Details of the Target

General Information of Target

Target ID LDTP05549
Target Name Protein phosphatase 3 catalytic subunit alpha (PPP3CA)
Gene Name PPP3CA
Gene ID 5530
Synonyms
CALNA; CNA; Protein phosphatase 3 catalytic subunit alpha; EC 3.1.3.16; CAM-PRP catalytic subunit; Calcineurin A alpha; Calmodulin-dependent calcineurin A subunit alpha isoform; CNA alpha; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESV
ALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYV
DRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMD
AFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFG
NEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP
SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEK
VTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESV
LTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRIN
ERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ
Target Type
Successful
Target Bioclass
Enzyme
Family
PPP phosphatase family, PP-2B subfamily
Subcellular location
Cytoplasm
Function
Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals. Dephosphorylates heat shock protein HSPB1. Dephosphorylates and activates transcription factor NFATC1. In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32. May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins. Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation. Required for postnatal development of the nephrogenic zone and superficial glomeruli in the kidneys, cell cycle homeostasis in the nephrogenic zone, and ultimately normal kidney function. Plays a role in intracellular AQP2 processing and localization to the apical membrane in the kidney, may thereby be required for efficient kidney filtration. Required for secretion of salivary enzymes amylase, peroxidase, lysozyme and sialic acid via formation of secretory vesicles in the submandibular glands. Required for calcineurin activity and homosynaptic depotentiation in the hippocampus. Required for normal differentiation and survival of keratinocytes and therefore required for epidermis superstructure formation. Positively regulates osteoblastic bone formation, via promotion of osteoblast differentiation. Positively regulates osteoclast differentiation, potentially via NFATC1 signaling. May play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling. Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB. Required for antigen-specific T-cell proliferation response. Dephosphorylates KLHL3, promoting the interaction between KLHL3 and WNK4 and subsequent degradation of WNK4. Negatively regulates SLC9A1 activity.
TTD ID
T25464
Uniprot ID
Q08209
DrugMap ID
TTA4LDE
Ensemble ID
ENST00000323055.10
HGNC ID
HGNC:9314
ChEMBL ID
CHEMBL4445

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.C336R .
EFO27 SNV: p.I403V .
HGC27 SNV: p.I520L DBIA    Probe Info 
IPC298 SNV: p.I81N .
LNCaP clone FGC SNV: p.G509V .
MCAS SNV: p.A494T .
MDST8 Deletion: p.N516_N519del .
MOLM13 SNV: p.S2Y .
NCIH23 SNV: p.R482W .
OVK18 SNV: p.C372Y .
SKMEL2 SNV: p.V436I .
TE4 SNV: p.D477A .
