Details of the Target

General Information of Target

Target ID LDTP13204
Target Name Gamma-adducin (ADD3)
Gene Name ADD3
Gene ID 120
Synonyms
ADDL; Gamma-adducin; Adducin-like protein 70
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAAQAVGWPICR
DAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPN
VVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGL
DYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMA
PEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDK
EMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQR
AKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSAS
TIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTAD
GVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFA
QLSVS
Target Bioclass
Enzyme
Family
Aldolase class II family, Adducin subfamily
Subcellular location
Cytoplasm, cytoskeleton
Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. Binds to calmodulin (Probable). Involved in myogenic reactivity of the renal afferent arteriole (Af-art), renal interlobular arteries and middle cerebral artery (MCA) to increased perfusion pressure. Involved in regulation of potassium channels in the vascular smooth muscle cells (VSMCs) of the Af-art and MCA ex vivo. Involved in regulation of glomerular capillary pressure, glomerular filtration rate (GFR) and glomerular nephrin expression in response to hypertension. Involved in renal blood flow (RBF) autoregulation. Plays a role in podocyte structure and function. Regulates globular monomer actin (G-actin) and filamentous polymer actin (F-actin) ratios in the primary podocytes affecting actin cytoskeleton organization. Regulates expression of synaptopodin, RhoA, Rac1 and CDC42 in the renal cortex and the primary podocytes. Regulates expression of nephrin in the glomeruli and in the primary podocytes, expression of nephrin and podocinin in the renal cortex, and expression of focal adhesion proteins integrin alpha-3 and integrin beta-1 in the glomeruli. Involved in cell migration and cell adhesion of podocytes, and in podocyte foot process effacement. Regulates expression of profibrotics markers MMP2, MMP9, TGF beta-1, tubular tight junction protein E-cadherin, and mesenchymal markers vimentin and alpha-SMA. Promotes the growth of neurites.
Uniprot ID
Q9UEY8
Ensemble ID
ENST00000277900.12
HGNC ID
HGNC:245

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL62 SNV: p.P85L DBIA    Probe Info 
DU145 SNV: p.S474L; p.T653I DBIA    Probe Info 
MCC13 SNV: p.K397Q DBIA    Probe Info 
