Details of the Target

General Information of Target

Target ID LDTP09556
Target Name E3 ubiquitin-protein ligase RNF139 (RNF139)
Gene Name RNF139
Gene ID 11236
Synonyms
TRC8; E3 ubiquitin-protein ligase RNF139; EC 2.3.2.27; RING finger protein 139; RING-type E3 ubiquitin transferase RNF139; Translocation in renal carcinoma on chromosome 8 protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAVGPPQQQVRMAHQQVWAALEVALRVPCLYIIDAIFNSYPDSSQSRFCIVLQIFLRLF
GVFASSIVLILSQRSLFKFYTYSSAFLLAATSVLVNYYASLHIDFYGAYNTSAFGIELLP
RKGPSLWMALIVLQLTFGIGYVTLLQIHSIYSQLIILDLLVPVIGLITELPLHIRETLLF
TSSLILTLNTVFVLAVKLKWFYYSTRYVYLLVRHMYRIYGLQLLMEDTWKRIRFPDILRV
FWLTRVTAQATVLMYILRMANETDSFFISWDDFWDLICNLIISGCDSTLTVLGMSAVISS
VAHYLGLGILAFIGSTEEDDRRLGFVAPVLFFILALQTGLSGLRPEERLIRLSRNMCLLL
TAVLHFIHGMTDPVLMSLSASHVSSFRRHFPVLFVSACLFILPVLLSYVLWHHYALNTWL
FAVTAFCVELCLKVIVSLTVYTLFMIDGYYNVLWEKLDDYVYYVRSTGSIIEFIFGVVMF
GNGAYTMMFESGSKIRAFMMCLHAYFNIYLQAKNGWKTFMNRRTAVKKINSLPEIKGSRL
QEINDVCAICYHEFTTSARITPCNHYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSN
VSNNNGFIPPNETPEEAVREAAAESDRELNEDDSTDCDDDVQRERNGVIQHTGAAAEEFN
DDTD
Target Bioclass
Enzyme
Subcellular location
Endoplasmic reticulum membrane
Function
E3-ubiquitin ligase; acts as a negative regulator of cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP-SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Involved in the sterol-accelerated degradation of HMGCR. This is achieved through binding of RNF139 to INSIG1 and/or INSIG2 at the ER membrane. In addition, interaction of RNF139 with AUP1 facilitates interaction of RNF139 with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase AMFR, leading to ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination.
Uniprot ID
Q8WU17
Ensemble ID
ENST00000303545.4
HGNC ID
HGNC:17023

