.83)  LDD0566  [2]
 LDCM0250  AC131 HCT 116 C433(0.88); C435(0.88)  LDD0567  [2]
 LDCM0251  AC132 HCT 116 C433(0.94); C435(0.94)  LDD0568  [2]
 LDCM0252  AC133 HCT 116 C433(0.88); C435(0.88)  LDD0569  [2]
 LDCM0253  AC134 HCT 116 C433(0.95); C435(0.95)  LDD0570  [2]
 LDCM0254  AC135 HCT 116 C433(0.96); C435(0.96)  LDD0571  [2]
 LDCM0255  AC136 HCT 116 C433(0.94); C435(0.94)  LDD0572  [2]
 LDCM0256  AC137 HCT 116 C433(1.10); C435(1.10)  LDD0573  [2]
 LDCM0257  AC138 HCT 116 C433(1.27); C435(1.27)  LDD0574  [2]
 LDCM0258  AC139 HCT 116 C433(0.96); C435(0.96)  LDD0575  [2]
 LDCM0259  AC14 HCT 116 C433(0.91); C435(0.91)  LDD0576  [2]
 LDCM0260  AC140 HCT 116 C433(1.14); C435(1.14)  LDD0577  [2]
 LDCM0261  AC141 HCT 116 C433(1.18); C435(1.18)  LDD0578  [2]
 LDCM0262  AC142 HCT 116 C433(1.00); C435(1.00)  LDD0579  [2]
 LDCM0263  AC143 HCT 116 C433(1.08); C435(1.08)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C433(1.06); C435(1.06)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C433(1.16); C435(1.16)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C433(1.12); C435(1.12)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C433(1.15); C435(1.15)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C433(1.36); C435(1.36)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C433(1.19); C435(1.19)  LDD0586  [2]
 LDCM0270  AC15 HCT 116 C433(0.94); C435(0.94)  LDD0587  [2]
 LDCM0271  AC150 HCT 116 C433(1.07); C435(1.07)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C433(1.05); C435(1.05)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C433(1.18); C435(1.18)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C433(1.26); C435(1.26)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C433(1.20); C435(1.20)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C433(1.15); C435(1.15)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C433(1.09); C435(1.09)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C433(1.15); C435(1.15)  LDD2161  [2]
 LDCM0276  AC17 HCT 116 C433(1.14); C435(1.14)  LDD0593  [2]
 LDCM0277  AC18 HCT 116 C433(1.08); C435(1.08)  LDD0594  [2]
 LDCM0278  AC19 HCT 116 C433(0.99); C435(0.99)  LDD0595  [2]
 LDCM0279  AC2 HEK-293T C433(0.81); C435(0.68)  LDD0877  [2]
 LDCM0280  AC20 HCT 116 C433(1.16); C435(1.16)  LDD0597  [2]
 LDCM0281  AC21 HCT 116 C433(1.13); C435(1.13)  LDD0598  [2]
 LDCM0282  AC22 HCT 116 C433(0.97); C435(0.97)  LDD0599  [2]
 LDCM0283  AC23 HCT 116 C433(1.00); C435(1.00)  LDD0600  [2]
 LDCM0284  AC24 HCT 116 C433(1.06); C435(1.06)  LDD0601  [2]
 LDCM0285  AC25 HCT 116 C433(0.82); C435(0.82)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C433(0.87); C435(0.87)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C433(0.97); C435(0.97)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C433(0.91); C435(0.91)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C433(1.01); C435(1.01)  LDD0606  [2]
 LDCM0290  AC3 HEK-293T C433(0.85); C435(1.09)  LDD0888  [2]
 LDCM0291  AC30 HCT 116 C433(0.95); C435(0.95)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C433(0.87); C435(0.87)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C433(1.00); C435(1.00)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C433(1.03); C435(1.03)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C433(1.04); C435(1.04)  LDD0612  [2]
 LDCM0296  AC35 HCT 116 C433(0.94); C435(0.94)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C433(0.90); C435(0.90)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C433(0.87); C435(0.87)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C433(0.94); C435(0.94)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C433(1.05); C435(1.05)  LDD0617  [2]
 LDCM0301  AC4 HEK-293T C433(0.89); C435(1.01)  LDD0899  [2]