NALM6 SNV: p.R138H DBIA    Probe Info 
OPM2 SNV: p.E623V DBIA    Probe Info 
RKO SNV: p.L111R DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 18 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K465(3.22); K470(1.10)  LDD0277  [1]
Probe 1
 Probe Info  		Y35(13.43)  LDD3495  [2]
BTD
 Probe Info  		C286(0.84)  LDD2093  [3]
AHL-Pu-1
 Probe Info  		C73(2.31)  LDD0168  [4]
DBIA
 Probe Info  		C73(1.07)  LDD0080  [5]
1d-yne
 Probe Info  		N.A.  LDD0358  [6]
4-Iodoacetamidophenylacetylene
 Probe Info  		C73(0.00); C286(0.00); C245(0.00)  LDD0038  [7]
IA-alkyne
 Probe Info  		C73(0.00); C286(0.00)  LDD0036  [7]
Lodoacetamide azide
 Probe Info  		C245(0.00); C286(0.00); C73(0.00)  LDD0037  [7]
NAIA_5
 Probe Info  		N.A.  LDD2225  [8]
IPM
 Probe Info  		N.A.  LDD2156  [9]
TFBX
 Probe Info  		N.A.  LDD0148  [10]
VSF
 Probe Info  		N.A.  LDD0007  [11]
Phosphinate-6
 Probe Info  		C139(0.00); C286(0.00)  LDD0018  [12]
1c-yne
 Probe Info  		N.A.  LDD0228  [6]
Acrolein
 Probe Info  		N.A.  LDD0217  [13]
Methacrolein
 Probe Info  		N.A.  LDD0218  [13]
AOyne
 Probe Info  		13.70  LDD0443  [14]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C040
 Probe Info  		10.41  LDD1740  [15]
C145
 Probe Info  		9.71  LDD1827  [15]
C382
 Probe Info  		9.78  LDD2041  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C286(0.89)  LDD2152  [3]
 LDCM0025  4SU-RNA HEK-293T C73(2.31)  LDD0168  [4]
 LDCM0026  4SU-RNA+native RNA HEK-293T C73(2.90)  LDD0169  [4]
 LDCM0214  AC1 HEK-293T C286(0.91); C73(0.86); C245(0.99)  LDD1507  [16]
 LDCM0215  AC10 HCT 116 C286(0.98)  LDD0532  [5]
 LDCM0226  AC11 HCT 116 C286(0.96)  LDD0543  [5]
 LDCM0230  AC113 PaTu 8988t C286(1.11)  LDD1109  [5]
 LDCM0231  AC114 PaTu 8988t C286(1.08)  LDD1110  [5]
 LDCM0232  AC115 PaTu 8988t C286(0.97)  LDD1111  [5]
 LDCM0233  AC116 PaTu 8988t C286(1.18)  LDD1112  [5]
 LDCM0234  AC117 PaTu 8988t C286(1.08)  LDD1113  [5]
 LDCM0235  AC118 PaTu 8988t C286(1.00)  LDD1114  [5]
 LDCM0236  AC119 PaTu 8988t C286(1.04)  LDD1115  [5]
 LDCM0237  AC12 HCT 116 C286(0.98)  LDD0554  [5]
 LDCM0238  AC120 PaTu 8988t C286(1.04)  LDD1117  [5]
 LDCM0239  AC121 PaTu 8988t C286(1.11)  LDD1118  [5]
 LDCM0240  AC122 PaTu 8988t C286(1.09)  LDD1119  [5]
 LDCM0241  AC123 PaTu 8988t C286(0.94)  LDD1120  [5]
 LDCM0242  AC124 PaTu 8988t C286(1.08)  LDD1121  [5]
 LDCM0243  AC125 PaTu 8988t C286(1.02)  LDD1122  [5]
 LDCM0244  AC126 PaTu 8988t C286(1.13)  LDD1123  [5]
 LDCM0245  AC127 PaTu 8988t C286(1.00)  LDD1124  [5]
 LDCM0246  AC128 PaTu 8988t C286(1.03)  LDD1125  [5]