U87MG SNV: p.R217Ter .
UMUC3 SNV: p.E57Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y288(5.71)  LDD0257  [1]
STPyne
 Probe Info  		K10(10.00); K32(5.40); K466(7.59); K47(8.62)  LDD0277  [2]
DBIA
 Probe Info  		C88(1.95)  LDD3312  [3]
BTD
 Probe Info  		C153(0.79)  LDD2091  [4]
Acrolein
 Probe Info  		H250(0.00); H247(0.00)  LDD0221  [5]
IA-alkyne
 Probe Info  		C266(0.00); C256(0.00)  LDD0036  [6]
Lodoacetamide azide
 Probe Info  		C88(0.00); C228(0.00)  LDD0037  [6]
Ox-W18
 Probe Info  		N.A.  LDD2175  [7]
NAIA_5
 Probe Info  		C228(0.00); C129(0.00)  LDD2223  [8]
HHS-465
 Probe Info  		N.A.  LDD2240  [9]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C187
 Probe Info  		17.63  LDD1865  [10]
C191
 Probe Info  		12.38  LDD1868  [10]
C193
 Probe Info  		6.15  LDD1869  [10]
C284
 Probe Info  		22.32  LDD1954  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C153(1.14)  LDD2117  [4]
 LDCM0026  4SU-RNA+native RNA HEK-293T C88(4.62)  LDD0372  [11]
 LDCM0214  AC1 HEK-293T C88(0.82); C228(1.17); C256(1.23); C266(0.95)  LDD1507  [12]
 LDCM0215  AC10 HEK-293T C88(0.94); C372(0.96); C228(1.03); C256(0.99)  LDD1508  [12]
 LDCM0226  AC11 HEK-293T C88(1.08); C372(0.87); C228(1.19); C256(1.03)  LDD1509  [12]
 LDCM0237  AC12 HEK-293T C88(1.12); C228(1.03); C256(0.97); C266(1.01)  LDD1510  [12]
 LDCM0259  AC14 HEK-293T C88(1.03); C372(1.06); C228(1.21); C256(0.87)  LDD1512  [12]
 LDCM0270  AC15 HEK-293T C88(0.92); C228(1.07); C256(0.99); C266(1.06)  LDD1513  [12]
 LDCM0276  AC17 HEK-293T C88(0.91); C228(1.11); C256(1.03); C266(0.97)  LDD1515  [12]
 LDCM0277  AC18 HEK-293T C88(0.92); C372(0.82); C228(0.92); C256(1.00)  LDD1516  [12]
 LDCM0278  AC19 HEK-293T C88(1.13); C372(0.85); C228(1.12); C256(0.99)  LDD1517  [12]
 LDCM0279  AC2 HEK-293T C88(1.08); C372(1.56); C228(1.08); C256(1.07)  LDD1518  [12]
 LDCM0280  AC20 HEK-293T C88(0.82); C228(1.07); C256(1.02); C266(1.09)  LDD1519  [12]
 LDCM0281  AC21 HEK-293T C88(1.03); C372(1.09); C228(0.96); C256(1.11)  LDD1520  [12]
 LDCM0282  AC22 HEK-293T C88(0.99); C372(1.26); C228(0.98); C256(1.16)  LDD1521  [12]
 LDCM0283  AC23 HEK-293T C88(0.88); C228(1.11); C256(1.54); C266(1.01)  LDD1522  [12]
 LDCM0284  AC24 HEK-293T C88(0.99); C372(1.46); C228(0.86); C256(0.90)  LDD1523  [12]
 LDCM0285  AC25 HEK-293T C88(0.98); C228(1.03); C256(0.70); C266(1.04)  LDD1524  [12]
 LDCM0286  AC26 HEK-293T C88(1.02); C372(0.96); C228(0.96); C256(1.08)  LDD1525  [12]
 LDCM0287  AC27 HEK-293T C88(1.17); C372(0.82); C228(1.14); C256(0.98)  LDD1526  [12]
 LDCM0288  AC28 HEK-293T C88(1.08); C228(1.08); C256(0.85); C266(1.20)  LDD1527  [12]
 LDCM0289  AC29 HEK-293T C88(1.13); C372(1.24); C228(0.95); C256(1.16)  LDD1528  [12]
 LDCM0290  AC3 HEK-293T C88(1.17); C372(0.89); C228(1.10); C256(1.04)  LDD1529  [12]
 LDCM0291  AC30 HEK-293T C88(1.03); C372(1.19); C228(1.25); C256(1.55)  LDD1530  [12]
 LDCM0292  AC31 HEK-293T C88(0.96); C228(0.97); C256(1.07); C266(0.83)  LDD1531  [12]
 LDCM0293  AC32 HEK-293T C88(0.90); C372(1.57); C228(1.04); C256(0.76)  LDD1532  [12]
