Details of the Target

General Information of Target

Target ID LDTP12252
Target Name Chromodomain-helicase-DNA-binding protein 8 (CHD8)
Gene Name CHD8
Gene ID 57680
Synonyms
HELSNF1; KIAA1564; Chromodomain-helicase-DNA-binding protein 8; CHD-8; EC 3.6.4.12; ATP-dependent helicase CHD8; Helicase with SNF2 domain 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRV
NREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKV
SVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYH
PLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPL
TDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQ
YFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATAR
SAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVA
TPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQ
PCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQ
VKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKK
PSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTR
FKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCA
DKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADE
LQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRD
PQALAKAVQIHHDTQHTMYFA
Target Bioclass
Enzyme
Family
SNF2/RAD54 helicase family, CHD8 subfamily
Subcellular location
Nucleus
Function
DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. Regulates alternative splicing of a core group of genes involved in neuronal differentiation, cell cycle and DNA repair. Enables H3K36me3-coupled transcription elongation and co-transcriptional RNA processing likely via interaction with HNRNPL. {|HAMAP-Rule:MF_03071}.
Uniprot ID
Q9HCK8
Ensemble ID
ENST00000430710.8
HGNC ID
HGNC:20153

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL51 Deletion: p.R2352DfsTer3 DBIA    Probe Info 
CCK81 Deletion: p.K443RfsTer23 .
EFO27 Deletion: p.K2025_L2026del DBIA    Probe Info 
HCT116 Deletion: p.I351SfsTer23;p.L1905FfsTer30
SNV: p.G1075W;p.R2243C		 DBIA    Probe Info 
HCT15 SNV: p.L190M .
HEC1B SNV: p.R286H; p.R2163H .
HT115 SNV: p.R463W; p.F668S .
IGROV1 SNV: p.E597D DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER SNV: p.F988C DBIA    Probe Info 
JURKAT SNV: p.V2045L .
JVM3 SNV: p.A470T DBIA    Probe Info 
L428 SNV: p.Q1700Ter DBIA    Probe Info 
LNCaP clone FGC SNV: p.D1426G DBIA    Probe Info 
MDAMB453 SNV: p.E2027K .
MELJUSO SNV: p.P2345L DBIA    Probe Info 
MEWO SNV: p.R546C DBIA    Probe Info 
MFE319 SNV: p.Y675C; p.T976I; p.A1127V .
NCIH1299 SNV: p.R1337Q .
NCIH2172 SNV: p.D1139G .
OVCAR5 SNV: p.R1443C DBIA    Probe Info 
TE11 Deletion: p.S389_Q392del DBIA    Probe Info 
