Details of the Target

General Information of Target

Target ID LDTP11944
Target Name mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase (PCIF1)
Gene Name PCIF1
Gene ID 63935
Synonyms
C20orf67; CAPAM; PPP1R121; mRNA; 2'-O-methyladenosine-N(6)-)-methyltransferase; EC 2.1.1.62; Cap-specific adenosine methyltransferase; CAPAM; hCAPAM; Phosphorylated CTD-interacting factor 1; hPCIF1; Protein phosphatase 1 regulatory subunit 121
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEENEVESSSDAAPGPGRPEEPSESGLGVGTSEAVSADSSDAAAAPGQAEADDSGVGQSS
DRGSRSQEEVSESSSSADPLPNSYLPDSSSVSHGPVAGVTGGPPALVHSSALPDPNMLVS
DCTASSSDLGSAIDKIIESTIGPDLIQNCITVTSAEDGGAETTRYLILQGPDDGAPMTSP
MSSSTLAHSLAAIEALADGPTSTSTCLEAQGGPSSPVQLPPASGAEEPDLQSLEAMMEVV
VVQQFKCKMCQYRSSTKATLLRHMRERHFRPVAAAAAAAGKKGRLRKWSTSTKSQEEEGP
EEEDDDDIVDAGAIDDLEEDSDYNPAEDEPRGRQLRLQRPTPSTPRPRRRPGRPRKLPRL
EISDLPDGVEGEPLVSSQSGQSPPEPQDPEAPSSSGPGHLVAMGKVSRTPVEAGVSQSDA
ENAAPSCPDEHDTLPRRRGRPSRRFLGKKYRKYYYKSPKPLLRPFLCRICGSRFLSHEDL
RFHVNSHEAGDPQLFKCLQCSYRSRRWSSLKEHMFNHVGSKPYKCDECSYTSVYRKDVIR
HAAVHSRDRKKRPDPTPKLSSFPCPVCGRVYPMQKRLTQHMKTHSTEKPHMCDKCGKSFK
KRYTFKMHLLTHIQAVANRRFKCEFCEFVCEDKKALLNHQLSHVSDKPFKCSFCPYRTFR
EDFLLSHVAVKHTGAKPFACEYCHFSTRHKKNLRLHVRCRHASSFEEWGRRHPEEPPSRR
RPFFSLQQIEELKQQHSAAPGPPPSSPGPPEIPPEATTFQSSEAPSLLCSDTLGGATIIY
QQGAEESTAMATQTALDLLLNMSAQRELGGTALQVAVVKSEDVEAGLASPGGQPSPEGAT
PQVVTLHVAEPGGGAAAESQLGPPDLPQITLAPGPFGGTGYSVITAPPMEEGTSAPGTPY
SEEPAGEAAQAVVVSDTLKEAGTHYIMATDGTQLHHIELTADGSISFPSPDALASGAKWP
LLQCGGLPRDGPEPPSPAKTHCVGDSQSSASSPPATSKALGLAVPPSPPSAATAASKKFS
CKICAEAFPGRAEMESHKRAHAGPGAFKCPDCPFSARQWPEVRAHMAQHSSLRPHQCSQC
SFASKNKKDLRRHMLTHTKEKPFACHLCGQRFNRNGHLKFHIQRLHSPDGRKSGTPTARA
PTQTPTQTIILNSDDETLATLHTALQSSHGVLGPERLQQALSQEHIIVAQEQTVTNQEEA
AYIQEITTADGQTVQHLVTSDNQVQYIISQDGVQHLLPQEYVVVPEGHHIQVQEGQITHI
QYEQGAPFLQESQIQYVPVSPGQQLVTQAQLEAAAHSAVTAVADAAMAQAQGLFGTDETV
PEHIQQLQHQGIEYDVITLADD
Target Bioclass
Enzyme
Family
CAPAM family
Subcellular location
Nucleus
Function
Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally.