 LDCM0302  AC40 HCT 116 C433(0.99); C435(0.99)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C433(0.89); C435(0.89)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C433(1.03); C435(1.03)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C433(1.03); C435(1.03)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C433(0.98); C435(0.98)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C433(1.03); C435(1.03)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C433(0.93); C435(0.93)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C433(1.00); C435(1.00)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C433(1.06); C435(1.06)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C433(1.12); C435(1.12)  LDD0628  [2]
 LDCM0312  AC5 HEK-293T C433(1.06); C435(0.99)  LDD0910  [2]
 LDCM0313  AC50 HCT 116 C433(1.04); C435(1.04)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C433(0.96); C435(0.96)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C433(0.98); C435(0.98)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C433(1.04); C435(1.04)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C433(1.33); C435(1.33)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C433(1.12); C435(1.12)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C433(1.12); C435(1.12)  LDD0636  [2]
 LDCM0320  AC57 HCT 116 C433(1.13); C435(1.13)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C433(0.99); C435(0.99)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C433(1.07); C435(1.07)  LDD0639  [2]
 LDCM0323  AC6 HCT 116 C433(1.00); C435(1.00)  LDD0640  [2]
 LDCM0324  AC60 HCT 116 C433(0.86); C435(0.86)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C433(0.91); C435(0.91)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C433(0.96); C435(0.96)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C433(0.89); C435(0.89)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C433(1.05); C435(1.05)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C433(1.05); C435(1.05)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C433(1.02); C435(1.02)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C433(1.05); C435(1.05)  LDD0648  [2]
 LDCM0332  AC68 HCT 116 C433(1.09); C435(1.09)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C433(1.13); C435(1.13)  LDD0650  [2]
 LDCM0334  AC7 HCT 116 C433(0.95); C435(0.95)  LDD0651  [2]
 LDCM0335  AC70 HCT 116 C433(1.14); C435(1.14)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C433(1.02); C435(1.02)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C433(1.03); C435(1.03)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C433(1.15); C435(1.15)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C433(1.21); C435(1.21)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C433(1.28); C435(1.28)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C433(1.04); C435(1.04)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C433(1.07); C435(1.07)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C433(0.95); C435(0.95)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C433(1.00); C435(1.00)  LDD0661  [2]
 LDCM0345  AC8 HCT 116 C433(0.83); C435(0.83)  LDD0662  [2]
 LDCM0346  AC80 HCT 116 C433(1.02); C435(1.02)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C433(0.94); C435(0.94)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C433(1.27); C435(1.27)  LDD0665  [2]
