Details of the Target

General Information of Target

Target ID LDTP11905
Target Name GDP-fucose protein O-fucosyltransferase 1 (POFUT1)
Gene Name POFUT1
Gene ID 23509
Synonyms
FUT12; KIAA0180; GDP-fucose protein O-fucosyltransferase 1; EC 2.4.1.221; Peptide-O-fucosyltransferase 1; O-FucT-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEQLLRAELRTATLRAFGGPGAGCISEGRAYDTDAGPVFVKVNRRTQARQMFEGEVASLE
ALRSTGLVRVPRPMKVIDLPGGGAAFVMEHLKMKSLSSQASKLGEQMADLHLYNQKLREK
LKEEENTVGRRGEGAEPQYVDKFGFHTVTCCGFIPQVNEWQDDWPTFFARHRLQAQLDLI
EKDYADREARELWSRLQVKIPDLFCGLEIVPALLHGDLWSGNVAEDDVGPIIYDPASFYG
HSEFELAIALMFGGFPRSFFTAYHRKIPKAPGFDQRLLLYQLFNYLNHWNHFGREYRSPS
LGTMRRLLK
Target Bioclass
Enzyme
Family
Glycosyltransferase 65 family
Subcellular location
Endoplasmic reticulum
Function
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).
Uniprot ID
Q9H488
Ensemble ID
ENST00000375730.3
HGNC ID
HGNC:14988

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO678 SNV: p.Q154E DBIA    Probe Info 
DU145 SNV: p.G377C DBIA    Probe Info 
JURKAT SNV: p.T359I .
NCIH2170 SNV: p.D216N .
NCIH358 Deletion: p.P193QfsTer10 DBIA    Probe Info 
RL952 SNV: p.V330A DBIA    Probe Info 
SKMEL5 SNV: p.P265S DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K295(8.56)  LDD0277  [1]
m-APA
 Probe Info  		15.00  LDD0403  [2]
DBIA
 Probe Info  		C267(1.44); C126(1.38)  LDD3310  [3]
BTD
 Probe Info  		C249(1.69); C140(1.02)  LDD1700  [4]
AHL-Pu-1
 Probe Info  		C126(2.39); C354(2.17); C267(4.96)  LDD0170  [5]
IPM
 Probe Info  		C126(1.75)  LDD1701  [4]
Acrolein
 Probe Info  		N.A.  LDD0221  [6]
5E-2FA
 Probe Info  		H260(0.00); H50(0.00)  LDD2235  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		C283(0.00); C126(0.00); C354(0.00)  LDD0038  [8]
IA-alkyne
 Probe Info  		C267(0.00); C283(0.00)  LDD0032  [9]
Lodoacetamide azide
 Probe Info  		C267(0.00); C249(0.00); C283(0.00); C126(0.00)  LDD0037  [8]
NAIA_4
 Probe Info  		N.A.  LDD2226  [10]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [6]
Methacrolein
 Probe Info  		N.A.  LDD0218  [6]
NAIA_5
 Probe Info  		C267(0.00); C249(0.00); C283(0.00); C126(0.00)  LDD2223  [10]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C140(0.89)  LDD2142  [4]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C140(0.76)  LDD2112  [4]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C126(1.01); C140(0.44)  LDD2095  [4]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C283(1.18)  LDD2130  [4]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C126(1.09); C140(1.63); C249(0.90); C283(0.96)  LDD2117  [4]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C140(1.27); C249(1.04); C267(0.83)  LDD2152  [4]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C140(0.71)  LDD2103  [4]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C140(0.68); C249(0.71)  LDD2132  [4]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C140(0.71); C249(0.99); C283(0.76)  LDD2131  [4]