Uniprot ID
Q9H4Z3
Ensemble ID
ENST00000372409.8
HGNC ID
HGNC:16200

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K285(2.34); K290(8.47)  LDD0277  [1]
IPM
 Probe Info  		N.A.  LDD0241  [2]
Probe 1
 Probe Info  		Y453(19.12); Y667(9.88)  LDD3495  [3]
BTD
 Probe Info  		C345(0.62)  LDD2089  [4]
AHL-Pu-1
 Probe Info  		C557(10.07)  LDD0170  [5]
DBIA
 Probe Info  		C361(1.32)  LDD0080  [6]
5E-2FA
 Probe Info  		N.A.  LDD2235  [7]
m-APA
 Probe Info  		N.A.  LDD2231  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		C361(0.00); C255(0.00); C524(0.00); C455(0.00)  LDD0038  [8]
IA-alkyne
 Probe Info  		C361(0.00); C404(0.00); C255(0.00); C524(0.00)  LDD0036  [8]
Lodoacetamide azide
 Probe Info  		C361(0.00); C404(0.00); C524(0.00); C455(0.00)  LDD0037  [8]
NAIA_4
 Probe Info  		N.A.  LDD2226  [9]
TFBX
 Probe Info  		N.A.  LDD0148  [10]
Acrolein
 Probe Info  		N.A.  LDD0217  [11]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [11]
W1
 Probe Info  		C404(0.00); C255(0.00)  LDD0236  [2]
NAIA_5
 Probe Info  		C404(0.00); C345(0.00); C626(0.00)  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C345(0.43)  LDD2131  [4]
 LDCM0025  4SU-RNA DM93 C557(10.07)  LDD0170  [5]
 LDCM0214  AC1 HEK-293T C540(1.40); C557(0.87)  LDD0812  [6]
 LDCM0215  AC10 HEK-293T C540(0.52); C557(0.93)  LDD0813  [6]
 LDCM0216  AC100 HEK-293T C557(1.10)  LDD0814  [6]
 LDCM0217  AC101 HEK-293T C557(0.97)  LDD0815  [6]
 LDCM0218  AC102 HEK-293T C557(0.94)  LDD0816  [6]
 LDCM0219  AC103 HEK-293T C557(1.18)  LDD0817  [6]
 LDCM0220  AC104 HEK-293T C557(1.30)  LDD0818  [6]
 LDCM0221  AC105 HEK-293T C557(0.86)  LDD0819  [6]
 LDCM0222  AC106 HEK-293T C557(0.81)  LDD0820  [6]
 LDCM0223  AC107 HEK-293T C557(1.14)  LDD0821  [6]
 LDCM0224  AC108 HEK-293T C557(1.00)  LDD0822  [6]
 LDCM0225  AC109 HEK-293T C557(0.96)  LDD0823  [6]
 LDCM0226  AC11 HEK-293T C540(0.65); C557(0.83)  LDD0824  [6]
 LDCM0227  AC110 HEK-293T C557(1.25)  LDD0825  [6]
 LDCM0228  AC111 HEK-293T C557(1.50)  LDD0826  [6]
 LDCM0229  AC112 HEK-293T C557(1.36)  LDD0827  [6]
 LDCM0230  AC113 HEK-293T C557(1.24)  LDD0828  [6]
 LDCM0231  AC114 HEK-293T C557(1.20)  LDD0829  [6]
 LDCM0232  AC115 HEK-293T C557(1.19)  LDD0830  [6]
 LDCM0233  AC116 HEK-293T C557(1.10)  LDD0831  [6]
 LDCM0234  AC117 HEK-293T C557(1.10)  LDD0832  [6]
 LDCM0235  AC118 HEK-293T C557(1.28)  LDD0833  [6]
 LDCM0236  AC119 HEK-293T C557(1.23)  LDD0834  [6]
 LDCM0237  AC12 HEK-293T C540(0.93); C557(0.80)  LDD0835  [6]
 LDCM0238  AC120 HEK-293T C557(1.29)  LDD0836  [6]
 LDCM0239  AC121 HEK-293T C557(1.46)  LDD0837  [6]
 LDCM0240  AC122 HEK-293T C557(1.59)  LDD0838  [6]
 LDCM0241  AC123 HEK-293T C557(1.40)  LDD0839  [6]
 LDCM0242  AC124 HEK-293T C557(1.22)  LDD0840  [6]
 LDCM0243  AC125 HEK-293T C557(1.50)  LDD0841  [6]
 LDCM0244  AC126 HEK-293T C557(2.02)  LDD0842  [6]
 LDCM0245  AC127 HEK-293T C557(1.82)  LDD0843  [6]
 LDCM0246  AC128 HEK-293T C455(1.10); C557(0.97)  LDD0844  [6]
 LDCM0247  AC129 HEK-293T C455(1.05); C557(0.98)  LDD0845  [6]
