Details of the Target

General Information of Target

Target ID LDTP05967
Target Name Nucleic acid dioxygenase ALKBH1 (ALKBH1)
Gene Name ALKBH1
Gene ID 8846
Synonyms
ABH; ABH1; ALKBH; Nucleic acid dioxygenase ALKBH1; EC 1.14.11.-; Alkylated DNA repair protein alkB homolog 1; Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA 6mA demethylase; DNA N6-methyl adenine demethylase ALKBH1; EC 1.14.11.51; DNA lyase ABH1; EC 4.2.99.18; DNA oxidative demethylase ALKBH1; EC 1.14.11.33; mRNA N(3)-methylcytidine demethylase; EC 1.14.11.-
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGKMAAAVGSVATLATEPGEDAFRKLFRFYRQSRPGTADLEGVIDFSAAHAARGKGPGAQ
KVIKSQLNVSSVSEQNAYRAGLQPVSKWQAYGLKGYPGFIFIPNPFLPGYQWHWVKQCLK
LYSQKPNVCNLDKHMSKEETQDLWEQSKEFLRYKEATKRRPRSLLEKLRWVTVGYHYNWD
SKKYSADHYTPFPSDLGFLSEQVAAACGFEDFRAEAGILNYYRLDSTLGIHVDRSELDHS
KPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAVPRVLPNPEGEG
LPHCLEAPLPAVLPRDSMVEPCSMEDWQVCASYLKTARVNMTVRQVLATDQNFPLEPIED
EKRDISTEGFCHLDDQNSEVKRARINPDS
Target Bioclass
Enzyme
Family
AlkB family
Subcellular location
Nucleus
Function
Dioxygenase that acts as on nucleic acids, such as DNA and tRNA. Requires molecular oxygen, alpha-ketoglutarate and iron. A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment. Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs. Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation. In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation. Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA. N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing. Demethylates mRNAs containing N(3)-methylcytidine modification. Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity. Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketboglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product. DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation.
Uniprot ID
Q13686
Ensemble ID
ENST00000216489.8
HGNC ID
HGNC:17911
ChEMBL ID
CHEMBL5169151

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Alkylaryl probe 2
 Probe Info  		20.00  LDD0389  [1]
STPyne
 Probe Info  		K55(7.88)  LDD0277  [2]
NAIA_5
 Probe Info  		C371(0.52)  LDD2227  [3]
DBIA
 Probe Info  		C129(1.12); C322(0.64); C330(0.64)  LDD0532  [4]
5E-2FA
 Probe Info  		N.A.  LDD2235  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [7]
IPM
 Probe Info  		N.A.  LDD2156  [8]
VSF
 Probe Info  		N.A.  LDD0007  [9]
AOyne
 Probe Info  		13.00  LDD0443  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 PaTu 8988t C371(0.56)  LDD1093  [4]
 LDCM0215  AC10 HCT 116 C129(1.12); C322(0.64); C330(0.64)  LDD0532  [4]