 LDCM0247  AC129 PaTu 8988t C286(1.01)  LDD1126  [5]
 LDCM0249  AC130 PaTu 8988t C286(0.99)  LDD1128  [5]
 LDCM0250  AC131 PaTu 8988t C286(0.92)  LDD1129  [5]
 LDCM0251  AC132 PaTu 8988t C286(1.04)  LDD1130  [5]
 LDCM0252  AC133 PaTu 8988t C286(1.23)  LDD1131  [5]
 LDCM0253  AC134 PaTu 8988t C286(1.11)  LDD1132  [5]
 LDCM0254  AC135 PaTu 8988t C286(1.12)  LDD1133  [5]
 LDCM0255  AC136 PaTu 8988t C286(1.11)  LDD1134  [5]
 LDCM0256  AC137 PaTu 8988t C286(1.20)  LDD1135  [5]
 LDCM0257  AC138 PaTu 8988t C286(0.97)  LDD1136  [5]
 LDCM0258  AC139 PaTu 8988t C286(0.98)  LDD1137  [5]
 LDCM0259  AC14 HCT 116 C286(1.03)  LDD0576  [5]
 LDCM0260  AC140 PaTu 8988t C286(0.95)  LDD1139  [5]
 LDCM0261  AC141 PaTu 8988t C286(0.91)  LDD1140  [5]
 LDCM0262  AC142 PaTu 8988t C286(1.07)  LDD1141  [5]
 LDCM0263  AC143 HCT 116 C286(0.94)  LDD0580  [5]
 LDCM0264  AC144 HCT 116 C286(0.85)  LDD0581  [5]
 LDCM0265  AC145 HCT 116 C286(0.87)  LDD0582  [5]
 LDCM0266  AC146 HCT 116 C286(0.89)  LDD0583  [5]
 LDCM0267  AC147 HCT 116 C286(0.93)  LDD0584  [5]
 LDCM0268  AC148 HCT 116 C286(1.12)  LDD0585  [5]
 LDCM0269  AC149 HCT 116 C286(1.04)  LDD0586  [5]
 LDCM0270  AC15 HCT 116 C286(1.02)  LDD0587  [5]
 LDCM0271  AC150 HCT 116 C286(0.90)  LDD0588  [5]
 LDCM0272  AC151 HCT 116 C286(0.80)  LDD0589  [5]
 LDCM0273  AC152 HCT 116 C286(0.93)  LDD0590  [5]
 LDCM0274  AC153 HCT 116 C286(1.14)  LDD0591  [5]
 LDCM0621  AC154 HCT 116 C286(0.92)  LDD2158  [5]
 LDCM0622  AC155 HCT 116 C286(0.85)  LDD2159  [5]
 LDCM0623  AC156 HCT 116 C286(0.90)  LDD2160  [5]
 LDCM0624  AC157 HCT 116 C286(0.86)  LDD2161  [5]
 LDCM0276  AC17 HEK-293T C286(0.94); C73(0.98); C245(1.16)  LDD1515  [16]
 LDCM0277  AC18 HEK-293T C286(0.99); C73(1.00); C245(0.89)  LDD1516  [16]
 LDCM0278  AC19 HEK-293T C286(0.96); C73(0.94); C245(0.86)  LDD1517  [16]
 LDCM0279  AC2 HEK-293T C286(0.98); C73(1.03); C245(0.90)  LDD1518  [16]
 LDCM0280  AC20 HEK-293T C286(1.00); C73(1.06); C245(1.11)  LDD1519  [16]
 LDCM0281  AC21 HEK-293T C286(1.00); C73(1.03); C245(0.98)  LDD1520  [16]
 LDCM0282  AC22 HEK-293T C286(0.94); C73(1.01)  LDD1521  [16]
 LDCM0283  AC23 HEK-293T C286(0.98); C73(1.03); C245(1.08)  LDD1522  [16]
 LDCM0284  AC24 HEK-293T C286(1.03); C73(0.98); C245(1.07)  LDD1523  [16]
 LDCM0285  AC25 HCT 116 C286(0.98)  LDD0602  [5]
 LDCM0286  AC26 HCT 116 C286(0.89)  LDD0603  [5]
 LDCM0287  AC27 HCT 116 C286(0.92)  LDD0604  [5]
 LDCM0288  AC28 HCT 116 C286(0.93)  LDD0605  [5]
 LDCM0289  AC29 HCT 116 C286(0.91)  LDD0606  [5]
