Details of the Target

General Information of Target

Target ID LDTP13349
Target Name E3 ISG15--protein ligase HERC5 (HERC5)
Gene Name HERC5
Gene ID 51191
Synonyms
CEB1; CEBP1; E3 ISG15--protein ligase HERC5; EC 2.3.2.-; Cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MAVTALAARTWLGVWGVRTMQARGFGSDQSENVDRGAGSIREAGGAFGKREQAEEERYFR
AQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLKHDD
Target Bioclass
Enzyme
Subcellular location
Cytoplasm, perinuclear region
Function
Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Mediates ISGylation of the phosphatase PTEN leading to its degradation, thus alleviating its suppression of the PI3K-AKT signaling pathway and promoting the production of cytokines that facilitate bacterial clearance. Interferes with the function of key viral structural proteins such as ebolavirus structural protein VP40 or HIV-1 protein GAG. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15. Promotes parkin/PRKN ubiquitin E3 ligase activity by suppressing the intramolecular interaction that maintains its autoinhibited conformation.; (Microbial infection) Functions as an E3 ligase for ISGylation of hepatitis B virus protein X leading to enhanced viral replication due to increased interferon resistance.
Uniprot ID
Q9UII4
Ensemble ID
ENST00000264350.8
HGNC ID
HGNC:24368

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K643(5.26)  LDD0277  [1]
Johansson_61
 Probe Info  		_(4.06)  LDD1490  [2]
AHL-Pu-1
 Probe Info  		C994(2.04)  LDD0168  [3]
DBIA
 Probe Info  		C608(31.49); C302(10.46)  LDD0209  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C994(0.00); C424(0.00); C302(0.00); C585(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		C468(0.00); C994(0.00)  LDD0036  [5]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [6]
Lodoacetamide azide
 Probe Info  		C994(0.00); C468(0.00); C424(0.00); C145(0.00)  LDD0037  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [7]
IPM
 Probe Info  		N.A.  LDD0147  [8]
YN-1
 Probe Info  		N.A.  LDD0447  [9]
AOyne
 Probe Info  		15.00  LDD0443  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C994(2.04)  LDD0168  [3]
 LDCM0026  4SU-RNA+native RNA DM93 C994(20.00)  LDD0171  [3]
 LDCM0214  AC1 HEK-293T C994(1.01)  LDD0812  [11]
 LDCM0215  AC10 HCT 116 C608(1.71)  LDD0532  [11]
 LDCM0216  AC100 HCT 116 C220(1.06); C302(0.64)  LDD0533  [11]
 LDCM0217  AC101 HCT 116 C220(1.19); C302(0.75)  LDD0534  [11]