 LDCM0249  AC130 HEK-293T C455(0.99); C557(0.92)  LDD0847  [6]
 LDCM0250  AC131 HEK-293T C455(0.87); C557(0.86)  LDD0848  [6]
 LDCM0251  AC132 HEK-293T C455(0.97); C557(1.02)  LDD0849  [6]
 LDCM0252  AC133 HEK-293T C455(1.26); C557(1.11)  LDD0850  [6]
 LDCM0253  AC134 HEK-293T C455(0.97); C557(0.99)  LDD0851  [6]
 LDCM0254  AC135 HEK-293T C455(1.19); C557(1.21)  LDD0852  [6]
 LDCM0255  AC136 HEK-293T C455(1.12); C557(1.05)  LDD0853  [6]
 LDCM0256  AC137 HEK-293T C455(1.28); C557(1.32)  LDD0854  [6]
 LDCM0257  AC138 HEK-293T C455(1.09); C557(1.50)  LDD0855  [6]
 LDCM0258  AC139 HEK-293T C455(1.08); C557(1.31)  LDD0856  [6]
 LDCM0259  AC14 HEK-293T C540(0.47); C557(0.92)  LDD0857  [6]
 LDCM0260  AC140 HEK-293T C455(1.05); C557(1.08)  LDD0858  [6]
 LDCM0261  AC141 HEK-293T C455(1.08); C557(1.18)  LDD0859  [6]
 LDCM0262  AC142 HEK-293T C455(0.98); C557(1.11)  LDD0860  [6]
 LDCM0263  AC143 HEK-293T C540(0.64); C557(0.96)  LDD0861  [6]
 LDCM0264  AC144 HEK-293T C540(1.01); C557(1.04)  LDD0862  [6]
 LDCM0265  AC145 HEK-293T C540(0.96); C557(1.10)  LDD0863  [6]
 LDCM0266  AC146 HEK-293T C540(1.11); C557(1.36)  LDD0864  [6]
 LDCM0267  AC147 HEK-293T C540(0.97); C557(1.06)  LDD0865  [6]
 LDCM0268  AC148 HEK-293T C540(0.83); C557(1.52)  LDD0866  [6]
 LDCM0269  AC149 HEK-293T C540(0.65); C557(1.00)  LDD0867  [6]
 LDCM0270  AC15 HEK-293T C540(0.67); C557(1.22)  LDD0868  [6]
 LDCM0271  AC150 HEK-293T C540(0.82); C557(1.07)  LDD0869  [6]
 LDCM0272  AC151 HEK-293T C540(0.85); C557(0.98)  LDD0870  [6]
 LDCM0273  AC152 HEK-293T C540(0.82); C557(1.09)  LDD0871  [6]
 LDCM0274  AC153 HEK-293T C540(0.93); C557(0.73)  LDD0872  [6]
 LDCM0621  AC154 HEK-293T C540(0.91); C557(0.93)  LDD2162  [6]
 LDCM0622  AC155 HEK-293T C540(1.12); C557(0.99)  LDD2163  [6]
 LDCM0623  AC156 HEK-293T C540(0.97); C557(1.48)  LDD2164  [6]
 LDCM0624  AC157 HEK-293T C540(1.08); C557(1.08)  LDD2165  [6]
 LDCM0276  AC17 HEK-293T C540(1.43); C557(1.07)  LDD0874  [6]
 LDCM0277  AC18 HEK-293T C540(1.22); C557(1.20)  LDD0875  [6]
 LDCM0278  AC19 HEK-293T C540(1.31); C557(0.95)  LDD0876  [6]
 LDCM0279  AC2 HEK-293T C540(0.94); C557(1.25)  LDD0877  [6]
 LDCM0280  AC20 HEK-293T C540(1.84); C557(1.11)  LDD0878  [6]
 LDCM0281  AC21 HEK-293T C540(1.28); C557(0.88)  LDD0879  [6]
 LDCM0282  AC22 HEK-293T C540(1.38); C557(1.04)  LDD0880  [6]
 LDCM0283  AC23 HEK-293T C540(1.33); C557(1.25)  LDD0881  [6]
 LDCM0284  AC24 HEK-293T C540(1.42); C557(1.03)  LDD0882  [6]
 LDCM0285  AC25 HEK-293T C540(1.13)  LDD0883  [6]
 LDCM0286  AC26 HEK-293T C540(1.53)  LDD0884  [6]
 LDCM0287  AC27 HEK-293T C540(1.28)  LDD0885  [6]
 LDCM0288  AC28 HEK-293T C540(1.34)  LDD0886  [6]
 LDCM0289  AC29 HEK-293T C540(1.48)  LDD0887  [6]
 LDCM0290  AC3 HEK-293T C540(0.94); C557(0.91)  LDD0888  [6]
 LDCM0291  AC30 HEK-293T C540(1.83)  LDD0889  [6]
 LDCM0292  AC31 HEK-293T C540(1.64)  LDD0890  [6]
 LDCM0293  AC32 HEK-293T C540(1.52)  LDD0891  [6]
 LDCM0294  AC33 HEK-293T C540(1.38)  LDD0892  [6]