 LDCM0290  AC3 HEK-293T C286(0.88); C73(1.02); C245(1.21)  LDD1529  [16]
 LDCM0291  AC30 HCT 116 C286(0.91)  LDD0608  [5]
 LDCM0292  AC31 HCT 116 C286(0.93)  LDD0609  [5]
 LDCM0293  AC32 HCT 116 C286(0.95)  LDD0610  [5]
 LDCM0294  AC33 HCT 116 C286(0.89)  LDD0611  [5]
 LDCM0295  AC34 HCT 116 C286(0.92)  LDD0612  [5]
 LDCM0296  AC35 HCT 116 C286(1.01)  LDD0613  [5]
 LDCM0297  AC36 HCT 116 C286(1.00)  LDD0614  [5]
 LDCM0298  AC37 HCT 116 C286(0.96)  LDD0615  [5]
 LDCM0299  AC38 HCT 116 C286(0.89)  LDD0616  [5]
 LDCM0300  AC39 HCT 116 C286(1.08)  LDD0617  [5]
 LDCM0301  AC4 HEK-293T C286(1.01); C73(1.00); C245(1.01)  LDD1540  [16]
 LDCM0302  AC40 HCT 116 C286(1.05)  LDD0619  [5]
 LDCM0303  AC41 HCT 116 C286(1.01)  LDD0620  [5]
 LDCM0304  AC42 HCT 116 C286(0.98)  LDD0621  [5]
 LDCM0305  AC43 HCT 116 C286(0.95)  LDD0622  [5]
 LDCM0306  AC44 HCT 116 C286(1.03)  LDD0623  [5]
 LDCM0307  AC45 HCT 116 C286(1.08)  LDD0624  [5]
 LDCM0308  AC46 HCT 116 C286(0.97)  LDD0625  [5]
 LDCM0309  AC47 HCT 116 C286(1.14)  LDD0626  [5]
 LDCM0310  AC48 HCT 116 C286(1.14)  LDD0627  [5]
 LDCM0311  AC49 HCT 116 C286(1.24)  LDD0628  [5]
 LDCM0312  AC5 HEK-293T C286(1.06); C73(1.00); C245(1.06)  LDD1551  [16]
 LDCM0313  AC50 HCT 116 C286(1.09)  LDD0630  [5]
 LDCM0314  AC51 HCT 116 C286(1.19)  LDD0631  [5]
 LDCM0315  AC52 HCT 116 C286(1.09)  LDD0632  [5]
 LDCM0316  AC53 HCT 116 C286(1.01)  LDD0633  [5]
 LDCM0317  AC54 HCT 116 C286(1.07)  LDD0634  [5]
 LDCM0318  AC55 HCT 116 C286(1.11)  LDD0635  [5]
 LDCM0319  AC56 HCT 116 C286(1.34)  LDD0636  [5]
 LDCM0320  AC57 HCT 116 C286(1.07)  LDD0637  [5]
 LDCM0321  AC58 HCT 116 C286(1.03)  LDD0638  [5]
 LDCM0322  AC59 HCT 116 C286(1.14)  LDD0639  [5]
 LDCM0323  AC6 HCT 116 C286(0.97)  LDD0640  [5]
 LDCM0324  AC60 HCT 116 C286(1.02)  LDD0641  [5]
 LDCM0325  AC61 HCT 116 C286(0.96)  LDD0642  [5]
 LDCM0326  AC62 HCT 116 C286(0.97)  LDD0643  [5]
 LDCM0327  AC63 HCT 116 C286(1.00)  LDD0644  [5]
 LDCM0328  AC64 HCT 116 C286(1.32)  LDD0645  [5]
 LDCM0329  AC65 HCT 116 C286(1.35)  LDD0646  [5]
 LDCM0330  AC66 HCT 116 C286(1.38)  LDD0647  [5]
 LDCM0331  AC67 HCT 116 C286(1.02)  LDD0648  [5]
 LDCM0332  AC68 PaTu 8988t C73(0.98)  LDD1211  [5]
 LDCM0333  AC69 PaTu 8988t C73(1.12)  LDD1212  [5]
 LDCM0334  AC7 HCT 116 C286(0.98)  LDD0651  [5]
 LDCM0335  AC70 PaTu 8988t C73(1.12)  LDD1214  [5]
 LDCM0336  AC71 PaTu 8988t C73(1.09)  LDD1215  [5]
 LDCM0337  AC72 PaTu 8988t C73(1.05)  LDD1216  [5]
 LDCM0338  AC73 PaTu 8988t C73(0.95)  LDD1217  [5]