 LDCM0294  AC33 HEK-293T C88(0.91); C228(1.14); C256(0.85); C266(0.91)  LDD1533  [12]
 LDCM0295  AC34 HEK-293T C88(1.09); C372(0.86); C228(1.12); C256(0.93)  LDD1534  [12]
 LDCM0296  AC35 HEK-293T C88(1.13); C372(0.87); C228(1.04); C256(1.00)  LDD1535  [12]
 LDCM0297  AC36 HEK-293T C88(1.28); C228(1.02); C256(1.13); C266(1.12)  LDD1536  [12]
 LDCM0298  AC37 HEK-293T C88(1.01); C372(1.14); C228(1.13); C256(1.10)  LDD1537  [12]
 LDCM0299  AC38 HEK-293T C88(0.93); C372(1.18); C228(1.05); C256(0.89)  LDD1538  [12]
 LDCM0300  AC39 HEK-293T C88(0.87); C228(1.22); C256(1.07); C266(0.96)  LDD1539  [12]
 LDCM0301  AC4 HEK-293T C88(1.10); C228(1.06); C256(1.03); C266(1.03)  LDD1540  [12]
 LDCM0302  AC40 HEK-293T C88(1.04); C372(0.99); C228(0.87); C256(0.79)  LDD1541  [12]
 LDCM0303  AC41 HEK-293T C88(0.88); C228(1.07); C256(1.68); C266(1.07)  LDD1542  [12]
 LDCM0304  AC42 HEK-293T C88(0.93); C372(1.02); C228(0.98); C256(1.24)  LDD1543  [12]
 LDCM0305  AC43 HEK-293T C88(1.15); C372(0.87); C228(1.06); C256(1.02)  LDD1544  [12]
 LDCM0306  AC44 HEK-293T C88(1.03); C228(1.10); C256(0.90); C266(1.03)  LDD1545  [12]
 LDCM0307  AC45 HEK-293T C88(0.98); C372(1.42); C228(1.12); C256(1.07)  LDD1546  [12]
 LDCM0308  AC46 HEK-293T C88(1.03); C372(0.82); C228(1.11); C256(1.04)  LDD1547  [12]
 LDCM0309  AC47 HEK-293T C88(0.94); C228(1.17); C256(0.97); C266(0.90)  LDD1548  [12]
 LDCM0310  AC48 HEK-293T C88(1.02); C372(0.93); C228(0.96); C256(1.00)  LDD1549  [12]
 LDCM0311  AC49 HEK-293T C88(0.95); C228(1.05); C256(1.23); C266(1.10)  LDD1550  [12]
 LDCM0312  AC5 HEK-293T C88(1.05); C372(0.97); C228(1.18); C256(0.96)  LDD1551  [12]
 LDCM0313  AC50 HEK-293T C88(0.89); C372(0.93); C228(0.99); C256(1.01)  LDD1552  [12]
 LDCM0314  AC51 HEK-293T C88(1.13); C372(0.89); C228(1.19); C256(1.03)  LDD1553  [12]
 LDCM0315  AC52 HEK-293T C88(0.96); C228(0.90); C256(0.91); C266(0.97)  LDD1554  [12]
 LDCM0316  AC53 HEK-293T C88(0.98); C372(1.05); C228(1.00); C256(1.05)  LDD1555  [12]
 LDCM0317  AC54 HEK-293T C88(1.08); C372(1.09); C228(1.00); C256(1.02)  LDD1556  [12]
 LDCM0318  AC55 HEK-293T C88(0.98); C228(1.18); C256(1.32); C266(0.98)  LDD1557  [12]
 LDCM0319  AC56 HEK-293T C88(0.90); C372(0.98); C228(1.01); C256(1.01)  LDD1558  [12]
 LDCM0320  AC57 HEK-293T C88(0.94); C228(1.06); C256(1.46); C266(1.02)  LDD1559  [12]
 LDCM0321  AC58 HEK-293T C88(1.01); C372(0.73); C228(1.03); C256(1.13)  LDD1560  [12]
 LDCM0322  AC59 HEK-293T C88(1.09); C372(0.79); C228(1.10); C256(1.01)  LDD1561  [12]
 LDCM0323  AC6 HEK-293T C88(1.03); C372(1.07); C228(1.11); C256(1.27)  LDD1562  [12]
 LDCM0324  AC60 HEK-293T C88(1.09); C228(1.10); C256(1.08); C266(1.18)  LDD1563  [12]
 LDCM0325  AC61 HEK-293T C88(0.97); C372(1.16); C228(1.00); C256(1.01)  LDD1564  [12]
 LDCM0326  AC62 HEK-293T C88(1.01); C372(1.03); C228(1.00); C256(0.91)  LDD1565  [12]
 LDCM0327  AC63 HEK-293T C88(0.89); C228(1.03); C256(1.28); C266(0.97)  LDD1566  [12]
 LDCM0328  AC64 HEK-293T C88(0.94); C372(0.89); C228(1.01); C256(0.89)  LDD1567  [12]