 LDCM0025  4SU-RNA DM93 C126(2.39); C354(2.17); C267(4.96)  LDD0170  [5]
 LDCM0214  AC1 HEK-293T C126(1.02)  LDD1507  [12]
 LDCM0215  AC10 HEK-293T C267(0.93)  LDD0813  [13]
 LDCM0226  AC11 HEK-293T C267(1.08)  LDD0824  [13]
 LDCM0230  AC113 HEK-293T C267(1.16)  LDD0828  [13]
 LDCM0231  AC114 HEK-293T C267(0.85)  LDD0829  [13]
 LDCM0232  AC115 HEK-293T C267(0.77)  LDD0830  [13]
 LDCM0233  AC116 HEK-293T C267(0.67)  LDD0831  [13]
 LDCM0234  AC117 HEK-293T C267(1.07)  LDD0832  [13]
 LDCM0235  AC118 HEK-293T C267(0.65)  LDD0833  [13]
 LDCM0236  AC119 HEK-293T C267(0.71)  LDD0834  [13]
 LDCM0237  AC12 HEK-293T C267(0.93)  LDD0835  [13]
 LDCM0238  AC120 HEK-293T C267(0.79)  LDD0836  [13]
 LDCM0239  AC121 HEK-293T C267(1.04)  LDD0837  [13]
 LDCM0240  AC122 HEK-293T C267(0.78)  LDD0838  [13]
 LDCM0241  AC123 HEK-293T C267(0.75)  LDD0839  [13]
 LDCM0242  AC124 HEK-293T C267(0.75)  LDD0840  [13]
 LDCM0243  AC125 HEK-293T C267(0.78)  LDD0841  [13]
 LDCM0244  AC126 HEK-293T C267(0.86)  LDD0842  [13]
 LDCM0245  AC127 HEK-293T C267(0.92)  LDD0843  [13]
 LDCM0246  AC128 PaTu 8988t C267(0.70)  LDD1125  [13]
 LDCM0247  AC129 PaTu 8988t C267(0.51)  LDD1126  [13]
 LDCM0249  AC130 PaTu 8988t C267(1.64)  LDD1128  [13]
 LDCM0250  AC131 PaTu 8988t C267(0.87)  LDD1129  [13]
 LDCM0251  AC132 PaTu 8988t C267(0.72)  LDD1130  [13]
 LDCM0252  AC133 PaTu 8988t C267(0.25)  LDD1131  [13]
 LDCM0253  AC134 PaTu 8988t C267(0.60)  LDD1132  [13]
 LDCM0254  AC135 PaTu 8988t C267(0.27)  LDD1133  [13]
 LDCM0255  AC136 PaTu 8988t C267(0.50)  LDD1134  [13]
 LDCM0256  AC137 PaTu 8988t C267(0.31)  LDD1135  [13]
 LDCM0257  AC138 PaTu 8988t C267(1.13)  LDD1136  [13]
 LDCM0258  AC139 PaTu 8988t C267(0.75)  LDD1137  [13]
 LDCM0259  AC14 HEK-293T C267(0.97)  LDD0857  [13]
 LDCM0260  AC140 PaTu 8988t C267(0.92)  LDD1139  [13]
 LDCM0261  AC141 PaTu 8988t C267(1.03)  LDD1140  [13]
 LDCM0262  AC142 PaTu 8988t C267(0.62)  LDD1141  [13]
 LDCM0263  AC143 PaTu 8988t C267(1.03)  LDD1142  [13]
 LDCM0264  AC144 PaTu 8988t C267(1.22)  LDD1143  [13]
 LDCM0265  AC145 PaTu 8988t C267(0.93)  LDD1144  [13]
 LDCM0266  AC146 PaTu 8988t C267(1.08)  LDD1145  [13]
 LDCM0267  AC147 PaTu 8988t C267(1.14)  LDD1146  [13]
 LDCM0268  AC148 PaTu 8988t C267(0.88)  LDD1147  [13]
 LDCM0269  AC149 PaTu 8988t C267(0.86)  LDD1148  [13]
 LDCM0270  AC15 HEK-293T C267(0.91)  LDD0868  [13]
 LDCM0271  AC150 PaTu 8988t C267(1.22)  LDD1150  [13]
 LDCM0272  AC151 PaTu 8988t C267(0.52)  LDD1151  [13]
 LDCM0273  AC152 PaTu 8988t C267(0.86)  LDD1152  [13]
 LDCM0274  AC153 PaTu 8988t C267(0.88)  LDD1153  [13]
 LDCM0621  AC154 PaTu 8988t C267(0.67)  LDD2166  [13]
 LDCM0622  AC155 PaTu 8988t C267(0.82)  LDD2167  [13]
 LDCM0623  AC156 PaTu 8988t C267(0.83)  LDD2168  [13]
 LDCM0624  AC157 PaTu 8988t C267(0.96)  LDD2169  [13]
 LDCM0276  AC17 PaTu 8988t C267(1.26)  LDD1155  [13]
 LDCM0277  AC18 PaTu 8988t C267(1.11)  LDD1156  [13]
