Details of the Target

General Information of Target

Target ID LDTP11210
Target Name Transmembrane protein 43 (TMEM43)
Gene Name TMEM43
Gene ID 79188
Synonyms
Transmembrane protein 43; Protein LUMA
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDETSPLVSPERAQPPDYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPGSPGHDRERQP
LLDRARGAAAQGQTQTVAAQAQALAAQAAAAAHAAQAHRERNEFPEDPEFEAVVRQAELA
IERCIFPERIYQGSSGSYFVKDPQGRIIAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFG
RDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEK
VPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVL
DYIIRNTDRGNDNWLIKYDCPMDSSSSRDTDWVVVKEPVIKVAAIDNGLAFPLKHPDSWR
AYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFVKDLEEDLYELFKKDPGFDRGQFHKQI
AVMRGQILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSSSESYTQSFQSRKPFFSWW
Target Bioclass
Other
Family
TMEM43 family
Subcellular location
Endoplasmic reticulum membrane
Function
May have an important role in maintaining nuclear envelope structure by organizing protein complexes at the inner nuclear membrane. Required for retaining emerin at the inner nuclear membrane. Plays a role in the modulation of innate immune signaling through the cGAS-STING pathway by interacting with RNF26. In addition, functions as a critical signaling component in mediating NF-kappa-B activation by acting downstream of EGFR and upstream of CARD10. Contributes to passive conductance current in cochlear glia-like supporting cells, mediated by gap junctions and necessary for hearing and speech discrimination.
Uniprot ID
Q9BTV4
Ensemble ID
ENST00000306077.5
HGNC ID
HGNC:28472

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ONAyne
 Probe Info  		K232(9.19)  LDD0274  [1]
STPyne
 Probe Info  		K18(9.09); K199(0.69); K210(6.67)  LDD0277  [1]
Probe 1
 Probe Info  		Y133(13.74); Y233(11.22)  LDD3495  [2]
D5yne
 Probe Info  		1.52  LDD0055  [3]
Jackson_14
 Probe Info  		2.13  LDD0123  [4]
ATP probe
 Probe Info  		K232(0.00); K210(0.00)  LDD0199  [5]
NHS
 Probe Info  		N.A.  LDD0010  [6]
SF
 Probe Info  		N.A.  LDD0028  [7]
Ox-W18
 Probe Info  		W124(0.00); W151(0.00)  LDD2175  [8]
1c-yne
 Probe Info  		N.A.  LDD0228  [9]
Acrolein
 Probe Info  		H215(0.00); H298(0.00)  LDD0217  [10]
PAL-AfBPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C040
 Probe Info  		7.31  LDD1740  [11]
C055
 Probe Info  		27.28  LDD1752  [11]
C094
 Probe Info  		26.91  LDD1785  [11]
C228
 Probe Info  		14.72  LDD1901  [11]
C431
 Probe Info  		14.62  LDD2086  [11]
FFF probe11
 Probe Info  		18.92  LDD0471  [12]
FFF probe13
 Probe Info  		14.77  LDD0475  [12]
FFF probe14
 Probe Info  		12.15  LDD0477  [12]
FFF probe2
 Probe Info  		18.16  LDD0463  [12]
FFF probe3
 Probe Info  		20.00  LDD0464  [12]
JN0003
 Probe Info  		20.00  LDD0469  [12]
VE-P
 Probe Info  		N.A.  LDD0396  [13]
OEA-DA
 Probe Info  		7.80  LDD0046  [14]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H215(0.00); H90(0.00)  LDD0222  [10]
 LDCM0095  DC-LC3IN-D5 HeLa 1.52  LDD0055  [3]
 LDCM0107  IAA HeLa H90(0.00); H215(0.00)  LDD0221  [10]
 LDCM0109  NEM HeLa H90(0.00); H215(0.00); H298(0.00)  LDD0223  [10]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 2.13  LDD0123  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin carboxyl-terminal hydrolase 25 (USP25) Peptidase C19 family Q9UHP3
E3 ubiquitin-protein ligase SIAH1 (SIAH1) SINA (Seven in absentia) family Q8IUQ4
ADP-ribosylation factor-like protein 13B (ARL13B) Arf family Q3SXY8
Peptidyl-prolyl cis-trans isomerase FKBP8 (FKBP8) . Q14318
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Synapse differentiation-inducing gene protein 1 (SYNDIG1) CD225/Dispanin family Q9H7V2
Huntingtin (HTT) Huntingtin family P42858
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Uteroglobin (SCGB1A1) Secretoglobin family P11684
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Apolipoprotein A-V (APOA5) Apolipoprotein A1/A4/E family Q6Q788
CDGSH iron-sulfur domain-containing protein 2 (CISD2) CISD protein family Q8N5K1
Protein FAM209A (FAM209A) FAM209 family Q5JX71
Transmembrane protein 43 (TMEM43) TMEM43 family Q9BTV4
Zinc finger CCHC domain-containing protein 12 (ZCCHC12) ZCCHC12 family Q6PEW1
Emerin (EMD) . P50402
GRAM domain-containing protein 2B (GRAMD2B) . Q96HH9

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Inhibition of Autophagy by a Small Molecule through Covalent Modification of the LC3 Protein. Angew Chem Int Ed Engl. 2021 Dec 6;60(50):26105-26114. doi: 10.1002/anie.202109464. Epub 2021 Nov 5.
Mass spectrometry data entry: PXD026874
4 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
8 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
9 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
10 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
11 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
12 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
13 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
14 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570