Details of the Target

General Information of Target

Target ID LDTP10033
Target Name WW domain-binding protein 2 (WBP2)
Gene Name WBP2
Gene ID 23558
Synonyms
WW domain-binding protein 2; WBP-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEEVSTLMKATVLMRQPGRVQEIVGALRKGGGDRLQVISDFDMTLSRFAYNGKRCPSSY
NILDNSKIISEECRKELTALLHHYYPIEIDPHRTVKEKLPHMVEWWTKAHNLLCQQKIQK
FQIAQVVRESNAMLREGYKTFFNTLYHNNIPLFIFSAGIGDILEEIIRQMKVFHPNIHIV
SNYMDFNEDGFLQGFKGQLIHTYNKNSSACENSGYFQQLEGKTNVILLGDSIGDLTMADG
VPGVQNILKIGFLNDKVEERRERYMDSYDIVLEKDETLDVVNGLLQHILCQGVQLEMQGP
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Acts as a transcriptional coactivator of estrogen and progesterone receptors (ESR1 and PGR) upon hormone activation. In presence of estrogen, binds to ESR1-responsive promoters. Required for YAP1 coactivation function on PGR activity. Synergizes with WBP2 in enhancing PGR activity. Modulates expression of post-synaptic scaffolding proteins via regulation of ESR1, ESR2 and PGR.
Uniprot ID
Q969T9
Ensemble ID
ENST00000254806.8
HGNC ID
HGNC:12738