 LDCM0278  AC19 PaTu 8988t C267(1.30)  LDD1157  [13]
 LDCM0279  AC2 HEK-293T C126(0.93); C249(0.97)  LDD1518  [12]
 LDCM0280  AC20 PaTu 8988t C267(0.90)  LDD1159  [13]
 LDCM0281  AC21 PaTu 8988t C267(0.83)  LDD1160  [13]
 LDCM0282  AC22 PaTu 8988t C267(0.55)  LDD1161  [13]
 LDCM0283  AC23 PaTu 8988t C267(0.81)  LDD1162  [13]
 LDCM0284  AC24 PaTu 8988t C267(1.25)  LDD1163  [13]
 LDCM0285  AC25 PaTu 8988t C267(0.95)  LDD1164  [13]
 LDCM0286  AC26 PaTu 8988t C267(0.83)  LDD1165  [13]
 LDCM0287  AC27 PaTu 8988t C267(1.00)  LDD1166  [13]
 LDCM0288  AC28 PaTu 8988t C267(1.76)  LDD1167  [13]
 LDCM0289  AC29 PaTu 8988t C267(0.98)  LDD1168  [13]
 LDCM0290  AC3 HEK-293T C126(0.95)  LDD1529  [12]
 LDCM0291  AC30 PaTu 8988t C267(0.96)  LDD1170  [13]
 LDCM0292  AC31 PaTu 8988t C267(1.09)  LDD1171  [13]
 LDCM0293  AC32 PaTu 8988t C267(1.19)  LDD1172  [13]
 LDCM0294  AC33 PaTu 8988t C267(1.12)  LDD1173  [13]
 LDCM0295  AC34 PaTu 8988t C267(0.88)  LDD1174  [13]
 LDCM0296  AC35 PaTu 8988t C267(0.83)  LDD1175  [13]
 LDCM0297  AC36 PaTu 8988t C267(0.88)  LDD1176  [13]
 LDCM0298  AC37 PaTu 8988t C267(1.01)  LDD1177  [13]
 LDCM0299  AC38 PaTu 8988t C267(0.99)  LDD1178  [13]
 LDCM0300  AC39 PaTu 8988t C267(1.71)  LDD1179  [13]
 LDCM0301  AC4 HEK-293T C126(0.93); C249(1.03)  LDD1540  [12]
 LDCM0302  AC40 PaTu 8988t C267(1.01)  LDD1181  [13]
 LDCM0303  AC41 PaTu 8988t C267(1.03)  LDD1182  [13]
 LDCM0304  AC42 PaTu 8988t C267(1.64)  LDD1183  [13]
 LDCM0305  AC43 PaTu 8988t C267(1.15)  LDD1184  [13]
 LDCM0306  AC44 PaTu 8988t C267(0.86)  LDD1185  [13]
 LDCM0307  AC45 PaTu 8988t C267(0.90)  LDD1186  [13]
 LDCM0308  AC46 PaTu 8988t C267(1.19)  LDD1187  [13]
 LDCM0309  AC47 PaTu 8988t C267(0.67)  LDD1188  [13]
 LDCM0310  AC48 PaTu 8988t C267(1.07)  LDD1189  [13]
 LDCM0311  AC49 PaTu 8988t C267(1.36)  LDD1190  [13]
 LDCM0312  AC5 HEK-293T C126(1.11); C249(0.99)  LDD1551  [12]
 LDCM0313  AC50 PaTu 8988t C267(0.76)  LDD1192  [13]
 LDCM0314  AC51 PaTu 8988t C267(0.27)  LDD1193  [13]
 LDCM0315  AC52 PaTu 8988t C267(1.47)  LDD1194  [13]
 LDCM0316  AC53 PaTu 8988t C267(0.77)  LDD1195  [13]
 LDCM0317  AC54 PaTu 8988t C267(0.84)  LDD1196  [13]
 LDCM0318  AC55 PaTu 8988t C267(1.06)  LDD1197  [13]
 LDCM0319  AC56 PaTu 8988t C267(0.96)  LDD1198  [13]
 LDCM0320  AC57 HEK-293T C267(1.35)  LDD0918  [13]
 LDCM0321  AC58 HEK-293T C267(1.61)  LDD0919  [13]
 LDCM0322  AC59 HEK-293T C267(1.61)  LDD0920  [13]
 LDCM0323  AC6 HEK-293T C267(0.88)  LDD0921  [13]
 LDCM0324  AC60 HEK-293T C267(0.95)  LDD0922  [13]
 LDCM0325  AC61 HEK-293T C267(1.35)  LDD0923  [13]
 LDCM0326  AC62 HEK-293T C267(1.64)  LDD0924  [13]
 LDCM0327  AC63 HEK-293T C267(1.04)  LDD0925  [13]
 LDCM0328  AC64 HEK-293T C267(1.85)  LDD0926  [13]
 LDCM0329  AC65 HEK-293T C267(1.14)  LDD0927  [13]
 LDCM0330  AC66 HEK-293T C267(1.35)  LDD0928  [13]
 LDCM0331  AC67 HEK-293T C267(0.97)  LDD0929  [13]
 LDCM0334  AC7 HEK-293T C267(0.89)  LDD0932  [13]