 LDCM0216  AC100 HCT 116 C129(0.95)  LDD0533  [4]
 LDCM0217  AC101 HCT 116 C129(0.88)  LDD0534  [4]
 LDCM0218  AC102 HCT 116 C129(0.90)  LDD0535  [4]
 LDCM0219  AC103 HCT 116 C129(0.96)  LDD0536  [4]
 LDCM0220  AC104 HCT 116 C129(0.79)  LDD0537  [4]
 LDCM0221  AC105 HCT 116 C129(0.85)  LDD0538  [4]
 LDCM0222  AC106 HCT 116 C129(0.93)  LDD0539  [4]
 LDCM0223  AC107 HCT 116 C129(0.89)  LDD0540  [4]
 LDCM0224  AC108 HCT 116 C129(0.77)  LDD0541  [4]
 LDCM0225  AC109 HCT 116 C129(0.82)  LDD0542  [4]
 LDCM0226  AC11 HCT 116 C129(1.09); C322(0.52); C330(0.52)  LDD0543  [4]
 LDCM0227  AC110 HCT 116 C129(0.85)  LDD0544  [4]
 LDCM0228  AC111 HCT 116 C129(0.92)  LDD0545  [4]
 LDCM0229  AC112 HCT 116 C129(0.87)  LDD0546  [4]
 LDCM0237  AC12 HCT 116 C129(1.26); C322(0.90); C330(0.90)  LDD0554  [4]
 LDCM0246  AC128 HCT 116 C322(1.14); C330(1.14)  LDD0563  [4]
 LDCM0247  AC129 HCT 116 C322(1.13); C330(1.13)  LDD0564  [4]
 LDCM0249  AC130 HCT 116 C322(1.25); C330(1.25)  LDD0566  [4]
 LDCM0250  AC131 HCT 116 C322(1.23); C330(1.23)  LDD0567  [4]
 LDCM0251  AC132 HCT 116 C322(1.00); C330(1.00)  LDD0568  [4]
 LDCM0252  AC133 HCT 116 C322(1.05); C330(1.05)  LDD0569  [4]
 LDCM0253  AC134 HCT 116 C322(0.99); C330(0.99)  LDD0570  [4]
 LDCM0254  AC135 HCT 116 C322(0.99); C330(0.99)  LDD0571  [4]
 LDCM0255  AC136 HCT 116 C322(1.07); C330(1.07)  LDD0572  [4]
 LDCM0256  AC137 HCT 116 C322(0.84); C330(0.84)  LDD0573  [4]
 LDCM0257  AC138 HCT 116 C322(1.12); C330(1.12)  LDD0574  [4]
 LDCM0258  AC139 HCT 116 C322(0.87); C330(0.87)  LDD0575  [4]
 LDCM0259  AC14 HCT 116 C129(1.19); C322(0.51); C330(0.51)  LDD0576  [4]
 LDCM0260  AC140 HCT 116 C322(0.93); C330(0.93)  LDD0577  [4]
 LDCM0261  AC141 HCT 116 C322(0.97); C330(0.97)  LDD0578  [4]
 LDCM0262  AC142 HCT 116 C322(1.04); C330(1.04)  LDD0579  [4]
 LDCM0263  AC143 HCT 116 C129(1.22)  LDD0580  [4]
 LDCM0264  AC144 HCT 116 C129(0.76)  LDD0581  [4]
 LDCM0265  AC145 HCT 116 C129(0.77)  LDD0582  [4]
 LDCM0266  AC146 HCT 116 C129(0.86)  LDD0583  [4]
 LDCM0267  AC147 HCT 116 C129(0.70)  LDD0584  [4]
 LDCM0268  AC148 HCT 116 C129(0.98)  LDD0585  [4]
 LDCM0269  AC149 HCT 116 C129(1.15)  LDD0586  [4]
 LDCM0270  AC15 HCT 116 C322(0.67); C330(0.67); C129(1.48)  LDD0587  [4]
 LDCM0271  AC150 HCT 116 C129(0.98)  LDD0588  [4]
 LDCM0272  AC151 HCT 116 C129(0.78)  LDD0589  [4]
 LDCM0273  AC152 HCT 116 C129(0.95)  LDD0590  [4]
 LDCM0274  AC153 HCT 116 C129(1.17)  LDD0591  [4]
 LDCM0621  AC154 HCT 116 C129(0.94)  LDD2158  [4]
 LDCM0622  AC155 HCT 116 C129(1.04)  LDD2159  [4]
 LDCM0623  AC156 HCT 116 C129(0.91)  LDD2160  [4]
 LDCM0624  AC157 HCT 116 C129(0.85)  LDD2161  [4]
