Details of the Target

General Information of Target

Target ID LDTP05069
Target Name Actin, alpha cardiac muscle 1 (ACTC1)
Gene Name ACTC1
Gene ID 70
Synonyms
ACTC; Actin, alpha cardiac muscle 1; EC 3.6.4.-; Alpha-cardiac actin) [Cleaved into: Actin, alpha cardiac muscle 1, intermediate form]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
QSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREK
MTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRL
DLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV
LSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIS
KQEYDEAGPSIVHRKCF
Target Bioclass
Enzyme
Family
Actin family
Subcellular location
Cytoplasm, cytoskeleton
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Uniprot ID
P68032
Ensemble ID
ENST00000290378.6
HGNC ID
HGNC:143

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y93(13.18); Y71(8.60)  LDD0257  [1]
TH214
 Probe Info  		Y93(13.85); Y71(11.61)  LDD0258  [1]
TH216
 Probe Info  		Y93(14.07); Y71(6.42)  LDD0259  [1]
ONAyne
 Probe Info  		K293(1.30); K70(4.29); K86(3.76)  LDD0274  [2]
STPyne
 Probe Info  		K70(8.89)  LDD0277  [2]
HHS-482
 Probe Info  		Y71(0.85)  LDD0285  [3]
HHS-475
 Probe Info  		Y93(0.53); Y242(0.75); Y55(0.77); Y71(0.79)  LDD0264  [4]
HHS-465
 Probe Info  		Y190(10.00); Y242(10.00); Y55(10.00); Y71(9.20)  LDD2237  [5]
5E-2FA
 Probe Info  		N.A.  LDD2235  [6]
m-APA
 Probe Info  		H89(0.00); H75(0.00)  LDD2231  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [7]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [8]
2PCA
 Probe Info  		K20(0.00); R314(0.00); K240(0.00); R41(0.00)  LDD0034  [9]
ATP probe
 Probe Info  		K52(0.00); K63(0.00); K330(0.00); K86(0.00)  LDD0035  [10]
AZ-9
 Probe Info  		D27(0.00); E243(0.00)  LDD0395  [11]
Ox-W18
 Probe Info  		W81(0.00); W88(0.00)  LDD2175  [12]
1c-yne
 Probe Info  		K293(0.00); K70(0.00); K86(0.00); K215(0.00)  LDD0228  [13]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		5.06  LDD0472  [14]
STS-1
 Probe Info  		3.64  LDD0136  [15]
STS-2
 Probe Info  		1.38  LDD0138  [15]
VE-P
 Probe Info  		N.A.  LDD0396  [16]
Photocelecoxib
 Probe Info  		K86(0.00); L107(0.00)  LDD0153  [17]
Photoindomethacin
 Probe Info  		N.A.  LDD0155  [17]
DA-2
 Probe Info  		N.A.  LDD0070  [18]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0630  CCW28-3 231MFP C219(1.69)  LDD2214  [19]
 LDCM0116  HHS-0101 DM93 Y93(0.53); Y242(0.75); Y55(0.77); Y71(0.79)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y93(0.38); Y339(0.60); Y242(0.62); Y55(0.67)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y55(0.71); Y93(0.73); Y71(0.85); Y190(1.11)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y339(0.63); Y93(0.72); Y242(0.73); Y55(0.75)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y93(0.38); Y242(0.52); Y55(0.71); Y190(1.00)  LDD0268  [4]
 LDCM0123  JWB131 DM93 Y71(0.85)  LDD0285  [3]
 LDCM0124  JWB142 DM93 Y71(0.49)  LDD0286  [3]
 LDCM0125  JWB146 DM93 Y71(1.00)  LDD0287  [3]
 LDCM0126  JWB150 DM93 Y71(2.84)  LDD0288  [3]
 LDCM0127  JWB152 DM93 Y71(1.91)  LDD0289  [3]
 LDCM0128  JWB198 DM93 Y71(1.03)  LDD0290  [3]
 LDCM0129  JWB202 DM93 Y71(0.45)  LDD0291  [3]
 LDCM0130  JWB211 DM93 Y71(0.80)  LDD0292  [3]

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 Unveiling the Multifaceted Capabilities of Endophytic Aspergillus flavus Isolated from Annona squamosa Fruit Peels against Staphylococcus Isolates and HCoV 229E-In Vitro and In Silico Investigations. Pharmaceuticals (Basel). 2024 May 19;17(5):656. doi: 10.3390/ph17050656.
Mass spectrometry data entry: PXD013019
10 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
11 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
12 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
13 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
14 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
15 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
16 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
17 Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J Am Chem Soc. 2018 Mar 28;140(12):4259-4268. doi: 10.1021/jacs.7b11639. Epub 2018 Mar 15.
Mass spectrometry data entry: PXD007094
18 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
19 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.