 LDCM0218  AC102 HCT 116 C220(0.86); C302(0.59)  LDD0535  [11]
 LDCM0219  AC103 HCT 116 C220(0.88); C302(0.48)  LDD0536  [11]
 LDCM0220  AC104 HCT 116 C220(0.92); C302(0.48)  LDD0537  [11]
 LDCM0221  AC105 HCT 116 C220(1.16); C302(0.51)  LDD0538  [11]
 LDCM0222  AC106 HCT 116 C220(0.96); C302(0.60)  LDD0539  [11]
 LDCM0223  AC107 HCT 116 C220(1.20); C302(0.75)  LDD0540  [11]
 LDCM0224  AC108 HCT 116 C220(0.75); C302(0.52)  LDD0541  [11]
 LDCM0225  AC109 HCT 116 C220(0.79); C302(0.49)  LDD0542  [11]
 LDCM0226  AC11 HCT 116 C608(1.58)  LDD0543  [11]
 LDCM0227  AC110 HCT 116 C220(0.77); C302(0.55)  LDD0544  [11]
 LDCM0228  AC111 HCT 116 C220(0.92); C302(0.57)  LDD0545  [11]
 LDCM0229  AC112 HCT 116 C220(0.85); C302(0.55)  LDD0546  [11]
 LDCM0230  AC113 HCT 116 C302(0.94)  LDD0547  [11]
 LDCM0231  AC114 HCT 116 C302(0.62)  LDD0548  [11]
 LDCM0232  AC115 HCT 116 C302(0.51)  LDD0549  [11]
 LDCM0233  AC116 HCT 116 C302(0.52)  LDD0550  [11]
 LDCM0234  AC117 HCT 116 C302(0.78)  LDD0551  [11]
 LDCM0235  AC118 HCT 116 C302(0.91)  LDD0552  [11]
 LDCM0236  AC119 HCT 116 C302(0.70)  LDD0553  [11]
 LDCM0237  AC12 HCT 116 C608(1.24)  LDD0554  [11]
 LDCM0238  AC120 HCT 116 C302(0.78)  LDD0555  [11]
 LDCM0239  AC121 HCT 116 C302(0.88)  LDD0556  [11]
 LDCM0240  AC122 HCT 116 C302(1.04)  LDD0557  [11]
 LDCM0241  AC123 HCT 116 C302(0.73)  LDD0558  [11]
 LDCM0242  AC124 HCT 116 C302(1.21)  LDD0559  [11]
 LDCM0243  AC125 HCT 116 C302(0.76)  LDD0560  [11]
 LDCM0244  AC126 HCT 116 C302(0.79)  LDD0561  [11]
 LDCM0245  AC127 HCT 116 C302(0.56)  LDD0562  [11]
 LDCM0246  AC128 HEK-293T C994(0.97)  LDD0844  [11]
 LDCM0247  AC129 HEK-293T C994(1.17)  LDD0845  [11]
 LDCM0249  AC130 HEK-293T C994(1.49)  LDD0847  [11]
 LDCM0250  AC131 HEK-293T C994(0.91)  LDD0848  [11]
 LDCM0251  AC132 HEK-293T C994(1.27)  LDD0849  [11]
 LDCM0252  AC133 HEK-293T C994(1.55)  LDD0850  [11]
 LDCM0253  AC134 HEK-293T C994(1.33)  LDD0851  [11]
 LDCM0254  AC135 HEK-293T C994(1.12)  LDD0852  [11]
 LDCM0255  AC136 HEK-293T C994(1.31)  LDD0853  [11]
 LDCM0256  AC137 HEK-293T C994(1.50)  LDD0854  [11]
 LDCM0257  AC138 HEK-293T C994(2.75)  LDD0855  [11]
 LDCM0258  AC139 HEK-293T C994(2.17)  LDD0856  [11]
 LDCM0259  AC14 HCT 116 C608(1.71)  LDD0576  [11]
 LDCM0260  AC140 HEK-293T C994(1.30)  LDD0858  [11]
 LDCM0261  AC141 HEK-293T C994(2.33)  LDD0859  [11]
 LDCM0262  AC142 HEK-293T C994(1.54)  LDD0860  [11]
 LDCM0263  AC143 HEK-293T C608(0.80)  LDD0861  [11]
 LDCM0264  AC144 HEK-293T C608(0.92)  LDD0862  [11]