TYKNU SNV: p.W1798S DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C1780(1.09)  LDD2092  [1]
AHL-Pu-1
 Probe Info  		C2378(3.06); C1780(2.59)  LDD0168  [2]
DBIA
 Probe Info  		C1116(1.41)  LDD0080  [3]
CY4
 Probe Info  		N.A.  LDD0247  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [5]
Lodoacetamide azide
 Probe Info  		C1161(0.00); C1947(0.00)  LDD0037  [6]
IPM
 Probe Info  		N.A.  LDD0005  [7]
NPM
 Probe Info  		N.A.  LDD0016  [7]
TFBX
 Probe Info  		C1980(0.00); C1655(0.00); C1780(0.00); C1116(0.00)  LDD0148  [8]
VSF
 Probe Info  		N.A.  LDD0007  [7]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [9]
Methacrolein
 Probe Info  		N.A.  LDD0218  [9]
AOyne
 Probe Info  		12.40  LDD0443  [10]
NAIA_5
 Probe Info  		C1980(0.00); C1902(0.00)  LDD2223  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C1780(0.78)  LDD2142  [1]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C1780(1.03)  LDD2112  [1]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C1780(0.67)  LDD2117  [1]
 LDCM0025  4SU-RNA HEK-293T C2378(3.06); C1780(2.59)  LDD0168  [2]
 LDCM0026  4SU-RNA+native RNA HEK-293T C2378(2.48); C1780(2.54)  LDD0169  [2]
 LDCM0214  AC1 HCT 116 C906(1.19)  LDD0531  [3]
 LDCM0215  AC10 HCT 116 C906(0.99)  LDD0532  [3]
 LDCM0216  AC100 HEK-293T C906(1.00)  LDD0814  [3]
 LDCM0217  AC101 HEK-293T C906(1.02)  LDD0815  [3]
 LDCM0218  AC102 HEK-293T C906(0.92)  LDD0816  [3]
 LDCM0219  AC103 HEK-293T C906(1.11)  LDD0817  [3]
 LDCM0220  AC104 HEK-293T C906(0.99)  LDD0818  [3]
 LDCM0221  AC105 HEK-293T C906(1.06)  LDD0819  [3]
 LDCM0222  AC106 HEK-293T C906(1.01)  LDD0820  [3]
 LDCM0223  AC107 HEK-293T C906(0.87)  LDD0821  [3]
 LDCM0224  AC108 HEK-293T C906(1.03)  LDD0822  [3]
 LDCM0225  AC109 HEK-293T C906(1.01)  LDD0823  [3]
 LDCM0226  AC11 HCT 116 C906(0.95)  LDD0543  [3]
 LDCM0227  AC110 HEK-293T C906(0.97)  LDD0825  [3]
 LDCM0228  AC111 HEK-293T C906(0.93)  LDD0826  [3]
 LDCM0229  AC112 HEK-293T C906(1.02)  LDD0827  [3]
 LDCM0230  AC113 HCT 116 C906(1.10)  LDD0547  [3]
 LDCM0231  AC114 HCT 116 C906(1.10)  LDD0548  [3]
 LDCM0232  AC115 HCT 116 C906(0.99)  LDD0549  [3]
 LDCM0233  AC116 HCT 116 C906(1.03)  LDD0550  [3]
 LDCM0234  AC117 HCT 116 C906(0.95)  LDD0551  [3]
 LDCM0235  AC118 HCT 116 C906(1.04)  LDD0552  [3]
 LDCM0236  AC119 HCT 116 C906(0.95)  LDD0553  [3]
 LDCM0237  AC12 HCT 116 C906(0.97)  LDD0554  [3]
 LDCM0238  AC120 HCT 116 C906(1.12)  LDD0555  [3]
 LDCM0239  AC121 HCT 116 C906(1.12)  LDD0556  [3]
 LDCM0240  AC122 HCT 116 C906(0.87)  LDD0557  [3]
 LDCM0241  AC123 HCT 116 C906(1.32)  LDD0558  [3]
 LDCM0242  AC124 HCT 116 C906(1.27)  LDD0559  [3]
