Details of the Target

General Information of Target

Target ID LDTP04107
Target Name Large ribosomal subunit protein eL21 (RPL21)
Gene Name RPL21
Gene ID 6144
Synonyms
Large ribosomal subunit protein eL21; 60S ribosomal protein L21
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGK
TGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAK
EKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA
Target Bioclass
Other
Family
Eukaryotic ribosomal protein eL21 family
Subcellular location
Cytoplasm, cytosol
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Uniprot ID
P46778
Ensemble ID
ENST00000272274.8
HGNC ID
HGNC:10313

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 32 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		14.39  LDD0402  [1]
A-EBA
 Probe Info  		2.48  LDD0215  [2]
C-Sul
 Probe Info  		3.24  LDD0066  [3]
TH211
 Probe Info  		Y65(20.00)  LDD0257  [4]
TH214
 Probe Info  		Y65(11.48)  LDD0258  [4]
TH216
 Probe Info  		Y30(20.00); Y65(20.00)  LDD0259  [4]
YN-1
 Probe Info  		100.00  LDD0444  [5]
ONAyne
 Probe Info  		K36(1.00)  LDD0274  [6]
STPyne
 Probe Info  		K122(5.53); K21(1.21); K36(0.85); K97(10.00)  LDD0277  [6]
BTD
 Probe Info  		C56(1.06)  LDD2117  [7]
EA-probe
 Probe Info  		N.A.  LDD0440  [8]
HHS-482
 Probe Info  		Y156(1.24); Y30(0.87); Y65(0.85)  LDD0285  [9]
HHS-465
 Probe Info  		Y156(0.73); Y65(10.00)  LDD2237  [10]
DBIA
 Probe Info  		C56(1.04)  LDD1510  [11]
ATP probe
 Probe Info  		K122(0.00); K78(0.00); K81(0.00); K36(0.00)  LDD0199  [12]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [13]
ATP probe
 Probe Info  		K43(0.00); K78(0.00)  LDD0035  [14]
IPM
 Probe Info  		N.A.  LDD0147  [15]
NHS
 Probe Info  		N.A.  LDD0010  [16]
OSF
 Probe Info  		H70(0.00); Y65(0.00)  LDD0029  [17]
SF
 Probe Info  		K78(0.00); K97(0.00); Y156(0.00); Y30(0.00)  LDD0028  [17]
TFBX
 Probe Info  		N.A.  LDD0148  [15]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [18]
Ox-W18
 Probe Info  		N.A.  LDD2175  [19]
1c-yne
 Probe Info  		K43(0.00); K21(0.00)  LDD0228  [20]
1d-yne
 Probe Info  		N.A.  LDD0357  [20]
Acrolein
 Probe Info  		H70(0.00); H139(0.00)  LDD0217  [21]
Crotonaldehyde
 Probe Info  		H70(0.00); H98(0.00); H139(0.00)  LDD0219  [21]
Methacrolein
 Probe Info  		N.A.  LDD0218  [21]
AOyne
 Probe Info  		12.90  LDD0443  [22]
NAIA_5
 Probe Info  		N.A.  LDD2223  [23]
HHS-475
 Probe Info  		Y65(1.22)  LDD2238  [10]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0137  [24]
STS-2
 Probe Info  		N.A.  LDD0138  [24]
Dasatinib-CA-3PAP
 Probe Info  		6.50  LDD0365  [25]
DA-2
 Probe Info  		N.A.  LDD0071  [26]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C56(1.06)  LDD2117  [7]
 LDCM0237  AC12 HEK-293T C56(1.04)  LDD1510  [11]
 LDCM0280  AC20 HEK-293T C56(1.09)  LDD1519  [11]
 LDCM0288  AC28 HEK-293T C56(1.02)  LDD1527  [11]
 LDCM0297  AC36 HEK-293T C56(1.12)  LDD1536  [11]
 LDCM0301  AC4 HEK-293T C56(1.11)  LDD1540  [11]
 LDCM0306  AC44 HEK-293T C56(1.07)  LDD1545  [11]
 LDCM0315  AC52 HEK-293T C56(1.00)  LDD1554  [11]
 LDCM0324  AC60 HEK-293T C56(0.92)  LDD1563  [11]
 LDCM0156  Aniline NCI-H1299 12.30  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa H70(0.00); H98(0.00); H139(0.00)  LDD0222  [21]
 LDCM0632  CL-Sc Hep-G2 C56(0.42)  LDD2227  [23]
 LDCM0372  CL103 HEK-293T C56(1.19)  LDD1576  [11]
 LDCM0376  CL107 HEK-293T C56(1.06)  LDD1580  [11]
 LDCM0381  CL111 HEK-293T C56(1.11)  LDD1585  [11]
 LDCM0385  CL115 HEK-293T C56(1.13)  LDD1589  [11]
 LDCM0389  CL119 HEK-293T C56(1.09)  LDD1593  [11]