 LDCM0265  AC145 HEK-293T C608(1.10)  LDD0863  [11]
 LDCM0266  AC146 HEK-293T C608(0.80)  LDD0864  [11]
 LDCM0267  AC147 HEK-293T C608(1.05)  LDD0865  [11]
 LDCM0268  AC148 HEK-293T C608(1.17)  LDD0866  [11]
 LDCM0269  AC149 HEK-293T C608(0.88)  LDD0867  [11]
 LDCM0270  AC15 HCT 116 C608(1.57)  LDD0587  [11]
 LDCM0271  AC150 HEK-293T C608(1.31)  LDD0869  [11]
 LDCM0272  AC151 HEK-293T C608(0.82)  LDD0870  [11]
 LDCM0273  AC152 HEK-293T C608(0.96)  LDD0871  [11]
 LDCM0274  AC153 HEK-293T C608(1.19)  LDD0872  [11]
 LDCM0621  AC154 HEK-293T C608(0.87)  LDD2162  [11]
 LDCM0622  AC155 HEK-293T C608(0.95)  LDD2163  [11]
 LDCM0623  AC156 HEK-293T C608(0.83)  LDD2164  [11]
 LDCM0624  AC157 HEK-293T C608(1.47)  LDD2165  [11]
 LDCM0276  AC17 HCT 116 C220(0.75)  LDD0593  [11]
 LDCM0277  AC18 HCT 116 C220(0.80)  LDD0594  [11]
 LDCM0278  AC19 HCT 116 C220(1.04)  LDD0595  [11]
 LDCM0279  AC2 HEK-293T C994(1.25)  LDD0877  [11]
 LDCM0280  AC20 HCT 116 C220(0.84)  LDD0597  [11]
 LDCM0281  AC21 HCT 116 C220(0.80)  LDD0598  [11]
 LDCM0282  AC22 HCT 116 C220(0.75)  LDD0599  [11]
 LDCM0283  AC23 HCT 116 C220(0.74)  LDD0600  [11]
 LDCM0284  AC24 HCT 116 C220(0.89)  LDD0601  [11]
 LDCM0285  AC25 HCT 116 C302(0.74); C608(0.94); C220(1.27)  LDD0602  [11]
 LDCM0286  AC26 HCT 116 C302(0.62); C220(1.27); C608(1.32)  LDD0603  [11]
 LDCM0287  AC27 HCT 116 C302(0.50); C220(1.16); C608(1.32)  LDD0604  [11]
 LDCM0288  AC28 HCT 116 C302(0.57); C220(1.32); C608(1.40)  LDD0605  [11]
 LDCM0289  AC29 HCT 116 C302(0.69); C220(1.16); C608(1.26)  LDD0606  [11]
 LDCM0290  AC3 HEK-293T C994(0.93)  LDD0888  [11]
 LDCM0291  AC30 HCT 116 C302(0.56); C608(1.26); C220(1.67)  LDD0608  [11]
 LDCM0292  AC31 HCT 116 C302(0.55); C220(1.19); C608(1.40)  LDD0609  [11]
 LDCM0293  AC32 HCT 116 C302(0.38); C220(1.40); C608(1.46)  LDD0610  [11]
 LDCM0294  AC33 HCT 116 C302(0.45); C608(0.98); C220(1.16)  LDD0611  [11]
 LDCM0295  AC34 HCT 116 C302(0.36); C608(1.05); C220(1.36)  LDD0612  [11]
 LDCM0296  AC35 HEK-293T C994(1.03)  LDD0894  [11]
 LDCM0297  AC36 HEK-293T C994(0.99)  LDD0895  [11]
 LDCM0298  AC37 HEK-293T C994(0.91)  LDD0896  [11]
 LDCM0299  AC38 HEK-293T C994(0.99)  LDD0897  [11]
 LDCM0300  AC39 HEK-293T C994(1.09)  LDD0898  [11]
 LDCM0301  AC4 HEK-293T C994(0.90)  LDD0899  [11]
 LDCM0302  AC40 HEK-293T C994(1.00)  LDD0900  [11]
 LDCM0303  AC41 HEK-293T C994(1.04)  LDD0901  [11]
 LDCM0304  AC42 HEK-293T C994(1.03)  LDD0902  [11]