 LDCM0243  AC125 HCT 116 C906(1.10)  LDD0560  [3]
 LDCM0244  AC126 HCT 116 C906(1.27)  LDD0561  [3]
 LDCM0245  AC127 HCT 116 C906(1.18)  LDD0562  [3]
 LDCM0246  AC128 HCT 116 C906(1.27)  LDD0563  [3]
 LDCM0247  AC129 HCT 116 C906(1.36)  LDD0564  [3]
 LDCM0249  AC130 HCT 116 C906(1.08)  LDD0566  [3]
 LDCM0250  AC131 HCT 116 C906(1.32)  LDD0567  [3]
 LDCM0251  AC132 HCT 116 C906(1.24)  LDD0568  [3]
 LDCM0252  AC133 HCT 116 C906(1.27)  LDD0569  [3]
 LDCM0253  AC134 HCT 116 C906(1.24)  LDD0570  [3]
 LDCM0254  AC135 HCT 116 C906(1.37)  LDD0571  [3]
 LDCM0255  AC136 HCT 116 C906(1.11)  LDD0572  [3]
 LDCM0256  AC137 HCT 116 C906(1.21)  LDD0573  [3]
 LDCM0257  AC138 HCT 116 C906(1.35)  LDD0574  [3]
 LDCM0258  AC139 HCT 116 C906(1.24)  LDD0575  [3]
 LDCM0259  AC14 HCT 116 C906(1.10)  LDD0576  [3]
 LDCM0260  AC140 HCT 116 C906(1.39)  LDD0577  [3]
 LDCM0261  AC141 HCT 116 C906(1.34)  LDD0578  [3]
 LDCM0262  AC142 HCT 116 C906(1.17)  LDD0579  [3]
 LDCM0263  AC143 HCT 116 C906(0.93)  LDD0580  [3]
 LDCM0264  AC144 HCT 116 C906(0.86)  LDD0581  [3]
 LDCM0265  AC145 HCT 116 C906(0.96)  LDD0582  [3]
 LDCM0266  AC146 HCT 116 C906(1.10)  LDD0583  [3]
 LDCM0267  AC147 HCT 116 C906(0.70)  LDD0584  [3]
 LDCM0268  AC148 HCT 116 C906(1.78)  LDD0585  [3]
 LDCM0269  AC149 HCT 116 C906(1.59)  LDD0586  [3]
 LDCM0270  AC15 HCT 116 C906(0.99)  LDD0587  [3]
 LDCM0271  AC150 HCT 116 C906(0.88)  LDD0588  [3]
 LDCM0272  AC151 HCT 116 C906(0.67)  LDD0589  [3]
 LDCM0273  AC152 HCT 116 C906(0.81)  LDD0590  [3]
 LDCM0274  AC153 HCT 116 C906(1.21)  LDD0591  [3]
 LDCM0621  AC154 HCT 116 C906(0.96)  LDD2158  [3]
 LDCM0622  AC155 HCT 116 C906(1.06)  LDD2159  [3]
 LDCM0623  AC156 HCT 116 C906(0.90)  LDD2160  [3]
 LDCM0624  AC157 HCT 116 C906(0.87)  LDD2161  [3]
 LDCM0276  AC17 HCT 116 C906(0.93)  LDD0593  [3]
 LDCM0277  AC18 HCT 116 C906(1.21)  LDD0594  [3]
 LDCM0278  AC19 HCT 116 C906(0.95)  LDD0595  [3]
 LDCM0279  AC2 HCT 116 C906(1.28)  LDD0596  [3]
 LDCM0280  AC20 HCT 116 C906(1.01)  LDD0597  [3]
 LDCM0281  AC21 HCT 116 C906(1.12)  LDD0598  [3]
 LDCM0282  AC22 HCT 116 C906(0.94)  LDD0599  [3]
 LDCM0283  AC23 HCT 116 C906(0.94)  LDD0600  [3]
 LDCM0284  AC24 HCT 116 C906(0.90)  LDD0601  [3]
 LDCM0285  AC25 HCT 116 C1116(0.74); C906(0.98)  LDD0602  [3]
 LDCM0286  AC26 HCT 116 C1116(0.83); C906(0.90)  LDD0603  [3]
 LDCM0287  AC27 HCT 116 C1116(0.84); C906(0.91)  LDD0604  [3]
 LDCM0288  AC28 HCT 116 C1116(0.85); C906(0.94)  LDD0605  [3]
 LDCM0289  AC29 HCT 116 C906(0.87); C1116(0.99)  LDD0606  [3]
 LDCM0290  AC3 HCT 116 C906(1.13)  LDD0607  [3]
 LDCM0291  AC30 HCT 116 C906(0.97); C1116(1.14)  LDD0608  [3]
