Details of the Target

General Information of Target

Target ID LDTP08885
Target Name Actin filament-associated protein 1 (AFAP1)
Gene Name AFAP1
Gene ID 60312
Synonyms
AFAP; Actin filament-associated protein 1; 110 kDa actin filament-associated protein; AFAP-110
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLP
APPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSS
SYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTK
LLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQW
LKVIKEAYSGCSGPVDSECPPPPSSPVHKAELEKKLSSERPSSDGEGVVENGITTCNGKE
QVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNS
RWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAV
LEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISA
NPYLGGTSNGYAHPSGTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPA
AVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIE
VNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGGVTLGLAIEPK
SGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCRGHVLRK
AKEWELKNGT
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton, stress fiber
Function
Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Uniprot ID
Q8N556
Ensemble ID
ENST00000358461.6
HGNC ID
HGNC:24017

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
CY4
 Probe Info  		100.00  LDD0244  [2]
ONAyne
 Probe Info  		K404(1.43)  LDD0274  [3]
STPyne
 Probe Info  		K28(8.44); K332(10.00); K333(10.00); K378(9.09)  LDD0277  [3]
DBIA
 Probe Info  		C797(3.28)  LDD3310  [4]
BTD
 Probe Info  		C713(0.61)  LDD1700  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [6]
NAIA_4
 Probe Info  		C174(0.00); C506(0.00); C684(0.00)  LDD2226  [7]
NAIA_5
 Probe Info  		C713(0.00); C684(0.00)  LDD2224  [7]
TFBX
 Probe Info  		N.A.  LDD0027  [8]
WYneO
 Probe Info  		N.A.  LDD0022  [9]
IPM
 Probe Info  		N.A.  LDD0005  [9]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [10]
Acrolein
 Probe Info  		C622(0.00); C174(0.00); C713(0.00)  LDD0217  [11]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [11]
W1
 Probe Info  		N.A.  LDD0236  [12]
AOyne
 Probe Info  		13.90  LDD0443  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C713(0.73)  LDD2117  [5]
 LDCM0025  4SU-RNA HEK-293T C713(2.17)  LDD0371  [14]
 LDCM0563  Abegg_cp(+)-11 HeLa C622(2.16)  LDD0314  [8]
 LDCM0215  AC10 HEK-293T C622(0.85)  LDD1508  [15]
 LDCM0226  AC11 HEK-293T C622(1.09)  LDD1509  [15]
 LDCM0237  AC12 HEK-293T C713(1.10)  LDD1510  [15]
 LDCM0259  AC14 HEK-293T C622(0.96)  LDD1512  [15]
 LDCM0277  AC18 HEK-293T C622(0.93)  LDD1516  [15]
 LDCM0278  AC19 HEK-293T C622(0.92)  LDD1517  [15]
 LDCM0279  AC2 HEK-293T C622(0.90)  LDD1518  [15]
 LDCM0280  AC20 HEK-293T C713(0.94)  LDD1519  [15]
 LDCM0282  AC22 HEK-293T C622(1.12)  LDD1521  [15]
 LDCM0284  AC24 HEK-293T C622(1.10)  LDD1523  [15]
 LDCM0286  AC26 HEK-293T C622(0.88)  LDD1525  [15]
 LDCM0287  AC27 HEK-293T C622(0.90)  LDD1526  [15]
 LDCM0288  AC28 HEK-293T C713(0.93)  LDD1527  [15]
 LDCM0290  AC3 HEK-293T C622(1.01)  LDD1529  [15]
 LDCM0291  AC30 HEK-293T C622(0.99)  LDD1530  [15]
 LDCM0293  AC32 HEK-293T C622(1.03)  LDD1532  [15]
 LDCM0295  AC34 HEK-293T C622(0.90)  LDD1534  [15]
 LDCM0296  AC35 HEK-293T C622(1.05)  LDD1535  [15]
 LDCM0297  AC36 HEK-293T C713(0.93)  LDD1536  [15]
 LDCM0299  AC38 HEK-293T C622(0.95)  LDD1538  [15]
 LDCM0301  AC4 HEK-293T C713(1.20)  LDD1540  [15]
 LDCM0302  AC40 HEK-293T C622(1.02)  LDD1541  [15]
 LDCM0304  AC42 HEK-293T C622(0.88)  LDD1543  [15]