 LDCM0295  AC34 HEK-293T C540(1.86)  LDD0893  [6]
 LDCM0296  AC35 HCT 116 C404(1.29)  LDD0613  [6]
 LDCM0297  AC36 HCT 116 C404(2.75)  LDD0614  [6]
 LDCM0298  AC37 HCT 116 C404(1.57)  LDD0615  [6]
 LDCM0299  AC38 HCT 116 C404(1.19)  LDD0616  [6]
 LDCM0300  AC39 HCT 116 C404(1.18)  LDD0617  [6]
 LDCM0301  AC4 HEK-293T C540(0.99); C557(1.05)  LDD0899  [6]
 LDCM0302  AC40 HCT 116 C404(0.88)  LDD0619  [6]
 LDCM0303  AC41 HCT 116 C404(1.20)  LDD0620  [6]
 LDCM0304  AC42 HCT 116 C404(1.64)  LDD0621  [6]
 LDCM0305  AC43 HCT 116 C404(1.06)  LDD0622  [6]
 LDCM0306  AC44 HCT 116 C404(1.28)  LDD0623  [6]
 LDCM0307  AC45 HCT 116 C404(1.11)  LDD0624  [6]
 LDCM0308  AC46 HCT 116 C404(1.27)  LDD0625  [6]
 LDCM0309  AC47 HCT 116 C404(1.18)  LDD0626  [6]
 LDCM0310  AC48 HCT 116 C404(1.05)  LDD0627  [6]
 LDCM0311  AC49 HCT 116 C404(0.72)  LDD0628  [6]
 LDCM0312  AC5 HEK-293T C540(1.54); C557(1.10)  LDD0910  [6]
 LDCM0313  AC50 HCT 116 C404(0.73)  LDD0630  [6]
 LDCM0314  AC51 HCT 116 C404(1.35)  LDD0631  [6]
 LDCM0315  AC52 HCT 116 C404(1.10)  LDD0632  [6]
 LDCM0316  AC53 HCT 116 C404(0.92)  LDD0633  [6]
 LDCM0317  AC54 HCT 116 C404(0.82)  LDD0634  [6]
 LDCM0318  AC55 HCT 116 C404(0.87)  LDD0635  [6]
 LDCM0319  AC56 HCT 116 C404(0.90)  LDD0636  [6]
 LDCM0320  AC57 HEK-293T C540(0.97); C557(1.26)  LDD0918  [6]
 LDCM0321  AC58 HEK-293T C540(1.02); C557(1.31)  LDD0919  [6]
 LDCM0322  AC59 HEK-293T C540(1.41); C557(1.28)  LDD0920  [6]
 LDCM0323  AC6 HEK-293T C540(1.36); C557(0.92)  LDD0921  [6]
 LDCM0324  AC60 HEK-293T C540(1.39); C557(1.18)  LDD0922  [6]
 LDCM0325  AC61 HEK-293T C540(0.99); C557(1.76)  LDD0923  [6]
 LDCM0326  AC62 HEK-293T C540(1.07); C557(1.50)  LDD0924  [6]
 LDCM0327  AC63 HEK-293T C540(1.31); C557(1.61)  LDD0925  [6]
 LDCM0328  AC64 HEK-293T C540(1.24); C557(1.55)  LDD0926  [6]
 LDCM0329  AC65 HEK-293T C540(1.02); C557(1.50)  LDD0927  [6]
 LDCM0330  AC66 HEK-293T C540(0.94); C557(1.45)  LDD0928  [6]
 LDCM0331  AC67 HEK-293T C540(1.26); C557(1.30)  LDD0929  [6]
 LDCM0332  AC68 HCT 116 C404(0.67)  LDD0649  [6]
 LDCM0333  AC69 HCT 116 C404(0.43)  LDD0650  [6]
 LDCM0334  AC7 HEK-293T C540(0.89); C557(1.03)  LDD0932  [6]
 LDCM0335  AC70 HCT 116 C404(0.28)  LDD0652  [6]
 LDCM0336  AC71 HCT 116 C404(0.83)  LDD0653  [6]
 LDCM0337  AC72 HCT 116 C404(0.49)  LDD0654  [6]
 LDCM0338  AC73 HCT 116 C404(0.38)  LDD0655  [6]
 LDCM0339  AC74 HCT 116 C404(0.40)  LDD0656  [6]
 LDCM0340  AC75 HCT 116 C404(0.45)  LDD0657  [6]
 LDCM0341  AC76 HCT 116 C404(0.54)  LDD0658  [6]
 LDCM0342  AC77 HCT 116 C404(0.40)  LDD0659  [6]
 LDCM0343  AC78 HCT 116 C404(0.71)  LDD0660  [6]
 LDCM0344  AC79 HCT 116 C404(0.70)  LDD0661  [6]
 LDCM0345  AC8 HEK-293T C540(1.04); C557(0.94)  LDD0943  [6]
 LDCM0346  AC80 HCT 116 C404(0.49)  LDD0663  [6]
 LDCM0347  AC81 HCT 116 C404(0.76)  LDD0664  [6]
 LDCM0348  AC82 HCT 116 C404(0.42)  LDD0665  [6]
 LDCM0349  AC83 HEK-293T C540(1.03); C455(1.25); C557(0.83)  LDD0947  [6]
 LDCM0350  AC84 HEK-293T C540(1.13); C455(1.40); C557(0.68)  LDD0948  [6]