 LDCM0292  AC31 HCT 116 C1116(0.83); C906(0.92)  LDD0609  [3]
 LDCM0293  AC32 HCT 116 C906(0.97); C1116(1.10)  LDD0610  [3]
 LDCM0294  AC33 HCT 116 C1116(0.84); C906(0.88)  LDD0611  [3]
 LDCM0295  AC34 HCT 116 C1116(0.82); C906(0.90)  LDD0612  [3]
 LDCM0296  AC35 HCT 116 C906(0.91)  LDD0613  [3]
 LDCM0297  AC36 HCT 116 C906(0.79)  LDD0614  [3]
 LDCM0298  AC37 HCT 116 C906(0.89)  LDD0615  [3]
 LDCM0299  AC38 HCT 116 C906(0.95)  LDD0616  [3]
 LDCM0300  AC39 HCT 116 C906(0.86)  LDD0617  [3]
 LDCM0301  AC4 HCT 116 C906(1.29)  LDD0618  [3]
 LDCM0302  AC40 HCT 116 C906(0.92)  LDD0619  [3]
 LDCM0303  AC41 HCT 116 C906(0.88)  LDD0620  [3]
 LDCM0304  AC42 HCT 116 C906(1.01)  LDD0621  [3]
 LDCM0305  AC43 HCT 116 C906(0.99)  LDD0622  [3]
 LDCM0306  AC44 HCT 116 C906(0.93)  LDD0623  [3]
 LDCM0307  AC45 HCT 116 C906(0.85)  LDD0624  [3]
 LDCM0308  AC46 HCT 116 C906(0.86)  LDD0625  [3]
 LDCM0309  AC47 HCT 116 C906(0.82)  LDD0626  [3]
 LDCM0310  AC48 HCT 116 C906(0.81)  LDD0627  [3]
 LDCM0311  AC49 HCT 116 C906(0.91)  LDD0628  [3]
 LDCM0312  AC5 HCT 116 C906(1.34)  LDD0629  [3]
 LDCM0313  AC50 HCT 116 C906(1.01)  LDD0630  [3]
 LDCM0314  AC51 HCT 116 C906(0.86)  LDD0631  [3]
 LDCM0315  AC52 HCT 116 C906(0.83)  LDD0632  [3]
 LDCM0316  AC53 HCT 116 C906(0.92)  LDD0633  [3]
 LDCM0317  AC54 HCT 116 C906(0.94)  LDD0634  [3]
 LDCM0318  AC55 HCT 116 C906(0.88)  LDD0635  [3]
 LDCM0319  AC56 HCT 116 C906(1.01)  LDD0636  [3]
 LDCM0320  AC57 HEK-293T C906(1.17)  LDD0918  [3]
 LDCM0321  AC58 HEK-293T C906(1.14)  LDD0919  [3]
 LDCM0322  AC59 HEK-293T C906(1.03)  LDD0920  [3]
 LDCM0323  AC6 HCT 116 C906(0.98)  LDD0640  [3]
 LDCM0324  AC60 HEK-293T C906(1.18)  LDD0922  [3]
 LDCM0325  AC61 HEK-293T C906(0.95)  LDD0923  [3]
 LDCM0326  AC62 HEK-293T C906(1.07)  LDD0924  [3]
 LDCM0327  AC63 HEK-293T C906(1.00)  LDD0925  [3]
 LDCM0328  AC64 HEK-293T C906(1.03)  LDD0926  [3]
 LDCM0329  AC65 HEK-293T C906(1.10)  LDD0927  [3]
 LDCM0330  AC66 HEK-293T C906(0.93)  LDD0928  [3]
 LDCM0331  AC67 HEK-293T C906(1.06)  LDD0929  [3]
 LDCM0332  AC68 HCT 116 C906(1.11)  LDD0649  [3]
 LDCM0333  AC69 HCT 116 C906(1.11)  LDD0650  [3]
 LDCM0334  AC7 HCT 116 C906(0.97)  LDD0651  [3]
 LDCM0335  AC70 HCT 116 C906(1.00)  LDD0652  [3]
 LDCM0336  AC71 HCT 116 C906(1.10)  LDD0653  [3]
 LDCM0337  AC72 HCT 116 C906(1.12)  LDD0654  [3]
 LDCM0338  AC73 HCT 116 C906(1.12)  LDD0655  [3]
 LDCM0339  AC74 HCT 116 C906(1.14)  LDD0656  [3]
 LDCM0340  AC75 HCT 116 C906(1.03)  LDD0657  [3]
 LDCM0341  AC76 HCT 116 C906(1.17)  LDD0658  [3]
 LDCM0342  AC77 HCT 116 C906(0.96)  LDD0659  [3]
 LDCM0343  AC78 HCT 116 C906(1.07)  LDD0660  [3]