 LDCM0305  AC43 HEK-293T C622(1.02)  LDD1544  [15]
 LDCM0306  AC44 HEK-293T C713(0.92)  LDD1545  [15]
 LDCM0308  AC46 HEK-293T C622(1.13)  LDD1547  [15]
 LDCM0310  AC48 HEK-293T C622(1.21)  LDD1549  [15]
 LDCM0313  AC50 HEK-293T C622(1.03)  LDD1552  [15]
 LDCM0314  AC51 HEK-293T C622(1.07)  LDD1553  [15]
 LDCM0315  AC52 HEK-293T C713(0.84)  LDD1554  [15]
 LDCM0317  AC54 HEK-293T C622(0.96)  LDD1556  [15]
 LDCM0319  AC56 HEK-293T C622(1.21)  LDD1558  [15]
 LDCM0321  AC58 HEK-293T C622(0.87)  LDD1560  [15]
 LDCM0322  AC59 HEK-293T C622(1.09)  LDD1561  [15]
 LDCM0323  AC6 HEK-293T C622(1.04)  LDD1562  [15]
 LDCM0324  AC60 HEK-293T C713(1.01)  LDD1563  [15]
 LDCM0326  AC62 HEK-293T C622(1.04)  LDD1565  [15]
 LDCM0328  AC64 HEK-293T C622(1.16)  LDD1567  [15]
 LDCM0345  AC8 HEK-293T C622(1.13)  LDD1569  [15]
 LDCM0275  AKOS034007705 HEK-293T C622(1.15)  LDD1514  [15]
 LDCM0156  Aniline NCI-H1299 11.52  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa C713(0.00); C622(0.00); C174(0.00)  LDD0222  [11]
 LDCM0368  CL10 HEK-293T C622(0.98)  LDD1572  [15]
 LDCM0369  CL100 HEK-293T C622(0.98); C713(1.11)  LDD1573  [15]
 LDCM0371  CL102 HEK-293T C622(0.80)  LDD1575  [15]
 LDCM0373  CL104 HEK-293T C622(0.99); C713(0.95)  LDD1577  [15]
 LDCM0375  CL106 HEK-293T C622(1.03)  LDD1579  [15]
 LDCM0377  CL108 HEK-293T C622(0.95); C713(1.03)  LDD1581  [15]
 LDCM0380  CL110 HEK-293T C622(0.83)  LDD1584  [15]
 LDCM0382  CL112 HEK-293T C622(1.03); C713(0.90)  LDD1586  [15]
 LDCM0384  CL114 HEK-293T C622(0.82)  LDD1588  [15]
 LDCM0386  CL116 HEK-293T C622(1.04); C713(1.06)  LDD1590  [15]
 LDCM0388  CL118 HEK-293T C622(0.87)  LDD1592  [15]
 LDCM0390  CL12 HEK-293T C622(1.07)  LDD1594  [15]
 LDCM0391  CL120 HEK-293T C622(1.10); C713(0.93)  LDD1595  [15]
 LDCM0393  CL122 HEK-293T C622(0.95)  LDD1597  [15]
 LDCM0395  CL124 HEK-293T C622(1.01); C713(0.90)  LDD1599  [15]
 LDCM0397  CL126 HEK-293T C622(0.88)  LDD1601  [15]
 LDCM0399  CL128 HEK-293T C622(1.10); C713(0.92)  LDD1603  [15]
 LDCM0401  CL14 HEK-293T C622(0.87)  LDD1605  [15]
 LDCM0403  CL16 HEK-293T C622(1.12); C713(0.92)  LDD1607  [15]
 LDCM0405  CL18 HEK-293T C622(0.86)  LDD1609  [15]
 LDCM0406  CL19 HEK-293T C622(0.95)  LDD1610  [15]
 LDCM0407  CL2 HEK-293T C622(0.93)  LDD1611  [15]
 LDCM0408  CL20 HEK-293T C713(0.92)  LDD1612  [15]
 LDCM0410  CL22 HEK-293T C622(1.16)  LDD1614  [15]
 LDCM0412  CL24 HEK-293T C622(0.97)  LDD1616  [15]
 LDCM0414  CL26 HEK-293T C622(1.00)  LDD1618  [15]
 LDCM0416  CL28 HEK-293T C622(0.96); C713(0.88)  LDD1620  [15]
 LDCM0419  CL30 HEK-293T C622(0.99)  LDD1623  [15]
 LDCM0420  CL31 HEK-293T C622(1.10)  LDD1624  [15]
 LDCM0421  CL32 HEK-293T C713(1.15)  LDD1625  [15]
 LDCM0423  CL34 HEK-293T C622(1.06)  LDD1627  [15]
 LDCM0425  CL36 HEK-293T C622(0.97)  LDD1629  [15]
 LDCM0429  CL4 HEK-293T C622(0.97); C713(1.02)  LDD1633  [15]
 LDCM0430  CL40 HEK-293T C622(0.99); C713(0.84)  LDD1634  [15]
 LDCM0432  CL42 HEK-293T C622(1.04)  LDD1636  [15]
 LDCM0433  CL43 HEK-293T C622(1.13)  LDD1637  [15]
 LDCM0434  CL44 HEK-293T C713(1.09)  LDD1638  [15]
 LDCM0436  CL46 HEK-293T C622(1.02)  LDD1640  [15]
 LDCM0438  CL48 HEK-293T C622(0.97)  LDD1642  [15]
 LDCM0441  CL50 HEK-293T C622(0.92)  LDD1645  [15]
 LDCM0443  CL52 HEK-293T C622(1.03); C713(1.16)  LDD1646  [15]
 LDCM0445  CL54 HEK-293T C622(0.95)  LDD1648  [15]
 LDCM0446  CL55 HEK-293T C622(1.17)  LDD1649  [15]
 LDCM0447  CL56 HEK-293T C713(1.09)  LDD1650  [15]
 LDCM0449  CL58 HEK-293T C622(0.97)  LDD1652  [15]