 LDCM0351  AC85 HEK-293T C540(1.86); C455(0.96); C557(0.77)  LDD0949  [6]
 LDCM0352  AC86 HEK-293T C540(2.17); C455(1.07); C557(0.77)  LDD0950  [6]
 LDCM0353  AC87 HEK-293T C540(0.83); C455(0.91); C557(1.05)  LDD0951  [6]
 LDCM0354  AC88 HEK-293T C540(0.69); C455(1.30); C557(1.05)  LDD0952  [6]
 LDCM0355  AC89 HEK-293T C540(1.33); C455(1.20); C557(0.84)  LDD0953  [6]
 LDCM0357  AC90 HEK-293T C540(1.49); C455(1.08); C557(1.03)  LDD0955  [6]
 LDCM0358  AC91 HEK-293T C540(0.67); C455(1.23); C557(0.88)  LDD0956  [6]
 LDCM0359  AC92 HEK-293T C540(1.50); C455(1.17); C557(1.04)  LDD0957  [6]
 LDCM0360  AC93 HEK-293T C540(1.37); C455(1.06); C557(0.78)  LDD0958  [6]
 LDCM0361  AC94 HEK-293T C540(1.10); C455(1.24); C557(0.84)  LDD0959  [6]
 LDCM0362  AC95 HEK-293T C540(1.02); C455(1.33); C557(0.79)  LDD0960  [6]
 LDCM0363  AC96 HEK-293T C540(1.80); C455(0.98); C557(1.05)  LDD0961  [6]
 LDCM0364  AC97 HEK-293T C540(1.72); C455(1.49); C557(1.17)  LDD0962  [6]
 LDCM0365  AC98 HEK-293T C557(0.93)  LDD0963  [6]
 LDCM0366  AC99 HEK-293T C557(1.05)  LDD0964  [6]
 LDCM0248  AKOS034007472 HEK-293T C540(0.70); C557(0.79)  LDD0846  [6]
 LDCM0356  AKOS034007680 HEK-293T C540(0.65); C557(1.16)  LDD0954  [6]
 LDCM0275  AKOS034007705 HEK-293T C540(0.54); C557(0.90)  LDD0873  [6]
 LDCM0498  BS-3668 MDA-MB-231 C345(0.47)  LDD2091  [4]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [11]
 LDCM0632  CL-Sc Hep-G2 C626(20.00); C29(0.41)  LDD2227  [9]
 LDCM0367  CL1 HCT 116 C404(0.76)  LDD0684  [6]
 LDCM0368  CL10 HCT 116 C404(0.74)  LDD0685  [6]
 LDCM0369  CL100 HEK-293T C540(1.04); C557(1.05)  LDD0967  [6]
 LDCM0370  CL101 HEK-293T C540(0.89); C557(1.09)  LDD0968  [6]
 LDCM0371  CL102 HEK-293T C540(0.91); C557(0.95)  LDD0969  [6]
 LDCM0372  CL103 HEK-293T C540(0.74); C557(0.99)  LDD0970  [6]
 LDCM0373  CL104 HEK-293T C540(0.73); C557(0.91)  LDD0971  [6]
 LDCM0374  CL105 HEK-293T C540(1.55); C557(0.89)  LDD0972  [6]
 LDCM0375  CL106 HEK-293T C540(0.97); C557(1.04)  LDD0973  [6]
 LDCM0376  CL107 HEK-293T C540(1.31); C557(0.94)  LDD0974  [6]
 LDCM0377  CL108 HEK-293T C540(0.91); C557(0.96)  LDD0975  [6]
 LDCM0378  CL109 HEK-293T C540(1.36); C557(1.08)  LDD0976  [6]
 LDCM0379  CL11 HCT 116 C404(0.71)  LDD0696  [6]
 LDCM0380  CL110 HEK-293T C540(1.18); C557(1.14)  LDD0978  [6]
 LDCM0381  CL111 HEK-293T C540(2.28); C557(0.88)  LDD0979  [6]
 LDCM0382  CL112 HEK-293T C540(1.38)  LDD0980  [6]
 LDCM0383  CL113 HEK-293T C540(1.50)  LDD0981  [6]
 LDCM0384  CL114 HEK-293T C540(1.77)  LDD0982  [6]
 LDCM0385  CL115 HEK-293T C540(1.88)  LDD0983  [6]
 LDCM0386  CL116 HEK-293T C540(1.81)  LDD0984  [6]
 LDCM0387  CL117 HCT 116 C404(0.85)  LDD0704  [6]
 LDCM0388  CL118 HCT 116 C404(1.10)  LDD0705  [6]
 LDCM0389  CL119 HCT 116 C404(1.14)  LDD0706  [6]
 LDCM0390  CL12 HCT 116 C404(0.70)  LDD0707  [6]
 LDCM0391  CL120 HCT 116 C404(1.58)  LDD0708  [6]
 LDCM0392  CL121 HCT 116 C404(0.80)  LDD0709  [6]
 LDCM0393  CL122 HCT 116 C404(1.06)  LDD0710  [6]