 LDCM0305  AC43 HEK-293T C994(0.81)  LDD0903  [11]
 LDCM0306  AC44 HEK-293T C994(0.98)  LDD0904  [11]
 LDCM0307  AC45 HEK-293T C994(0.99)  LDD0905  [11]
 LDCM0308  AC46 HCT 116 C608(1.03)  LDD0625  [11]
 LDCM0309  AC47 HCT 116 C608(1.27)  LDD0626  [11]
 LDCM0310  AC48 HCT 116 C608(1.43)  LDD0627  [11]
 LDCM0311  AC49 HCT 116 C608(1.22)  LDD0628  [11]
 LDCM0312  AC5 HEK-293T C994(0.94)  LDD0910  [11]
 LDCM0313  AC50 HCT 116 C608(1.13)  LDD0630  [11]
 LDCM0314  AC51 HCT 116 C608(0.83)  LDD0631  [11]
 LDCM0315  AC52 HCT 116 C608(0.93)  LDD0632  [11]
 LDCM0316  AC53 HCT 116 C608(1.15)  LDD0633  [11]
 LDCM0317  AC54 HCT 116 C608(1.51)  LDD0634  [11]
 LDCM0318  AC55 HCT 116 C608(1.26)  LDD0635  [11]
 LDCM0319  AC56 HCT 116 C608(1.30)  LDD0636  [11]
 LDCM0320  AC57 HEK-293T C994(1.13); C302(1.22)  LDD0918  [11]
 LDCM0321  AC58 HEK-293T C994(0.83); C302(1.22)  LDD0919  [11]
 LDCM0322  AC59 HEK-293T C994(0.90); C302(1.18)  LDD0920  [11]
 LDCM0323  AC6 HCT 116 C608(1.51)  LDD0640  [11]
 LDCM0324  AC60 HEK-293T C994(1.49); C302(1.00)  LDD0922  [11]
 LDCM0325  AC61 HEK-293T C994(0.84); C302(1.26)  LDD0923  [11]
 LDCM0326  AC62 HEK-293T C994(1.02); C302(0.93)  LDD0924  [11]
 LDCM0327  AC63 HEK-293T C994(0.95); C302(1.26)  LDD0925  [11]
 LDCM0328  AC64 HEK-293T C994(0.78); C302(1.28)  LDD0926  [11]
 LDCM0329  AC65 HEK-293T C994(0.91); C302(1.62)  LDD0927  [11]
 LDCM0330  AC66 HEK-293T C994(0.94); C302(1.01)  LDD0928  [11]
 LDCM0331  AC67 HEK-293T C994(1.06); C302(1.03)  LDD0929  [11]
 LDCM0332  AC68 HCT 116 C608(1.31)  LDD0649  [11]
 LDCM0333  AC69 HCT 116 C608(1.35)  LDD0650  [11]
 LDCM0334  AC7 HCT 116 C608(1.19)  LDD0651  [11]
 LDCM0335  AC70 HCT 116 C608(1.59)  LDD0652  [11]
 LDCM0336  AC71 HCT 116 C608(1.19)  LDD0653  [11]
 LDCM0337  AC72 HCT 116 C608(1.41)  LDD0654  [11]
 LDCM0338  AC73 HCT 116 C608(1.66)  LDD0655  [11]
 LDCM0339  AC74 HCT 116 C608(1.83)  LDD0656  [11]
 LDCM0340  AC75 HCT 116 C608(1.70)  LDD0657  [11]
 LDCM0341  AC76 HCT 116 C608(1.61)  LDD0658  [11]
 LDCM0342  AC77 HCT 116 C608(1.52)  LDD0659  [11]
 LDCM0343  AC78 HCT 116 C608(1.11)  LDD0660  [11]
 LDCM0344  AC79 HCT 116 C608(1.40)  LDD0661  [11]
 LDCM0345  AC8 HCT 116 C608(1.33)  LDD0662  [11]
 LDCM0346  AC80 HCT 116 C608(1.56)  LDD0663  [11]
 LDCM0347  AC81 HCT 116 C608(0.84)  LDD0664  [11]
 LDCM0348  AC82 HCT 116 C608(1.97)  LDD0665  [11]
 LDCM0349  AC83 HCT 116 C302(0.79); C220(0.89)  LDD0666  [11]
 LDCM0350  AC84 HCT 116 C302(0.71); C220(0.92)  LDD0667  [11]