 LDCM0451  CL6 HEK-293T C622(0.89)  LDD1654  [15]
 LDCM0452  CL60 HEK-293T C622(1.14)  LDD1655  [15]
 LDCM0454  CL62 HEK-293T C622(0.83)  LDD1657  [15]
 LDCM0456  CL64 HEK-293T C622(1.18); C713(1.04)  LDD1659  [15]
 LDCM0458  CL66 HEK-293T C622(1.04)  LDD1661  [15]
 LDCM0459  CL67 HEK-293T C622(1.02)  LDD1662  [15]
 LDCM0460  CL68 HEK-293T C713(1.19)  LDD1663  [15]
 LDCM0462  CL7 HEK-293T C622(1.05)  LDD1665  [15]
 LDCM0463  CL70 HEK-293T C622(0.88)  LDD1666  [15]
 LDCM0465  CL72 HEK-293T C622(1.09)  LDD1668  [15]
 LDCM0467  CL74 HEK-293T C622(0.85)  LDD1670  [15]
 LDCM0469  CL76 HEK-293T C622(1.05); C713(0.95)  LDD1672  [15]
 LDCM0471  CL78 HEK-293T C622(1.08)  LDD1674  [15]
 LDCM0472  CL79 HEK-293T C622(0.96)  LDD1675  [15]
 LDCM0473  CL8 HEK-293T C713(0.76)  LDD1676  [15]
 LDCM0474  CL80 HEK-293T C713(0.90)  LDD1677  [15]
 LDCM0476  CL82 HEK-293T C622(0.97)  LDD1679  [15]
 LDCM0478  CL84 HEK-293T C622(1.07)  LDD1681  [15]
 LDCM0480  CL86 HEK-293T C622(0.84)  LDD1683  [15]
 LDCM0482  CL88 HEK-293T C622(0.99); C713(0.87)  LDD1685  [15]
 LDCM0485  CL90 HEK-293T C622(0.85)  LDD1688  [15]
 LDCM0486  CL91 HEK-293T C622(1.29)  LDD1689  [15]
 LDCM0487  CL92 HEK-293T C713(1.01)  LDD1690  [15]
 LDCM0489  CL94 HEK-293T C622(1.10)  LDD1692  [15]
 LDCM0491  CL96 HEK-293T C622(1.02)  LDD1694  [15]
 LDCM0493  CL98 HEK-293T C622(0.83)  LDD1696  [15]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C713(0.90)  LDD1702  [5]
 LDCM0576  Fragment14 MDA-MB-231 C713(1.13)  LDD1397  [16]
 LDCM0579  Fragment20 MDA-MB-231 C713(20.00)  LDD1402  [16]
 LDCM0580  Fragment21 MDA-MB-231 C713(1.13)  LDD1404  [16]
 LDCM0581  Fragment22 MDA-MB-231 C713(1.47)  LDD1406  [16]
 LDCM0578  Fragment27 MDA-MB-231 C713(0.94)  LDD1401  [16]
 LDCM0586  Fragment28 MDA-MB-231 C713(0.95)  LDD1415  [16]
 LDCM0589  Fragment31 MDA-MB-231 C713(1.31)  LDD1421  [16]
 LDCM0596  Fragment38 MDA-MB-231 C713(0.87)  LDD1433  [16]
 LDCM0598  Fragment40 MDA-MB-231 C713(0.72)  LDD1436  [16]
 LDCM0599  Fragment41 MDA-MB-231 C713(1.53)  LDD1438  [16]
 LDCM0605  Fragment47 MDA-MB-231 C713(1.03)  LDD1446  [16]
 LDCM0606  Fragment48 MDA-MB-231 C713(0.92)  LDD1447  [16]
 LDCM0427  Fragment51 MDA-MB-231 C713(1.14)  LDD1450  [16]
 LDCM0612  Fragment54 MDA-MB-231 C713(1.00)  LDD1456  [16]
 LDCM0614  Fragment56 MDA-MB-231 C713(0.92)  LDD1458  [16]
 LDCM0107  IAA HeLa N.A.  LDD0221  [11]
 LDCM0022  KB02 MDA-MB-231 C713(13.78)  LDD1374  [16]
 LDCM0023  KB03 22RV1 C797(2.16)  LDD2660  [4]
 LDCM0024  KB05 COLO792 C797(3.28)  LDD3310  [4]
 LDCM0109  NEM HeLa N.A.  LDD0224  [11]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C713(0.55)  LDD2107  [5]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C713(0.68)  LDD2125  [5]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C713(0.61)  LDD2127  [5]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C713(0.88)  LDD2136  [5]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C713(0.68)  LDD2137  [5]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C713(0.61)  LDD1700  [5]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
10 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
11 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
12 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
13 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
14 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
15 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
16 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.