 LDCM0344  AC79 HCT 116 C906(0.95)  LDD0661  [3]
 LDCM0345  AC8 HCT 116 C906(0.99)  LDD0662  [3]
 LDCM0346  AC80 HCT 116 C906(1.01)  LDD0663  [3]
 LDCM0347  AC81 HCT 116 C906(1.21)  LDD0664  [3]
 LDCM0348  AC82 HCT 116 C906(1.28)  LDD0665  [3]
 LDCM0349  AC83 HCT 116 C906(1.14)  LDD0666  [3]
 LDCM0350  AC84 HCT 116 C906(1.05)  LDD0667  [3]
 LDCM0351  AC85 HCT 116 C906(0.93)  LDD0668  [3]
 LDCM0352  AC86 HCT 116 C906(0.92)  LDD0669  [3]
 LDCM0353  AC87 HCT 116 C906(0.89)  LDD0670  [3]
 LDCM0354  AC88 HCT 116 C906(0.86)  LDD0671  [3]
 LDCM0355  AC89 HCT 116 C906(1.03)  LDD0672  [3]
 LDCM0357  AC90 HCT 116 C906(0.79)  LDD0674  [3]
 LDCM0358  AC91 HCT 116 C906(1.04)  LDD0675  [3]
 LDCM0359  AC92 HCT 116 C906(1.08)  LDD0676  [3]
 LDCM0360  AC93 HCT 116 C906(0.99)  LDD0677  [3]
 LDCM0361  AC94 HCT 116 C906(0.91)  LDD0678  [3]
 LDCM0362  AC95 HCT 116 C906(0.92)  LDD0679  [3]
 LDCM0363  AC96 HCT 116 C906(1.03)  LDD0680  [3]
 LDCM0364  AC97 HCT 116 C906(1.21)  LDD0681  [3]
 LDCM0365  AC98 HEK-293T C906(1.14)  LDD0963  [3]
 LDCM0366  AC99 HEK-293T C906(1.01)  LDD0964  [3]
 LDCM0248  AKOS034007472 HCT 116 C906(0.98)  LDD0565  [3]
 LDCM0356  AKOS034007680 HCT 116 C906(0.98)  LDD0673  [3]
 LDCM0275  AKOS034007705 HCT 116 C906(1.01)  LDD0592  [3]
 LDCM0367  CL1 HEK-293T C1116(1.48); C1913(1.72); C906(0.79)  LDD0965  [3]
 LDCM0368  CL10 HEK-293T C1116(2.18); C1913(1.39); C906(0.73)  LDD0966  [3]
 LDCM0369  CL100 HCT 116 C906(1.23)  LDD0686  [3]
 LDCM0370  CL101 HCT 116 C906(0.97)  LDD0687  [3]
 LDCM0371  CL102 HCT 116 C906(1.04)  LDD0688  [3]
 LDCM0372  CL103 HCT 116 C906(1.08)  LDD0689  [3]
 LDCM0373  CL104 HCT 116 C906(0.99)  LDD0690  [3]
 LDCM0374  CL105 HCT 116 C906(1.37)  LDD0691  [3]
 LDCM0375  CL106 HCT 116 C906(1.65)  LDD0692  [3]
 LDCM0376  CL107 HCT 116 C906(1.34)  LDD0693  [3]
 LDCM0377  CL108 HCT 116 C906(1.03)  LDD0694  [3]
 LDCM0378  CL109 HCT 116 C906(1.40)  LDD0695  [3]
 LDCM0379  CL11 HEK-293T C1116(2.14); C1913(1.54); C906(0.86)  LDD0977  [3]
 LDCM0380  CL110 HCT 116 C906(1.16)  LDD0697  [3]
 LDCM0381  CL111 HCT 116 C906(0.95)  LDD0698  [3]
 LDCM0382  CL112 HCT 116 C906(0.91); C1116(0.91)  LDD0699  [3]
 LDCM0383  CL113 HCT 116 C1116(0.91); C906(0.94)  LDD0700  [3]
 LDCM0384  CL114 HCT 116 C906(0.85); C1116(0.94)  LDD0701  [3]
 LDCM0385  CL115 HCT 116 C906(0.90); C1116(1.00)  LDD0702  [3]
 LDCM0386  CL116 HCT 116 C1116(0.83); C906(0.94)  LDD0703  [3]
 LDCM0387  CL117 HCT 116 C906(1.17)  LDD0704  [3]
 LDCM0388  CL118 HCT 116 C906(0.94)  LDD0705  [3]
 LDCM0389  CL119 HCT 116 C906(0.99)  LDD0706  [3]
 LDCM0390  CL12 HEK-293T C1116(1.18); C1913(1.19); C906(1.06)  LDD0988  [3]