 LDCM0394  CL123 HCT 116 C404(0.79)  LDD0711  [6]
 LDCM0395  CL124 HCT 116 C404(0.69)  LDD0712  [6]
 LDCM0396  CL125 HEK-293T C540(1.47); C557(0.99)  LDD0994  [6]
 LDCM0397  CL126 HEK-293T C540(0.76); C557(1.69)  LDD0995  [6]
 LDCM0398  CL127 HEK-293T C540(1.21); C557(1.22)  LDD0996  [6]
 LDCM0399  CL128 HEK-293T C540(1.17); C557(1.18)  LDD0997  [6]
 LDCM0400  CL13 HCT 116 C404(0.98)  LDD0717  [6]
 LDCM0401  CL14 HCT 116 C404(0.97)  LDD0718  [6]
 LDCM0402  CL15 HCT 116 C404(0.88)  LDD0719  [6]
 LDCM0403  CL16 PaTu 8988t C404(0.40); C540(0.84); C557(0.84)  LDD1282  [6]
 LDCM0404  CL17 PaTu 8988t C404(0.28); C540(1.33); C557(1.33)  LDD1283  [6]
 LDCM0405  CL18 PaTu 8988t C404(0.28); C540(0.34); C557(0.34)  LDD1284  [6]
 LDCM0406  CL19 PaTu 8988t C404(0.33); C540(0.45); C557(0.45)  LDD1285  [6]
 LDCM0407  CL2 HCT 116 C404(1.00)  LDD0724  [6]
 LDCM0408  CL20 PaTu 8988t C404(0.34); C540(0.39); C557(0.39)  LDD1287  [6]
 LDCM0409  CL21 PaTu 8988t C404(0.36); C540(0.34); C557(0.34)  LDD1288  [6]
 LDCM0410  CL22 PaTu 8988t C404(0.33); C540(0.47); C557(0.47)  LDD1289  [6]
 LDCM0411  CL23 PaTu 8988t C404(0.33); C540(0.44); C557(0.44)  LDD1290  [6]
 LDCM0412  CL24 PaTu 8988t C404(0.54); C540(0.40); C557(0.40)  LDD1291  [6]
 LDCM0413  CL25 PaTu 8988t C404(0.44); C540(0.46); C557(0.46)  LDD1292  [6]
 LDCM0414  CL26 PaTu 8988t C404(0.27); C540(0.34); C557(0.34)  LDD1293  [6]
 LDCM0415  CL27 PaTu 8988t C404(0.36); C540(0.86); C557(0.86)  LDD1294  [6]
 LDCM0416  CL28 PaTu 8988t C404(0.37); C540(0.60); C557(0.60)  LDD1295  [6]
 LDCM0417  CL29 PaTu 8988t C404(0.36); C540(0.49); C557(0.49)  LDD1296  [6]
 LDCM0418  CL3 HCT 116 C404(1.26)  LDD0735  [6]
 LDCM0419  CL30 PaTu 8988t C404(0.41); C540(0.50); C557(0.50)  LDD1298  [6]
 LDCM0420  CL31 HCT 116 C404(0.78)  LDD0737  [6]
 LDCM0421  CL32 HCT 116 C404(0.80)  LDD0738  [6]
 LDCM0422  CL33 HCT 116 C404(0.60)  LDD0739  [6]
 LDCM0423  CL34 HCT 116 C404(0.46)  LDD0740  [6]
 LDCM0424  CL35 HCT 116 C404(0.45)  LDD0741  [6]
 LDCM0425  CL36 HCT 116 C404(0.58)  LDD0742  [6]
 LDCM0426  CL37 HCT 116 C404(0.43)  LDD0743  [6]
 LDCM0428  CL39 HCT 116 C404(0.50)  LDD0745  [6]
 LDCM0429  CL4 HCT 116 C404(0.84)  LDD0746  [6]
 LDCM0430  CL40 HCT 116 C404(0.48)  LDD0747  [6]
 LDCM0431  CL41 HCT 116 C404(0.78)  LDD0748  [6]
 LDCM0432  CL42 HCT 116 C404(0.35)  LDD0749  [6]
 LDCM0433  CL43 HCT 116 C404(0.44)  LDD0750  [6]
 LDCM0434  CL44 HCT 116 C404(0.62)  LDD0751  [6]
 LDCM0435  CL45 HCT 116 C404(0.56)  LDD0752  [6]
 LDCM0436  CL46 PaTu 8988t C626(0.86); C540(0.70); C557(0.70)  LDD1315  [6]
 LDCM0437  CL47 PaTu 8988t C626(0.73); C540(0.58); C557(0.58)  LDD1316  [6]
 LDCM0438  CL48 PaTu 8988t C626(0.80); C540(0.85); C557(0.85)  LDD1317  [6]
 LDCM0439  CL49 PaTu 8988t C626(0.91); C540(0.94); C557(0.94)  LDD1318  [6]
 LDCM0440  CL5 HCT 116 C404(0.93)  LDD0757  [6]
 LDCM0441  CL50 PaTu 8988t C626(1.00); C540(0.40); C557(0.40)  LDD1320  [6]
 LDCM0442  CL51 PaTu 8988t C626(0.97); C540(0.65); C557(0.65)  LDD1321  [6]