 LDCM0339  AC74 PaTu 8988t C73(1.15)  LDD1218  [5]
 LDCM0340  AC75 PaTu 8988t C73(1.19)  LDD1219  [5]
 LDCM0341  AC76 PaTu 8988t C73(1.04)  LDD1220  [5]
 LDCM0342  AC77 PaTu 8988t C73(0.95)  LDD1221  [5]
 LDCM0343  AC78 PaTu 8988t C73(1.17)  LDD1222  [5]
 LDCM0344  AC79 PaTu 8988t C73(1.21)  LDD1223  [5]
 LDCM0345  AC8 HCT 116 C286(0.96)  LDD0662  [5]
 LDCM0346  AC80 PaTu 8988t C73(1.14)  LDD1225  [5]
 LDCM0347  AC81 PaTu 8988t C73(1.22)  LDD1226  [5]
 LDCM0348  AC82 PaTu 8988t C73(1.35)  LDD1227  [5]
 LDCM0349  AC83 HCT 116 C286(1.07)  LDD0666  [5]
 LDCM0350  AC84 HCT 116 C286(1.07)  LDD0667  [5]
 LDCM0351  AC85 HCT 116 C286(1.06)  LDD0668  [5]
 LDCM0352  AC86 HCT 116 C286(1.00)  LDD0669  [5]
 LDCM0353  AC87 HCT 116 C286(1.00)  LDD0670  [5]
 LDCM0354  AC88 HCT 116 C286(0.92)  LDD0671  [5]
 LDCM0355  AC89 HCT 116 C286(1.05)  LDD0672  [5]
 LDCM0357  AC90 HCT 116 C286(0.90)  LDD0674  [5]
 LDCM0358  AC91 HCT 116 C286(1.17)  LDD0675  [5]
 LDCM0359  AC92 HCT 116 C286(1.09)  LDD0676  [5]
 LDCM0360  AC93 HCT 116 C286(0.90)  LDD0677  [5]
 LDCM0361  AC94 HCT 116 C286(1.20)  LDD0678  [5]
 LDCM0362  AC95 HCT 116 C286(1.02)  LDD0679  [5]
 LDCM0363  AC96 HCT 116 C286(1.05)  LDD0680  [5]
 LDCM0364  AC97 HCT 116 C286(0.96)  LDD0681  [5]
 LDCM0248  AKOS034007472 HCT 116 C286(1.02)  LDD0565  [5]
 LDCM0356  AKOS034007680 HCT 116 C286(0.96)  LDD0673  [5]
 LDCM0275  AKOS034007705 HCT 116 C286(1.17)  LDD0592  [5]
 LDCM0108  Chloroacetamide HeLa C286(0.00); C73(0.00)  LDD0222  [13]
 LDCM0367  CL1 PaTu 8988t C73(0.94); C286(0.85)  LDD1246  [5]
 LDCM0368  CL10 PaTu 8988t C73(1.35); C286(0.81)  LDD1247  [5]
 LDCM0369  CL100 HEK-293T C286(0.97); C73(1.00); C245(1.00)  LDD1573  [16]
 LDCM0370  CL101 HCT 116 C286(1.01)  LDD0687  [5]
 LDCM0371  CL102 HCT 116 C286(0.98)  LDD0688  [5]
 LDCM0372  CL103 HCT 116 C286(0.92)  LDD0689  [5]
 LDCM0373  CL104 HCT 116 C286(0.94)  LDD0690  [5]
 LDCM0374  CL105 HEK-293T C286(0.96); C73(0.92); C245(1.09)  LDD1578  [16]
 LDCM0375  CL106 HEK-293T C286(0.99); C73(1.00); C245(1.07)  LDD1579  [16]
 LDCM0376  CL107 HEK-293T C286(1.01); C73(1.01); C245(1.09)  LDD1580  [16]
 LDCM0377  CL108 HEK-293T C286(0.98); C73(0.99); C245(0.97)  LDD1581  [16]
 LDCM0378  CL109 HEK-293T C286(1.03); C73(0.88); C245(1.07)  LDD1582  [16]
 LDCM0379  CL11 PaTu 8988t C73(0.91); C286(0.94)  LDD1258  [5]
 LDCM0380  CL110 HEK-293T C286(1.05); C73(0.98); C245(0.99)  LDD1584  [16]
 LDCM0381  CL111 HEK-293T C286(1.01); C73(0.99); C245(1.10)  LDD1585  [16]
 LDCM0382  CL112 HCT 116 C286(0.92)  LDD0699  [5]