 LDCM0345  AC8 HEK-293T C267(0.84)  LDD0943  [13]
 LDCM0545  Acetamide MDA-MB-231 C140(0.32); C249(0.43); C267(0.53)  LDD2138  [4]
 LDCM0520  AKOS000195272 MDA-MB-231 C249(0.79)  LDD2113  [4]
 LDCM0248  AKOS034007472 HEK-293T C267(1.05)  LDD0846  [13]
 LDCM0356  AKOS034007680 HEK-293T C267(0.92)  LDD0954  [13]
 LDCM0275  AKOS034007705 HEK-293T C267(0.89)  LDD0873  [13]
 LDCM0156  Aniline NCI-H1299 15.00  LDD0403  [2]
 LDCM0498  BS-3668 MDA-MB-231 C126(0.82); C140(0.43); C283(0.96); C267(0.60)  LDD2091  [4]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [6]
 LDCM0367  CL1 PaTu 8988t C267(0.75)  LDD1246  [13]
 LDCM0368  CL10 PaTu 8988t C267(0.61)  LDD1247  [13]
 LDCM0369  CL100 HEK-293T C126(0.92)  LDD1573  [12]
 LDCM0370  CL101 HEK-293T C267(0.85)  LDD0968  [13]
 LDCM0371  CL102 HEK-293T C267(0.87)  LDD0969  [13]
 LDCM0372  CL103 HEK-293T C267(0.84)  LDD0970  [13]
 LDCM0373  CL104 HEK-293T C267(0.85)  LDD0971  [13]
 LDCM0374  CL105 PaTu 8988t C267(1.31)  LDD1253  [13]
 LDCM0375  CL106 PaTu 8988t C267(1.65)  LDD1254  [13]
 LDCM0376  CL107 PaTu 8988t C267(2.43)  LDD1255  [13]
 LDCM0377  CL108 PaTu 8988t C267(1.15)  LDD1256  [13]
 LDCM0378  CL109 PaTu 8988t C267(1.33)  LDD1257  [13]
 LDCM0379  CL11 PaTu 8988t C267(0.53)  LDD1258  [13]
 LDCM0380  CL110 PaTu 8988t C267(1.09)  LDD1259  [13]
 LDCM0381  CL111 PaTu 8988t C267(0.66)  LDD1260  [13]
 LDCM0382  CL112 PaTu 8988t C267(1.10)  LDD1261  [13]
 LDCM0383  CL113 PaTu 8988t C267(0.93)  LDD1262  [13]
 LDCM0384  CL114 PaTu 8988t C267(1.21)  LDD1263  [13]
 LDCM0385  CL115 PaTu 8988t C267(1.17)  LDD1264  [13]
 LDCM0386  CL116 PaTu 8988t C267(1.07)  LDD1265  [13]
 LDCM0387  CL117 PaTu 8988t C267(1.14)  LDD1266  [13]
 LDCM0388  CL118 PaTu 8988t C267(1.10)  LDD1267  [13]
 LDCM0389  CL119 PaTu 8988t C267(0.97)  LDD1268  [13]
 LDCM0390  CL12 PaTu 8988t C267(0.77)  LDD1269  [13]
 LDCM0391  CL120 PaTu 8988t C267(0.85)  LDD1270  [13]
 LDCM0392  CL121 PaTu 8988t C267(1.84)  LDD1271  [13]
 LDCM0393  CL122 PaTu 8988t C267(1.21)  LDD1272  [13]
 LDCM0394  CL123 PaTu 8988t C267(1.02)  LDD1273  [13]
 LDCM0395  CL124 PaTu 8988t C267(0.92)  LDD1274  [13]
 LDCM0396  CL125 HEK-293T C267(1.53)  LDD0994  [13]
 LDCM0397  CL126 HEK-293T C267(1.19)  LDD0995  [13]
 LDCM0398  CL127 HEK-293T C267(1.32)  LDD0996  [13]
 LDCM0399  CL128 HEK-293T C267(0.91)  LDD0997  [13]
 LDCM0400  CL13 PaTu 8988t C267(0.19)  LDD1279  [13]
 LDCM0401  CL14 PaTu 8988t C267(0.18)  LDD1280  [13]
 LDCM0402  CL15 PaTu 8988t C267(1.09)  LDD1281  [13]
 LDCM0403  CL16 HEK-293T C267(1.02)  LDD1001  [13]
 LDCM0404  CL17 HEK-293T C267(1.10)  LDD1002  [13]
 LDCM0405  CL18 HEK-293T C267(1.04)  LDD1003  [13]
 LDCM0406  CL19 HEK-293T C267(0.98)  LDD1004  [13]
 LDCM0407  CL2 PaTu 8988t C267(0.83)  LDD1286  [13]
 LDCM0408  CL20 HEK-293T C267(1.06)  LDD1006  [13]
 LDCM0409  CL21 HEK-293T C267(1.00)  LDD1007  [13]
 LDCM0410  CL22 HEK-293T C267(1.18)  LDD1008  [13]