 LDCM0443  CL52 PaTu 8988t C626(0.77); C540(1.24); C557(1.24)  LDD1322  [6]
 LDCM0444  CL53 PaTu 8988t C626(0.77); C540(0.40); C557(0.40)  LDD1323  [6]
 LDCM0445  CL54 PaTu 8988t C626(0.75); C540(0.51); C557(0.51)  LDD1324  [6]
 LDCM0446  CL55 PaTu 8988t C626(0.67); C540(0.61); C557(0.61)  LDD1325  [6]
 LDCM0447  CL56 PaTu 8988t C626(0.26); C540(0.15); C557(0.15)  LDD1326  [6]
 LDCM0448  CL57 PaTu 8988t C626(0.87); C540(0.20); C557(0.20)  LDD1327  [6]
 LDCM0449  CL58 PaTu 8988t C626(0.71); C540(0.35); C557(0.35)  LDD1328  [6]
 LDCM0450  CL59 PaTu 8988t C626(0.78); C540(0.17); C557(0.17)  LDD1329  [6]
 LDCM0451  CL6 HCT 116 C404(0.76)  LDD0768  [6]
 LDCM0452  CL60 PaTu 8988t C626(0.80); C540(0.21); C557(0.21)  LDD1331  [6]
 LDCM0453  CL61 HEK-293T C540(1.53); C557(0.88)  LDD1051  [6]
 LDCM0454  CL62 HEK-293T C540(1.02); C557(1.22)  LDD1052  [6]
 LDCM0455  CL63 HEK-293T C540(1.09); C557(1.05)  LDD1053  [6]
 LDCM0456  CL64 HEK-293T C540(0.81); C557(0.80)  LDD1054  [6]
 LDCM0457  CL65 HEK-293T C540(0.96); C557(0.80)  LDD1055  [6]
 LDCM0458  CL66 HEK-293T C540(0.88); C557(0.94)  LDD1056  [6]
 LDCM0459  CL67 HEK-293T C540(0.73); C557(0.81)  LDD1057  [6]
 LDCM0460  CL68 HEK-293T C540(0.78); C557(1.03)  LDD1058  [6]
 LDCM0461  CL69 HEK-293T C540(0.99); C557(1.09)  LDD1059  [6]
 LDCM0462  CL7 HCT 116 C404(0.84)  LDD0779  [6]
 LDCM0463  CL70 HEK-293T C540(0.84); C557(0.88)  LDD1061  [6]
 LDCM0464  CL71 HEK-293T C540(0.89); C557(1.06)  LDD1062  [6]
 LDCM0465  CL72 HEK-293T C540(1.02); C557(1.45)  LDD1063  [6]
 LDCM0466  CL73 HEK-293T C540(0.79); C557(1.06)  LDD1064  [6]
 LDCM0467  CL74 HEK-293T C540(1.25); C557(1.34)  LDD1065  [6]
 LDCM0469  CL76 PaTu 8988t C404(0.77); C518(0.87); C626(1.04); C540(0.95)  LDD1348  [6]
 LDCM0470  CL77 PaTu 8988t C404(0.94); C518(0.71); C626(1.03); C540(0.74)  LDD1349  [6]
 LDCM0471  CL78 PaTu 8988t C404(0.79); C518(0.54); C626(1.09); C540(0.49)  LDD1350  [6]
 LDCM0472  CL79 PaTu 8988t C404(0.82); C518(0.68); C626(1.18); C540(0.81)  LDD1351  [6]
 LDCM0473  CL8 HCT 116 C404(0.56)  LDD0790  [6]
 LDCM0474  CL80 PaTu 8988t C404(0.73); C518(0.68); C626(1.03); C540(0.65)  LDD1353  [6]
 LDCM0475  CL81 PaTu 8988t C404(0.71); C518(0.60); C626(1.03); C540(0.49)  LDD1354  [6]
 LDCM0476  CL82 PaTu 8988t C404(0.98); C518(0.46); C626(1.21); C540(0.66)  LDD1355  [6]
 LDCM0477  CL83 PaTu 8988t C404(1.01); C518(0.53); C626(1.08); C540(0.56)  LDD1356  [6]
 LDCM0478  CL84 PaTu 8988t C404(0.60); C518(0.52); C626(1.15); C540(0.49)  LDD1357  [6]
 LDCM0479  CL85 PaTu 8988t C404(1.03); C518(0.59); C626(1.01); C540(0.85)  LDD1358  [6]
 LDCM0480  CL86 PaTu 8988t C404(0.94); C518(0.81); C626(1.04); C540(0.54)  LDD1359  [6]
 LDCM0481  CL87 PaTu 8988t C404(0.59); C518(0.65); C626(1.12); C540(0.45)  LDD1360  [6]
 LDCM0482  CL88 PaTu 8988t C404(0.70); C518(0.43); C626(0.98); C540(0.41)  LDD1361  [6]
 LDCM0483  CL89 PaTu 8988t C404(0.64); C518(1.09); C626(1.10); C540(0.75)  LDD1362  [6]
 LDCM0484  CL9 HCT 116 C404(1.04)  LDD0801  [6]