 LDCM0351  AC85 HCT 116 C302(0.83); C220(1.05)  LDD0668  [11]
 LDCM0352  AC86 HCT 116 C302(1.15); C220(1.16)  LDD0669  [11]
 LDCM0353  AC87 HCT 116 C220(1.29); C302(1.36)  LDD0670  [11]
 LDCM0354  AC88 HCT 116 C220(0.96); C302(1.08)  LDD0671  [11]
 LDCM0355  AC89 HCT 116 C302(0.66); C220(0.89)  LDD0672  [11]
 LDCM0357  AC90 HCT 116 C302(1.10); C220(1.15)  LDD0674  [11]
 LDCM0358  AC91 HCT 116 C302(0.65); C220(0.98)  LDD0675  [11]
 LDCM0359  AC92 HCT 116 C302(0.61); C220(0.86)  LDD0676  [11]
 LDCM0360  AC93 HCT 116 C302(0.89); C220(0.91)  LDD0677  [11]
 LDCM0361  AC94 HCT 116 C220(1.10); C302(1.11)  LDD0678  [11]
 LDCM0362  AC95 HCT 116 C220(1.12); C302(1.21)  LDD0679  [11]
 LDCM0363  AC96 HCT 116 C220(0.89); C302(0.90)  LDD0680  [11]
 LDCM0364  AC97 HCT 116 C302(0.73); C220(0.91)  LDD0681  [11]
 LDCM0365  AC98 HCT 116 C302(0.51); C220(1.01)  LDD0682  [11]
 LDCM0366  AC99 HCT 116 C302(0.65); C220(1.19)  LDD0683  [11]
 LDCM0248  AKOS034007472 HCT 116 C608(1.23)  LDD0565  [11]
 LDCM0356  AKOS034007680 HCT 116 C608(1.38)  LDD0673  [11]
 LDCM0275  AKOS034007705 HCT 116 C608(2.47)  LDD0592  [11]
 LDCM0367  CL1 HEK-293T C994(0.80)  LDD0965  [11]
 LDCM0368  CL10 HEK-293T C994(0.73)  LDD0966  [11]
 LDCM0369  CL100 HEK-293T C994(1.17)  LDD0967  [11]
 LDCM0370  CL101 HCT 116 C608(1.67)  LDD0687  [11]
 LDCM0371  CL102 HCT 116 C608(1.22)  LDD0688  [11]
 LDCM0372  CL103 HCT 116 C608(1.35)  LDD0689  [11]
 LDCM0373  CL104 HCT 116 C608(1.30)  LDD0690  [11]
 LDCM0374  CL105 HCT 116 C220(0.98)  LDD0691  [11]
 LDCM0375  CL106 HCT 116 C220(0.97)  LDD0692  [11]
 LDCM0376  CL107 HCT 116 C220(1.06)  LDD0693  [11]
 LDCM0377  CL108 HCT 116 C220(0.82)  LDD0694  [11]
 LDCM0378  CL109 HCT 116 C220(0.82)  LDD0695  [11]
 LDCM0379  CL11 HEK-293T C994(0.73)  LDD0977  [11]
 LDCM0380  CL110 HCT 116 C220(0.81)  LDD0697  [11]
 LDCM0381  CL111 HCT 116 C220(0.83)  LDD0698  [11]
 LDCM0382  CL112 HCT 116 C302(0.62); C608(1.09); C220(1.36)  LDD0699  [11]
 LDCM0383  CL113 HCT 116 C302(0.41); C608(1.23); C220(1.25)  LDD0700  [11]
 LDCM0384  CL114 HCT 116 C302(0.64); C220(0.98); C608(1.28)  LDD0701  [11]
 LDCM0385  CL115 HCT 116 C302(0.59); C608(1.22); C220(1.41)  LDD0702  [11]
 LDCM0386  CL116 HCT 116 C302(0.64); C608(1.10); C220(1.71)  LDD0703  [11]
 LDCM0387  CL117 HEK-293T C994(1.01)  LDD0985  [11]
 LDCM0388  CL118 HEK-293T C994(0.99)  LDD0986  [11]
 LDCM0389  CL119 HEK-293T C994(1.09)  LDD0987  [11]
 LDCM0390  CL12 HEK-293T C994(0.94)  LDD0988  [11]