 LDCM0394  CL123 HEK-293T C56(1.10)  LDD1598  [11]
 LDCM0398  CL127 HEK-293T C56(0.87)  LDD1602  [11]
 LDCM0402  CL15 HEK-293T C56(1.05)  LDD1606  [11]
 LDCM0408  CL20 HEK-293T C56(0.69)  LDD1612  [11]
 LDCM0415  CL27 HEK-293T C56(1.06)  LDD1619  [11]
 LDCM0418  CL3 HEK-293T C56(0.87)  LDD1622  [11]
 LDCM0421  CL32 HEK-293T C56(0.70)  LDD1625  [11]
 LDCM0428  CL39 HEK-293T C56(1.25)  LDD1632  [11]
 LDCM0434  CL44 HEK-293T C56(0.74)  LDD1638  [11]
 LDCM0447  CL56 HEK-293T C56(0.80)  LDD1650  [11]
 LDCM0455  CL63 HEK-293T C56(1.09)  LDD1658  [11]
 LDCM0460  CL68 HEK-293T C56(0.68)  LDD1663  [11]
 LDCM0473  CL8 HEK-293T C56(0.65)  LDD1676  [11]
 LDCM0474  CL80 HEK-293T C56(0.72)  LDD1677  [11]
 LDCM0481  CL87 HEK-293T C56(0.96)  LDD1684  [11]
 LDCM0487  CL92 HEK-293T C56(0.79)  LDD1690  [11]
 LDCM0494  CL99 HEK-293T C56(0.99)  LDD1697  [11]
 LDCM0097  Dasatinib K562 6.50  LDD0365  [25]
 LDCM0495  E2913 HEK-293T C56(1.25)  LDD1698  [11]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [8]
 LDCM0573  Fragment11 Ramos C56(20.00)  LDD2190  [27]
 LDCM0576  Fragment14 Ramos C56(0.29)  LDD2193  [27]
 LDCM0586  Fragment28 Ramos C56(0.69)  LDD2198  [27]
 LDCM0468  Fragment33 HEK-293T C56(1.18)  LDD1671  [11]
 LDCM0566  Fragment4 Ramos C56(0.64)  LDD2184  [27]
 LDCM0569  Fragment7 Ramos C56(0.43)  LDD2186  [27]
 LDCM0107  IAA HeLa H70(0.00); H98(0.00); H139(0.00)  LDD0221  [21]
 LDCM0123  JWB131 DM93 Y156(1.24); Y30(0.87); Y65(0.85)  LDD0285  [9]
 LDCM0124  JWB142 DM93 Y156(0.76); Y30(0.47); Y65(0.73)  LDD0286  [9]
 LDCM0125  JWB146 DM93 Y156(1.03); Y30(0.53); Y65(0.93)  LDD0287  [9]
 LDCM0126  JWB150 DM93 Y156(2.61); Y30(1.13); Y65(2.95)  LDD0288  [9]
 LDCM0127  JWB152 DM93 Y156(1.24); Y30(0.81); Y65(1.91)  LDD0289  [9]
 LDCM0128  JWB198 DM93 Y156(1.05); Y30(0.47); Y65(1.34)  LDD0290  [9]
 LDCM0129  JWB202 DM93 Y156(0.44); Y30(0.28); Y65(0.44)  LDD0291  [9]
 LDCM0130  JWB211 DM93 Y156(1.42); Y30(0.45); Y65(0.87)  LDD0292  [9]
 LDCM0022  KB02 Ramos C56(0.54)  LDD2182  [27]
 LDCM0023  KB03 Ramos C56(0.62)  LDD2183  [27]
 LDCM0024  KB05 Ramos C56(0.38)  LDD2185  [27]
 LDCM0109  NEM HeLa H70(0.00); H139(0.00); H98(0.00)  LDD0223  [21]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
6 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
7 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
8 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
9 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
10 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
12 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
13 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
14 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
15 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
16 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
17 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
18 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
19 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
20 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
21 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
22 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
23 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
24 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
25 Streamlined Target Deconvolution Approach Utilizing a Single Photoreactive Chloroalkane Capture Tag. ACS Chem Biol. 2021 Feb 19;16(2):404-413. doi: 10.1021/acschembio.0c00987. Epub 2021 Feb 5.
26 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
27 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578