 LDCM0383  CL113 HCT 116 C286(0.92)  LDD0700  [5]
 LDCM0384  CL114 HCT 116 C286(0.89)  LDD0701  [5]
 LDCM0385  CL115 HCT 116 C286(0.93)  LDD0702  [5]
 LDCM0386  CL116 HCT 116 C286(0.91)  LDD0703  [5]
 LDCM0387  CL117 HCT 116 C286(1.19)  LDD0704  [5]
 LDCM0388  CL118 HCT 116 C286(1.03)  LDD0705  [5]
 LDCM0389  CL119 HCT 116 C286(1.07)  LDD0706  [5]
 LDCM0390  CL12 PaTu 8988t C73(1.01); C286(0.88)  LDD1269  [5]
 LDCM0391  CL120 HCT 116 C286(1.05)  LDD0708  [5]
 LDCM0392  CL121 HCT 116 C286(1.14)  LDD0709  [5]
 LDCM0393  CL122 HCT 116 C286(1.02)  LDD0710  [5]
 LDCM0394  CL123 HCT 116 C286(1.00)  LDD0711  [5]
 LDCM0395  CL124 HCT 116 C286(1.02)  LDD0712  [5]
 LDCM0396  CL125 HCT 116 C286(1.05)  LDD0713  [5]
 LDCM0397  CL126 HCT 116 C286(0.94)  LDD0714  [5]
 LDCM0398  CL127 HCT 116 C286(0.85)  LDD0715  [5]
 LDCM0399  CL128 HCT 116 C286(0.88)  LDD0716  [5]
 LDCM0400  CL13 PaTu 8988t C73(1.63); C286(0.98)  LDD1279  [5]
 LDCM0401  CL14 PaTu 8988t C73(1.66); C286(0.95)  LDD1280  [5]
 LDCM0402  CL15 PaTu 8988t C73(1.15); C286(0.92)  LDD1281  [5]
 LDCM0403  CL16 HCT 116 C286(1.01)  LDD0720  [5]
 LDCM0404  CL17 HCT 116 C286(0.64)  LDD0721  [5]
 LDCM0405  CL18 HCT 116 C286(0.94)  LDD0722  [5]
 LDCM0406  CL19 HCT 116 C286(0.86)  LDD0723  [5]
 LDCM0407  CL2 PaTu 8988t C73(0.98); C286(0.87)  LDD1286  [5]
 LDCM0408  CL20 HCT 116 C286(0.82)  LDD0725  [5]
 LDCM0409  CL21 HCT 116 C286(0.76)  LDD0726  [5]
 LDCM0410  CL22 HCT 116 C286(1.08)  LDD0727  [5]
 LDCM0411  CL23 HCT 116 C286(0.91)  LDD0728  [5]
 LDCM0412  CL24 HCT 116 C286(0.86)  LDD0729  [5]
 LDCM0413  CL25 HCT 116 C286(1.01)  LDD0730  [5]
 LDCM0414  CL26 HCT 116 C286(0.85)  LDD0731  [5]
 LDCM0415  CL27 HCT 116 C286(0.95)  LDD0732  [5]
 LDCM0416  CL28 HCT 116 C286(0.86)  LDD0733  [5]
 LDCM0417  CL29 HCT 116 C286(0.91)  LDD0734  [5]
 LDCM0418  CL3 PaTu 8988t C73(1.29); C286(0.91)  LDD1297  [5]
 LDCM0419  CL30 HCT 116 C286(0.96)  LDD0736  [5]
 LDCM0420  CL31 PaTu 8988t C73(1.11); C286(1.00)  LDD1299  [5]
 LDCM0421  CL32 PaTu 8988t C73(1.90); C286(1.18)  LDD1300  [5]
 LDCM0422  CL33 PaTu 8988t C73(1.59); C286(1.02)  LDD1301  [5]
 LDCM0423  CL34 PaTu 8988t C73(1.98); C286(1.04)  LDD1302  [5]
 LDCM0424  CL35 PaTu 8988t C286(1.05); C73(1.66)  LDD1303  [5]
 LDCM0425  CL36 PaTu 8988t C73(1.27); C286(0.99)  LDD1304  [5]
 LDCM0426  CL37 PaTu 8988t C73(2.04); C286(1.27)  LDD1305  [5]
 LDCM0428  CL39 PaTu 8988t C73(1.54); C286(0.98)  LDD1307  [5]
 LDCM0429  CL4 PaTu 8988t C73(1.14); C286(0.93)  LDD1308  [5]
 LDCM0430  CL40 PaTu 8988t C73(1.23); C286(1.04)  LDD1309  [5]