 LDCM0391  CL120 HEK-293T C994(1.06)  LDD0989  [11]
 LDCM0392  CL121 HCT 116 C608(1.41)  LDD0709  [11]
 LDCM0393  CL122 HCT 116 C608(1.29)  LDD0710  [11]
 LDCM0394  CL123 HCT 116 C608(1.28)  LDD0711  [11]
 LDCM0395  CL124 HCT 116 C608(1.21)  LDD0712  [11]
 LDCM0396  CL125 HEK-293T C994(1.01); C302(1.79)  LDD0994  [11]
 LDCM0397  CL126 HEK-293T C994(0.88); C302(1.14)  LDD0995  [11]
 LDCM0398  CL127 HEK-293T C994(1.02); C302(1.09)  LDD0996  [11]
 LDCM0399  CL128 HEK-293T C994(1.04); C302(1.05)  LDD0997  [11]
 LDCM0400  CL13 HEK-293T C994(1.00)  LDD0998  [11]
 LDCM0401  CL14 HEK-293T C994(1.19)  LDD0999  [11]
 LDCM0402  CL15 HEK-293T C994(1.22)  LDD1000  [11]
 LDCM0403  CL16 PaTu 8988t C994(1.21); C608(1.08); C978(0.19); C979(0.19)  LDD1282  [11]
 LDCM0404  CL17 PaTu 8988t C994(3.24); C608(1.22); C978(0.15); C979(0.15)  LDD1283  [11]
 LDCM0405  CL18 PaTu 8988t C994(2.41); C608(1.35); C978(0.15); C979(0.15)  LDD1284  [11]
 LDCM0406  CL19 PaTu 8988t C994(4.38); C608(1.02); C978(0.12); C979(0.12)  LDD1285  [11]
 LDCM0407  CL2 HEK-293T C994(0.79)  LDD1005  [11]
 LDCM0408  CL20 PaTu 8988t C994(3.25); C608(0.91); C978(0.10); C979(0.10)  LDD1287  [11]
 LDCM0409  CL21 PaTu 8988t C994(5.92); C608(1.49); C978(0.11); C979(0.11)  LDD1288  [11]
 LDCM0410  CL22 PaTu 8988t C994(5.13); C608(1.34); C978(0.10); C979(0.10)  LDD1289  [11]
 LDCM0411  CL23 PaTu 8988t C994(2.05); C608(1.25); C978(0.12); C979(0.12)  LDD1290  [11]
 LDCM0412  CL24 PaTu 8988t C994(1.21); C608(1.09); C978(0.18); C979(0.18)  LDD1291  [11]
 LDCM0413  CL25 PaTu 8988t C994(1.86); C608(1.28); C978(0.36); C979(0.36)  LDD1292  [11]
 LDCM0414  CL26 PaTu 8988t C994(4.33); C608(1.46); C978(0.10); C979(0.10)  LDD1293  [11]
 LDCM0415  CL27 PaTu 8988t C994(2.26); C608(1.61); C978(0.20); C979(0.20)  LDD1294  [11]
 LDCM0416  CL28 PaTu 8988t C994(3.79); C608(1.47); C978(0.10); C979(0.10)  LDD1295  [11]
 LDCM0417  CL29 PaTu 8988t C994(3.85); C608(1.24); C978(0.14); C979(0.14)  LDD1296  [11]
 LDCM0418  CL3 HEK-293T C994(1.01)  LDD1016  [11]
 LDCM0419  CL30 PaTu 8988t C994(1.96); C608(1.39); C978(0.23); C979(0.23)  LDD1298  [11]
 LDCM0420  CL31 HEK-293T C994(1.12); C302(1.06); C608(0.93)  LDD1018  [11]
 LDCM0421  CL32 HEK-293T C994(1.49); C302(0.99); C608(0.83)  LDD1019  [11]
 LDCM0422  CL33 HEK-293T C994(2.47); C302(1.04); C608(1.13)  LDD1020  [11]
 LDCM0423  CL34 HEK-293T C994(1.26); C302(1.38); C608(1.05)  LDD1021  [11]