 LDCM0391  CL120 HCT 116 C906(0.82)  LDD0708  [3]
 LDCM0392  CL121 HCT 116 C906(0.87)  LDD0709  [3]
 LDCM0393  CL122 HCT 116 C906(1.09)  LDD0710  [3]
 LDCM0394  CL123 HCT 116 C906(0.98)  LDD0711  [3]
 LDCM0395  CL124 HCT 116 C906(0.85)  LDD0712  [3]
 LDCM0396  CL125 HEK-293T C906(0.87)  LDD0994  [3]
 LDCM0397  CL126 HEK-293T C906(1.05)  LDD0995  [3]
 LDCM0398  CL127 HEK-293T C906(0.92)  LDD0996  [3]
 LDCM0399  CL128 HEK-293T C906(0.83)  LDD0997  [3]
 LDCM0400  CL13 HEK-293T C1116(1.16); C1913(1.33); C906(0.98)  LDD0998  [3]
 LDCM0401  CL14 HEK-293T C1116(1.40); C1913(1.55); C906(0.86)  LDD0999  [3]
 LDCM0402  CL15 HEK-293T C1116(1.72); C1913(1.52); C906(0.79)  LDD1000  [3]
 LDCM0403  CL16 HCT 116 C906(0.98)  LDD0720  [3]
 LDCM0404  CL17 HCT 116 C906(1.07); C1116(1.09)  LDD0721  [3]
 LDCM0405  CL18 HCT 116 C906(0.89); C1116(0.99)  LDD0722  [3]
 LDCM0406  CL19 HCT 116 C1116(0.75); C906(1.00)  LDD0723  [3]
 LDCM0407  CL2 HEK-293T C1116(1.33); C1913(1.79); C906(0.72)  LDD1005  [3]
 LDCM0408  CL20 HCT 116 C906(0.98); C1116(1.10)  LDD0725  [3]
 LDCM0409  CL21 HCT 116 C1116(0.83); C906(1.03)  LDD0726  [3]
 LDCM0410  CL22 HCT 116 C1116(0.78); C906(1.12)  LDD0727  [3]
 LDCM0411  CL23 HCT 116 C906(1.02); C1116(1.26)  LDD0728  [3]
 LDCM0412  CL24 HCT 116 C1116(0.86); C906(1.02)  LDD0729  [3]
 LDCM0413  CL25 HCT 116 C1116(0.75); C906(1.02)  LDD0730  [3]
 LDCM0414  CL26 HCT 116 C1116(0.96); C906(0.97)  LDD0731  [3]
 LDCM0415  CL27 HCT 116 C1116(1.21); C906(0.91)  LDD0732  [3]
 LDCM0416  CL28 HCT 116 C1116(0.82); C906(0.97)  LDD0733  [3]
 LDCM0417  CL29 HCT 116 C1116(0.81); C906(0.99)  LDD0734  [3]
 LDCM0418  CL3 HEK-293T C1116(1.30); C1913(1.09); C906(1.24)  LDD1016  [3]
 LDCM0419  CL30 HCT 116 C1116(0.94); C906(1.00)  LDD0736  [3]
 LDCM0420  CL31 HCT 116 C1116(0.84)  LDD0737  [3]
 LDCM0421  CL32 HEK-293T C1913(0.81); C906(1.13)  LDD1019  [3]
 LDCM0422  CL33 HEK-293T C1913(1.35); C906(1.25)  LDD1020  [3]
 LDCM0423  CL34 HEK-293T C1913(1.40); C906(1.19)  LDD1021  [3]
 LDCM0424  CL35 HEK-293T C1913(0.92); C906(1.22)  LDD1022  [3]
 LDCM0425  CL36 HEK-293T C1913(0.99); C906(1.08)  LDD1023  [3]
 LDCM0426  CL37 HEK-293T C1913(1.04); C906(1.15)  LDD1024  [3]
 LDCM0428  CL39 HEK-293T C1913(1.32); C906(1.56)  LDD1026  [3]
 LDCM0429  CL4 HEK-293T C1116(1.37); C1913(1.24); C906(0.57)  LDD1027  [3]
 LDCM0430  CL40 HEK-293T C1913(1.21); C906(1.18)  LDD1028  [3]
 LDCM0431  CL41 HEK-293T C1913(1.16); C906(1.33)  LDD1029  [3]
 LDCM0432  CL42 HEK-293T C1913(0.84); C906(1.35)  LDD1030  [3]
 LDCM0433  CL43 HEK-293T C1913(1.12); C906(1.40)  LDD1031  [3]