 LDCM0431  CL41 PaTu 8988t C73(1.73); C286(1.14)  LDD1310  [5]
 LDCM0432  CL42 PaTu 8988t C73(1.74); C286(1.30)  LDD1311  [5]
 LDCM0433  CL43 PaTu 8988t C73(2.27); C286(1.38)  LDD1312  [5]
 LDCM0434  CL44 PaTu 8988t C73(1.78); C286(1.11)  LDD1313  [5]
 LDCM0435  CL45 PaTu 8988t C73(1.10); C286(1.03)  LDD1314  [5]
 LDCM0436  CL46 PaTu 8988t C73(1.06); C286(1.01)  LDD1315  [5]
 LDCM0437  CL47 PaTu 8988t C73(0.93); C286(1.03)  LDD1316  [5]
 LDCM0438  CL48 PaTu 8988t C73(0.95); C286(1.05)  LDD1317  [5]
 LDCM0439  CL49 PaTu 8988t C73(1.07); C286(1.00)  LDD1318  [5]
 LDCM0440  CL5 PaTu 8988t C73(1.63); C286(0.89)  LDD1319  [5]
 LDCM0441  CL50 PaTu 8988t C73(1.08); C286(0.91)  LDD1320  [5]
 LDCM0442  CL51 PaTu 8988t C73(0.99); C286(0.98)  LDD1321  [5]
 LDCM0443  CL52 PaTu 8988t C73(1.03); C286(1.05)  LDD1322  [5]
 LDCM0444  CL53 PaTu 8988t C73(1.22); C286(1.01)  LDD1323  [5]
 LDCM0445  CL54 PaTu 8988t C73(0.85); C286(1.09)  LDD1324  [5]
 LDCM0446  CL55 PaTu 8988t C73(1.00); C286(0.99)  LDD1325  [5]
 LDCM0447  CL56 PaTu 8988t C73(0.76); C286(1.07)  LDD1326  [5]
 LDCM0448  CL57 PaTu 8988t C73(1.02); C286(0.96)  LDD1327  [5]
 LDCM0449  CL58 PaTu 8988t C73(0.97); C286(0.95)  LDD1328  [5]
 LDCM0450  CL59 PaTu 8988t C73(0.99); C286(0.94)  LDD1329  [5]
 LDCM0451  CL6 PaTu 8988t C73(1.23); C286(0.89)  LDD1330  [5]
 LDCM0452  CL60 PaTu 8988t C73(0.94); C286(1.03)  LDD1331  [5]
 LDCM0453  CL61 HCT 116 C286(0.90)  LDD0770  [5]
 LDCM0454  CL62 HCT 116 C286(0.88)  LDD0771  [5]
 LDCM0455  CL63 HCT 116 C286(1.01)  LDD0772  [5]
 LDCM0456  CL64 HCT 116 C286(1.01)  LDD0773  [5]
 LDCM0457  CL65 HCT 116 C286(0.94)  LDD0774  [5]
 LDCM0458  CL66 HCT 116 C286(1.03)  LDD0775  [5]
 LDCM0459  CL67 HCT 116 C286(1.00)  LDD0776  [5]
 LDCM0460  CL68 HCT 116 C286(0.93)  LDD0777  [5]
 LDCM0461  CL69 HCT 116 C286(1.02)  LDD0778  [5]
 LDCM0462  CL7 PaTu 8988t C73(1.31); C286(0.99)  LDD1341  [5]
 LDCM0463  CL70 HCT 116 C286(0.91)  LDD0780  [5]
 LDCM0464  CL71 HCT 116 C286(1.06)  LDD0781  [5]
 LDCM0465  CL72 HCT 116 C286(1.02)  LDD0782  [5]
 LDCM0466  CL73 HCT 116 C286(0.81)  LDD0783  [5]
 LDCM0467  CL74 HCT 116 C286(0.86)  LDD0784  [5]
 LDCM0469  CL76 PaTu 8988t C286(1.17)  LDD1348  [5]
 LDCM0470  CL77 PaTu 8988t C286(0.71)  LDD1349  [5]
 LDCM0471  CL78 PaTu 8988t C286(0.83)  LDD1350  [5]
 LDCM0472  CL79 PaTu 8988t C286(0.92)  LDD1351  [5]
 LDCM0473  CL8 PaTu 8988t C73(1.34); C286(0.82)  LDD1352  [5]
 LDCM0474  CL80 PaTu 8988t C286(1.02)  LDD1353  [5]