 LDCM0411  CL23 HEK-293T C267(1.20)  LDD1009  [13]
 LDCM0412  CL24 HEK-293T C267(1.31)  LDD1010  [13]
 LDCM0413  CL25 HEK-293T C267(1.12)  LDD1011  [13]
 LDCM0414  CL26 HEK-293T C267(1.19)  LDD1012  [13]
 LDCM0415  CL27 HEK-293T C267(1.00)  LDD1013  [13]
 LDCM0416  CL28 HEK-293T C267(1.08)  LDD1014  [13]
 LDCM0417  CL29 HEK-293T C267(1.12)  LDD1015  [13]
 LDCM0418  CL3 PaTu 8988t C267(0.91)  LDD1297  [13]
 LDCM0419  CL30 HEK-293T C267(1.20)  LDD1017  [13]
 LDCM0420  CL31 PaTu 8988t C267(0.91)  LDD1299  [13]
 LDCM0421  CL32 PaTu 8988t C267(0.79)  LDD1300  [13]
 LDCM0422  CL33 PaTu 8988t C267(0.77)  LDD1301  [13]
 LDCM0423  CL34 PaTu 8988t C267(0.74)  LDD1302  [13]
 LDCM0424  CL35 PaTu 8988t C267(0.60)  LDD1303  [13]
 LDCM0425  CL36 PaTu 8988t C267(0.81)  LDD1304  [13]
 LDCM0426  CL37 PaTu 8988t C267(0.49)  LDD1305  [13]
 LDCM0428  CL39 PaTu 8988t C267(0.72)  LDD1307  [13]
 LDCM0429  CL4 PaTu 8988t C267(1.00)  LDD1308  [13]
 LDCM0430  CL40 PaTu 8988t C267(0.88)  LDD1309  [13]
 LDCM0431  CL41 PaTu 8988t C267(0.67)  LDD1310  [13]
 LDCM0432  CL42 PaTu 8988t C267(0.36)  LDD1311  [13]
 LDCM0433  CL43 PaTu 8988t C267(0.11)  LDD1312  [13]
 LDCM0434  CL44 PaTu 8988t C267(0.69)  LDD1313  [13]
 LDCM0435  CL45 PaTu 8988t C267(1.04)  LDD1314  [13]
 LDCM0436  CL46 PaTu 8988t C267(0.89)  LDD1315  [13]
 LDCM0437  CL47 PaTu 8988t C267(0.79)  LDD1316  [13]
 LDCM0438  CL48 PaTu 8988t C267(1.22)  LDD1317  [13]
 LDCM0439  CL49 PaTu 8988t C267(1.07)  LDD1318  [13]
 LDCM0440  CL5 PaTu 8988t C267(0.39)  LDD1319  [13]
 LDCM0441  CL50 PaTu 8988t C267(0.87)  LDD1320  [13]
 LDCM0442  CL51 PaTu 8988t C267(0.88)  LDD1321  [13]
 LDCM0443  CL52 PaTu 8988t C267(0.98)  LDD1322  [13]
 LDCM0444  CL53 PaTu 8988t C267(0.82)  LDD1323  [13]
 LDCM0445  CL54 PaTu 8988t C267(0.87)  LDD1324  [13]
 LDCM0446  CL55 PaTu 8988t C267(0.77)  LDD1325  [13]
 LDCM0447  CL56 PaTu 8988t C267(0.99)  LDD1326  [13]
 LDCM0448  CL57 PaTu 8988t C267(1.05)  LDD1327  [13]
 LDCM0449  CL58 PaTu 8988t C267(0.93)  LDD1328  [13]
 LDCM0450  CL59 PaTu 8988t C267(1.47)  LDD1329  [13]
 LDCM0451  CL6 PaTu 8988t C267(0.71)  LDD1330  [13]
 LDCM0452  CL60 PaTu 8988t C267(0.96)  LDD1331  [13]
 LDCM0453  CL61 PaTu 8988t C267(1.12)  LDD1332  [13]
 LDCM0454  CL62 PaTu 8988t C267(0.76)  LDD1333  [13]
 LDCM0455  CL63 PaTu 8988t C267(0.69)  LDD1334  [13]
 LDCM0456  CL64 PaTu 8988t C267(0.91)  LDD1335  [13]
 LDCM0457  CL65 PaTu 8988t C267(1.22)  LDD1336  [13]
 LDCM0458  CL66 PaTu 8988t C267(0.72)  LDD1337  [13]
 LDCM0459  CL67 PaTu 8988t C267(0.53)  LDD1338  [13]
 LDCM0460  CL68 PaTu 8988t C267(0.38)  LDD1339  [13]
 LDCM0461  CL69 PaTu 8988t C267(0.49)  LDD1340  [13]
 LDCM0462  CL7 PaTu 8988t C267(0.42)  LDD1341  [13]
 LDCM0463  CL70 PaTu 8988t C267(0.63)  LDD1342  [13]
 LDCM0464  CL71 PaTu 8988t C267(0.99)  LDD1343  [13]
 LDCM0465  CL72 PaTu 8988t C267(0.89)  LDD1344  [13]
 LDCM0466  CL73 PaTu 8988t C267(0.60)  LDD1345  [13]