 LDCM0349  AC83 HCT 116 C433(1.21); C435(1.21)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C433(1.17); C435(1.17)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C433(1.00); C435(1.00)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C433(1.09); C435(1.09)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C433(1.01); C435(1.01)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C433(1.13); C435(1.13)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C433(1.19); C435(1.19)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C433(1.13); C435(1.13)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C433(1.23); C435(1.23)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C433(1.17); C435(1.17)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C433(1.02); C435(1.02)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C433(1.15); C435(1.15)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C433(1.01); C435(1.01)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C433(1.21); C435(1.21)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C433(1.15); C435(1.15)  LDD0681  [2]
 LDCM0365  AC98 HCT 116 C433(1.09); C435(1.09)  LDD0682  [2]
 LDCM0366  AC99 HCT 116 C433(0.78); C435(0.78)  LDD0683  [2]
 LDCM0248  AKOS034007472 HCT 116 C433(1.04); C435(1.04)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C433(0.94); C435(0.94)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C433(0.85); C435(0.85)  LDD0592  [2]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [1]
 LDCM0367  CL1 HCT 116 C433(0.90); C435(0.90)  LDD0684  [2]
 LDCM0368  CL10 HCT 116 C433(1.04); C435(1.04)  LDD0685  [2]
 LDCM0369  CL100 HEK-293T C433(0.88); C435(0.87)  LDD0967  [2]
 LDCM0370  CL101 HCT 116 C433(0.96); C435(0.96)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C433(0.87); C435(0.87)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C433(0.83); C435(0.83)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C433(0.80); C435(0.80)  LDD0690  [2]
 LDCM0374  CL105 HCT 116 C433(1.15); C435(1.15)  LDD0691  [2]
 LDCM0375  CL106 HCT 116 C433(1.16); C435(1.16)  LDD0692  [2]
 LDCM0376  CL107 HCT 116 C433(1.12); C435(1.12)  LDD0693  [2]
 LDCM0377  CL108 HCT 116 C433(1.17); C435(1.17)  LDD0694  [2]
 LDCM0378  CL109 HCT 116 C433(0.99); C435(0.99)  LDD0695  [2]
 LDCM0379  CL11 HCT 116 C433(0.98); C435(0.98)  LDD0696  [2]
 LDCM0380  CL110 HCT 116 C433(1.02); C435(1.02)  LDD0697  [2]
 LDCM0381  CL111 HCT 116 C433(0.92); C435(0.92)  LDD0698  [2]
 LDCM0382  CL112 HCT 116 C433(0.75); C435(0.75)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C433(0.92); C435(0.92)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C433(0.82); C435(0.82)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C433(0.90); C435(0.90)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C433(0.87); C435(0.87)  LDD0703  [2]
 LDCM0387  CL117 HCT 116 C433(1.28); C435(1.28)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C433(0.96); C435(0.96)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C433(1.01); C435(1.01)  LDD0706  [2]
 LDCM0390  CL12 HCT 116 C433(1.11); C435(1.11)  LDD0707  [2]
 LDCM0391  CL120 HCT 116 C433(0.95); C435(0.95)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C433(1.29); C435(1.29)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C433(1.01); C435(1.01)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C433(1.13); C435(1.13)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C433(1.16); C435(1.16)  LDD0712  [2]
 LDCM0396  CL125 HCT 116 C433(0.85); C435(0.85)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C433(0.77); C435(0.77)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C433(0.82); C435(0.82)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C433(1.01); C435(1.01)  LDD0716  [2]
 LDCM0400  CL13 HCT 116 C433(1.10); C435(1.10)  LDD0717  [2]
 LDCM0401  CL14 HCT 116 C433(1.10); C435(1.10)  LDD0718  [2]
 LDCM0402  CL15 HCT 116 C433(1.06); C435(1.06)  LDD0719  [2]
 LDCM0403  CL16 HCT 116 C433(0.96); C435(0.96)  LDD0720  [2]