 LDCM0276  AC17 HEK-293T C129(0.77); C371(1.13); C304(0.82)  LDD1515  [11]
 LDCM0277  AC18 HEK-293T C129(0.96); C371(1.04); C304(1.03)  LDD1516  [11]
 LDCM0278  AC19 HEK-293T C129(1.08); C371(0.87); C304(0.90)  LDD1517  [11]
 LDCM0279  AC2 PaTu 8988t C371(0.55)  LDD1158  [4]
 LDCM0280  AC20 HEK-293T C129(0.92); C371(1.24); C304(0.86)  LDD1519  [11]
 LDCM0281  AC21 HEK-293T C129(1.01); C371(1.07); C304(0.87)  LDD1520  [11]
 LDCM0282  AC22 HEK-293T C129(0.94); C371(1.23)  LDD1521  [11]
 LDCM0283  AC23 HEK-293T C129(0.96); C371(1.09); C304(1.30)  LDD1522  [11]
 LDCM0284  AC24 HEK-293T C129(0.96); C371(1.19)  LDD1523  [11]
 LDCM0285  AC25 PaTu 8988t C371(1.06)  LDD1164  [4]
 LDCM0286  AC26 PaTu 8988t C371(0.97)  LDD1165  [4]
 LDCM0287  AC27 PaTu 8988t C371(1.34)  LDD1166  [4]
 LDCM0288  AC28 PaTu 8988t C371(1.55)  LDD1167  [4]
 LDCM0289  AC29 PaTu 8988t C371(1.16)  LDD1168  [4]
 LDCM0290  AC3 PaTu 8988t C371(0.63)  LDD1169  [4]
 LDCM0291  AC30 PaTu 8988t C371(0.91)  LDD1170  [4]
 LDCM0292  AC31 PaTu 8988t C371(1.02)  LDD1171  [4]
 LDCM0293  AC32 PaTu 8988t C371(0.74)  LDD1172  [4]
 LDCM0294  AC33 PaTu 8988t C371(1.12)  LDD1173  [4]
 LDCM0295  AC34 PaTu 8988t C371(0.72)  LDD1174  [4]
 LDCM0296  AC35 HCT 116 C129(1.07)  LDD0613  [4]
 LDCM0297  AC36 HCT 116 C129(1.10)  LDD0614  [4]
 LDCM0298  AC37 HCT 116 C129(1.00)  LDD0615  [4]
 LDCM0299  AC38 HCT 116 C129(1.02)  LDD0616  [4]
 LDCM0300  AC39 HCT 116 C129(0.97)  LDD0617  [4]
 LDCM0301  AC4 PaTu 8988t C371(0.55)  LDD1180  [4]
 LDCM0302  AC40 HCT 116 C129(0.93)  LDD0619  [4]
 LDCM0303  AC41 HCT 116 C129(0.77)  LDD0620  [4]
 LDCM0304  AC42 HCT 116 C129(0.81)  LDD0621  [4]
 LDCM0305  AC43 HCT 116 C129(0.89)  LDD0622  [4]
 LDCM0306  AC44 HCT 116 C129(0.89)  LDD0623  [4]
 LDCM0307  AC45 HCT 116 C129(0.79)  LDD0624  [4]
 LDCM0308  AC46 HEK-293T C129(0.93); C371(1.07)  LDD1547  [11]
 LDCM0309  AC47 HEK-293T C129(0.98); C371(1.15); C304(1.17)  LDD1548  [11]
 LDCM0310  AC48 HEK-293T C129(1.00); C371(1.07)  LDD1549  [11]
 LDCM0311  AC49 HEK-293T C129(0.98); C371(1.12); C304(1.03)  LDD1550  [11]
 LDCM0312  AC5 PaTu 8988t C371(0.48)  LDD1191  [4]
 LDCM0313  AC50 HEK-293T C129(1.03); C371(1.12); C304(1.15)  LDD1552  [11]
 LDCM0314  AC51 HEK-293T C129(1.06); C371(1.10); C304(0.87)  LDD1553  [11]
 LDCM0315  AC52 HEK-293T C129(1.00); C371(1.12); C304(0.82)  LDD1554  [11]
 LDCM0316  AC53 HEK-293T C129(1.12); C371(1.16); C304(1.08)  LDD1555  [11]
 LDCM0317  AC54 HEK-293T C129(1.04); C371(1.18)  LDD1556  [11]
 LDCM0318  AC55 HEK-293T C129(1.05); C371(1.29); C304(1.06)  LDD1557  [11]
 LDCM0319  AC56 HEK-293T C129(0.94); C371(1.02)  LDD1558  [11]