 LDCM0467  CL74 PaTu 8988t C267(0.86)  LDD1346  [13]
 LDCM0469  CL76 HEK-293T C267(1.89)  LDD1067  [13]
 LDCM0470  CL77 HEK-293T C267(1.90)  LDD1068  [13]
 LDCM0471  CL78 HEK-293T C267(2.63)  LDD1069  [13]
 LDCM0472  CL79 HEK-293T C267(1.78)  LDD1070  [13]
 LDCM0473  CL8 PaTu 8988t C267(0.61)  LDD1352  [13]
 LDCM0474  CL80 HEK-293T C267(1.33)  LDD1072  [13]
 LDCM0475  CL81 HEK-293T C267(1.58)  LDD1073  [13]
 LDCM0476  CL82 HEK-293T C267(1.33)  LDD1074  [13]
 LDCM0477  CL83 HEK-293T C267(1.28)  LDD1075  [13]
 LDCM0478  CL84 HEK-293T C267(1.54)  LDD1076  [13]
 LDCM0479  CL85 HEK-293T C267(1.61)  LDD1077  [13]
 LDCM0480  CL86 HEK-293T C267(1.75)  LDD1078  [13]
 LDCM0481  CL87 HEK-293T C267(1.64)  LDD1079  [13]
 LDCM0482  CL88 HEK-293T C267(1.61)  LDD1080  [13]
 LDCM0483  CL89 HEK-293T C267(1.86)  LDD1081  [13]
 LDCM0484  CL9 PaTu 8988t C267(0.60)  LDD1363  [13]
 LDCM0485  CL90 HEK-293T C267(1.64)  LDD1083  [13]
 LDCM0486  CL91 HEK-293T C126(0.95)  LDD1689  [12]
 LDCM0487  CL92 HEK-293T C126(0.91); C249(1.10)  LDD1690  [12]
 LDCM0488  CL93 HEK-293T C126(0.95); C249(1.26)  LDD1691  [12]
 LDCM0490  CL95 HEK-293T C126(0.94); C249(1.14)  LDD1693  [12]
 LDCM0491  CL96 HEK-293T C126(0.98); C267(1.00); C249(1.30); C283(1.06)  LDD1694  [12]
 LDCM0492  CL97 HEK-293T C126(1.14); C267(0.92); C249(1.00)  LDD1695  [12]
 LDCM0493  CL98 HEK-293T C126(1.04); C249(1.08)  LDD1696  [12]
 LDCM0494  CL99 HEK-293T C126(0.94); C267(1.19)  LDD1697  [12]
 LDCM0495  E2913 HEK-293T C126(0.90); C267(1.15)  LDD1698  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C126(1.77)  LDD1702  [4]
 LDCM0468  Fragment33 PaTu 8988t C267(1.21)  LDD1347  [13]
 LDCM0427  Fragment51 PaTu 8988t C267(0.52)  LDD1306  [13]
 LDCM0107  IAA HeLa N.A.  LDD0221  [6]
 LDCM0022  KB02 HEK-293T C267(0.97)  LDD1492  [12]
 LDCM0023  KB03 HEK-293T C267(1.11)  LDD1497  [12]
 LDCM0024  KB05 COLO792 C267(1.44); C126(1.38)  LDD3310  [3]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C140(0.57)  LDD2102  [4]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C140(0.44); C249(0.64); C283(0.93); C267(0.68)  LDD2121  [4]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C249(0.65); C283(1.03); C267(0.57)  LDD2089  [4]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C140(0.74)  LDD2092  [4]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C249(1.12)  LDD2093  [4]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C140(0.74)  LDD2094  [4]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C249(0.79)  LDD2097  [4]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C140(0.77); C249(0.51)  LDD2098  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C249(0.83)  LDD2099  [4]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C140(0.72); C249(0.49)  LDD2100  [4]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C249(0.66)  LDD2104  [4]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C140(1.03)  