 LDCM0334  AC7 HEK-293T C88(0.84); C228(1.14); C256(1.30); C266(0.82)  LDD1568  [12]
 LDCM0345  AC8 HEK-293T C88(1.02); C372(1.06); C228(1.07); C256(0.87)  LDD1569  [12]
 LDCM0248  AKOS034007472 HEK-293T C88(1.06); C372(1.50); C228(0.94); C256(0.94)  LDD1511  [12]
 LDCM0356  AKOS034007680 HEK-293T C88(0.92); C228(1.31); C256(0.89); C266(1.06)  LDD1570  [12]
 LDCM0275  AKOS034007705 HEK-293T C88(0.98); C372(1.03); C228(0.98); C256(0.97)  LDD1514  [12]
 LDCM0498  BS-3668 MDA-MB-231 C153(0.79)  LDD2091  [4]
 LDCM0108  Chloroacetamide HeLa H250(0.00); H247(0.00)  LDD0222  [5]
 LDCM0367  CL1 HEK-293T C88(1.02); C228(1.11); C266(1.12)  LDD1571  [12]
 LDCM0368  CL10 HEK-293T C88(0.98); C372(0.60); C228(0.90); C256(0.93)  LDD1572  [12]
 LDCM0369  CL100 HEK-293T C88(0.94); C228(1.17); C256(0.92); C266(1.00)  LDD1573  [12]
 LDCM0370  CL101 HEK-293T C88(1.02); C228(1.05); C266(1.04)  LDD1574  [12]
 LDCM0371  CL102 HEK-293T C88(0.97); C372(0.90); C228(1.09); C256(0.80)  LDD1575  [12]
 LDCM0372  CL103 HEK-293T C88(0.87); C372(1.13); C228(1.12); C256(0.85)  LDD1576  [12]
 LDCM0373  CL104 HEK-293T C88(0.94); C228(1.12); C256(0.97); C266(1.02)  LDD1577  [12]
 LDCM0374  CL105 HEK-293T C88(1.02); C228(0.93); C266(1.13)  LDD1578  [12]
 LDCM0375  CL106 HEK-293T C88(1.10); C372(0.97); C228(1.05); C256(0.96)  LDD1579  [12]
 LDCM0376  CL107 HEK-293T C88(0.91); C372(1.04); C228(0.98); C256(1.03)  LDD1580  [12]
 LDCM0377  CL108 HEK-293T C88(0.91); C228(1.11); C256(1.01); C266(1.25)  LDD1581  [12]
 LDCM0378  CL109 HEK-293T C88(0.93); C228(1.09); C266(0.89)  LDD1582  [12]
 LDCM0379  CL11 HEK-293T C88(1.00); C228(1.00); C256(0.94); C266(0.88)  LDD1583  [12]
 LDCM0380  CL110 HEK-293T C88(1.05); C372(0.64); C228(1.04); C256(0.91)  LDD1584  [12]
 LDCM0381  CL111 HEK-293T C88(0.90); C372(0.93); C228(1.01); C256(1.14)  LDD1585  [12]
 LDCM0382  CL112 HEK-293T C88(0.87); C228(1.18); C256(1.11); C266(0.70)  LDD1586  [12]
 LDCM0383  CL113 HEK-293T C88(1.06); C228(1.11); C266(1.11)  LDD1587  [12]
 LDCM0384  CL114 HEK-293T C88(1.00); C372(0.85); C228(1.09); C256(0.93)  LDD1588  [12]
 LDCM0385  CL115 HEK-293T C88(0.98); C372(1.01); C228(0.98); C256(1.04)  LDD1589  [12]
 LDCM0386  CL116 HEK-293T C88(0.91); C228(1.14); C256(1.09); C266(0.97)  LDD1590  [12]
 LDCM0387  CL117 HEK-293T C88(1.02); C228(0.98); C266(0.93)  LDD1591  [12]
 LDCM0388  CL118 HEK-293T C88(1.11); C372(1.00); C228(1.04); C256(1.12)  LDD1592  [12]
 LDCM0389  CL119 HEK-293T C88(0.98); C372(1.27); C228(1.06); C256(0.97)  LDD1593  [12]
 LDCM0390  CL12 HEK-293T C88(1.03); C372(1.05); C228(0.85); C256(1.14)  LDD1594  [12]
 LDCM0391  CL120 HEK-293T C88(1.04); C228(1.06); C256(0.86); C266(1.20)  LDD1595  [12]
 LDCM0392  CL121 HEK-293T C88(0.97); C228(1.09); C266(1.08)  LDD1596  [12]
 LDCM0393  CL122 HEK-293T C88(0.98); C372(1.10); C228(1.02); C256(0.86)  LDD1597  [12]
 LDCM0394  CL123 HEK-293T C88(0.93); C372(0.85); C228(0.44); C256(1.11)  LDD1598  [12]
 LDCM0395  CL124 HEK-293T C88(1.03); C228(1.12); C256(0.93); C266(0.97)  LDD1599  [12]