 LDCM0320  AC57 HEK-293T C129(0.86); C371(1.06); C304(0.87)  LDD1559  [11]
 LDCM0321  AC58 HEK-293T C129(1.00); C371(1.15); C304(1.02)  LDD1560  [11]
 LDCM0322  AC59 HEK-293T C129(1.10); C371(1.08); C304(1.12)  LDD1561  [11]
 LDCM0323  AC6 HCT 116 C322(0.63); C330(0.63); C129(1.07)  LDD0640  [4]
 LDCM0324  AC60 HEK-293T C129(0.90); C371(1.10); C304(1.30)  LDD1563  [11]
 LDCM0325  AC61 HEK-293T C129(0.99); C371(1.01); C304(0.87)  LDD1564  [11]
 LDCM0326  AC62 HEK-293T C129(0.95); C371(1.03)  LDD1565  [11]
 LDCM0327  AC63 HEK-293T C129(0.97); C371(1.12); C304(1.16)  LDD1566  [11]
 LDCM0328  AC64 HEK-293T C129(0.88); C371(1.01)  LDD1567  [11]
 LDCM0332  AC68 HCT 116 C322(0.65); C330(0.65)  LDD0649  [4]
 LDCM0333  AC69 HCT 116 C322(0.63); C330(0.63)  LDD0650  [4]
 LDCM0334  AC7 HCT 116 C322(0.72); C330(0.72); C129(0.95)  LDD0651  [4]
 LDCM0335  AC70 HCT 116 C322(0.43); C330(0.43)  LDD0652  [4]
 LDCM0336  AC71 HCT 116 C322(0.75); C330(0.75)  LDD0653  [4]
 LDCM0337  AC72 HCT 116 C322(0.76); C330(0.76)  LDD0654  [4]
 LDCM0338  AC73 HCT 116 C322(0.72); C330(0.72)  LDD0655  [4]
 LDCM0339  AC74 HCT 116 C322(0.52); C330(0.52)  LDD0656  [4]
 LDCM0340  AC75 HCT 116 C322(0.61); C330(0.61)  LDD0657  [4]
 LDCM0341  AC76 HCT 116 C322(0.62); C330(0.62)  LDD0658  [4]
 LDCM0342  AC77 HCT 116 C322(0.74); C330(0.74)  LDD0659  [4]
 LDCM0343  AC78 HCT 116 C322(0.82); C330(0.82)  LDD0660  [4]
 LDCM0344  AC79 HCT 116 C322(0.76); C330(0.76)  LDD0661  [4]
 LDCM0345  AC8 HCT 116 C322(0.78); C330(0.78); C129(1.28)  LDD0662  [4]
 LDCM0346  AC80 HCT 116 C322(0.74); C330(0.74)  LDD0663  [4]
 LDCM0347  AC81 HCT 116 C322(0.80); C330(0.80)  LDD0664  [4]
 LDCM0348  AC82 HCT 116 C322(0.50); C330(0.50)  LDD0665  [4]
 LDCM0365  AC98 HCT 116 C129(1.08)  LDD0682  [4]
 LDCM0366  AC99 HCT 116 C129(1.05)  LDD0683  [4]
 LDCM0248  AKOS034007472 HCT 116 C129(1.13); C322(0.69); C330(0.69)  LDD0565  [4]
 LDCM0356  AKOS034007680 HCT 116 C322(0.61); C330(0.61); C129(1.35)  LDD0673  [4]
 LDCM0275  AKOS034007705 HCT 116 C322(0.45); C330(0.45); C129(1.43)  LDD0592  [4]
 LDCM0632  CL-Sc Hep-G2 C371(0.52)  LDD2227  [3]
 LDCM0367  CL1 PaTu 8988t C371(0.80)  LDD1246  [4]
 LDCM0368  CL10 PaTu 8988t C371(1.06)  LDD1247  [4]
 LDCM0369  CL100 PaTu 8988t C371(0.50)  LDD1248  [4]
 LDCM0370  CL101 HCT 116 C322(0.66); C330(0.66); C129(1.14)  LDD0687  [4]
 LDCM0371  CL102 HCT 116 C322(0.75); C330(0.75); C129(0.95)  LDD0688  [4]
 LDCM0372  CL103 HCT 116 C322(1.09); C330(1.09); C129(1.14)  LDD0689  [4]
 LDCM0373  CL104 HCT 116 C322(1.17); C330(1.17); C129(1.25)  LDD0690  [4]