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Johansson_61
 Probe Info  		_(20.00)  LDD1487  [1]
DBIA
 Probe Info  		C582(1.45); C585(1.45)  LDD0637  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [3]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C178
 Probe Info  		17.15  LDD1857  [4]
C218
 Probe Info  		21.86  LDD1892  [4]
C219
 Probe Info  		6.68  LDD1893  [4]
C220
 Probe Info  		29.04  LDD1894  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C30(0.78)  LDD1510  [5]
 LDCM0259  AC14 HEK-293T C30(0.81)  LDD1512  [5]
 LDCM0280  AC20 HEK-293T C30(0.75)  LDD1519  [5]
 LDCM0282  AC22 HEK-293T C30(0.63)  LDD1521  [5]
 LDCM0288  AC28 HEK-293T C30(0.85)  LDD1527  [5]
 LDCM0291  AC30 HEK-293T C30(1.02)  LDD1530  [5]
 LDCM0297  AC36 HEK-293T C30(0.95)  LDD1536  [5]
 LDCM0299  AC38 HEK-293T C30(0.91)  LDD1538  [5]
 LDCM0301  AC4 HEK-293T C30(0.94)  LDD1540  [5]
 LDCM0306  AC44 HEK-293T C30(0.96)  LDD1545  [5]
 LDCM0308  AC46 HEK-293T C30(0.75)  LDD1547  [5]
 LDCM0315  AC52 HEK-293T C30(0.82)  LDD1554  [5]
 LDCM0317  AC54 HEK-293T C30(0.89)  LDD1556  [5]
 LDCM0320  AC57 HCT 116 C582(1.45); C585(1.45)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C582(0.96); C585(0.96)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C582(1.17); C585(1.17)  LDD0639  [2]
 LDCM0323  AC6 HEK-293T C30(0.82)  LDD1562  [5]
 LDCM0324  AC60 HCT 116 C582(0.81); C585(0.81)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C582(0.88); C585(0.88)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C582(0.88); C585(0.88)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C582(0.73); C585(0.73)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C582(0.86); C585(0.86)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C582(0.87); C585(0.87)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C582(0.96); C585(0.96)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C582(0.58); C585(0.58)  LDD0648  [2]
 LDCM0332  AC68 HCT 116 C582(0.92); C585(0.92)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C582(0.86); C585(0.86)  LDD0650  [2]
 LDCM0335  AC70 HCT 116 C582(0.65); C585(0.65)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C582(0.87); C585(0.87)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C582(0.80); C585(0.80)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C582(0.63); C585(0.63)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C582(0.66); C585(0.66)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C582(0.59); C585(0.59)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C582(0.90); C585(0.90)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C582(0.92); C585(0.92)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C582(0.82); C585(0.82)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C582(0.91); C585(0.91)  LDD0661  [2]
 LDCM0346  AC80 HCT 116 C582(0.82); C585(0.82)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C582(0.87); C585(0.87)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C582(0.41); C585(0.41)  LDD0665  [2]
 LDCM0368  CL10 HEK-293T C30(1.14)  LDD1572  [5]
 LDCM0396  CL125 HCT 116 C582(2.16); C585(2.16)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C582(1.45); C585(1.45)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C582(1.16); C585(1.16)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C582(0.80); C585(0.80)  LDD0716  [2]
 LDCM0403  CL16 HCT 116 C582(1.18); C585(1.18)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C582(0.85); C585(0.85)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C582(0.94); C585(0.94)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C582(0.96); C585(0.96)  LDD0723  [2]
 LDCM0408  CL20 HCT 116 C582(1.30); C585(1.30)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C582(1.02); C585(1.02)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C582(0.98); C585(0.98)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C582(1.19); C585(1.19)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C582(0.91); C585(0.91)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C582(1.16); C585(1.16)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C582(1.04); C585(1.04)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C582(1.03); C585(1.03)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C582(1.01); C585(1.01)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C582(0.84); C585(0.84)  LDD0734  [2]
 LDCM0419  CL30 HCT 116 C582(1.18); C585(1.18)  LDD0736  [2]
 LDCM0420  CL31 HCT 116 C582(0.60); C585(0.60)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C582(0.70); C585(0.70)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C582(0.89); C585(0.89)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C582(0.59); C585(0.59)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C582(0.69); C585(0.69)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C582(0.51); C585(0.51)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C582(0.98); C585(0.98)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C582(0.66); C585(0.66)  LDD0745  [2]
 LDCM0430  CL40 HCT 116 C582(0.66); C585(0.66)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C582(0.73); C585(0.73)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C582(0.75); C585(0.75)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C582(0.63); C585(0.63)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C582(0.50); C585(0.50)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C582(0.62); C585(0.62)  LDD0752  [2]
 LDCM0436  CL46 HEK-293T C30(1.00)  LDD1640  [5]
 LDCM0447  CL56 HEK-293T C30(0.90)  LDD1650  [5]
 LDCM0449  CL58 HEK-293T C30(1.01)  LDD1652  [5]
 LDCM0460  CL68 HEK-293T C30(0.98)  LDD1663  [5]
 LDCM0463  CL70 HEK-293T C30(0.77)  LDD1666  [5]
 LDCM0473  CL8 HEK-293T C30(0.89)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C30(0.80)  LDD1677  [5]
 LDCM0476  CL82 HEK-293T C30(0.86)  LDD1679  [5]
 LDCM0487  CL92 HEK-293T C30(1.02)  LDD1690  [5]
 LDCM0489  CL94 HEK-293T C30(1.17)  LDD1692  [5]
 LDCM0427  Fragment51 HCT 116 C582(0.95); C585(0.95)  LDD0744  [2]
 LDCM0615  Fragment63-R Jurkat _(20.00)  LDD1487  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Minor histocompatibility antigen H13 (HM13) Peptidase A22B family Q8TCT9
von Hippel-Lindau disease tumor suppressor (VHL) VHL family P40337
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253

References

1 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402