 LDCM0396  CL125 HEK-293T C88(1.02); C228(1.06); C266(1.01)  LDD1600  [12]
 LDCM0397  CL126 HEK-293T C88(1.09); C372(0.88); C228(0.99); C256(1.17)  LDD1601  [12]
 LDCM0398  CL127 HEK-293T C88(0.89); C372(1.03); C228(1.10); C256(1.30)  LDD1602  [12]
 LDCM0399  CL128 HEK-293T C88(0.94); C228(1.11); C256(1.01); C266(0.99)  LDD1603  [12]
 LDCM0400  CL13 HEK-293T C88(1.00); C228(0.96); C266(1.23)  LDD1604  [12]
 LDCM0401  CL14 HEK-293T C88(0.93); C372(0.83); C228(1.14); C256(0.99)  LDD1605  [12]
 LDCM0402  CL15 HEK-293T C88(0.87); C372(0.90); C228(1.12); C256(0.79)  LDD1606  [12]
 LDCM0403  CL16 HEK-293T C88(0.99); C228(1.20); C256(1.03); C266(1.01)  LDD1607  [12]
 LDCM0404  CL17 HEK-293T C88(0.90); C228(1.02); C256(0.83); C266(0.82)  LDD1608  [12]
 LDCM0405  CL18 HEK-293T C88(0.99); C372(0.75); C228(0.98); C256(1.20)  LDD1609  [12]
 LDCM0406  CL19 HEK-293T C88(1.33); C372(0.89); C228(1.13); C256(1.00)  LDD1610  [12]
 LDCM0407  CL2 HEK-293T C88(0.96); C372(0.81); C228(1.03); C256(1.14)  LDD1611  [12]
 LDCM0408  CL20 HEK-293T C88(1.17); C228(1.03); C256(0.94); C266(1.05)  LDD1612  [12]
 LDCM0409  CL21 HEK-293T C88(0.93); C372(1.39); C228(0.93); C256(0.92)  LDD1613  [12]
 LDCM0410  CL22 HEK-293T C88(0.98); C372(1.04); C228(0.92); C256(0.86)  LDD1614  [12]
 LDCM0411  CL23 HEK-293T C88(1.04); C228(1.01); C256(0.93); C266(0.97)  LDD1615  [12]
 LDCM0412  CL24 HEK-293T C88(1.10); C372(1.02); C228(0.79); C256(0.89)  LDD1616  [12]
 LDCM0413  CL25 HEK-293T C88(0.96); C228(1.04); C266(1.00)  LDD1617  [12]
 LDCM0414  CL26 HEK-293T C88(1.08); C372(0.80); C228(1.03); C256(0.90)  LDD1618  [12]
 LDCM0415  CL27 HEK-293T C88(0.89); C372(1.07); C228(1.09); C256(0.80)  LDD1619  [12]
 LDCM0416  CL28 HEK-293T C88(1.03); C228(1.02); C256(1.00); C266(1.14)  LDD1620  [12]
 LDCM0417  CL29 HEK-293T C88(1.04); C228(1.25); C256(0.96); C266(1.07)  LDD1621  [12]
 LDCM0418  CL3 HEK-293T C88(0.94); C372(0.88); C228(1.11); C256(1.15)  LDD1622  [12]
 LDCM0419  CL30 HEK-293T C88(0.91); C372(0.80); C228(0.96); C256(0.79)  LDD1623  [12]
 LDCM0420  CL31 HEK-293T C88(1.20); C372(0.92); C228(1.07); C256(0.85)  LDD1624  [12]
 LDCM0421  CL32 HEK-293T C88(0.97); C228(0.97); C256(1.01); C266(1.14)  LDD1625  [12]
 LDCM0422  CL33 HEK-293T C88(0.99); C372(0.79); C228(0.88); C256(0.83)  LDD1626  [12]
 LDCM0423  CL34 HEK-293T C88(1.03); C372(0.55); C228(0.95); C256(1.20)  LDD1627  [12]
 LDCM0424  CL35 HEK-293T C88(0.90); C228(0.96); C256(1.21); C266(0.86)  LDD1628  [12]
 LDCM0425  CL36 HEK-293T C88(1.14); C372(1.26); C228(0.85); C256(0.93)  LDD1629  [12]
 LDCM0426  CL37 HEK-293T C88(1.12); C228(0.97); C266(0.98)  LDD1630  [12]
 LDCM0428  CL39 HEK-293T C88(0.92); C372(0.90); C228(0.86); C256(0.88)  LDD1632  [12]
 LDCM0429  CL4 HEK-293T C88(0.91); C228(1.16); C256(0.78); C266(1.23)  LDD1633  [12]
 LDCM0430  CL40 HEK-293T C88(0.87); C228(0.92); C256(0.97); C266(1.16)  LDD1634  [12]
 LDCM0431  CL41 HEK-293T C88(0.87); C228(1.13); C256(0.97); C266(0.90)  LDD1635  [12]
 LDCM0432  CL42 HEK-293T C88(1.00); C372(0.96); C228(0.92); C256(1.05)  LDD1636  [12]