 LDCM0374  CL105 HEK-293T C129(1.02); C371(1.13); C304(1.04)  LDD1578  [11]
 LDCM0375  CL106 HEK-293T C129(0.96); C304(0.94)  LDD1579  [11]
 LDCM0376  CL107 HEK-293T C129(0.97); C304(1.60)  LDD1580  [11]
 LDCM0377  CL108 HEK-293T C129(0.85); C371(0.90); C304(1.15)  LDD1581  [11]
 LDCM0378  CL109 HEK-293T C129(0.97); C371(1.03); C304(1.11)  LDD1582  [11]
 LDCM0379  CL11 PaTu 8988t C371(0.99)  LDD1258  [4]
 LDCM0380  CL110 HEK-293T C129(0.93); C304(0.99)  LDD1584  [11]
 LDCM0381  CL111 HEK-293T C129(0.92); C304(1.25)  LDD1585  [11]
 LDCM0382  CL112 PaTu 8988t C371(0.96)  LDD1261  [4]
 LDCM0383  CL113 PaTu 8988t C371(0.78)  LDD1262  [4]
 LDCM0384  CL114 PaTu 8988t C371(0.88)  LDD1263  [4]
 LDCM0385  CL115 PaTu 8988t C371(0.95)  LDD1264  [4]
 LDCM0386  CL116 PaTu 8988t C371(1.31)  LDD1265  [4]
 LDCM0387  CL117 HCT 116 C129(0.68)  LDD0704  [4]
 LDCM0388  CL118 HCT 116 C129(1.06)  LDD0705  [4]
 LDCM0389  CL119 HCT 116 C129(1.00)  LDD0706  [4]
 LDCM0390  CL12 PaTu 8988t C371(1.09)  LDD1269  [4]
 LDCM0391  CL120 HCT 116 C129(1.09)  LDD0708  [4]
 LDCM0392  CL121 HEK-293T C129(1.06); C371(1.14); C304(1.04)  LDD1596  [11]
 LDCM0393  CL122 HEK-293T C129(0.96); C304(1.09)  LDD1597  [11]
 LDCM0394  CL123 HEK-293T C129(1.00); C304(1.35)  LDD1598  [11]
 LDCM0395  CL124 HEK-293T C129(1.02); C371(0.99); C304(0.94)  LDD1599  [11]
 LDCM0396  CL125 HEK-293T C129(1.08); C371(1.00); C304(1.25)  LDD1600  [11]
 LDCM0397  CL126 HEK-293T C129(0.94); C304(1.03)  LDD1601  [11]
 LDCM0398  CL127 HEK-293T C129(1.00); C304(1.38)  LDD1602  [11]
 LDCM0399  CL128 HEK-293T C129(0.86); C371(0.86); C304(1.02)  LDD1603  [11]
 LDCM0400  CL13 PaTu 8988t C371(1.17)  LDD1279  [4]
 LDCM0401  CL14 PaTu 8988t C371(1.09)  LDD1280  [4]
 LDCM0402  CL15 PaTu 8988t C371(1.42)  LDD1281  [4]
 LDCM0403  CL16 HCT 116 C322(0.72); C330(0.72)  LDD0720  [4]
 LDCM0404  CL17 HCT 116 C322(0.63); C330(0.63); C129(0.96)  LDD0721  [4]
 LDCM0405  CL18 HCT 116 C322(0.56); C330(0.56); C129(1.39)  LDD0722  [4]
 LDCM0406  CL19 HCT 116 C322(0.66); C330(0.66); C129(1.07)  LDD0723  [4]
 LDCM0407  CL2 PaTu 8988t C371(0.97)  LDD1286  [4]
 LDCM0408  CL20 HCT 116 C322(0.81); C330(0.81); C129(1.14)  LDD0725  [4]
 LDCM0409  CL21 HCT 116 C322(0.72); C330(0.72); C129(0.93)  LDD0726  [4]
 LDCM0410  CL22 HCT 116 C322(0.58); C330(0.58); C129(1.30)  LDD0727  [4]
 LDCM0411  CL23 HCT 116 C322(0.93); C330(0.93); C129(1.03)  LDD0728  [4]
 LDCM0412  CL24 HCT 116 C322(0.77); C330(0.77); C129(1.06)  LDD0729  [4]
 LDCM0413  CL25 HCT 116 C129(1.26); C322(0.76); C330(0.76)  LDD0730  [4]
 LDCM0414  CL26 HCT 116 C129(1.14); C322(0.77); C330(0.77)  LDD0731  [4]