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JURKAT SNV: p.G88S Compound 10    Probe Info 
MM1S SNV: p.A98V .
RCC10RGB SNV: p.V50I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 18 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TH211
 Probe Info  		Y91(20.00)  LDD0260  [2]
STPyne
 Probe Info  		K47(2.27); K62(0.43); K83(7.10)  LDD0277  [3]
Probe 1
 Probe Info  		Y55(17.30)  LDD3495  [4]
WP
 Probe Info  		2.79  LDD0192  [5]
AHL-Pu-1
 Probe Info  		C80(4.52)  LDD0168  [6]
HHS-482
 Probe Info  		Y55(1.13); Y91(1.30)  LDD0285  [7]
HHS-475
 Probe Info  		Y91(0.93); Y231(0.93); Y252(0.96); Y55(0.97)  LDD0264  [8]
HHS-465
 Probe Info  		Y231(2.06); Y241(7.82); Y55(10.00); Y91(10.00)  LDD2237  [9]
ATP probe
 Probe Info  		K47(0.00); K62(0.00); K64(0.00)  LDD0199  [10]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [11]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [12]
NAIA_4
 Probe Info  		N.A.  LDD2226  [13]
Compound 10
 Probe Info  		N.A.  LDD2216  [14]
Compound 11
 Probe Info  		N.A.  LDD2213  [14]
IPM
 Probe Info  		N.A.  LDD0147  [15]
TFBX
 Probe Info  		N.A.  LDD0148  [15]
NAIA_5
 Probe Info  		N.A.  LDD2223  [13]
PAL-AfBPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C218
 Probe Info  		11.24  LDD1892  [16]
C220
 Probe Info  		23.26  LDD1894  [16]
C310
 Probe Info  		9.25  LDD1977  [16]
FFF probe13
 Probe Info  		10.68  LDD0475  [17]
FFF probe2
 Probe Info  		14.55  LDD0463  [17]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C80(4.52)  LDD0168  [6]
 LDCM0026  4SU-RNA+native RNA HEK-293T C80(11.75)  LDD0169  [6]
 LDCM0156  Aniline NCI-H1299 12.83  LDD0403  [1]
 LDCM0632  CL-Sc Hep-G2 C80(0.84); C80(0.78)  LDD2227  [13]
 LDCM0625  F8 Ramos C80(1.43)  LDD2187  [18]
 LDCM0572  Fragment10 Ramos C80(1.04)  LDD2189  [18]
 LDCM0574  Fragment12 Ramos C80(1.11)  LDD2191  [18]
 LDCM0575  Fragment13 Ramos C80(1.73)  LDD2192  [18]
 LDCM0576  Fragment14 Ramos C80(0.90)  LDD2193  [18]
 LDCM0579  Fragment20 Ramos C80(0.99)  LDD2194  [18]
 LDCM0580  Fragment21 Ramos C80(1.71)  LDD2195  [18]
 LDCM0582  Fragment23 Ramos C80(1.34)  LDD2196  [18]
 LDCM0578  Fragment27 Ramos C80(1.85)  LDD2197  [18]
 LDCM0586  Fragment28 Ramos C80(0.53)  LDD2198  [18]
 LDCM0588  Fragment30 Ramos C80(1.21)  LDD2199  [18]
 LDCM0589  Fragment31 Ramos C80(1.46)  LDD2200  [18]
 LDCM0590  Fragment32 Ramos C80(1.80)  LDD2201  [18]
 LDCM0468  Fragment33 Ramos C80(1.68)  LDD2202  [18]
 LDCM0596  Fragment38 Ramos C80(1.48)  LDD2203  [18]
 LDCM0566  Fragment4 Ramos C80(0.93)  LDD2184  [18]
 LDCM0610  Fragment52 Ramos C80(1.54)  LDD2204  [18]
 LDCM0614  Fragment56 Ramos C80(1.53)  LDD2205  [18]
 LDCM0569  Fragment7 Ramos C80(0.92)  LDD2186  [18]
 LDCM0571  Fragment9 Ramos C80(1.47)  LDD2188  [18]
 LDCM0116  HHS-0101 DM93 Y91(0.93); Y231(0.93); Y252(0.96); Y55(0.97)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y75(0.44); Y253(0.47); Y91(0.83); Y55(0.84)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y91(0.68); Y252(0.81); Y75(0.85); Y253(0.86)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y253(0.67); Y55(0.68); Y231(0.71); Y75(0.84)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y253(0.70); Y75(0.91); Y91(1.00); Y231(1.07)  LDD0268  [8]
 LDCM0123  JWB131 DM93 Y55(1.13); Y91(1.30)  LDD0285  [7]
 LDCM0124  JWB142 DM93 Y231(0.44); Y232(0.52); Y55(0.67); Y91(1.35)  LDD0286  [7]
 LDCM0125  JWB146 DM93 Y231(1.75); Y232(2.89); Y91(1.32)  LDD0287  [7]
 LDCM0126  JWB150 DM93 Y231(2.78); Y232(2.29); Y91(5.61)  LDD0288  [7]
 LDCM0127  JWB152 DM93 Y232(4.22); Y55(1.44); Y91(3.70)  LDD0289  [7]
 LDCM0128  JWB198 DM93 Y231(1.22); Y232(1.88); Y91(2.23)  LDD0290  [7]
 LDCM0129  JWB202 DM93 Y231(1.28); Y232(1.36); Y55(0.43); Y91(1.48)  LDD0291  [7]
 LDCM0130  JWB211 DM93 Y231(0.91); Y232(0.91); Y55(0.85); Y91(1.72)  LDD0292  [7]
 LDCM0022  KB02 Ramos C80(0.96)  LDD2182  [18]
 LDCM0023  KB03 Jurkat C80(4.80)  LDD0315  [11]
 LDCM0024  KB05 Ramos C80(0.95)  LDD2185  [18]
 LDCM0131  RA190 MM1.R C80(1.28)  LDD0304  [19]
 LDCM0085  WA NCI-H1975 2.79  LDD0192  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin-conjugating enzyme E2 E3 (UBE2E3) Ubiquitin-conjugating enzyme family Q969T4
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mothers against decapentaplegic homolog 5 (SMAD5) Dwarfin/SMAD family Q99717
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homer protein homolog 3 (HOMER3) Homer family Q9NSC5
T-complex protein 1 subunit eta (CCT7) TCP-1 chaperonin family Q99832
Transcriptional coactivator YAP1 (YAP1) YAP1 family P46937
BAG family molecular chaperone regulator 3 (BAG3) . O95817
WW domain-containing transcription regulator protein 1 (WWTR1) . Q9GZV5

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 Identification of peroxiredoxin 6 as a direct target of withangulatin A by quantitative chemical proteomics in non-small cell lung cancer. Redox Biol. 2021 Oct;46:102130. doi: 10.1016/j.redox.2021.102130. Epub 2021 Sep 9.
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
12 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
13 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
14 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
15 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
16 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
17 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
18 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
19 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.