 LDCM0424  CL35 HEK-293T C994(1.72); C302(0.82); C608(0.82)  LDD1022  [11]
 LDCM0425  CL36 HEK-293T C994(1.55); C302(1.46); C608(1.02)  LDD1023  [11]
 LDCM0426  CL37 HEK-293T C994(2.00); C302(0.91); C608(0.83)  LDD1024  [11]
 LDCM0428  CL39 HEK-293T C994(4.53); C302(1.74); C608(1.32)  LDD1026  [11]
 LDCM0429  CL4 HEK-293T C994(0.96)  LDD1027  [11]
 LDCM0430  CL40 HEK-293T C994(1.44); C302(1.29); C608(0.88)  LDD1028  [11]
 LDCM0431  CL41 HEK-293T C994(5.18); C302(1.35); C608(0.97)  LDD1029  [11]
 LDCM0432  CL42 HEK-293T C994(3.06); C302(1.80); C608(0.99)  LDD1030  [11]
 LDCM0433  CL43 HEK-293T C994(3.14); C302(1.63); C608(0.98)  LDD1031  [11]
 LDCM0434  CL44 HEK-293T C994(1.51); C302(1.03); C608(0.80)  LDD1032  [11]
 LDCM0435  CL45 HEK-293T C994(3.45); C302(1.71); C608(1.04)  LDD1033  [11]
 LDCM0436  CL46 HCT 116 C220(1.29)  LDD0753  [11]
 LDCM0437  CL47 HCT 116 C220(1.35)  LDD0754  [11]
 LDCM0438  CL48 HCT 116 C220(1.18)  LDD0755  [11]
 LDCM0439  CL49 HCT 116 C220(1.07)  LDD0756  [11]
 LDCM0440  CL5 HEK-293T C994(0.80)  LDD1038  [11]
 LDCM0441  CL50 HCT 116 C220(0.92)  LDD0758  [11]
 LDCM0442  CL51 HCT 116 C220(1.00)  LDD0759  [11]
 LDCM0443  CL52 HCT 116 C220(1.09)  LDD0760  [11]
 LDCM0444  CL53 HCT 116 C220(0.76)  LDD0761  [11]
 LDCM0445  CL54 HCT 116 C220(1.10)  LDD0762  [11]
 LDCM0446  CL55 HCT 116 C220(1.00)  LDD0763  [11]
 LDCM0447  CL56 HCT 116 C220(1.02)  LDD0764  [11]
 LDCM0448  CL57 HCT 116 C220(1.07)  LDD0765  [11]
 LDCM0449  CL58 HCT 116 C220(1.45)  LDD0766  [11]
 LDCM0450  CL59 HCT 116 C220(1.19)  LDD0767  [11]
 LDCM0451  CL6 HEK-293T C994(1.03)  LDD1049  [11]
 LDCM0452  CL60 HCT 116 C220(1.02)  LDD0769  [11]
 LDCM0453  CL61 HEK-293T C994(0.96); C302(0.46)  LDD1051  [11]
 LDCM0454  CL62 HEK-293T C994(1.22); C302(0.50)  LDD1052  [11]
 LDCM0455  CL63 HEK-293T C994(1.06); C302(0.66)  LDD1053  [11]
 LDCM0456  CL64 HEK-293T C994(2.43); C302(0.88)  LDD1054  [11]
 LDCM0457  CL65 HEK-293T C994(1.14); C302(0.53)  LDD1055  [11]
 LDCM0458  CL66 HEK-293T C994(1.30); C302(1.02)  LDD1056  [11]
 LDCM0459  CL67 HEK-293T C994(1.18); C302(0.94)  LDD1057  [11]
 LDCM0460  CL68 HEK-293T C994(1.23); C302(0.62)  LDD1058  [11]
 LDCM0461  CL69 HEK-293T C994(1.18); C302(0.85)  LDD1059  [11]
 LDCM0462  CL7 HEK-293T C994(0.67)  LDD1060  [11]
 LDCM0463  CL70 HEK-293T C994(1.12); C302(0.83)  LDD1061  [11]
 LDCM0464  CL71 HEK-293T C994(1.45); C302(1.00)  LDD1062  [11]
 LDCM0465  CL72 HEK-293T C994(1.03); C302(0.76)  LDD1063  [11]