 LDCM0485  CL90 PaTu 8988t C404(0.52); C518(0.52); C626(1.16); C540(0.70)  LDD1364  [6]
 LDCM0486  CL91 HEK-293T C540(1.42); C557(1.05)  LDD1084  [6]
 LDCM0487  CL92 HEK-293T C540(1.39); C557(0.76)  LDD1085  [6]
 LDCM0488  CL93 HEK-293T C540(1.63); C557(0.90)  LDD1086  [6]
 LDCM0489  CL94 HEK-293T C540(1.25); C557(1.14)  LDD1087  [6]
 LDCM0490  CL95 HEK-293T C540(1.26); C557(1.23)  LDD1088  [6]
 LDCM0491  CL96 HEK-293T C540(1.49); C557(0.87)  LDD1089  [6]
 LDCM0492  CL97 HEK-293T C540(1.41); C557(0.68)  LDD1090  [6]
 LDCM0493  CL98 HEK-293T C540(0.88); C557(1.03)  LDD1091  [6]
 LDCM0494  CL99 HEK-293T C540(0.87); C557(1.03)  LDD1092  [6]
 LDCM0495  E2913 HEK-293T C626(0.90); C404(0.97); C255(0.95); C345(0.95)  LDD1698  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C29(1.21); C626(0.89)  LDD1702  [4]
 LDCM0625  F8 Ramos C29(1.21); C404(0.41); C361(1.94)  LDD2187  [13]
 LDCM0572  Fragment10 Ramos C29(2.97); C404(0.82); C361(0.67)  LDD2189  [13]
 LDCM0573  Fragment11 Ramos C29(0.75); C404(0.36)  LDD2190  [13]
 LDCM0574  Fragment12 Ramos C29(0.85); C404(1.52); C361(0.87)  LDD2191  [13]
 LDCM0575  Fragment13 Ramos C29(1.09); C361(0.62)  LDD2192  [13]
 LDCM0576  Fragment14 Ramos C29(0.24); C361(1.03)  LDD2193  [13]
 LDCM0579  Fragment20 Ramos C29(1.20); C404(0.85); C361(0.74)  LDD2194  [13]
 LDCM0580  Fragment21 Ramos C29(1.27); C404(1.68); C361(0.69)  LDD2195  [13]
 LDCM0582  Fragment23 Ramos C29(1.85); C404(0.86)  LDD2196  [13]
 LDCM0578  Fragment27 Ramos C29(1.13); C361(0.84)  LDD2197  [13]
 LDCM0586  Fragment28 Ramos C29(0.72); C361(0.57)  LDD2198  [13]
 LDCM0588  Fragment30 Ramos C29(1.18); C404(1.71); C361(1.02)  LDD2199  [13]
 LDCM0589  Fragment31 Ramos C29(0.73); C361(1.04)  LDD2200  [13]
 LDCM0590  Fragment32 Ramos C29(1.09); C404(0.63)  LDD2201  [13]
 LDCM0468  Fragment33 HEK-293T C540(0.95); C557(1.01)  LDD1066  [6]
 LDCM0596  Fragment38 Ramos C29(0.93); C404(3.30); C361(0.81)  LDD2203  [13]
 LDCM0566  Fragment4 Ramos C29(0.94)  LDD2184  [13]
 LDCM0427  Fragment51 HCT 116 C404(0.40)  LDD0744  [6]
 LDCM0610  Fragment52 Ramos C29(1.89); C361(0.73)  LDD2204  [13]
 LDCM0614  Fragment56 Ramos C29(2.23); C404(0.93)  LDD2205  [13]
 LDCM0569  Fragment7 Ramos C29(1.77); C404(0.67); C361(0.97)  LDD2186  [13]
 LDCM0571  Fragment9 Ramos C29(1.46); C404(1.10); C361(0.75)  LDD2188  [13]
 LDCM0022  KB02 HCT 116 C361(1.32)  LDD0080  [6]
 LDCM0023  KB03 HCT 116 C361(1.43)  LDD0081  [6]
 LDCM0024  KB05 HCT 116 C361(1.21)  LDD0082  [6]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C345(0.62)  LDD2089  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C345(1.19)  LDD2109  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C345(1.37)  LDD2127  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C345(1.44)  LDD2146  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C345(0.47)  LDD2150  [4]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
13 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578