 LDCM0434  CL44 HEK-293T C1913(0.95); C906(1.09)  LDD1032  [3]
 LDCM0435  CL45 HEK-293T C1913(1.37); C906(1.37)  LDD1033  [3]
 LDCM0436  CL46 HCT 116 C906(0.76)  LDD0753  [3]
 LDCM0437  CL47 HCT 116 C906(0.77)  LDD0754  [3]
 LDCM0438  CL48 HCT 116 C906(0.74)  LDD0755  [3]
 LDCM0439  CL49 HCT 116 C906(0.84)  LDD0756  [3]
 LDCM0440  CL5 HEK-293T C1116(1.34); C1913(1.00); C906(0.78)  LDD1038  [3]
 LDCM0441  CL50 HCT 116 C906(0.82)  LDD0758  [3]
 LDCM0442  CL51 HCT 116 C906(0.80)  LDD0759  [3]
 LDCM0443  CL52 HCT 116 C906(0.76)  LDD0760  [3]
 LDCM0444  CL53 HCT 116 C906(0.79)  LDD0761  [3]
 LDCM0445  CL54 HCT 116 C906(0.72)  LDD0762  [3]
 LDCM0446  CL55 HCT 116 C906(0.72)  LDD0763  [3]
 LDCM0447  CL56 HCT 116 C906(0.90)  LDD0764  [3]
 LDCM0448  CL57 HCT 116 C906(0.76)  LDD0765  [3]
 LDCM0449  CL58 HCT 116 C906(0.77)  LDD0766  [3]
 LDCM0450  CL59 HCT 116 C906(0.95)  LDD0767  [3]
 LDCM0451  CL6 HEK-293T C1116(1.42); C1913(1.72); C906(0.80)  LDD1049  [3]
 LDCM0452  CL60 HCT 116 C906(0.88)  LDD0769  [3]
 LDCM0453  CL61 HCT 116 C1116(0.92); C906(1.29)  LDD0770  [3]
 LDCM0454  CL62 HCT 116 C1116(0.86); C906(1.25)  LDD0771  [3]
 LDCM0455  CL63 HCT 116 C1116(0.83); C906(1.10)  LDD0772  [3]
 LDCM0456  CL64 HCT 116 C1116(0.86); C906(1.12)  LDD0773  [3]
 LDCM0457  CL65 HCT 116 C1116(0.89); C906(1.12)  LDD0774  [3]
 LDCM0458  CL66 HCT 116 C1116(0.97); C906(1.50)  LDD0775  [3]
 LDCM0459  CL67 HCT 116 C1116(0.96); C906(1.41)  LDD0776  [3]
 LDCM0460  CL68 HCT 116 C1116(1.12); C906(1.43)  LDD0777  [3]
 LDCM0461  CL69 HCT 116 C1116(0.83); C906(1.47)  LDD0778  [3]
 LDCM0462  CL7 HEK-293T C1116(1.43); C1913(1.10); C906(1.24)  LDD1060  [3]
 LDCM0463  CL70 HCT 116 C1116(0.94); C906(1.35)  LDD0780  [3]
 LDCM0464  CL71 HCT 116 C1116(0.89); C906(1.39)  LDD0781  [3]
 LDCM0465  CL72 HCT 116 C1116(0.97); C906(1.08)  LDD0782  [3]
 LDCM0466  CL73 HCT 116 C1116(0.94); C906(1.36)  LDD0783  [3]
 LDCM0467  CL74 HCT 116 C1116(0.91); C906(1.25)  LDD0784  [3]
 LDCM0469  CL76 HCT 116 C1116(0.90)  LDD0786  [3]
 LDCM0470  CL77 HCT 116 C1116(1.00)  LDD0787  [3]
 LDCM0471  CL78 HCT 116 C1116(0.97)  LDD0788  [3]
 LDCM0472  CL79 HCT 116 C1116(1.13)  LDD0789  [3]
 LDCM0473  CL8 HEK-293T C1116(1.60); C906(0.87)  LDD1071  [3]
 LDCM0474  CL80 HCT 116 C1116(1.00)  LDD0791  [3]
 LDCM0475  CL81 HCT 116 C1116(0.87)  LDD0792  [3]
 LDCM0476  CL82 HCT 116 C1116(0.94)  LDD0793  [3]
 LDCM0477  CL83 HCT 116 C1116(0.93)  LDD0794  [3]
 LDCM0478  CL84 HCT 116 C1116(0.97)  LDD0795  [3]
 LDCM0479  CL85 HCT 116 C1116(1.09)  LDD0796  [3]
 LDCM0480  CL86 HCT 116 C1116(0.94)  LDD0797  [3]
 LDCM0481  CL87 HCT 116 C1116(0.96)  LDD0798  [3]