 LDCM0433  CL43 HEK-293T C88(1.33); C372(0.78); C228(0.99); C256(1.22)  LDD1637  [12]
 LDCM0434  CL44 HEK-293T C88(0.86); C228(0.85); C256(0.96); C266(1.18)  LDD1638  [12]
 LDCM0435  CL45 HEK-293T C88(1.21); C372(1.00); C228(0.87); C256(1.01)  LDD1639  [12]
 LDCM0436  CL46 HEK-293T C88(1.05); C372(0.99); C228(0.86); C256(0.95)  LDD1640  [12]
 LDCM0437  CL47 HEK-293T C88(1.05); C228(0.99); C256(1.04); C266(0.75)  LDD1641  [12]
 LDCM0438  CL48 HEK-293T C88(0.92); C372(1.19); C228(0.93); C256(0.94)  LDD1642  [12]
 LDCM0439  CL49 HEK-293T C88(1.02); C228(1.06); C266(1.07)  LDD1643  [12]
 LDCM0440  CL5 HEK-293T C88(1.01); C228(1.17); C256(1.13); C266(0.99)  LDD1644  [12]
 LDCM0441  CL50 HEK-293T C88(0.86); C372(0.81); C228(0.87); C256(1.05)  LDD1645  [12]
 LDCM0443  CL52 HEK-293T C88(0.98); C228(1.09); C256(0.95); C266(0.99)  LDD1646  [12]
 LDCM0444  CL53 HEK-293T C88(0.95); C228(1.09); C256(0.87); C266(0.96)  LDD1647  [12]
 LDCM0445  CL54 HEK-293T C88(1.15); C372(0.56); C228(0.79); C256(0.84)  LDD1648  [12]
 LDCM0446  CL55 HEK-293T C88(1.13); C372(0.94); C228(1.09); C256(0.96)  LDD1649  [12]
 LDCM0447  CL56 HEK-293T C88(1.10); C228(0.98); C256(0.83); C266(0.59)  LDD1650  [12]
 LDCM0448  CL57 HEK-293T C88(1.14); C372(1.35); C228(1.00); C256(0.91)  LDD1651  [12]
 LDCM0449  CL58 HEK-293T C88(0.99); C372(0.50); C228(0.84); C256(0.68)  LDD1652  [12]
 LDCM0450  CL59 HEK-293T C88(1.01); C228(1.15); C256(0.90); C266(0.90)  LDD1653  [12]
 LDCM0451  CL6 HEK-293T C88(1.22); C372(0.81); C228(1.00); C256(0.88)  LDD1654  [12]
 LDCM0452  CL60 HEK-293T C88(0.95); C372(1.67); C228(0.85); C256(0.81)  LDD1655  [12]
 LDCM0453  CL61 HEK-293T C88(1.02); C228(1.08); C266(1.13)  LDD1656  [12]
 LDCM0454  CL62 HEK-293T C88(1.06); C372(0.81); C228(0.97); C256(1.37)  LDD1657  [12]
 LDCM0455  CL63 HEK-293T C88(0.92); C372(1.10); C228(1.18); C256(0.95)  LDD1658  [12]
 LDCM0456  CL64 HEK-293T C88(0.89); C228(1.03); C256(0.92); C266(0.82)  LDD1659  [12]
 LDCM0457  CL65 HEK-293T C88(0.90); C228(1.11); C256(1.23); C266(1.09)  LDD1660  [12]
 LDCM0458  CL66 HEK-293T C88(1.15); C372(0.55); C228(0.99); C256(1.25)  LDD1661  [12]
 LDCM0459  CL67 HEK-293T C88(1.27); C372(0.93); C228(1.11); C256(0.92)  LDD1662  [12]
 LDCM0460  CL68 HEK-293T C88(1.13); C228(0.88); C256(0.98); C266(1.22)  LDD1663  [12]
 LDCM0461  CL69 HEK-293T C88(1.02); C372(1.25); C228(0.94); C256(0.94)  LDD1664  [12]
 LDCM0462  CL7 HEK-293T C88(1.28); C372(0.91); C228(1.08); C256(1.20)  LDD1665  [12]
 LDCM0463  CL70 HEK-293T C88(1.01); C372(1.11); C228(0.97); C256(0.94)  LDD1666  [12]
 LDCM0464  CL71 HEK-293T C88(0.94); C228(1.03); C256(0.85); C266(0.99)  LDD1667  [12]
 LDCM0465  CL72 HEK-293T C88(1.04); C372(1.41); C228(0.86); C256(0.71)  LDD1668  [12]
 LDCM0466  CL73 HEK-293T C88(0.97); C228(1.02); C266(1.14)  LDD1669  [12]
 LDCM0467  CL74 HEK-293T C88(1.03); C372(0.85); C228(1.19); C256(0.95)  LDD1670  [12]
 LDCM0469  CL76 HEK-293T C88(0.90); C228(0.95); C256(0.98); C266(0.89)  LDD1672  [12]
 LDCM0470  CL77 HEK-293T C88(0.87); C228(0.98); C256(0.67); C266(0.91)  LDD1673  [12]