 LDCM0404  CL17 HEK-293T C433(0.87); C435(0.92)  LDD1002  [2]
 LDCM0405  CL18 HEK-293T C433(1.12); C435(1.08)  LDD1003  [2]
 LDCM0406  CL19 HEK-293T C433(1.00); C435(1.07)  LDD1004  [2]
 LDCM0407  CL2 HCT 116 C433(0.98); C435(0.98)  LDD0724  [2]
 LDCM0408  CL20 HEK-293T C433(1.04); C435(1.08)  LDD1006  [2]
 LDCM0409  CL21 HEK-293T C433(1.31); C435(1.19)  LDD1007  [2]
 LDCM0410  CL22 HEK-293T C433(0.99); C435(1.05)  LDD1008  [2]
 LDCM0411  CL23 HEK-293T C433(1.04); C435(1.08)  LDD1009  [2]
 LDCM0412  CL24 HEK-293T C433(1.32); C435(1.20)  LDD1010  [2]
 LDCM0413  CL25 HEK-293T C433(1.06); C435(1.10)  LDD1011  [2]
 LDCM0414  CL26 HEK-293T C433(0.99); C435(1.14)  LDD1012  [2]
 LDCM0415  CL27 HEK-293T C433(1.21); C435(1.13)  LDD1013  [2]
 LDCM0416  CL28 HEK-293T C433(1.00); C435(1.05)  LDD1014  [2]
 LDCM0417  CL29 HEK-293T C433(1.08); C435(1.05)  LDD1015  [2]
 LDCM0418  CL3 HCT 116 C433(1.00); C435(1.00)  LDD0735  [2]
 LDCM0419  CL30 HEK-293T C433(0.99); C435(1.02)  LDD1017  [2]
 LDCM0420  CL31 HEK-293T C433(1.00); C435(0.97)  LDD1018  [2]
 LDCM0421  CL32 HCT 116 C433(0.98); C435(0.98)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C433(0.98); C435(0.98)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C433(0.91); C435(0.91)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C433(0.97); C435(0.97)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C433(1.09); C435(1.09)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C433(1.06); C435(1.06)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C433(1.24); C435(1.24)  LDD0745  [2]
 LDCM0429  CL4 HCT 116 C433(0.93); C435(0.93)  LDD0746  [2]
 LDCM0430  CL40 HCT 116 C433(0.97); C435(0.97)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C433(0.87); C435(0.87)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C433(0.99); C435(0.99)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C433(0.87); C435(0.87)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C433(0.84); C435(0.84)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C433(0.85); C435(0.85)  LDD0752  [2]
 LDCM0436  CL46 HEK-293T C433(0.97); C435(1.09)  LDD1034  [2]
 LDCM0437  CL47 HEK-293T C433(0.92); C435(1.08)  LDD1035  [2]
 LDCM0438  CL48 HEK-293T C433(0.86); C435(1.07)  LDD1036  [2]
 LDCM0439  CL49 HEK-293T C433(0.91); C435(1.04)  LDD1037  [2]
 LDCM0440  CL5 HCT 116 C433(1.06); C435(1.06)  LDD0757  [2]
 LDCM0441  CL50 HEK-293T C433(1.10); C435(1.23)  LDD1039  [2]
 LDCM0442  CL51 HEK-293T C433(0.94); C435(1.19)  LDD1040  [2]
 LDCM0443  CL52 HEK-293T C433(0.99); C435(1.16)  LDD1041  [2]
 LDCM0444  CL53 HEK-293T C433(0.85); C435(1.10)  LDD1042  [2]
 LDCM0445  CL54 HEK-293T C433(0.90); C435(1.16)  LDD1043  [2]
 LDCM0446  CL55 HEK-293T C433(0.88); C435(1.04)  LDD1044  [2]
 LDCM0447  CL56 HEK-293T C433(0.92); C435(1.10)  LDD1045  [2]
 LDCM0448  CL57 HEK-293T C433(0.95); C435(1.17)  LDD1046  [2]
 LDCM0449  CL58 HEK-293T C433(0.95); C435(1.10)  LDD1047  [2]
 LDCM0450  CL59 HEK-293T C433(0.94); C435(1.05)  LDD1048  [2]
 LDCM0451  CL6 HCT 116 C433(0.97); C435(0.97)  LDD0768  [2]
 LDCM0452  CL60 HEK-293T C433(0.99); C435(1.11)  LDD1050  [2]
 LDCM0453  CL61 HCT 116 C433(0.76); C435(0.76)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C433(0.75); C435(0.75)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C433(0.74); C435(0.74)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C433(0.77); C435(0.77)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C433(0.87); C435(0.87)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C433(0.72); C435(0.72)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C433(0.83); C435(0.83)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C433(0.79); C435(0.79)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C433(0.80); C435(0.80)  LDD0778  [2]