LDD2105  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C140(0.99); C249(0.79); C283(0.90); C267(0.81)  LDD2107  [4]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C140(0.73)  LDD2108  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C126(0.93); C140(1.00); C249(0.84); C283(1.19)  LDD2109  [4]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C126(1.22); C140(1.03); C249(0.93)  LDD2111  [4]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C140(0.63)  LDD2114  [4]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C249(0.41)  LDD2115  [4]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C126(1.17); C140(0.99); C249(1.52); C267(1.66)  LDD2119  [4]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C140(0.81)  LDD2120  [4]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C140(0.66)  LDD2122  [4]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C126(1.10); C140(1.29); C249(0.67); C283(0.87)  LDD2123  [4]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C126(0.90); C140(0.84); C249(0.86)  LDD2125  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C126(1.55); C140(2.64); C249(0.77); C283(0.96)  LDD2127  [4]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C140(0.85); C249(0.86)  LDD2128  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C140(1.08); C249(1.05)  LDD2129  [4]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C249(0.59)  LDD2133  [4]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C249(0.72); C267(0.94)  LDD2134  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C126(1.44); C140(1.13); C249(0.82); C267(1.00)  LDD2135  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C140(1.07); C249(0.88); C283(1.13); C267(1.66)  LDD2136  [4]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C126(1.02); C140(1.26); C249(0.66); C283(0.94)  LDD2137  [4]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C249(1.69); C140(1.02)  LDD1700  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C140(0.73); C249(0.64); C283(0.95); C267(0.76)  LDD2140  [4]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C140(0.58); C249(0.63)  LDD2141  [4]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C140(0.98); C249(0.89)  LDD2143  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C126(1.43); C283(1.45)  LDD2144  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C140(1.25); C249(0.74); C267(0.92)  LDD2146  [4]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C249(0.64)  LDD2148  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C140(0.40); C283(0.93); C267(0.62)  LDD2150  [4]

References

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Mass spectrometry data entry: PXD028270
2 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
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Mass spectrometry data entry: PXD044402
13 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.