 LDCM0482  CL88 HCT 116 C1116(0.92)  LDD0799  [3]
 LDCM0483  CL89 HCT 116 C1116(0.92)  LDD0800  [3]
 LDCM0484  CL9 HEK-293T C1116(1.32); C906(0.81)  LDD1082  [3]
 LDCM0485  CL90 HCT 116 C1116(1.13)  LDD0802  [3]
 LDCM0486  CL91 HCT 116 C906(1.17)  LDD0803  [3]
 LDCM0487  CL92 HCT 116 C906(1.26)  LDD0804  [3]
 LDCM0488  CL93 HCT 116 C906(1.06)  LDD0805  [3]
 LDCM0489  CL94 HCT 116 C906(1.44)  LDD0806  [3]
 LDCM0490  CL95 HCT 116 C906(1.46)  LDD0807  [3]
 LDCM0491  CL96 HCT 116 C906(1.21)  LDD0808  [3]
 LDCM0492  CL97 HCT 116 C906(1.23)  LDD0809  [3]
 LDCM0493  CL98 HCT 116 C906(1.13)  LDD0810  [3]
 LDCM0494  CL99 HCT 116 C906(1.34)  LDD0811  [3]
 LDCM0495  E2913 HEK-293T C1691(0.99); C1913(0.90); C2300(0.94); C1780(1.02)  LDD1698  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C1780(0.74)  LDD1702  [1]
 LDCM0468  Fragment33 HCT 116 C1116(0.85); C906(1.15)  LDD0785  [3]
 LDCM0427  Fragment51 HEK-293T C1913(0.94); C906(1.09)  LDD1025  [3]
 LDCM0022  KB02 HCT 116 C1116(1.41)  LDD0080  [3]
 LDCM0023  KB03 HCT 116 C1116(1.63)  LDD0081  [3]
 LDCM0024  KB05 HCT 116 C1116(2.67)  LDD0082  [3]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C1780(1.09)  LDD2092  [1]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C1780(1.41)  LDD2098  [1]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C1780(1.24)  LDD2099  [1]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C1780(0.74)  LDD2104  [1]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C1780(1.17)  LDD2105  [1]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C1780(0.85)  LDD2108  [1]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C1780(0.66)  LDD2114  [1]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C1780(0.99)  LDD2123  [1]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C1780(1.66)  LDD2136  [1]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C1780(1.11)  LDD2140  [1]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C1780(4.11)  LDD2145  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Chromodomain-helicase-DNA-binding protein 7 (CHD7) SNF2/RAD54 helicase family Q9P2D1
Cytosolic acyl coenzyme A thioester hydrolase (ACOT7) . O00154
Set1/Ash2 histone methyltransferase complex subunit ASH2 (ASH2L) . Q9UBL3
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and BTB domain-containing protein 7A (ZBTB7A) . O95365
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
WD repeat-containing protein 5 (WDR5) WD repeat WDR5/wds family P61964
Retinoblastoma-binding protein 5 (RBBP5) . Q15291

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402