 LDCM0475  CL81 PaTu 8988t C286(1.04)  LDD1354  [5]
 LDCM0476  CL82 PaTu 8988t C286(0.82)  LDD1355  [5]
 LDCM0477  CL83 PaTu 8988t C286(0.98)  LDD1356  [5]
 LDCM0478  CL84 PaTu 8988t C286(1.13)  LDD1357  [5]
 LDCM0479  CL85 PaTu 8988t C286(0.90)  LDD1358  [5]
 LDCM0480  CL86 PaTu 8988t C286(0.87)  LDD1359  [5]
 LDCM0481  CL87 PaTu 8988t C286(0.98)  LDD1360  [5]
 LDCM0482  CL88 PaTu 8988t C286(0.92)  LDD1361  [5]
 LDCM0483  CL89 PaTu 8988t C286(0.93)  LDD1362  [5]
 LDCM0484  CL9 PaTu 8988t C73(1.24); C286(0.85)  LDD1363  [5]
 LDCM0485  CL90 PaTu 8988t C286(1.02)  LDD1364  [5]
 LDCM0486  CL91 HEK-293T C286(0.91); C73(1.02); C245(0.97)  LDD1689  [16]
 LDCM0487  CL92 HEK-293T C286(0.98); C73(0.97); C245(0.96)  LDD1690  [16]
 LDCM0488  CL93 HEK-293T C286(0.99); C73(1.08); C245(1.26)  LDD1691  [16]
 LDCM0489  CL94 HEK-293T C286(0.99); C73(1.06)  LDD1692  [16]
 LDCM0490  CL95 HEK-293T C286(1.10); C73(0.94); C245(1.35)  LDD1693  [16]
 LDCM0491  CL96 HEK-293T C286(1.07); C73(0.99); C245(0.98)  LDD1694  [16]
 LDCM0492  CL97 HEK-293T C286(0.98); C73(0.90); C245(1.04)  LDD1695  [16]
 LDCM0493  CL98 HEK-293T C286(1.01); C73(0.99); C245(0.93)  LDD1696  [16]
 LDCM0494  CL99 HEK-293T C286(0.97); C73(0.98); C245(0.95)  LDD1697  [16]
 LDCM0495  E2913 HEK-293T C286(0.92); C73(1.00); C245(1.16)  LDD1698  [16]
 LDCM0468  Fragment33 HCT 116 C286(0.87)  LDD0785  [5]
 LDCM0427  Fragment51 PaTu 8988t C73(2.27); C286(0.94)  LDD1306  [5]
 LDCM0022  KB02 HCT 116 C73(1.07)  LDD0080  [5]
 LDCM0023  KB03 HCT 116 C73(1.69)  LDD0081  [5]
 LDCM0024  KB05 HCT 116 C73(1.65)  LDD0082  [5]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C286(0.84)  LDD2093  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C286(0.32)  LDD2109  [3]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C286(1.03)  LDD2111  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C286(0.79)  LDD2127  [3]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C286(0.94)  LDD2136  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C286(0.64)  LDD2140  [3]

References

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Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
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Mass spectrometry data entry: PXD028853
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
13 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
14 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
15 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
16 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402