 LDCM0471  CL78 HEK-293T C88(1.01); C372(0.92); C228(1.21); C256(1.25)  LDD1674  [12]
 LDCM0472  CL79 HEK-293T C88(1.15); C372(0.78); C228(1.13); C256(1.08)  LDD1675  [12]
 LDCM0473  CL8 HEK-293T C88(0.98); C228(0.84); C256(0.76); C266(0.77)  LDD1676  [12]
 LDCM0474  CL80 HEK-293T C88(1.15); C228(1.13); C256(1.15); C266(1.16)  LDD1677  [12]
 LDCM0475  CL81 HEK-293T C88(1.12); C372(1.45); C228(0.94); C256(1.02)  LDD1678  [12]
 LDCM0476  CL82 HEK-293T C88(0.97); C372(1.16); C228(0.95); C256(0.94)  LDD1679  [12]
 LDCM0477  CL83 HEK-293T C88(1.14); C228(1.18); C256(0.94); C266(1.01)  LDD1680  [12]
 LDCM0478  CL84 HEK-293T C88(1.05); C372(0.98); C228(0.99); C256(1.14)  LDD1681  [12]
 LDCM0479  CL85 HEK-293T C88(1.07); C228(1.08); C266(1.00)  LDD1682  [12]
 LDCM0480  CL86 HEK-293T C88(1.03); C372(1.26); C228(0.96); C256(1.16)  LDD1683  [12]
 LDCM0481  CL87 HEK-293T C88(0.94); C372(1.16); C228(0.96); C256(1.23)  LDD1684  [12]
 LDCM0482  CL88 HEK-293T C88(1.02); C228(1.04); C256(0.95); C266(0.96)  LDD1685  [12]
 LDCM0483  CL89 HEK-293T C88(0.80); C228(1.06); C256(0.78); C266(1.08)  LDD1686  [12]
 LDCM0484  CL9 HEK-293T C88(1.18); C372(1.46); C228(1.08); C256(1.05)  LDD1687  [12]
 LDCM0485  CL90 HEK-293T C88(0.87); C372(0.54); C228(1.04); C256(0.84)  LDD1688  [12]
 LDCM0486  CL91 HEK-293T C88(1.24); C372(0.99); C228(1.03); C256(0.97)  LDD1689  [12]
 LDCM0487  CL92 HEK-293T C88(0.90); C228(1.10); C256(0.96); C266(0.92)  LDD1690  [12]
 LDCM0488  CL93 HEK-293T C88(1.07); C372(1.11); C228(0.90); C256(0.93)  LDD1691  [12]
 LDCM0489  CL94 HEK-293T C88(0.97); C372(1.09); C228(0.94); C256(1.04)  LDD1692  [12]
 LDCM0490  CL95 HEK-293T C88(0.90); C228(1.17); C256(0.89); C266(0.72)  LDD1693  [12]
 LDCM0491  CL96 HEK-293T C88(1.15); C372(1.35); C228(0.95); C256(0.93)  LDD1694  [12]
 LDCM0492  CL97 HEK-293T C88(0.99); C228(0.95); C266(0.92)  LDD1695  [12]
 LDCM0493  CL98 HEK-293T C88(0.95); C372(0.80); C228(1.14); C256(0.83)  LDD1696  [12]
 LDCM0494  CL99 HEK-293T C88(0.93); C372(1.12); C228(1.00); C256(1.48)  LDD1697  [12]
 LDCM0495  E2913 HEK-293T C88(0.94); C372(0.89); C228(0.96); C256(1.42)  LDD1698  [12]
 LDCM0468  Fragment33 HEK-293T C88(0.96); C372(0.97); C228(1.09); C256(0.74)  LDD1671  [12]
 LDCM0427  Fragment51 HEK-293T C88(1.03); C372(0.91); C228(1.09); C256(0.86)  LDD1631  [12]
 LDCM0107  IAA HeLa H250(0.00); H247(0.00)  LDD0221  [5]
 LDCM0022  KB02 HEK-293T C228(1.20); C256(0.99); C266(0.97); C372(0.81)  LDD1492  [12]
 LDCM0023  KB03 HEK-293T C228(0.88); C256(1.16); C266(1.06); C372(0.76)  LDD1497  [12]
 LDCM0024  KB05 HMCB C88(1.95)  LDD3312  [3]