 LDCM0415  CL27 HCT 116 C129(1.26); C322(0.83); C330(0.83)  LDD0732  [4]
 LDCM0416  CL28 HCT 116 C129(1.43); C322(0.86); C330(0.86)  LDD0733  [4]
 LDCM0417  CL29 HCT 116 C129(1.41); C322(0.70); C330(0.70)  LDD0734  [4]
 LDCM0418  CL3 PaTu 8988t C371(1.19)  LDD1297  [4]
 LDCM0419  CL30 HCT 116 C129(1.11); C322(0.90); C330(0.90)  LDD0736  [4]
 LDCM0420  CL31 HCT 116 C129(0.94)  LDD0737  [4]
 LDCM0421  CL32 HCT 116 C129(1.24)  LDD0738  [4]
 LDCM0422  CL33 HCT 116 C129(1.02)  LDD0739  [4]
 LDCM0423  CL34 HCT 116 C129(0.67)  LDD0740  [4]
 LDCM0424  CL35 HCT 116 C129(0.88)  LDD0741  [4]
 LDCM0425  CL36 HCT 116 C129(0.94)  LDD0742  [4]
 LDCM0426  CL37 HCT 116 C129(0.89)  LDD0743  [4]
 LDCM0428  CL39 HCT 116 C129(0.93)  LDD0745  [4]
 LDCM0429  CL4 PaTu 8988t C371(1.08)  LDD1308  [4]
 LDCM0430  CL40 HCT 116 C129(0.92)  LDD0747  [4]
 LDCM0431  CL41 HCT 116 C129(0.97)  LDD0748  [4]
 LDCM0432  CL42 HCT 116 C129(0.90)  LDD0749  [4]
 LDCM0433  CL43 HCT 116 C129(1.05)  LDD0750  [4]
 LDCM0434  CL44 HCT 116 C129(0.78)  LDD0751  [4]
 LDCM0435  CL45 HCT 116 C129(1.04)  LDD0752  [4]
 LDCM0436  CL46 HEK-293T C129(0.97); C371(1.01)  LDD1640  [11]
 LDCM0437  CL47 HEK-293T C129(1.32); C371(0.92); C304(0.81)  LDD1641  [11]
 LDCM0438  CL48 HEK-293T C129(0.39); C371(0.83)  LDD1642  [11]
 LDCM0439  CL49 HEK-293T C129(1.02); C371(1.08); C304(1.01)  LDD1643  [11]
 LDCM0440  CL5 PaTu 8988t C371(1.16)  LDD1319  [4]
 LDCM0441  CL50 HEK-293T C129(1.00); C304(1.03)  LDD1645  [11]
 LDCM0443  CL52 HEK-293T C129(0.98); C371(1.06); C304(1.15)  LDD1646  [11]
 LDCM0444  CL53 HEK-293T C129(0.94); C371(0.95); C304(1.34)  LDD1647  [11]
 LDCM0445  CL54 HEK-293T C129(1.01); C371(1.00); C304(1.11)  LDD1648  [11]
 LDCM0446  CL55 HEK-293T C129(1.20); C371(1.11); C304(0.92)  LDD1649  [11]
 LDCM0447  CL56 HEK-293T C129(1.33); C371(1.02); C304(0.69)  LDD1650  [11]
 LDCM0448  CL57 HEK-293T C129(1.39); C371(0.93); C304(0.96)  LDD1651  [11]
 LDCM0449  CL58 HEK-293T C129(1.00); C371(1.04)  LDD1652  [11]
 LDCM0450  CL59 HEK-293T C129(1.29); C371(1.02); C304(0.97)  LDD1653  [11]
 LDCM0451  CL6 PaTu 8988t C371(1.12)  LDD1330  [4]
 LDCM0452  CL60 HEK-293T C129(0.51); C371(0.83)  LDD1655  [11]
 LDCM0453  CL61 PaTu 8988t C371(0.97)  LDD1332  [4]
 LDCM0454  CL62 PaTu 8988t C371(1.07)  LDD1333  [4]
 LDCM0455  CL63 PaTu 8988t C371(1.25)  LDD1334  [4]
 LDCM0456  CL64 PaTu 8988t C371(1.08)  LDD1335  [4]
 LDCM0457  CL65 PaTu 8988t C371(0.97)  LDD1336  [4]
 LDCM0458  CL66 PaTu 8988t C371(0.81)  LDD1337  [4]
 LDCM0459  CL67 PaTu 8988t C371(1.20)  LDD1338  [4]
 LDCM0460  CL68 PaTu 8988t C371(0.95)  LDD1339  [4]
