Details of the Target

General Information of Target

Target ID LDTP03816
Target Name Oxidized purine nucleoside triphosphate hydrolase (NUDT1)
Gene Name NUDT1
Gene ID 4521
Synonyms
MTH1; Oxidized purine nucleoside triphosphate hydrolase; EC 3.6.1.56; 2-hydroxy-dATP diphosphatase; 7,8-dihydro-8-oxoguanine triphosphatase; 8-oxo-dGTPase; Methylated purine nucleoside triphosphate hydrolase; EC 3.6.1.-; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGASRLYTLVLVLQPQRVLLGMKKRGFGAGRWNGFGGKVQEGETIEDGARRELQEESGLT
VDALHKVGQIVFEFVGEPELMDVHVFCTDSIQGTPVESDEMRPCWFQLDQIPFKDMWPDD
SYWFPLLLQKKKFHGYFKFQGQDTILDYTLREVDTV
Target Bioclass
Enzyme
Family
Nudix hydrolase family
Subcellular location
Cytoplasm, cytosol; Mitochondrion matrix
Function
Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A . Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA. Through this antimutagenic activity protects cells from oxidative stress.
Uniprot ID
P36639
Ensemble ID
ENST00000339737.6
HGNC ID
HGNC:8048
ChEMBL ID
CHEMBL3708265

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Alkylaryl probe 3
 Probe Info  		20.00  LDD0381  [1]
AZ-9
 Probe Info  		4.46  LDD0393  [2]
FBPP2
 Probe Info  		25.67  LDD0318  [3]
TH211
 Probe Info  		Y48(20.00)  LDD0257  [4]
TH216
 Probe Info  		Y48(20.00)  LDD0259  [4]
STPyne
 Probe Info  		K173(7.32)  LDD0277  [5]
HHS-475
 Probe Info  		Y48(1.04)  LDD0264  [6]
DBIA
 Probe Info  		C104(0.72)  LDD1575  [7]
ATP probe
 Probe Info  		N.A.  LDD0199  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [9]
ATP probe
 Probe Info  		K23(0.00); K38(0.00)  LDD0035  [10]
PAL-AfBPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C041
 Probe Info  		5.94  LDD1741  [11]
C087
 Probe Info  		7.16  LDD1779  [11]
C193
 Probe Info  		5.21  LDD1869  [11]
C269
 Probe Info  		6.50  LDD1939  [11]
C390
 Probe Info  		43.41  LDD2049  [11]
C391
 Probe Info  		24.59  LDD2050  [11]
Kambe_31
 Probe Info  		9.36  LDD0128  [12]
Kambe_8
 Probe Info  		2.13  LDD0131  [12]
OEA-DA
 Probe Info  		20.00  LDD0046  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0371  CL102 HEK-293T C104(0.72)  LDD1575  [7]
 LDCM0375  CL106 HEK-293T C104(1.09)  LDD1579  [7]
 LDCM0380  CL110 HEK-293T C104(0.71)  LDD1584  [7]
 LDCM0384  CL114 HEK-293T C104(0.63)  LDD1588  [7]
 LDCM0388  CL118 HEK-293T C104(0.86)  LDD1592  [7]
 LDCM0393  CL122 HEK-293T C104(0.91)  LDD1597  [7]
 LDCM0397  CL126 HEK-293T C104(0.79)  LDD1601  [7]
 LDCM0401  CL14 HEK-293T C104(1.04)  LDD1605  [7]
 LDCM0407  CL2 HEK-293T C104(1.19)  LDD1611  [7]
 LDCM0414  CL26 HEK-293T C104(1.05)  LDD1618  [7]
 LDCM0441  CL50 HEK-293T C104(0.81)  LDD1645  [7]
 LDCM0454  CL62 HEK-293T C104(0.78)  LDD1657  [7]
 LDCM0467  CL74 HEK-293T C104(0.69)  LDD1670  [7]
 LDCM0480  CL86 HEK-293T C104(0.56)  LDD1683  [7]
 LDCM0493  CL98 HEK-293T C104(0.91)  LDD1696  [7]
 LDCM0427  Fragment51 HEK-293T C104(1.11)  LDD1631  [7]
 LDCM0116  HHS-0101 DM93 Y48(1.04)  LDD0264  [6]
 LDCM0117  HHS-0201 DM93 Y48(0.71)  LDD0265  [6]
 LDCM0118  HHS-0301 DM93 Y48(0.74)  LDD0266  [6]
 LDCM0119  HHS-0401 DM93 Y48(0.78)  LDD0267  [6]
 LDCM0120  HHS-0701 DM93 Y48(1.44)  LDD0268  [6]
 LDCM0073  Kambe_cp3 PC-3 9.36  LDD0128  [12]
 LDCM0075  kambe_cp62 PC-3 2.13  LDD0131  [12]
 LDCM0079  kambe_cp64 PC-3 2.30  LDD0135  [12]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
tRNA wybutosine-synthesizing protein 2 homolog (TRMT12) TRM5/TYW2 family Q53H54
Heme oxygenase 2 (HMOX2) Heme oxygenase family P30519
Caspase-6 (CASP6) Peptidase C14A family P55212
Aminoacylase-1 (ACY1) Peptidase M20A family Q03154
GTP-binding nuclear protein Ran (RAN) Ran family P62826
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Huntingtin-interacting protein 1 (HIP1) SLA2 family O00291
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Heat shock protein beta-1 (HSPB1) Small heat shock protein (HSP20) family P04792
RING finger protein 11 (RNF11) . Q9Y3C5

References

1 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
11 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
12 Mapping the protein interaction landscape for fully functionalized small-molecule probes in human cells. J Am Chem Soc. 2014 Jul 30;136(30):10777-82. doi: 10.1021/ja505517t. Epub 2014 Jul 21.
13 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570