 LDCM0462  CL7 HCT 116 C433(0.85); C435(0.85)  LDD0779  [2]
 LDCM0463  CL70 HCT 116 C433(0.84); C435(0.84)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C433(0.77); C435(0.77)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C433(0.83); C435(0.83)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C433(0.79); C435(0.79)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C433(0.83); C435(0.83)  LDD0784  [2]
 LDCM0469  CL76 HCT 116 C433(0.97); C435(0.97)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C433(0.95); C435(0.95)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C433(1.22); C435(1.22)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C433(1.11); C435(1.11)  LDD0789  [2]
 LDCM0473  CL8 HCT 116 C433(1.01); C435(1.01)  LDD0790  [2]
 LDCM0474  CL80 HCT 116 C433(1.05); C435(1.05)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C433(1.28); C435(1.28)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C433(1.20); C435(1.20)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C433(1.03); C435(1.03)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C433(1.30); C435(1.30)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C433(1.09); C435(1.09)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C433(1.04); C435(1.04)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C433(1.07); C435(1.07)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C433(1.17); C435(1.17)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C433(1.41); C435(1.41)  LDD0800  [2]
 LDCM0484  CL9 HCT 116 C433(0.94); C435(0.94)  LDD0801  [2]
 LDCM0485  CL90 HCT 116 C433(0.88); C435(0.88)  LDD0802  [2]
 LDCM0486  CL91 HEK-293T C433(1.07); C435(1.30)  LDD1084  [2]
 LDCM0487  CL92 HEK-293T C433(0.81); C435(1.03)  LDD1085  [2]
 LDCM0488  CL93 HEK-293T C433(0.86); C435(1.01)  LDD1086  [2]
 LDCM0489  CL94 HEK-293T C433(1.03); C435(0.94)  LDD1087  [2]
 LDCM0490  CL95 HEK-293T C433(0.96); C435(0.94)  LDD1088  [2]
 LDCM0491  CL96 HEK-293T C433(0.94); C435(1.03)  LDD1089  [2]
 LDCM0492  CL97 HEK-293T C433(1.01); C435(0.97)  LDD1090  [2]
 LDCM0493  CL98 HEK-293T C433(0.92); C435(1.06)  LDD1091  [2]
 LDCM0494  CL99 HEK-293T C433(0.97); C435(1.18)  LDD1092  [2]
 LDCM0625  F8 Ramos C2051(1.71)  LDD2187  [7]
 LDCM0573  Fragment11 Ramos C2051(0.69)  LDD2190  [7]
 LDCM0574  Fragment12 Ramos C2051(1.65)  LDD2191  [7]
 LDCM0575  Fragment13 Ramos C2051(1.03)  LDD2192  [7]
 LDCM0576  Fragment14 Ramos C2051(0.74)  LDD2193  [7]
 LDCM0579  Fragment20 Ramos C2051(1.57)  LDD2194  [7]
 LDCM0580  Fragment21 Ramos C2051(1.14)  LDD2195  [7]
 LDCM0582  Fragment23 Ramos C2051(1.31)  LDD2196  [7]
 LDCM0578  Fragment27 Ramos C2051(0.93)  LDD2197  [7]
 LDCM0586  Fragment28 Ramos C2051(0.92)  LDD2198  [7]
 LDCM0588  Fragment30 Ramos C2051(1.19)  LDD2199  [7]
 LDCM0589  Fragment31 Ramos C2051(1.24)  LDD2200  [7]
 LDCM0590  Fragment32 Ramos C2051(2.65)  LDD2201  [7]
 LDCM0468  Fragment33 HCT 116 C433(0.70); C435(0.70)  LDD0785  [2]
 LDCM0596  Fragment38 Ramos C2051(1.12)  LDD2203  [7]
 LDCM0566  Fragment4 Ramos C2051(1.01)  LDD2184  [7]
 LDCM0427  Fragment51 HCT 116 C433(1.01); C435(1.01)  LDD0744  [2]
 LDCM0610  Fragment52 Ramos C2051(1.65)  LDD2204  [7]
 LDCM0614  Fragment56 Ramos C2051(1.67)  LDD2205  [7]
 LDCM0569  Fragment7 Ramos C2051(1.55)  LDD2186  [7]
 LDCM0571  Fragment9 Ramos C2051(2.85)  LDD2188  [7]
 LDCM0022  KB02 HCT 116 C433(13.16); C435(13.16); C1153(1.04)  LDD0080  [2]
 LDCM0023  KB03 HCT 116 C433(10.29); C435(10.29); C1153(0.98)  LDD0081  [2]
 LDCM0024  KB05 HCT 116 C433(11.30); C435(11.30); C1153(0.96)  LDD0082  [2]

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
7 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578