 LDCM0461  CL69 PaTu 8988t C371(1.40)  LDD1340  [4]
 LDCM0462  CL7 PaTu 8988t C371(0.90)  LDD1341  [4]
 LDCM0463  CL70 PaTu 8988t C371(1.06)  LDD1342  [4]
 LDCM0464  CL71 PaTu 8988t C371(0.86)  LDD1343  [4]
 LDCM0465  CL72 PaTu 8988t C371(0.87)  LDD1344  [4]
 LDCM0466  CL73 PaTu 8988t C371(1.37)  LDD1345  [4]
 LDCM0467  CL74 PaTu 8988t C371(0.98)  LDD1346  [4]
 LDCM0469  CL76 HEK-293T C129(0.89); C371(1.24); C304(1.14)  LDD1672  [11]
 LDCM0470  CL77 HEK-293T C129(0.95); C371(1.08); C304(0.99)  LDD1673  [11]
 LDCM0471  CL78 HEK-293T C129(1.13); C371(1.16); C304(1.24)  LDD1674  [11]
 LDCM0472  CL79 HEK-293T C129(1.24); C371(1.35); C304(0.96)  LDD1675  [11]
 LDCM0473  CL8 PaTu 8988t C371(1.38)  LDD1352  [4]
 LDCM0474  CL80 HEK-293T C129(1.23); C371(0.93); C304(1.37)  LDD1677  [11]
 LDCM0475  CL81 HEK-293T C129(1.44); C371(1.10); C304(0.94)  LDD1678  [11]
 LDCM0476  CL82 HEK-293T C129(0.98); C371(0.81)  LDD1679  [11]
 LDCM0477  CL83 HEK-293T C129(1.30); C371(1.02); C304(0.73)  LDD1680  [11]
 LDCM0478  CL84 HEK-293T C129(0.56); C371(0.81)  LDD1681  [11]
 LDCM0479  CL85 HEK-293T C129(1.12); C371(1.06); C304(1.05)  LDD1682  [11]
 LDCM0480  CL86 HEK-293T C129(1.09); C304(1.20)  LDD1683  [11]
 LDCM0481  CL87 HEK-293T C129(1.11); C304(1.29)  LDD1684  [11]
 LDCM0482  CL88 HEK-293T C129(1.01); C371(1.09); C304(1.03)  LDD1685  [11]
 LDCM0483  CL89 HEK-293T C129(1.07); C371(1.03); C304(1.42)  LDD1686  [11]
 LDCM0484  CL9 PaTu 8988t C371(0.98)  LDD1363  [4]
 LDCM0485  CL90 HEK-293T C129(1.01); C371(0.97); C304(0.91)  LDD1688  [11]
 LDCM0486  CL91 PaTu 8988t C371(0.40)  LDD1365  [4]
 LDCM0487  CL92 PaTu 8988t C371(0.48)  LDD1366  [4]
 LDCM0488  CL93 PaTu 8988t C371(0.43)  LDD1367  [4]
 LDCM0489  CL94 PaTu 8988t C371(0.48)  LDD1368  [4]
 LDCM0490  CL95 PaTu 8988t C371(0.57)  LDD1369  [4]
 LDCM0491  CL96 PaTu 8988t C371(0.42)  LDD1370  [4]
 LDCM0492  CL97 PaTu 8988t C371(0.43)  LDD1371  [4]
 LDCM0493  CL98 PaTu 8988t C371(0.52)  LDD1372  [4]
 LDCM0494  CL99 PaTu 8988t C371(0.40)  LDD1373  [4]
 LDCM0495  E2913 HEK-293T C129(1.11); C304(1.28)  LDD1698  [11]
 LDCM0468  Fragment33 PaTu 8988t C371(1.02)  LDD1347  [4]
 LDCM0427  Fragment51 HCT 116 C129(1.00)  LDD0744  [4]
 LDCM0022  KB02 HEK-293T C129(1.00); C304(0.70)  LDD1492  [11]
 LDCM0023  KB03 HEK-293T C129(1.00); C304(1.12)  LDD1497  [11]
 LDCM0024  KB05 MIA PaCa-2 C304(1.35)  LDD3330  [12]
 LDCM0099  Phenelzine HEK-293T 8.00  LDD0390  [1]

References

1 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
9 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
12 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840