 LDCM0466  CL73 HEK-293T C994(1.31); C302(0.62)  LDD1064  [11]
 LDCM0467  CL74 HEK-293T C994(2.10); C302(0.59)  LDD1065  [11]
 LDCM0469  CL76 HCT 116 C220(1.27); C608(1.14)  LDD0786  [11]
 LDCM0470  CL77 HCT 116 C220(0.77); C608(0.99)  LDD0787  [11]
 LDCM0471  CL78 HCT 116 C220(0.89); C608(0.94)  LDD0788  [11]
 LDCM0472  CL79 HCT 116 C220(0.90); C608(0.88)  LDD0789  [11]
 LDCM0473  CL8 HEK-293T C994(1.88)  LDD1071  [11]
 LDCM0474  CL80 HCT 116 C220(0.60); C608(0.92)  LDD0791  [11]
 LDCM0475  CL81 HCT 116 C220(0.94); C608(0.91)  LDD0792  [11]
 LDCM0476  CL82 HCT 116 C220(1.27); C608(1.15)  LDD0793  [11]
 LDCM0477  CL83 HCT 116 C220(0.73); C608(1.05)  LDD0794  [11]
 LDCM0478  CL84 HCT 116 C220(0.90); C608(1.03)  LDD0795  [11]
 LDCM0479  CL85 HCT 116 C220(0.96); C608(0.96)  LDD0796  [11]
 LDCM0480  CL86 HCT 116 C220(1.01); C608(0.84)  LDD0797  [11]
 LDCM0481  CL87 HCT 116 C220(0.71); C608(0.93)  LDD0798  [11]
 LDCM0482  CL88 HCT 116 C220(0.68); C608(0.93)  LDD0799  [11]
 LDCM0483  CL89 HCT 116 C220(0.69); C608(0.91)  LDD0800  [11]
 LDCM0484  CL9 HEK-293T C994(1.25)  LDD1082  [11]
 LDCM0485  CL90 HCT 116 C220(0.78); C608(1.30)  LDD0802  [11]
 LDCM0486  CL91 HEK-293T C994(0.79)  LDD1084  [11]
 LDCM0487  CL92 HEK-293T C994(1.10)  LDD1085  [11]
 LDCM0488  CL93 HEK-293T C994(1.27)  LDD1086  [11]
 LDCM0489  CL94 HEK-293T C994(1.08)  LDD1087  [11]
 LDCM0490  CL95 HEK-293T C994(2.91)  LDD1088  [11]
 LDCM0491  CL96 HEK-293T C994(1.18)  LDD1089  [11]
 LDCM0492  CL97 HEK-293T C994(0.96)  LDD1090  [11]
 LDCM0493  CL98 HEK-293T C994(1.04)  LDD1091  [11]
 LDCM0494  CL99 HEK-293T C994(1.24)  LDD1092  [11]
 LDCM0495  E2913 HEK-293T C608(1.01); C994(2.38); C552(0.92); C302(1.03)  LDD1698  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C585(9.87); C220(1.79)  LDD1702  [13]
 LDCM0468  Fragment33 HEK-293T C994(1.60); C302(0.72)  LDD1066  [11]
 LDCM0427  Fragment51 HEK-293T C994(1.23); C302(1.29); C608(0.91)  LDD1025  [11]
 LDCM0617  Fragment63-S Jurkat _(4.06)  LDD1490  [2]
 LDCM0022  KB02 HEK-293T C608(0.94); C994(0.93); C585(1.04)  LDD1492  [12]
 LDCM0023  KB03 Jurkat C608(31.49); C302(10.46)  LDD0209  [4]
 LDCM0024  KB05 COLO792 C468(2.21); C302(2.21)  LDD3310  [14]
 LDCM0628  OTUB2-COV-1 HEK-293T C994(1.50)  LDD2207  [15]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
13 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
14 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
15 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.