 LDCM0109  NEM HeLa H250(0.00); H247(0.00)  LDD0223  [5]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C153(0.87)  LDD2093  [4]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C153(0.22)  LDD2096  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C153(1.05)  LDD2107  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C153(0.94)  LDD2109  [4]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C153(0.38)  LDD2116  [4]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C153(0.30)  LDD2118  [4]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C153(1.66)  LDD2119  [4]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C153(0.88)  LDD2120  [4]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C153(0.35)  LDD2122  [4]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C153(0.65)  LDD2123  [4]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C153(0.33)  LDD2124  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C153(1.17)  LDD2127  [4]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C153(0.91)  LDD2128  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C153(0.98)  LDD2135  [4]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C153(0.99)  LDD2137  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C153(0.89)  LDD2140  [4]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C153(0.87)  LDD2143  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C153(2.53)  LDD2144  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C153(0.84)  LDD2146  [4]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C153(0.42)  LDD2149  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C153(0.66)  LDD2150  [4]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C153(0.34)  LDD2151  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Superoxide dismutase [Cu-Zn] (SOD1) Cu-Zn superoxide dismutase family P00441
Damage-control phosphatase ARMT1 (ARMT1) Damage-control phosphatase family Q9H993
Inositol-trisphosphate 3-kinase B (ITPKB) Inositol phosphokinase (IPK) family P27987
Ubiquitin carboxyl-terminal hydrolase 14 (USP14) Peptidase C19 family P54578
Tensin-4 (TNS4) PTEN phosphatase protein family Q8IZW8
Utrophin (UTRN) . P46939
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Tripartite motif-containing protein 72 (TRIM72) TRIM/RBCC family Q6ZMU5
A-kinase anchor protein 5 (AKAP5) . P24588
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nuclear factor of activated T-cells, cytoplasmic 1 (NFATC1) . O95644
Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2) . Q13469
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calcineurin subunit B type 1 (PPP3R1) Calcineurin regulatory subunit family P63098
Protein FAM167A (FAM167A) FAM167 (SEC) family Q96KS9
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993
Protein unc-119 homolog A (UNC119) PDE6D/unc-119 family Q13432
Amphiphysin (AMPH) . P49418
Uncharacterized protein C16orf74 (C16orf74) . Q96GX8

The Drug(s) Related To This Target

Approved
Click To Hide/Show 4 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Ciclosporin Protein/peptide D0O3YF
Pimecrolimus Small molecular drug D0Z4UN
Tacrolimus Small molecular drug D06OMK
Voclosporin Small molecular drug D0L9HX
Investigative
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
L-685,818 Small molecular drug D08IGF
Myristic Acid Small molecular drug DB08231

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
11 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402